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Authors requiring further information regarding Elsevier’s archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright Author's personal copy Crop Protection 41 (2012) 77e87 Contents lists available at SciVerse ScienceDirect Crop Protection journal homepage: www.elsevier.com/locate/cropro Spray deposition and distribution in a bay laurel crop as affected by nozzle type, air assistance and spray direction when using vertical spray booms Dieter Foqué a, *, Jan G. Pieters b, David Nuyttens a, * a b Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Agricultural Engineering, Burg. Van Gansberghelaan 115, bus 1, 9820 Merelbeke, Belgium Ghent University, Department of Biosystems Engineering, Faculty of Bioscience Engineering, Coupure Links 653, 9000 Ghent, Belgium a r t i c l e i n f o a b s t r a c t Article history: Received 16 December 2011 Received in revised form 14 May 2012 Accepted 20 May 2012 In Flanders, greenhouse growers predominantly use handheld sprayers instead of spray boom equipment. Nevertheless, handheld sprayers have several drawbacks. Growers increasingly recognize the advantages of spray boom equipment, which has resulted in increasing adoption of spray boom systems. Some growers still doubt the efficacy of spray boom systems, however, while others have questions about their use. In this study, we aimed to address both doubts and questions by optimizing a vertical spray boom application for a bay laurel crop using air support and spray angling. Spray deposition on the stem and the upper and lower side of the leaves was measured using mineral chelates as tracers at twenty collector positions in the top and bottom as well as in the front, back, left and right zone of the plant. Four plant repetitions were used for every spray event. Nine different application techniques were tested in three repetitions in laboratory conditions using a fully automated spray system. The effect of nozzle type, angled nozzles, air support and spraying in two passes with an opposite direction was evaluated. The experiments showed that collector position and application technique had a significant effect on deposition. However, angling the spray, using air support or spraying in two consecutive passes with an opposite direction did not result in a higher deposition or penetration capacity compared with the standard vertical spray boom. Nevertheless, some techniques might still result in a higher bioefficacy due to a more homogenous liquid distribution. The use of a vertical spray boom is a promising technique for safe and efficient application of plant protection products in a vertical crop. Nozzle choice, spray boom setting, spray distance and the air speed of air assisted sprayers require careful consideration, however. Of the tested techniques, the applications made with an extended range standard flat fan nozzle without air support, directed straight toward the crop and used with a fixed spray distance of 30 cm to the stem generally produced the best spray results in the considered, conically pruned bay laurel crop. Ó 2012 Elsevier Ltd. All rights reserved. Keywords: Spray application technique Nozzle type Spray angling Spraying in 2 passes Spray distribution Ornamental crops 1. Introduction In 2006, we began researching the optimization of the spray equipment and technology used in various ornamental crops in Flanders (Belgium) in collaboration with the Research Center for Ornamental Plants (PCS). We first surveyed several greenhouse growers about the spray equipment and technology they used for their plant protection needs. That enquiry revealed that most of them use spray guns and spray lances for crop protection and that persistent pests and diseases are common. We also gathered * Corresponding authors. Tel.: þ32 9 272 28 00; fax: þ32 9 272 28 01. E-mail addresses: [email protected] (D. Foqué), david.nuyttens@ ilvo.vlaanderen.be (D. Nuyttens). 0261-2194/$ e see front matter Ó 2012 Elsevier Ltd. All rights reserved. doi:10.1016/j.cropro.2012.05.020 information on the spray parameters used by the growers. Most of them use their equipment at high pressures (up to 50 bar) and low travelling speeds, which results in relatively high spray volumes (2000 l ha1 and more). These results were similar to data gathered from surveys in 2004 (Braekman and Sonck, 2008; Goossens et al., 2004) and 2005 (Vissers, pers. Comm.). Many growers still believe that high spray volumes or spray pressures assure good plant protection (Braekman and Sonck, 2008; Goossens et al., 2004). However, both older and newer surveys (Braekman and Sonck, 2008) indicated that this approach does not give the desired results. Furthermore, the growers’ opinion disagrees with literature. Derksen et al. (2001) reported that high-pressure sprays had a lower penetration capacity and did not result in a better coverage when compared to low-pressure sprays. Sánchez-Hermosilla et al. (2003) showed that the spray deposition of a vertical boom in Author's personal copy 78 D. Foqué et al. / Crop Protection 41 (2012) 77e87 a tomato crop after application at 750 l ha1 and a spray pressure of 15 bar was comparable to that of a spray gun application with a spray volume of 2000 l ha1 and a spray pressure of 38 bar. van Os et al. (2005) found that decreasing the spray pressure to 5 bar provided adequate depositions and lead to reduced chemical losses in a tomato crop. Prokop and Veverka (2006) proved that poor leaf coverage can only be partly compensated by higher spray volumes. Braekman et al. (2009, 2010) demonstrated that higher relative deposition can be obtained with a spray boom compared to a spray gun, regardless of the nozzle type used, and that the spray volume can be reduced by using a spray boom instead of a spray gun or lance. Additionally, Braekman et al. (2010) showed that using small extended-range flat fan nozzles at a pressure above the recommended pressure range resulted in significantly lower depositions, especially inside the canopy. Sanchez-Hermosilla et al. (2011) concluded that spray gun applications often result in inadequate penetration in the canopy, heavy losses to the ground due to leaf