E N V I R O N M E N T P R O T E C T I O N A U T H O R I T Y Community Estuarine Monitoring Manual Community Estuarine Monitoring Manual Community Estuarine Monitoring Manual ThisȱpublicationȱwasȱfundedȱbyȱtheȱSouthȱAustralianȱEnvironmentȱProtectionȱAuthorityȱ(EPA)ȱasȱaȱ technicalȱresourceȱforȱWaterwatchȱandȱotherȱcommunityȱwaterȱmonitoringȱgroupsȱ Writtenȱandȱillustratedȱbyȱ PeriȱColemanȱandȱFaithȱCookȱ TheȱNationalȱLibraryȱofȱAustraliaȱ CataloguingȬinȬPublicationȱentry:ȱ Coleman,ȱPeriȱ Communityȱestuarineȱmonitoringȱguidanceȱmanualȱ Bibliographyȱ ISBNȱ1ȱ921125ȱ29ȱ2ȱ Estuarineȱhealth—SouthȱAustralia—Measurement—Handbooks,ȱmanuals,ȱetc.ȱȱ2.ȱWaterȱquality—ȱ SouthȱAustralia—Measurement—Handbooks,ȱmanuals,ȱetc.ȱI.ȱCook,ȱFaith,ȱ1981–.ȱII.ȱWaterwatchȱ SouthȱAustraliaȱ(Program).ȱIII.ȱTitle.ȱ ©ȱEnvironmentȱProtectionȱAuthorityȱ2007ȱ Thisȱbookȱisȱcopyright.ȱCopyrightȱtoȱallȱtextȱisȱvestedȱtoȱtheȱCrown,ȱbyȱtheȱauthorsȱandȱpublishers.ȱAȱ rightȱofȱunlimitedȱuseȱofȱallȱillustrationsȱisȱprovidedȱtoȱtheȱCrownȱbyȱtheȱartists,ȱwhileȱretainingȱ copyright.ȱApartȱfromȱanyȱfairȱdealingȱforȱtheȱpurposesȱofȱprivateȱstudy,ȱresearch,ȱcriticismȱorȱreview,ȱ asȱpermittedȱunderȱtheȱCopyrightȱAct,ȱnoȱpartȱmayȱbeȱreproducedȱbyȱanyȱprocessȱwithoutȱwrittenȱ permission.ȱEnquiriesȱshouldȱbeȱmadeȱtoȱtheȱEnvironmentȱProtectionȱAuthorityȱofȱSouthȱAustralia.ȱ PublishedȱinȱAustraliaȱbyȱtheȱEnvironmentȱProtectionȱAuthorityȱwithȱassistanceȱfromȱDeltaȱ EnvironmentalȱConsulting.ȱ ȱ ȱ Printedȱonȱrecycledȱpaperȱ Acknowledgements TheȱSouthȱAustralianȱEnvironmentȱProtectionȱAuthorityȱ(EPA)ȱ hasȱfundedȱtheȱdevelopmentȱofȱthisȱresource.ȱȱ PeriȱColemanȱandȱFaithȱCookȱ(DeltaȱEnvironmentalȱ Consulting)ȱcompiledȱtheȱguidanceȱmanualȱinȱconsultationȱ withȱstaffȱofȱWaterwatchȱSA,ȱinȱparticularȱLindaȬMarieȱHall.ȱ Theȱfollowingȱcommunityȱgroupsȱwereȱinvolvedȱinȱtheȱ communityȱtrialsȱofȱsomeȱofȱtheȱcoreȱactivities:ȱPortȱNoarlungaȱ PrimaryȱSchoolȱWaterwatchȱgroup,ȱSouthernȱDistrictsȱ Waterwatchȱgroup,ȱNoarlungaȱPrimaryȱSchool,ȱMiddleȱBeachȱ SamphireȱDiscoveryȱTrail,ȱStȱKildaȱMangroveȱInterpretiveȱ Centre,ȱtheȱMinoriesȱWaterwatchȱgroup,ȱLeFevreȱPrimaryȱ SchoolȱandȱVitasȱKarnaitis.ȱThanksȱforȱyourȱenthusiasm,ȱinȱ coldȱwetȱweather,ȱeveryone!ȱ TheȱdocumentȱwasȱreviewedȱbyȱDrȱJohnȱCugleyȱofȱtheȱEPA,ȱ andȱWaterwatchȱstaffȱSheraleeȱCox,ȱNickȱFewsterȱandȱChrisȱ Burgess.ȱȱ DrȱSueȱMurrayȬJonesȱofȱtheȱCoastȱandȱMarineȱBranchȱofȱtheȱ DepartmentȱforȱEnvironmentȱandȱHeritageȱeditedȱtheȱdraftȱ guidelines.ȱHerȱattentionȱtoȱdetailȱisȱappreciated,ȱandȱanyȱ remainingȱmistakesȱareȱtheȱentireȱresponsibilityȱofȱtheȱauthors.ȱ Thisȱmanualȱwouldȱbeȱlightȱinȱcontentȱindeed,ȱwithoutȱtheȱ workȱofȱconcurrentȱprojectsȱsuchȱasȱtheȱlocalȱestuaryȱ identificationȱresourcesȱbeingȱdevelopedȱbyȱChrisȱBallȱfromȱ ReefȱWatchȱSA.