SRM UNIVERSITY
DEPARTMENT OF BIOTECHNOLOGY
SCHOOL OF BIOENGINEERING
SUBJECT: ENZYME TECHNOLOGY
SEM: III SEM B.TECH
SUBJECT CODE: BT0201
Year: 2012
LECTURE SCHEDULE
Lecture
Topic / Content
Hours
Book
Learning outcomes
UNIT I
CLASSIFICATION AND APPLICATION OF ENZYMES
1
Introduction to enzymes
1&2
2
Classification of enzymes
1,2,5
3
Types of enzyme – Constitutive and Induced type Intracellular and extracellular enzyme
1&2
4
Specificity of enzyme action
1,2,5
5
Factors modifying enzyme activity
1&2
6
Biotechnological applications of enzymes
7
Applications of enzymes in other industries
8
Analytical applications of enzymes
9
Applications of enzymes in disease treatment and
1&7
1
diagnosis
10
1
Applications of enzymes in diagnosis
Students will understand
the types of enzymes,
factors affecting enzyme
actions and its applications
in the industries and their
great importance in
scientific research, clinical
diagnosis and industry.
1
1
UNIT II
Chemical nature of enzyme catalysts
11
Structural components of enzymes,
1
12
Concept of active site and energetic of an enzyme
1
13
Cofactor, apoenzymes, prosthetic groups
1
To learn about the structure
14
Active sites, Lock and Key hypothesis
1
of enzymes, apoenzymes,
15
Koshland Induced fit hypothesis
1,6
prosthetic group, cofactors.
16
Michealis – Menten single substrate catalyzed
reactions
1,5,6
17
Problems
5,6,7
18
Line weaver’s plot and Eddie Hoftsee plot
1,6
19
Metal activated enzymes
1,6
20
Involvement of co enzymes
1,6
To study about the
mechanisms of enzymes
and its activities on metals.
UNIT III
Free and Immobilized enzyme kinetics
21
Kinetics of single substrate reactions
1
To study about the kinetics
22
Allosteric enzymes-Monad-Changeux, Wyman
Model and KNF Model
23
Substrate-enzyme complex formation and specificity
of enzyme action
1,5&6
4,5&6
24
Kinetics of multi substrate reactions
6
25
Turnover number, estimate of kinetic parameters
26
Multi substrate reactions
27
Temperature and pH effects of enzyme activity
28
Methods of Immobilization
7
29
Kinetics of immobilized enzymes
6
30
Effects of external mass transfer and intra-particle
substrate reactions.
To learn about the
immobilization methods and
its kinetics.
1,6
1
diffusion
31
of single and multiple
Deactivation kinetics
3&5,7
7
6
UNIT IV
Extraction and purification of enzymes
32
Methods of production of enzymes
1,4,7,
33
Extraction of enzymes
1,6,4
34
Extraction of membrane bound enzymes
35
Purification methods of enzymes
36
Cell disruption methods
37
Chromatography methods
4
38
Nature of extraction medium
4
39
Criteria for purity
4
40
Determination of molecular weight of enzymes
4
1,7
5
4,6
To study about the
production methods of
enzymes and its purification
techniques.
UNIT V
Instrumental Techniques in Enzymatic analysis
41
Instrumental methods of analysis- Manometry
1
42
Spectrophotometry
1
43
Spectrofluorimetry
1
44
Electrochemical methods
1
45
Enthalpimetry
1
46
Radiochemical methods
1
47
Automation in enzymatic analysis- Fixed time
methods
48
Methods involving continuous monitoring
1
1
To understand about the
principles of enzymatic
analysis.
To learn about the
instrumental methods in
enzymatic analysis.
Text Books and References
1
Enzymes
Trevor Palmer
2
Biochemistry
Leningher
3
Enzymes
Robert A. Copeland, 2nd Edition
4
5
6
7
Fundamentals of
Enzymology
Biochemical engineering
Bioprocess Engineering
Principles
Bioprocess Engineering
Nicholas C.Price
Harvey W. Blanch, Douglas S. Clark
Pauline M. Doran
Shuler and Kargi
Dr.M.Pandimadevi
(Subject Coordinator)
Dr.P.Velusamy
Mr.Y.Ravichandran