run-off, and insufficient deposition on the underside of the leaves. These studies all indicate that using a spray gun at a higher spray volume and pressure does not result in improved pest control. Nevertheless, when confronted with unresponsive or resistant plagues and diseases, growers still tend to increase their spray frequency, spray pressure, and spray volume. In some cases, they also repeatedly use the same active ingredients. These practices only increase the risk of increased resistance of pest and diseases. Furthermore, since most plant protection products authorized for ornamentals in Belgium prescribe the dose needed as a concentration (e.g., 50 g 100 l spray volume, (Anon., 2012a)), these high spray volumes mean higher potential exposure risks for the applicator and the environment (Bjugstad and Torgrimsen, 1996; Hughes et al., 2006; Machera et al., 2009; Vidal et al., 2002). In addition to the exposure risks and higher pressures and application rates used, spray gun techniques are also known to result in less uniform spray results and higher labor costs when compared to spray boom equipment (Braekman et al., 2009, 2010; González et al., 2009; Knewitz et al., 2003; Langenakens et al., 2002; Molto et al., 2000; Nuyttens et al., 2004, 2009b; SánchezHermosilla et al., 2003, 2011; Subramanian et al., 2005; Vidal et al., 2002). Growers are increasingly recognizing the advantages of spray boom systems and many are switching to spray boom systems (Braekman and Sonck, 2008; Goossens et al., 2004). Many growers have questions about spray boom systems, e.g. which application technique to use, the maintenance of the selected equipment and optimal settings for it, choosing the right nozzle type for their crop, etc. Still others doubt the efficacy of spray boom systems. Many growers also believe that spray boom systems are expensive, fear that they would be difficult to use in narrow passageways between plant rows, and doubt that they would be applicable given the diversity in plants and production systems found within the same company. To address some of these questions, several spray boom prototypes have been built and assessed in field (Braekman et al., 2009) and laboratory trials (Foqué et al., 2012). Additionally, the deposition of spray liquids using spray boom techniques was optimized for 2 important ornamental crops grown in Flanders: ivy (Hedera sp.) and bay laurel (Laurus nobilis). These tests were carried out under laboratory conditions in ILVO’s Spray Tech Lab (Anon., 2012b). For ivy (Foqué and Nuyttens, 2011a, 2011b; Foqué et al., submitted for publication), a horizontal spray boom was used. For bay laurel, a vertical spray boom system was used (Foqué et al., in press; Nuyttens et al., 2009a). For the bay laurel crop, the first tests revealed an optimal spray volume of 4900 l ha1 ground surface corresponding with 1470 l ha1 crop surface (Foqué et al., in press; Nuyttens et al., 2009a). The extended range flat fan (XR 110 03), the Venturi flat fan (ID 120 02) and the hollow cone nozzle type (TXB 80 02), led to the highest depositions in a conically pruned bay laurel pot plant crop (Foqué et al., in press; Nuyttens et al., 2009a). The plants used for these trials were 1.00e1.20 m high and had a maximum base diameter of 0.50 m. Despite a relatively high application rate, particularly when compared to those used spraying arable crops (25e200 l ha1)(Butler Ellis and Scotford, 2003), fruits (50e500 l ha1) (Cross et al., 2001a), field-grown vegetables (150e400 l ha1) (Jensen and Nielsen, 2008; Piché et al., 2000), or ornamental liners (187e374 l ha1) (Zhu et al., 2011), this would already mean a significant reduction of the pesticides applied compared to the rates now used by bay laurel growers using their standard technique (spray gun application, up to 7000 l ha1) (Foqué et al., in press; Nuyttens et al., 2009a). Many studies describe that angling the nozzles is an efficient, easily adjustable, and inexpensive way to improve the deposition or penetration in the canopy of the crop (Combellack and Richardson, 1985; Derksen et al., 2007b, 2010; Dorr, 1990; Foqué and Nuyttens, 2011a, 2011b; Göhlich and Jegatheeswaran, 1976; Jensen and Spliid, 2005; Jensen, 2007, 2012; Lee et al., 2000; Richardson, 1987; Sayinci and Bastaban, 2011; Tunstall et al., 1965). Air support (Derksen et al., 2001; Foqué and Nuyttens, 2011a; Foqué et al., submitted for publication; Gan-Mor et al., 1996; Lee et al., 2000; Ozkan et al., 2006; Panneton et al., 2005; Panneton and Piché, 2005; Turner and Matthews, 2001; Val et al., 1996; van de Zande et al., 2002; Vandermersch et al., 2001) or a combination of both air support and an angled spray (Foqué and Nuyttens, 2011a; Foqué et al., submitted for publication; Panneton et al., 2000; Scudeler and Raetano, 2006; Womac et al., 1992) also shows promising results. Treating the crop from 2 directions has been suggested to overcome lower deposition on the back side of the plants (Derksen et al., 2007a, 2008). We therefore studied the effect of nozzle type, angled nozzles, air support and spraying in 2 passes with an opposite direction for vertical spray boom applications. The results were then compared to the standard spray boom application using the optimal settings for a bay laurel crop as suggested by our first set of experiments in bay laurel (Foqué et al., in press; Nuyttens et al., 2009a). 