ȱSimilarly,ȱtheȱfreelyȱavailableȱresourcesȱ providedȱbyȱgovernmentȱagenciesȱinȱbothȱtheȱstateȱ(Coastalȱ AtlasȱofȱSA)ȱandȱfederalȱ(NationalȱPollutantȱInventory)ȱlevelsȱ fleshȱoutȱtheȱbonesȱprovidedȱinȱtheseȱguidelines.ȱ ȱ ȱ Table of Contents Introduction .........................................1 Lookingȱatȱtheȱvegetationȱandȱhabitat ......... 73 Meetȱtheȱmonitoringȱframework.................... 2 Keepingȱaȱvisualȱrecordȱofȱplanktonȱblooms.....74 Understandingȱestuaries ............................. 5 Gettingȱtoȱknowȱphytoplanktonȱwithȱaȱnetȱtow .75 Estuaries—valuesȱandȱimpacts...................... 7 Countingȱplanktonȱinȱaȱwaterȱsample ............78 Workingȱinȱtheȱfield—safetyȱ&ȱenvironment ..... 9 Monitoringȱaȱseaweedȱbed .........................82 Workingȱinȱtheȱfield—permitsȱ&ȱpoliteness......11 MonitoringȱanȱintertidalȱSAMȱbed ................86 Designingȱyourȱmonitoringȱprogram .............12 MonitoringȱsubmergedȱSAMȱbeds ................88 Selectingȱyourȱmonitoringȱsites ....................16 Howȱcontinuousȱisȱtheȱfringingȱvegetation? .....90 Samplingȱsiteȱobservations...................... 17 Monitoringȱmangroveȱleaf/twigȱlitterȱȱȱȱȱȱȱ production ............................................92 Recordingȱweatherȱdetails ..........................18 Recordingȱtimeȱandȱtideȱheight ....................19 Recordingȱodoursȱandȱoddȱthings .................20 Recordingȱwaterȱdepth..............................21 Recordingȱsurfaceȱwaterȱconditions ...............22 Monitoringȱmangroveȱexpansion..................94 Studyingȱsaltmarshȱplants ..........................95 MonitoringȱMelaleucaȱleaf/twigȱlitterȱȱȱȱȱȱȱȱȱȱ production ............................................98 Recordingȱwaterȱcolour .............................23 Usingȱaerialȱphotographsȱtoȱmapȱhabitatȱȱȱȱȱȱȱȱ change ............................................... 100 Measuringȱflowȱrate .................................24 Measuringȱbeachȱlitter ............................ 103 Understandingȱestuarineȱprocesses............ 25 Observingȱanimalȱlife .......................... 105 Measuringȱtheȱwaterȱtemperature .................26 Tidalȱflats—lookingȱforȱbenthicȱinvertebrates . 106 MeasuringȱpH ........................................28 Tidalȱflats—countingȱcrabȱburrows ............. 109 Measuringȱwaterȱclarity.............................30 Tidalȱflats—countingȱsurfaceȱsnails ............. 111 Measuringȱsalinity ...................................32 Tidalȱflats—recordingȱwadingȱbirds ............ 113 Mappingȱyourȱestuary ..............................36 Rockyȱshore—countingȱsnails.................... 115 Isȱyourȱestuaryȱpositiveȱorȱnegative?..............38 Saltmarsh—lookingȱforȱinvertebrates ........... 118 Classifyingȱyourȱestuary ............................40 Saltmarsh—countingȱcrabsȱandȱfish............. 121 Mappingȱtheȱsalinityȱgradientȱofȱyourȱestuary ..41 Acrossȱtheȱestuary—recordingȱraptors ......... 