2. Materials and methods 2.1. Spray application techniques A self-propelled aluminum spray unit described by Foqué and Nuyttens (2010, 2011b) was equipped with 2 vertical spray booms and 2 sleeve-like air support systems, mimicking the Hardi Twin air support system (Foqué and Nuyttens, 2011a; Nuyttens et al., 2007a) (Fig. 1). Nine spray application techniques were tested to evaluate the effect of nozzle spray direction (standard (0 ), forward (30 ) or backward (30 ) relative to the movement of the spray boom), spraying with or without air support or using 2 successive sprays in an opposite direction (Table 1). Based on Foqué et al., in press, 3 nozzle types were tested, i.e. the extended range flat fan TeeJet XR 110 03 nozzle, the hollow cone TXB 80 02 nozzle and the Venturi flat fan ID 120 02 nozzle. Volume median diameters (VMD) of these nozzle-pressure combinations were determined without air assistance, at 0.50 m distance from the nozzle using a PDPA laser-based measuring setup (Nuyttens et al., 2007a, 2009c). The application technique with the standard 0 spray direction without air support and use of the extended range flat fan nozzles (XR 0 NA) is defined here as the standard technique. Based on the results of previous experiments with a horizontal spray boom system (Foqué and Nuyttens, 2011a; Foqué et al., submitted for publication; Panneton et al., 2000; Panneton and Piché, 2005), the air support was set to 30 m s1 at the air outlet. The nozzle Author's personal copy D. Foqué et al. / Crop Protection 41 (2012) 77e87 Fig. 1. Automated tunnel sprayer equipped with 2 horizontal spray booms and 2 sleeve-like air support systems: vertical spray boom, aluminum frame of the setup. spacing and boom settings were derived from previous tests (Fig. 2) and applications were made with the suggested application rate by Foqué et al., in press of 1470 l ha1 crop surface. Unlike in the preceding trial (Nuyttens et al., 2009a; Foqué et al., in press) and based on earlier findings (Braekman et al., 2009, 2010; Foqué and Nuyttens, 2011a, 2011b; Nuyttens et al., 2004), a fixed spraying distance of about 30 cm to the outermost leaves of the canopy was maintained instead of 30 cm to the main stem of the bay laurel crop (Foqué et al., in press). Because of the resulting bigger distance between rows (about 1.10 m instead of 0.60 m in Foqué et al., in press)) the application rate of 1470 l ha1 crop surface corresponds with 2600 l ha1 of ground surface. This application rate is still quite high, but already closer to the minimum application rate that is often suggested by manufacturers to treat ornamental crops in greenhouse conditions (1000 l ha1). Each spray application was repeated 3 times except for the application with a forward spray angle, made in 2 successive sprays (XR 30 NA (2)), because one repetition was made at a wrong spray pressure. 2.2. Experimental setup The experimental setup was very similar to the setup described in and Foqué et al., (in press). Nine conically pruned bay laurel pot plants (L. nobilis, 1.00e1.20 m high and maximum 0.50 m base diameter, approximately eight years old) were placed in a single 79 rails of the automated spray track, sleeve-like air assistance, row. Four of them were equipped with deposition collectors (Fig. 2). The inter-plant distance (stemestem) was 55 cm. 2.3. Spray deposition measurements To evaluate spray deposition and penetration in the canopy, 9 mineral chelate tracers (B, Ca, Co, Cu, Fe, Mg, Mn, Mo and Zn) and filter paper collectors (FPCs, Schleicher & Schuell, 5.6 cm 2.5 cm, 2589 D, Filter Service NV, Eupen, Belgium) were used. These chelates, all commercially available as horticultural leaf fertilizers (ChelalÒ; BMS Micro-Nutrients NV, Belgium), were mixed with tap water to result in a tank mix of about 1.0 g L1. Except for B, Ca and Mg, our research group had successfully used these mineral chelate tracers in previous experiments (Braekman et al., 2009, 2010; Foqué and Nuyttens, 2011a, 2011b; Foqué et al., in press, 2012, submitted for publication; Langenakens et al., 2002; Nuyttens et al., 2004, 2009a, 2009b). Furthermore, the reliability of the tracer method has been confirmed (Cayley et al., 1987; Cross et al., 2001a, 2001b, 2003; De Moor et al., 2002; Garcia-Ramos et al., 2009; Gil et al., 2007; Murray et al., 2000). The FPCs were placed on 20 positions in each of the 4 collector plants (Fig. 2). These were: the stem (S), the leaves’ upper side (L up) and the leaves’ underside (L un) in 2 crop zones (zone I (Z1) and zone II (Z2)). Because of the small size of the leaves and to prevent contamination caused by run-off, smaller sized FPCs were used for Table 1 Overview of the spray application techniques and spray parameters considered. No. of repetitions Technique 3 3 3 3 3 3 3 3 2 IDa 0 A ID 0 NA TXBb 0 A XRc 0 A XR 0 NA XR 30 A XR 30 NA XR 30 NA XR 30 NA (2) a Direction of the spray ( )d VMD (mm)e 0 0 0 0 0 30 30 30 30 e 311.6 23.1 168.3 1.6 e 179.9 6.5 e e e e Air support Spray pressure (bar) Speed (km h1) Nominal flow rate (L min1) Application rate (l ha1 ground surface) Yes No Yes Yes No Yes No No No 6.9 6.9 7.0 3.1 3.1 3.1 3.1 3.1 3.1 2.47 2.55 2.52 2.36 2.45 2.37 2.40 2.39 4.88 1.22 1.22 1.20 1.18 1.18 1.18 1.18 1.18 1.18 2664 2614 2510 2629 2640 2655 2644 2637 2571 Totalf 34 58 134 89 31 51 34 45 106 Z1g Z2h 1598 1568 1506 1578 1584 1593 1586 1582 1543 1066 1045 1004 1052 1056 1062 1058 1055 1028 ID 120 02: Venturi flat fan nozzle, Lechler GmbH, Metzingen, Germany. TXB 80 02: hollow cone nozzle, TeeJet Technologies, Wheaton, U.S. c XR 110 03: extended range flat fan nozzle, TeeJet Technologies, Wheaton, U.S. d Direction of the spray relative to the movement of the spray boom: a forward (30 ), backward (30 ) or a spray aimed directly towards the crop (0 ) was used. e Volume median diameter below which smaller droplets constitute 50% of the total volume. Three replica measurements were made for each nozzle type (mean SD). All measurements were made without air assistance. f The spray volume is presented as the mean application rate overall sprays (mean SD). g 6 out of 10 nozzles aimed to crop zone 1 (Fig. 2). h 4 out of 10 nozzles aimed to crop zone 2 (Fig. 2). b Author's personal copy 80 D. Foqué et al. / Crop Protection 41 (2012) 77e87 Fig. 2. Experimental setup and the different collector positions. the underside collector positions (3.8 cm 2.5 cm). In Z1, leaf collectors were placed in the front (F), back (B), left (L) and right (R), relative to the spray boom movement; in Z2, leaf collectors were only located on the front and back sides of the collector plants (Fig. 2). In addition to the 4 plant repetitions, 3 spray repetitions per application technique were performed (Table 1). For each set of 9 spray applications, a new set of FPCs and a different mineral chelate tracer per application technique was used. After each spray event, the exact tank concentration was determined by taking 4 tank samples (200 ml per collector) and the FPCs were allowed to dry completely before making a new application. After 9 spray applications, the FPCs were gathered and analyzed by inductively coupled plasma analysis (ICP analysis, VISTA-PRO, Varian, Palo Alto, CA, USA). The amount of tracer liquid deposited onto each collector (l ha1) was calculated. This calculation accounted for the actual concentration in the tank, the application rate, the size of the collector, the quantity of 0.16 M nitric acid (66þ%, pro analysis, Acros Organics, Geel, Belgium) used for extraction, and the results of the analysis of the blanks. 2.4. Statistical analysis In this experiment, 2160 deposition measurements were made (20 collector positions 4 collector plants 9 tracers 3 repetitions). The deposition results were also expressed as relative deposition values (%), as done in previous experiments (Braekman et al., 2010; Foqué et al., submitted for publication, in press, 2012; Nuyttens et al., 2009a). Although the differences in actual application rates are rather small (Table 1), this allowed for a more direct comparison between techniques. The datasets was examined for normality using ShapiroeWilk’s normality test but didn’t prove to have a normal distribution and (W ¼ 0.76, p < 0.001) and showed a distinct right skewness. To examine whether parametric statistical tests could be used for further analysis, the relative deposition dataset was transformed using a natural (base e) logarithm and base square root and assessed for normality as described above. To allow logarithmic transformation, the absolute depositions equal to zero were replaced by a deposition of 1.38 l ha1, which corresponds to the detection limit of the ICP-analysis (0.01 103 g L1). The square root transformed relative datasets (W ¼ 0.96, p < 0.001) showed the most promising ShapiroeWilk’s test results. A factorial ANOVA was used to examine the effect of the application technique and the collector position. The interaction term technique collector position was significant (F(171, 1816) ¼ 1.61, p < 0.001) and resulted in 62 homogenous groups shown by the post hoc test (Duncan). Because of the impracticality of discussing all of these significant differences in one paper, the deposits made to the same collector Author's personal copy D. Foqué et al. / Crop Protection 41 (2012) 77e87 position are compared to the standard XR 0 NA application technique defined as a vertical spray boom system, equipped with XR 110 03 nozzles without air support and with a standard 0 spray direction (nozzles aimed directly towards the crop). This technique also showed good results in previous tests (Foqué et al., in press; Nuyttens et al., 2009a). Significant differences are indicated by * in Figs. 6e8. Additionally, all spray application techniques with a 30 forward spray direction were compared to the XR 30 NA spraying technique. This allowed an assessment of the individual effect air support (XR 30 A) or 2 subsequent sprays with an opposite direction (XR 30 NA (2)) for the same nozzle type (XR) and spray direction (30 ). Significant differences are indicated by > in Figs. 6e8. Furthermore, the deposition results of the same application technique at different collector positions in the same plant zone were compared. Four plant zones were defined, i.e. upper side of the leaves in ZI, upper side of the leaves in ZII, lower side of the leaves in ZI and upper side of the leaves in ZII. Significantly different depositions are given a different letter label in Figs. 6e8. To evaluate the individual effect of nozzle type with and without air support, an additional analysis was made that only used the spray boom configurations with a standard 0 spray direction (Table 1). Doing so, the interaction term technique collector position was no longer significant and a main effect ANOVA could be used. Both technique (F(4, 1053) ¼ 11.82, p ¼ 0.001) and collector position (F(19, 1053) ¼ 39.37, p < 0.001) showed a significant impact on deposition. The differences between the collector positions were assumed to be the same for all techniques, therefore only the differences between techniques are discussed in this paper (Fig. 5). Again, a Duncan post hoc test was used to investigate the differences between techniques. Because a different spray distance to the crop was used in the first series of experiments (Nuyttens et al., 2009a; Foqué et al., in press), these experiments allowed an evaluation of the effect of spray boom distance to the crop for the XR 0 NA and the ID 0 NA technique as these techniques were included in both series of experiments. In contrast to the above, the square root transformed absolute dataset could be used for further parametric statistics. The transformed dataset showed a distribution close to Gaussian (W ¼ 0.96, p < 0.001). Again, a factorial ANOVA was used to examine the effect of the application technique and the collector position. The interaction term technique collector position was again significant (F(171, 1816) ¼ 2.06, p < 0.001) and the post hoc test 81 (Duncan) revealed 22 homogenous groups. Only the differences in depositions at the same collector position are discussed (Fig. 9). As both transformed datasets did not show a perfect normal distribution, the residuals of the statistical models were examined as well. Given that the residual values were all normally distributed (Fig. 3), the model had a high explanatory value. All statistical analysis were performed with Statistica 9.1 (Statsoft Inc., Tulsa, OK, USA). A p-value less than 0.05 was considered statistically significant. 