123 Mappingȱtheȱsalinityȱprofileȱofȱyourȱestuary.....42 Mappingȱturbidityȱplumes .........................44 Acrossȱtheȱestuary—recordingȱinvasiveȱȱȱȱȱȱȱȱȱȱ species ............................................... 125 Whereȱisȱtheȱ‘turbidityȱmaximum’? ...............46 Acrossȱtheȱestuary—monitoringȱbacteria....... 127 Effectȱofȱtidalȱrestrictionsȱonȱwetlands ............48 References ....................................... 130 Chemicalȱassessments ........................... 55 Appendixȱ1—MonitoringȱFramework .......... 132 Measuringȱdissolvedȱoxygen .......................56 Appendixȱ2—BeaufortȱScale ..................... 139 Measuringȱsulphide .................................58 Appendixȱ3—Waterȱcolourȱchart ................ 140 Measuringȱorthophosphate.........................59 Appendixȱ4—Planktonȱidentificationȱflowȱȱȱȱȱȱȱȱȱȱȱȱȱ chart ................................................ 141 Measuringȱammonium ..............................61 MeasuringȱchlorophyllȬa............................63 Measuringȱalkalinity ................................65 Measuringȱtotalȱsuspendedȱsolids .................67 Massȱbalancesȱtoȱestimateȱpollutants..............69 MeasuringȱsoilȱsalinityȱandȱsoilȱpH ...............71 ȱ Appendixȱ5—Forms ............................... 142 Appendixȱ6—Equipment ......................... 158 Glossary.......................................... 169 Eȱ S T U A R I N E MONITORING GUIDANCE MANUAL Introduction ȱ ȱ ȱ 1 ESTUARINE MONITORING GUIDANCE MANUAL Meet the monitoring framework WaterwatchȱAustraliaȱisȱaȱnationalȱcommunityȱwaterȬmonitoringȱprogramȱthatȱencouragesȱallȱ Australiansȱtoȱbecomeȱinvolvedȱwith,ȱandȱactiveȱin,ȱtheȱprotectionȱandȱmanagementȱofȱtheirȱwaterwaysȱ andȱcatchments.ȱȱ Previously,ȱWaterwatchȱconcentratedȱonȱfreshwaterȱmonitoring,ȱbutȱrecentlyȱaȱnumberȱofȱcommunityȱ groupsȱinȱestuarineȱareasȱhaveȱbecomeȱinterestedȱinȱmonitoringȱtheirȱestuaries.ȱTheȱinterestȱstemsȱfromȱ theȱmaximȱthatȱ‘estuariesȱareȱtheȱreportȱcardȱofȱtheȱcatchment’ȱ(NationalȱLandȱandȱWaterȱAudit,ȱ2002).ȱȱ Inȱresponseȱtoȱthisȱinterest,ȱtheȱEPAȱhasȱdevelopedȱanȱestuarineȱmonitoringȱframeworkȱthatȱisȱsuitableȱ forȱuseȱbyȱaȱwideȱrangeȱofȱcommunityȱgroups.ȱTheȱmonitoringȱframeworkȱ(Appendixȱ1)ȱpresentsȱaȱ rangeȱofȱactivitiesȱthatȱcommunityȱgroupsȱmayȱwishȱtoȱexplore.ȱTheȱactivitiesȱareȱdesignedȱwithȱtheȱ goalsȱof:ȱȱ – helpingȱtheȱcommunityȱunderstandȱtheirȱestuaryȱ – providingȱsimpleȱyetȱreliableȱdataȱaboutȱtheȱconditionȱofȱtheȱestuary.ȱ Theȱactivitiesȱareȱbrokenȱintoȱcoreȱandȱextensionȱactivities.ȱCoreȱactivitiesȱmayȱbe:ȱ – conductedȱbyȱtheȱwidestȱrangeȱofȱpeopleȱȱ – inȱtheȱwidestȱrangeȱofȱestuariesȱ – withȱbasicȱequipment.ȱȱ Theȱcoreȱactivitiesȱshouldȱresultȱinȱaȱgoodȱlevelȱofȱunderstandingȱofȱestuarineȱprocessesȱwhileȱyieldingȱ reliableȱdataȱonȱcommonȱestuarineȱparameters.