3. Results and discussion 3.1. Influence of collector position The factorial ANOVA analysis showed that collector position had a significant effect. The differences between all 20 collector positions are shown in Fig. 4. This graph gives a good idea about the average depositions on the different collectors and the relations between them. In general, a significantly higher deposition was found on collectors on the front side of the plant compared with corresponding collectors on the back side of the plant (e.g. Z1 L B b vs. Z1 L F b). Since the interaction term (technique collector position) was significant as well, these significant differences between the back side and the front side were not necessarily observed with all application techniques. In Figs. 6e8 it is shown that for some techniques, the difference between front and back is no longer significant although the same trend was followed in most cases. Other techniques, however, resulted in a more homogeneous deposition on the 4 comparable collector positions as discussed below. The relative depositions in Z2, however, were generally lower than those in Z1, even though the results are expressed relative to the spray volume used (Figs 4, 6e8). This was a direct consequence of the lower spray volume applied to this zone due to a smaller number of nozzles used in the upper canopy (Table 1, Fig. 2) based on previous experiments (Nuyttens et al., 2009a; Foqué et al., in press). 3.2. Influence of spray application technique When all spray application techniques were assessed in 1 dataset, a significant interaction between technique and collector position was found. This indicates that the relationship between Fig. 3. Residuals of the statistical models used. (A) The residual values of the ANOVA used to compare the different techniques used in present study. (B) The residual values of the ANOVA used to compare the depositions made by the XR 0 NA and ID 0 NA technique techniques included in both trials. Author's personal copy 82 D. Foqué et al. / Crop Protection 41 (2012) 77e87 Fig. 4. The effect of collector position as shown by the factorial ANOVA, based on the square transformed, relative deposition dataset. Collector positions that significantly differ in deposition have a different letter label. the different collector positions was no longer the same for all techniques considered. The effect of application technique is discussed in more detail. 3.2.1. Standard application technique Except for the optimized spray boom settings (Nuyttens et al., 2009a; Foqué et al., in press), the extended range flat fan nozzle type without air support, pointed directly toward the crop (XR 0 NA) was very little different from the vertical spray boom technique generally used in practice. This technique was thus defined as the standard technique in this paper. None of the tested techniques resulted in significantly higher depositions (Figs. 6e8). Consequently, it can be accepted that this application method produced the most optimal spray deposits in this bay laurel crop considered. The standard XR 0 NA technique, however, did show some significant differences in deposition between the collector positions attached to the upper side of leaves in Z1 (Fig. 6A). Highest deposits were found in the front, followed by the right, the left and the back of Z1. The difference between front and back is caused by the direction of movement of the spray and the resulting shading effect of the crop (Derksen et al., 2007a, 2008). At the stem collectors in Z1 (Fig. 8A), deposition on the left side of the stem was significantly higher than on the right side, although a similar deposition is expected. This could indicate that the depositions on the stem in Z1 were secondary deposits caused by run-off. The less equal liquid distribution on the stem and the upper side of the leaves could result in a lower bio-efficacy when compared to techniques with a more uniform spray deposition (Barber et al., 2003; Gan-Mor et al., 1996; Nuyttens et al., 2004). Nevertheless, growers do not consider the deposition on the upper side of the leaves to be problematic (Foqué and Nuyttens, 2010, 2011b). In Z2, the deposits for the standard technique showed no significant differences between the collectors on the underside of leaves (Fig. 7A and B) or the ones attached to the stem collector (Fig. 8B). 3.2.2. Combined effect of nozzle type and air support for the standard 0 spray direction To assess the combined effect of nozzle type and air support, relative deposition values of the techniques with a standard spray direction (0 ) were evaluated separately (Fig. 5) and compared with the standard XR 0 NA technique. Overall, the highest relative deposition was found for this standard technique. Using the same standard flat fan nozzle type with air support (XR 0 A) resulted in lower deposition values. On the other hand, the use of air support did result in significantly higher mean relative depositions for the Venturi flat fan nozzle type when comparing the ID 0 A configuration with the ID 0 NA configuration. The opposite effect of air support was thus found depending on the droplet size characteristics. Together with the ID 0 NA application, the fine droplet size hollow cone application with air support (TXB 0 A) produced the significantly lowest mean depositions in the laurel crop. These results indicate that the use of air support in this horizontal crop only had a positive effect for coarse droplet sprays and no effect or even a negative effect for finer droplet sprays. This outcome is in contrast with previous horizontal spray boom experiments (Foqué and Nuyttens, 2011a; Foqué et al., submitted for publication) which demonstrated a strongly positive effect of air support in combination with the fine-to-medium extended range flat fan nozzle and a more limited positive effect in combination with a coarse droplet spray. The Venturi flat fan nozzle (ID 0 NA) resulted in a significantly lower total deposition than the extended range flat fan nozzle (XR 0 NA) (Fig. 5), although no significant differences were found for most of the individual collector positions. Only on the upwardlyoriented leaf collector positioned on the front side of the plants in Z1, the Venturi flat fan nozzles without air support (ID 0 NA) gave significantly lower deposits compared with the reference technique (Fig. 6A). Moreover, the ID 0 NA technique never resulted in significant differences between the different orientations of collectors of the same type, which was not the case for the reference techniques. Because of the more uniform spray distribution, represented by the low C.V. value in Fig. 5, caused by the higher penetration capacity of the coarse droplet spray (Braekman et al., 2010; Foqué and Nuyttens, 2011b), the ID 0 NA technique could still result in a better efficacy (Barber et