ȱȱ Extensionȱactivitiesȱareȱdesignedȱtoȱbeȱundertaken:ȱ – byȱolderȱgroupsȱȱ – byȱgroupsȱwithȱaȱspecialȱinterestȱinȱcertainȱareasȱ(forȱexampleȱtheȱrestorationȱofȱMuttonȱCove)ȱȱ – whereȱthereȱareȱcertainȱtypesȱofȱhabitatȱȱ – whenȱparametersȱrequiringȱprocessingȱbackȱinȱaȱlaboratoryȱareȱbeingȱexamined.ȱȱ Theȱextensionȱactivitiesȱshouldȱprovideȱaȱdeeperȱunderstandingȱofȱestuarineȱprocessesȱandȱyieldȱaȱwiderȱ rangeȱofȱinformationȱaboutȱtheȱfunctioningȱofȱtheȱspecificȱestuaryȱbeingȱstudied.ȱ Theȱfollowingȱtwoȱlistsȱsummariseȱtheȱcoreȱandȱextensionȱactivitiesȱsuggestedȱinȱthisȱestuarineȱ monitoringȱframework.ȱ Core activities Samp lingȱs it eȱ obs erv at io n s ȱ – recordingȱweatherȱdetailsȱ – readingȱtimeȱandȱtideȱheightȱ – recordingȱodoursȱandȱoddȱthingsȱ – recordingȱwaterȱdepthȱ – recordingȱsurfaceȱwaterȱconditionsȱ – recordingȱwaterȱcolour.ȱ Underst anding ȱestuarine ȱp r oc e ss es ȱ – measuringȱwaterȱtemperatureȱ – measuringȱpHȱ – measuringȱwaterȱclarityȱ – measuringȱsalinity.ȱ 2 ESTUARINE MONITORING GUIDANCE MANUAL C h e m ic a lȱ ass e ssm ent s ȱ – measuringȱdissolvedȱoxygenȱ – measuringȱorthophosphateȱ – measuringȱammoniumȱ – measuringȱalkalinity.ȱ Looking ȱ atȱth eȱveg et at ion ȱandȱh ab i t a t ȱ – keepingȱaȱvisualȱrecordȱofȱplanktonȱbloomsȱ – measuringȱbeachȱlitter.ȱ O b se rv ingȱ an imal ȱ life ȱ – tidalȱflats—countingȱcrabȱburrowsȱ – tidalȱflats—countingȱsurfaceȱsnailsȱ – tidalȱflats—recordingȱwadingȱbirdsȱ – acrossȱtheȱestuary—recordingȱraptorsȱ – acrossȱtheȱestuary—recordingȱinvasiveȱspecies.ȱ E x ten sio n a c t ivi t ies Samp lingȱs it eȱ obs erv at io n s ȱ – measuringȱflowȱrate.ȱ Underst anding ȱestuarine ȱp r oc e ss es ȱ – mappingȱyourȱestuaryȱ – isȱyourȱestuaryȱpositiveȱorȱnegative?ȱ – classifyingȱyourȱestuaryȱ – mappingȱtheȱsalinityȱgradientȱofȱyourȱestuaryȱ – mappingȱtheȱsalinityȱprofileȱofȱyourȱestuaryȱ – mappingȱturbidityȱplumesȱ – whereȱisȱtheȱ‘turbidityȱmaximum’ȱforȱyourȱestuary?ȱ – effectȱofȱtidalȱrestrictionsȱonȱwetlands.ȱ C h e m ic a lȱ ass e ssm ent s ȱ – measuringȱsulphideȱ – measuringȱchlorophyllȬaȱ – measuringȱtotalȱsuspendedȱsolidsȱ – estimatingȱpollutantȱloadsȱwithȱmassȱbalancesȱ – measuringȱsoilȱsalinityȱandȱsoilȱpH.ȱ Looking ȱ atȱth eȱveg et at ion ȱandȱh ab i t a t ȱ – gettingȱtoȱknowȱphytoplanktonȱwithȱaȱnetȱtowȱ – countingȱphytoplanktonȱinȱaȱwaterȱsampleȱ – monitoringȱaȱseaweedȱbedȱ – monitoringȱanȱintertidalȱSAMȱbedȱ – monitoringȱsubmergedȱSAMȱbedsȱ – howȱcontinuousȱisȱtheȱfringingȱvegetation?ȱ – monitoringȱmangroveȱleaf/twigȱlitterȱproductionȱ – monitoringȱmangroveȱexpansionȱ 3 ESTUARINE MONITORING GUIDANCE MANUAL – studyingȱsaltmarshȱplantsȱ – monitoringȱMelaleucaȱleaf/twigȱlitterȱproductionȱ – usingȱaerialȱphotographsȱtoȱmapȱhabitatȱchange.ȱ O b se rv ingȱ an imal ȱ life ȱ – tidalȱflats—lookingȱforȱbenthicȱinvertebratesȱ – rockyȱshore—countingȱgrazingȱsnailsȱ – saltmarsh—lookingȱforȱinvertebratesȱinȱsaltmarshȱhabitatsȱ – saltmarsh—countingȱcrabsȱandȱfishȱinȱsaltmarshȱcreeksȱ – acrossȱtheȱestuary—monitoringȱbacteria.