al., 2003; Gan-Mor et al., 1996; Nuyttens et al., 2004). Again, this shows the importance of selecting the right nozzle type most fitted to a specific application. When the Venturi flat fan nozzle was combined with air support (ID 0 A), a higher deposition was measured on the upper side of leaves in the front of plant zone Z1, compared with the left and the back side of this zone (Fig. 6A). No other significant differences were found in the different plant zones (Figs. 6e8) indicating a relative uniform canopy spray distribution. The ID 0 A technique, Fig. 5. The means (mean SE) and coefficients of variation (C.V.) of the relative depositions of 3 nozzle types (ID: Venturi flat fan ID 120 02; TXB: hollow cone TXB 80 02 and XR: extended range flat fan TeeJet XR 110 03) used with a normal 0 orientation of the spray, with (A) or without (NA) air support. Bars with a different label are significantly different. Author's personal copy D. Foqué et al. / Crop Protection 41 (2012) 77e87 83 a horizontal boom application (Foqué and Nuyttens, 2011a; Foqué et al., submitted for publication; Panneton and Piché, 2005; Womac et al., 1992), no resistance is given to the spray past the canopy, increasing the risk of blowing the spray beyond the foliage of a single crop row. With a horizontal boom, fine to medium sprays will still result in secondary deposits due to the turbulence that occurs when the air flow hits the soil. For a vertical approach, the air support should be set in a way that the wind speed is high enough to overcome the resistance of the foliage but does not blow the spray past the canopy. When the crop is treated from 2 sides at the same time, the colliding nebulas will probably further improve the deposition in the crop. Therefore, in addition to the boom settings and nozzle type (Braekman et al., 2010), the right amount of air should be used. Based on these experiments, other than a higher homogeneity for the XR 0 A configuration and a significantly higher deposition of the ID 0 A technique when compared to the ID 0 NA application, the use of air support in a vertical crop does not seem to compensate for the high cost of this technique, as higher deposits can be achieved without air support (XR 0 NA). The TXB 0 A application, together with the ID 0 NA technique, resulted in the lowest overall mean relative depositions and the highest C.V. values in the bay laurel crop (Fig. 5). Compared with the standard technique, a significantly lower deposition was found in the dense (LAI ¼ 0.89 0.13) (Nuyttens et al., 2009a; Foqué et al., in press) crop zone (Z1) on the upper side of the leaves in the front side of the plants (Fig. 6A) and on the stem collectors at the left and back of the plants (Fig. 8A). In Z2 (LAI ¼ 0.09 0.22) (Nuyttens et al., 2009a; Foqué et al., in press) on the underside of the leaves (Fig. 7B), this technique also led to significantly lower deposits on Fig. 6. Relative deposition (mean SE) on the upper side of the leaves of the bay laurel crop in Z1 (A) and Z2 (B), respectively. * ¼ depositions which are significantly different from the XR 0 NA technique at the same collector position. > ¼ depositions which are significantly different from the XR 30 NA technique at the same collector position. a, b ¼ depositions with a different letter label are statistically different for the same technique. however, never resulted in significantly higher spray deposits than the XR 0 NA technique (Figs. 5e8). The air assisted equivalent of the reference technique (XR 0 A) gave no significant differences between collectors in the same zone (Figs. 6e8) except for on the upper side of the leaves in zone I (Fig. 6A). In this zone and in agreement with the reference technique, a lower deposition was found at the back of the plant. On the stem collectors in Z1 (Fig. 8A), the use of air support resulted in a more uniform distribution than the reference technique although none of the different collector positions showed a significantly higher deposition than for the reference technique. This technique seems to cope with the run-off problem hypothesized for the XR 0 NA technique. On the one hand, this could be explained by a more uniform spray distribution in the crop as suggested by the deposition on the stem in Z1 and the low C.V. value in Fig. 5. On the other hand, the absence of run-off could also be ascribed to a bigger portion of the spray volume that is lost due to the use of air support. This last explanation is more likely since the overall mean deposition of the XR 0 A application was found to be lower than the one of the XR 0 NA technique (Fig. 5). Differences in the effect of air support depending on the nozzle type and the corresponding droplet size spectrum (Fig. 5) reinforces the assumption that the air speed (30 m s1 at the outlet) was too high for applications with a vertical boom in this bay laurel crop. Drift experiments (Nuyttens et al., 2007a, 2010) demonstrated that ID nozzle applications (coarse droplets) are less affected by the use of air support than those made with the XR nozzle type (fine to medium droplets) because of their bigger droplet size (Braekman et al., 2010; Foqué and Nuyttens, 2011a, 2011b). In contrast to Fig. 7. Relative deposition (mean SE) on the underside of the leaves of the bay laurel crop in Z1 (A) and Z2 (B), respectively. * ¼ depositions which are significantly different from the XR 0 NA technique at the same collector position. > ¼ depositions which are significantly different from the XR 30 NA technique at the same collector position. a, b ¼ depositions with a different letter label are statistically different for the same technique. Author's personal copy 84 D. Foqué et al. / Crop Protection 41 (2012) 77e87 the back side compared to the front side of the plants. Because of the lower depositions on the stem, the underside of the leaves and the back of the plants, using a hollow cone nozzles in combination with air assistance is less suitable for applications in a bay laurel crop, as these difficult to reach locations in the plant are of special importance to the growers (Foqué and Nuyttens, 2011a, 2011b). Probably this is an effect of the air speed (30 m s1 at the outlet) being too high as well as, in the first set of trials (Nuyttens et al., 2009a; Foqué et al., in press), the non-air supported TXB did result in depositions comparable to those of the XR and ID nozzle type. This statement is supported by the conclusion of Nuyttens et al. (2007b) that air support has the highest impact on finer sprays. 