ȱ Theȱframeworkȱrecognisesȱthatȱdifferentȱgroupsȱwillȱwantȱtoȱdoȱdifferentȱthingsȱinȱtheirȱestuary.ȱSomeȱ existingȱWaterwatchȱgroupsȱwillȱbeȱcomfortableȱwithȱtheȱrangeȱofȱactivitiesȱthatȱtheyȱcurrentlyȱ undertake,ȱandȱmayȱonlyȱwishȱtoȱuseȱequipmentȱandȱmethodsȱthatȱworkȱoverȱaȱwiderȱrangeȱofȱsalinitiesȱ thanȱcurrentlyȱcateredȱforȱinȱWaterwatchȱkits.ȱ Otherȱgroupsȱmayȱbeȱattemptingȱtoȱunderstandȱorȱtrackȱdownȱaȱpollutionȱissueȱinȱtheirȱestuary,ȱwhileȱ stillȱothersȱmayȱbeȱengagedȱinȱrestorationȱworkȱinȱestuarineȱwetlandsȱandȱmayȱneedȱguidanceȱonȱhowȱtoȱ monitorȱtheȱprogressȱofȱtheȱrestorationȱwork.ȱ Theȱnextȱsectionȱofȱtheseȱguidelinesȱdiscussesȱhowȱyourȱgroupȱcouldȱselectȱactivitiesȱfromȱtheȱcoreȱandȱ extensionȱareasȱtoȱbuildȱupȱaȱspecificȱmonitoringȱprogramȱthatȱmeetsȱyourȱgroups’ȱneeds.ȱ Definitions Theȱactivityȱframeworkȱhasȱaȱnumberȱofȱcodes,ȱabbreviationsȱandȱsymbols:ȱ Ageȱgroups:ȱȱ Theȱageȱgroupsȱshownȱinȱtheȱframeworkȱareȱtheȱminimumȱrecommendedȱagesȱforȱeachȱactivity.ȱTheȱ recommendationȱmayȱbeȱbasedȱonȱskillȱissuesȱorȱsafety.ȱȱ Timeȱrequirements:ȱ 1ȱ=ȱlessȱthanȱ10ȱminutesȱ 2ȱ=ȱ10ȱminutesȱtoȱ1ȱhourȱ 3ȱ=ȱ1–2ȱhoursȱ 4ȱ=ȱmoreȱthanȱ2ȱhoursȱ Activityȱsymbols:ȱȱ ȱ ȱ=ȱthisȱactivityȱisȱaȱdesk–basedȱactivityȱȱ 3ȱ=ȱthisȱactivityȱcontainsȱsomeȱfieldȱactivitiesȱ Inȱaddition,ȱthereȱisȱaȱglossaryȱatȱtheȱendȱofȱtheseȱguidelines,ȱtoȱprovideȱdefinitionsȱforȱtechnicalȱtermsȱ usedȱinȱtheȱframeworkȱandȱguidelines.ȱ ȱ ȱ ȱ 4 ESTUARINE MONITORING GUIDANCE MANUAL Understanding estuaries Estuariesȱareȱwhereȱfreshwaterȱcreeks,ȱstreamsȱandȱriversȱmeetȱtheȱocean.ȱTheyȱmayȱbeȱhugeȱ(likeȱtheȱ Coorong)ȱorȱtinyȱsuchȱasȱtheȱsmallȱtidalȱcreeksȱenteringȱtheȱtwoȱgulfs,ȱGulfȱStȱVincentȱandȱSpencerȱGulf.ȱ Whatȱhappensȱinȱanȱestuaryȱdependsȱonȱtheȱinteractionsȱbetweenȱtheȱamountsȱofȱfreshwaterȱflowingȱinȱ (viaȱrainfall,ȱrunoff,ȱseepageȱandȱeffluentȱoutfalls),ȱtheȱamountȱofȱtidalȱexchangeȱthatȱoccursȱeachȱday,ȱ theȱrateȱofȱevaporationȱandȱtheȱsizeȱandȱfrequencyȱofȱwaves.ȱ Theȱfollowingȱdefinitionȱofȱanȱestuaryȱisȱusedȱinȱthisȱmanual:ȱȱ Anȱestuaryȱisȱaȱnarrow,ȱsemiȬenclosedȱcoastalȱbodyȱofȱwater,ȱwhichȱhasȱaȱfreeȱconnectionȱwithȱtheȱ openȱseaȱatȱleastȱintermittentlyȱandȱwithinȱwhichȱtheȱsalinityȱofȱtheȱwaterȱisȱmeasurablyȱdifferentȱ fromȱtheȱsalinityȱinȱtheȱopenȱocean.ȱ ThisȱisȱaȱwiderȱdefinitionȱthanȱthatȱofȱCameronȱandȱPritchardȱ(1963),ȱwhichȱassumedȱthatȱallȱestuariesȱ containedȱaȱmeasurableȱdilutionȱofȱseawater.ȱSouthȱAustralianȱestuariesȱhaveȱveryȱlowȱflowsȱofȱ freshwater,ȱandȱareȱthereforeȱfrequentlyȱhypersalineȱ(moreȱsaltyȱthanȱtheȱopenȱocean).ȱInȱthisȱaspectȱ theyȱareȱsimilarȱtoȱmanyȱestuariesȱinȱareasȱsuchȱasȱTexasȱandȱpartsȱofȱtheȱMediterraneanȱSea.ȱ Forȱtheȱpurposesȱofȱtheseȱguidelines,ȱweȱwillȱconsiderȱthatȱtheȱheadȱofȱanȱestuaryȱisȱtheȱlandwardȱlimit,ȱ orȱwhereȱyouȱcanȱnoȱlongerȱseeȱanyȱtidalȱvariation,ȱandȱtheȱmouthȱisȱthatȱpartȱwhereȱeitherȱtheȱsalinityȱisȱ indistinguishableȱfromȱthatȱofȱtheȱsea,ȱorȱthereȱisȱsomeȱformȱofȱestuarineȱbarrierȱorȱsandbar.ȱ Weȱcanȱdevelopȱanȱunderstandingȱofȱtheȱprocessesȱthatȱaffectȱanȱestuaryȱbyȱlookingȱatȱtwoȱtypesȱofȱ classification.ȱWeȱcanȱexamineȱtheȱestuaryȱandȱassessȱitsȱsalinityȱregimeȱ(positiveȱorȱnegative).ȱWeȱcanȱ alsoȱclassifyȱitȱaccordingȱtoȱtheȱmajorȱforcesȱ(tides,ȱriverȱflows,ȱevaporationȱandȱwaves)ȱthatȱhaveȱshapedȱ it.ȱUnderstandingȱtheȱtypeȱofȱestuaryȱweȱareȱlookingȱatȱallowsȱusȱtoȱunderstandȱwhatȱhappensȱtoȱwaterȱ thatȱentersȱitȱandȱwhereȱtheȱdifferentȱhabitatsȱwillȱoccur.ȱ Po si ti ve and n eg ative es tu aries Theȱclassicalȱestuaryȱhasȱfreshwaterȱenteringȱtheȱheadȱofȱtheȱestuary,ȱwhichȱgraduallyȱbecomesȱsaltierȱ closerȱtoȱtheȱocean.ȱThisȱisȱconsideredȱtoȱbeȱaȱ‘positive’ȱestuary.ȱTheseȱestuariesȱrequireȱenoughȱ freshwaterȱenteringȱtoȱnotȱonlyȱdiluteȱtheȱseawater,ȱbutȱalsoȱtoȱalsoȱmakeȱupȱforȱtheȱeffectȱofȱevaporationȱ fromȱtheȱestuaryȱsurfaceȱitself.ȱPositiveȱestuariesȱareȱcommonȱinȱareasȱwhereȱthereȱisȱhighȱrainfallȱandȱ lowȱevaporation,ȱbutȱcanȱexistȱinȱlowȬrainfallȱareasȱwhereȱaȱsmallȱriverȱisȱfedȱfromȱanȱextremelyȱlargeȱ catchment.ȱInȱSouthȱAustraliaȱpositiveȱestuariesȱoccurȱinȱtheȱSouthȱEast,ȱonȱKangarooȱIsland,ȱandȱwouldȱ haveȱoccurredȱinȱtheȱpastȱinȱpartsȱofȱtheȱRiverȱMurrayȱestuary,ȱinȱLakesȱAlexandrinaȱandȱAlbertȱ(Geddesȱ andȱHall,ȱ1990).ȱ SouthȱAustralianȱestuariesȱareȱmoreȱcommonlyȱ‘negative’ȱestuaries,ȱwhereȱtheȱamountȱofȱfreshwaterȱ enteringȱtheȱestuaryȱasȱrainȱorȱrunoffȱisȱlessȱthanȱtheȱamountȱofȱfreshwaterȱlostȱasȱevaporationȱfromȱtheȱ estuaryȱsurface.ȱTheȱsalinityȱgradientȱinȱnegativeȱestuariesȱvariesȱfromȱhypersalineȱtoȱeusalineȱ(marineȱ salinity).ȱTheȱCoorongȱisȱaȱwellȬknownȱnegativeȱestuary.ȱ The OzEs tu aries class ifi cation Thereȱareȱseveralȱdifferentȱclassificationȱsystemsȱforȱestuaries.ȱMostȱseekȱtoȱclassifyȱtheȱestuaryȱonȱtheȱ basisȱofȱtidalȱinfluenceȱ(isȱthereȱaȱlargeȱorȱsmallȱexchangeȱofȱseawaterȱdaily?)ȱandȱrainfallȱ(isȱthereȱaȱlargeȱ riverȱorȱrainfallȱinputȱandȱwhatȱisȱtheȱevaporationȱlike?).ȱȱ GeoscienceȱAustraliaȱhasȱproducedȱaȱCDȱROMȱaboutȱAustralianȱestuaries,ȱdesignedȱforȱeducationalȱuse,ȱ thatȱincludesȱaȱclassificationȱsystemȱforȱestuariesȱ(commonlyȱcalledȱtheȱOzEstuariesȱclassification,ȱ developedȱbyȱHeapȱetȱal,ȱ2001).ȱTheȱsystemȱclassifiesȱestuariesȱintoȱthreeȱmajorȱtypes,ȱdependingȱonȱ whetherȱtheyȱareȱdominatedȱbyȱtheȱtidesȱ(mainlyȱfoundȱinȱnorthernȱAustraliaȱwhereȱtidesȱareȱlarge),ȱbyȱ riversȱ(theseȱareȱdeltaȱestuaries)ȱorȱbyȱwavesȱ(whereȱaȱsandyȱbarrierȱofȱsomeȱkindȱisȱformedȱbyȱwaves).ȱ Eachȱofȱtheseȱgroupsȱcanȱbeȱsubdivided,ȱprovidingȱsixȱsubclasses:ȱtideȱdominatedȱdeltas,ȱwaveȱ dominatedȱdeltas,ȱwaveȱdominatedȱestuaries,ȱstrandȱplains,ȱandȱtideȱdominatedȱestuariesȱandȱtidalȱflats.