3.2.3. Combined effect of spray direction and air support The effect of spray direction (30 , 0 and 30 ) was evaluated comparing the results of the XR 30 NA, the XR 0 NA and the XR 30 NA application and the XR 0 A and XR 30 A application. No significant differences were found between the XR 30 NA and the XR 0 NA reference technique (Figs. 6e8). However, similar to the standard technique, the XR 30 NA application resulted in the same significant differences between collector positions on the upper sides of the leaves in Z1 (Fig. 6A). Also on the stem in Z1 (Fig. 8A) a higher deposition on the left side was found compared with the back and right sides, probably due to run-off. Additionally, the stem deposition in Z1 was significantly higher in the front compared with the back because of the shading effect. When a forward spray angle was used without air support, this configuration seemed to intensify the shading effect created by the canopy, creating differences between the front and the back of the plants. Additionally, significant differences in upper side leaf deposition between the front and back in Z2 were observed for the XR 30 NA as well as for the XR 30 A technique (Fig. 6B). Moreover, in Z2 at the underside of the leaves (Fig. 7B), higher depositions were found in the front compared to the back of the plants for the XR 30 A technique. This confirms that a 30 spray direction increased the shading effect and the difference in deposition between front and back. These findings are in agreement with Nuyttens et al. (2009a) and Foqué et al., in press) who found that the difference between the deposition on the front side and the back side of the plant was most pronounced for the deflector flat fan nozzle because of its forward spray direction. The use of air support only seems to intensify these differences. Compared to the reference technique, the use of a backward spray direction (XR 30 NA) resulted in significantly lower depositions on the upper side of the leaves in Z1 (Fig. 6A) and on some stem collectors in Z1 and Z2 (Fig. 8). In particular, the lower deposition values at the front of the plants were characteristic for a 30 spray direction. In contrast to the reference technique, no significant differences were found between alike collector positions in the different plant zones (Figs. 6e8). Therefore, use of a backward spray direction did not result in a higher total spray deposition compared with the reference technique but it did result in a more uniform spray distribution, which is also an important factor with regard to a good biological efficacy (Barber et al., 2003; Gan-Mor et al., 1996; Nuyttens et al., 2004). Womac et al. (1992) found that orienting the air stream of an air assisted sprayer 30 backwards increased deposits on the underside of leaves. In Braekman et al. (2010), however, an air-assisted spout fitted with an ISO 80015 flat fan nozzle type oriented 45 upwards and 30 backwards resulted in the lowest deposits. Because the other boom setting did result in better depositions, the authors concluded that the air-assisted spouts could lead to even better deposition results when appropriate setting is used. This suggests that using a backward spray angle in combination with air support could result in further improvements. 3.2.4. Effect of the number of passes with a 30 spray direction The effect of an application in 2 runs with an opposing direction was evaluated for the spray boom setup with extended range flat fan nozzles and a 30 spray direction by comparing the XR 30 NA (2) and the XR 30 NA techniques. The 2-run application (XR 30 NA (2)) never showed significantly different depositions for collectors attached in the same plant zone (Figs. 5e7). This result agrees with Derksen et al. (2007a, 2008), who concluded that treating the crop in 2 directions is a good way to overcome a lower deposition on the back side of the plants. However, on Z1 S L (Fig. 8A) and Z1 L F up (Fig. 6A), this technique booked significantly lower deposits than the standard technique. As the XR 30 NA (2) application never resulted in significantly higher depositions than the standard technique, no statistical evidence was found for a better performance of spraying in 2 runs with an opposite direction. However, the more uniform spray distribution could result in an improvement (Barber et al., 2003; Gan-Mor et al., 1996; Nuyttens et al., 2004). Fig. 8. Relative deposition (mean SE) made to the stem in the lower (A) and upper region (B) of the bay laurel crop. * ¼ depositions which are significantly different from the XR 0 NA technique at the same collector position. > ¼ depositions which are significantly different from the XR 30 NA technique at the same collector position. a, b ¼ depositions with a different letter label are statistically different for the same technique. 3.2.5. Effect of air support with a 30 spray direction The effect of air support for a 30 spray direction was evaluated by comparing the XR 30 A technique with the reference technique and the XR 30 NA technique. In general, the XR 30 A technique led to deposits comparable to the ones of the XR 30 NA and XR 0 NA applications (Figs. 6e8) except at the collector positions Z1 L B up and Z2 L B up (Fig. 6) and Z1 L F un (Fig. 7A) where lower depositions were found compared Author's personal copy D. Foqué et al. / Crop Protection 41 (2012) 77e87 with the reference technique. Again, this might indicate the effect of spray loss because of the use of air support. Moreover, in contrast with the techniques without air support (XR 0 NA and XR 30 NA), the XR 30 A applications resulted in a less uniform spray distribution in the crop. In contrast to the results of our laboratory tests with a horizontal spray boom (Foqué and Nuyttens, 2011a; Foqué et al., submitted for publication), air support did not have a positive effect