ȱȱ 5 ESTUARINE MONITORING GUIDANCE MANUAL Aȱquickȱsummaryȱofȱtheseȱclassesȱfollows.ȱIfȱyouȱwishȱtoȱunderstandȱtheȱclassificationȱmethod,ȱyouȱ shouldȱobtainȱaȱcopyȱofȱtheȱCDȱROMȱAustralianȱEstuariesȱandȱCoastalȱWaterwaysȱfromȱGeoscienceȱ Australia.ȱ Tideȱd om i nat edȱd e lta ȱ Deltasȱareȱriverȱdominated,ȱsoȱtheyȱcanȱhaveȱstrongȱfreshwaterȱinfluence.ȱThisȱmayȱbeȱonlyȱepisodic,ȱinȱ theȱcaseȱofȱSouthȱAustralianȱdeltas.ȱTheȱlargeȱquantityȱofȱsiltȱtheyȱcarryȱtoȱtheȱseaȱisȱdepositedȱaroundȱ theȱmouthȱofȱtheȱdelta,ȱcausingȱtheȱcharacteristicȱwedgeȬȱshapedȱdeltaȱtoȱformȱ(forȱexampleȱatȱtheȱLightȱ River,ȱinȱGulfȱStȱVincent).ȱTheȱdailyȱtidalȱinfluenceȱinȱaȱtideȬdominatedȱdeltaȱrapidlyȱmixesȱtheȱsaltyȱandȱ freshȱwater.ȱȱ W aveȱd om i na ted ȱd e lt a ȱ Whereȱwavesȱareȱstrongȱenoughȱtoȱdepositȱsedimentȱinȱtheȱmainȱbasinȱofȱoldȱestuaries,ȱwaveȱdominatedȱ deltasȱmayȱform.ȱThisȱtypeȱofȱestuaryȱmayȱbeȱseenȱinȱSouthȱAustraliaȱatȱtheȱOnkaparingaȱestuary.ȱTheȱ influenceȱofȱtheȱtideȱisȱnotȱlargeȱandȱsoȱtheseȱdeltasȱmayȱbecomeȱstratified,ȱwithȱaȱwedgeȱofȱsaltȱwaterȱ underlyingȱtheȱfresherȱsurfaceȱwater.ȱȱ W aveȱd om i na ted ȱe st u aryȱ Wavesȱareȱstrongȱmoversȱofȱcoarseȱsandyȱsediments,ȱandȱwhereȱwaveȱenergiesȱareȱstrongȱenoughȱtheyȱ canȱproduceȱaȱbarrierȱnearȱtheȱmouthȱofȱanȱestuary.ȱAȱwellȬknownȱSouthȱAustralianȱexampleȱisȱtheȱ MurrayȱMouthȱandȱCoorong.ȱTheȱsandyȱobstructionsȱtendȱtoȱattenuateȱtheȱtideȱinsideȱtheȱestuary,ȱ resultingȱinȱstratificationȱorȱpartialȱmixingȱofȱtheȱfreshȱandȱtidalȱwaterȱinȱtheȱlagoon–likeȱareaȱbehindȱtheȱ sandȱbarrier.ȱ St rand ȱp l a in ȱ Strandȱplainȱestuariesȱareȱtinyȱlagoonsȱwithȱminimalȱfreshwaterȱinfluence.ȱTheȱsandȱbarrierȱisȱkeptȱinȱ placeȱbyȱtheȱwavesȱandȱthereȱisȱsoȱsmallȱaȱfreshwaterȱflowȱthatȱtheȱsandȱmayȱcloseȱtheȱopeningȱ completely.ȱTheseȱestuariesȱmayȱonlyȱbeȱopenȱtoȱtheȱseaȱforȱpartȱofȱtheȱyear,ȱduringȱtheȱperiodȱofȱhighestȱ freshwaterȱflow.ȱTheȱsalinityȱgradientȱinȱtheseȱsmallȱestuariesȱisȱspreadȱoverȱtime,ȱasȱwellȱasȱspace,ȱasȱ theȱwaterȱbecomesȱmoreȱsalineȱoverȱsummer,ȱandȱlessȱsoȱoverȱwinter.ȱTheȱlackȱofȱwave,ȱtidalȱorȱriverȱ flowsȱinȱtheȱlagoonȱcanȱallowȱtheȱwaterȱtoȱdevelopȱsalinity,ȱtemperatureȱandȱdissolvedȱoxygenȱ stratifications.ȱ Tideȱd om i nat edȱ est uary ȱ Whereȱfreshwaterȱflowsȱareȱlow,ȱtideȱdominatedȱestuariesȱmayȱdevelop.ȱTheȱnorthernȱpartsȱofȱSpencerȱ GulfȱandȱGulfȱStȱVincentȱmayȱbeȱconsideredȱtoȱbeȱessentiallyȱestuariesȱthatȱdrainȱtheȱaridȱlandsȱtoȱtheȱ north.ȱTheȱtracksȱofȱoldȱriversȱ(paleorivers)ȱrunȱintoȱtheȱnorthernȱgulfs,ȱandȱshowȱonȱtheȱsurfaceȱasȱ chainsȱofȱsaltȱlakes.ȱFreshwaterȱdrainageȱintoȱtheȱgulfsȱalongȱtheseȱdepressionsȱisȱfairlyȱrare.ȱTideȱ dominatedȱestuariesȱhaveȱobviousȱtides,ȱwellȬmixedȱsalinitiesȱandȱextensiveȱmangroveȱsystems.