on the spray results in combination with an extended range flat fan nozzle and a forward spray angle when a vertical boom was used. 3.3. Effect of spray distance Fig. 9 presents the results of the ID Venturi flat fan nozzle (without air support and a standard 0 spray direction) and the reference technique at two spray distances as both techniques were tested for a spray distance of 30 cm to the stem (Nuyttens et al., 2009a; Foqué et al., in press) as well as for a spray distance of 30 cm to the outermost leaves of the canopy (Braekman et al., 2009, 2010; Foqué and Nuyttens, 2011a, 2011b; Nuyttens et al., 2004). For the ID nozzle, significantly higher depositions were booked for collector positions Z1 L R un, Z1 L F un, Z1 S B, Z1 S L, Z1 S R, Z1 S F and Z2 L B un for the 30 cm spray distance to the stem (Fig. 9, ID 120 02 (1)) compared with the 30 cm spray distance to the leaves (Fig. 9, ID 120 02 (2)). For the XR nozzle type, the 30 cm spray distance to the leaves only resulted in significantly higher depositions on collector position Z1 L F up and in significantly lower depositions at collector positions Z1 L R un, Z1 S R and Z1 S F. On most collector positions, however, no statistically significant effect of spray distance on spray deposition for the XR nozzle type was observed. These results show that a spray distance of 30 cm to the stem proved to be more effective when spraying conically shaped plants. Therefore, the fixed spray distance of a vertical spray boom to the crop should be determined based on the smallest zone of the canopy. This allows for cultivation of more plants on the same area without jeopardizing good plant protection. For this bay laurel crop (Ø 50 cm), some nozzles only passed at a distance of 5 cm from the outermost edges of the canopy. This Fig. 9. Results of the factorial ANOVA, based on the square transformed, absolute deposition dataset of both bay laurel trials with a spray distance of 30 cm to the stem (1st trial) and a spray distance of 30 cm to the outer crop zone (2nd trial). A ¼ significant differences in deposition of the extended flat fan nozzle type between the first (XR 110 03 (1)) and the second trial (XR 110 03 (2)), at the same collector position. - ¼ significant differences in deposition of the Venturi flat fan nozzle type between the first (ID 120 02 (1)) and the second trials (ID 120 02 (2)), at the same collector position. * ¼ depositions that are significantly higher than all the other unmarked depositions at the same collector position. 85 spray distance seems inadequate for good spray coverage. Literature (Knewitz et al., 2003; Stallinga et al., 2004), however, shows that using a smaller nozzle spacing enables a reduction in spray distance. Consequently, the smaller nozzle spacing in Z1 allowed us to reduce the spray distance. For nozzles with a 110 spray angle, a nozzle spacing of 12.5 cm results in a theoretically uniform distribution for such a small spray distance. Smaller spray angles will result in a more banded spray. Stallinga et al. (2004) used a smaller nozzle size to keep the spray volume constant when reducing nozzle spacing. In our trials, however, the same nozzle type was fitted to all nozzle caps. The higher spray volume used in Z1 was justified based on the high LAI in this zone, which was 10 times higher than that used in Z2 (Nuyttens et al., 2009a). These findings led us to the following hypothesis: as proposed in literature (Braekman et al., 2009, 2010; Foqué and Nuyttens, 2011a, 2011b; Nuyttens et al., 2004), a vertical boom with a nozzle spacing of 37.5 cm can be used at a fixed spray distance of about 30 cm to the outer edges of the canopy for hedge-like crops or plants with a regular shape (e.g. columnar, cubical or block-shaped). When spraying plants with a more irregular pruning (e.g. pyramidal, conical, bonsai or spiral) with a vertical boom, the distance of the spray boom should be determined relative to the narrowest area in the shape. A smaller nozzle spacing should be used to provide a good coverage on places where the canopy gets closer to the nozzles. In both cases, water-sensitive paper can be used to select the best combination of nozzle size and settings (Braekman et al., 2009; Nuyttens et al., 2009a). In conclusion, from the wide range of tested vertical spray boom techniques tested, applications with an extended range standard flat fan nozzle without air support, directed straight toward the crop generally provided the best spray results in a bay laurel crop with a fixed spray distance to the stem of about 30 cm. A closer nozzle spacing is used as it provides the best coverage on leaves closest to the nozzles. This technique is considered here as the reference technique. The Venturi flat fan nozzle provided a valuable alternative for the extended-range flat fan nozzles. When using the appropriate spray distance to the crop, this nozzle type often resulted in the highest deposits and one of the most uniform spray distributions. The use of an adapted spray direction only proved to add value using a backward spray direction (30 ) or when an application with a forward spray direction (30 ) was applied in 2 opposing successive runs. Although no higher deposits were found than the ones of the reference technique, both spray applications improved spray uniformity in the canopy. The use of air support only resulted in a significant rise in the overall deposition values for the Venturi flat fan nozzle and a more uniform spray distribution for the extended range flat fan and the Venturi flat fan nozzle with the standard spray direction. In combination with a forward spray direction, the use of air support increased the differences in deposition between the front and the back of the plants. In general, air support did not have a positive effect on the spray deposition values or the penetration of the spray in the canopy. To improve the effect of air support, the air speed and volume as well as spray direction should be adapted based on the crop and spray characteristics. Acknowledgments The authors would like to thank the Flemish Government (IWT Vlaanderen) for financial support. 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