ȱȱ T i d a lȱ f l a t sȱ SmallȱtidalȱflatȱestuariesȱareȱveryȱcommonȱinȱSouthȱAustralia,ȱparticularlyȱinȱtheȱtwoȱgulfsȱ(First,ȱSecondȱ andȱThirdȱCreeksȱatȱPortȱPirie,ȱtheȱPortȱRiver/BarkerȱInletȱestuary,ȱGawlerȱRiverȱandȱPortȱBroughtonȱ estuariesȱareȱaȱfew).ȱTheyȱareȱfoundȱinȱareasȱwithȱhighȱtidalȱinfluenceȱandȱtendȱtoȱdrainȱrightȱoutȱatȱlowȱ tide.ȱTheirȱsmallȱsizeȱandȱtheȱstrongȱtidalȱinfluenceȱtendȱtoȱresultȱinȱtheȱsalinityȱbeingȱwellȱmixed.ȱWithȱ theȱlowȱrainfallȱandȱaridȱconditions,ȱmanyȱofȱtheseȱestuariesȱareȱextremelyȱnegative,ȱwithȱsalinitiesȱinȱtheȱ landwardȱpartsȱofȱtheȱestuaryȱfrequentlyȱexceedingȱthatȱofȱseawater.ȱTheseȱsmallȱestuariesȱusuallyȱhaveȱ aȱriparianȱzoneȱofȱmangrovesȱbackedȱwithȱsaltmarsh.ȱȱ 6 ESTUARINE MONITORING GUIDANCE MANUAL Estuaries—values and impacts Estuarineȱareasȱareȱnurseriesȱforȱfishȱandȱlardersȱforȱmigratoryȱbirds.ȱTheȱvariedȱestuarineȱhabitatsȱformȱ naturalȱfiltersȱandȱbuffersȱbetweenȱtheȱlandȱandȱtheȱsea.ȱȱ Aȱtypicalȱpatternȱofȱhabitats,ȱidentifiedȱfirstȱbyȱChapmanȱ(1960),ȱisȱfoundȱinȱlowȱenergyȱcoastalȱareasȱinȱ Australia.ȱTheȱdiagramȱbelowȱ(Figureȱ1)ȱisȱaȱsimpleȱwayȱtoȱvisualiseȱtheȱhabitatsȱthatȱmayȱbeȱfoundȱinȱ theȱmarineȱandȱbrackishȱpartsȱofȱanȱestuary,ȱinȱwetȱorȱdryȱpartsȱofȱAustralia.ȱ ȱ Figure 1—Habitat zones along estuaries Whileȱeachȱhabitatȱmayȱappearȱtoȱbeȱquiteȱseparateȱfromȱtheȱothers,ȱtheyȱareȱactuallyȱmutuallyȱ dependant.ȱForȱexample,ȱseagrassesȱrequireȱclearȱwater,ȱandȱmangrovesȱandȱsaltmarshesȱactȱasȱfiltersȱtoȱ clarifyȱrunoffȱfromȱtheȱland.ȱAtȱtheȱsameȱtime,ȱmangrovesȱandȱsaltmarshesȱwillȱerodeȱwhereȱwaterȱ movementȱisȱhigh,ȱandȱseagrassȱbedsȱactȱasȱdampersȱtoȱreduceȱwaterȱmovementȱinȱtheȱnearȱshoreȱarea.ȱ 7 ESTUARINE MONITORING GUIDANCE MANUAL Thereforeȱitȱisȱimportantȱtoȱmanageȱestuarineȱhabitatsȱasȱaȱwhole,ȱratherȱthanȱconcentratingȱonȱaȱsingleȱ habitat.ȱ MajorȱpressureȱhasȱbeenȱappliedȱtoȱtheȱterrestrialȱestuarineȱhabitatsȱinȱtheȱmoreȱurbanȱpartsȱofȱSouthȱ Australia.ȱForȱexample,ȱinȱAdelaideȱtheȱsupratidalȱareasȱhaveȱbeenȱalmostȱentirelyȱdeveloped,ȱtheȱreedȱ swampsȱfromȱHindmarshȱtoȱPortȱAdelaideȱareȱnoȱlongerȱinȱevidence,ȱandȱmuchȱofȱtheȱupperȱsaltmarshȱ hasȱbeenȱconvertedȱtoȱindustrialȱlandȱinȱtheȱWingfieldȱdistrict.ȱEvenȱsomeȱofȱtheȱmangroveȱareasȱinȱtheȱ NorthȱArmȱareaȱhaveȱbeenȱimpoundedȱandȱdeveloped.ȱ Apartȱfromȱdevelopmentȱpressures,ȱestuarineȱhabitatsȱcomeȱunderȱpressureȱfromȱpollutingȱeffluentȱ outfalls,ȱsedimentationȱasȱaȱresultȱofȱoverȱclearingȱinȱupperȱcatchments,ȱtheȱoverfishingȱofȱfishȱandȱ invertebrateȱstocks,ȱandȱtheȱchangesȱtoȱhydrologyȱthatȱcomeȱwithȱsealingȱsurfaces,ȱdammingȱrivers,ȱ divertingȱflows,ȱandȱbuildingȱdikesȱandȱseawalls.ȱ ȱ ȱ ȱ 8 ESTUARINE MONITORING GUIDANCE MANUAL Working in the field—safety & environment Safetyȱisȱtheȱmostȱimportantȱconsiderationȱinȱconductingȱfieldwork.ȱMostȱsafetyȱissuesȱcanȱbeȱaddressedȱ withȱaȱmodicumȱofȱforethought.ȱGroupȱleadersȱneedȱtoȱvisitȱsitesȱbeforeȱtakingȱgroupsȱintoȱtheȱfield,ȱtoȱ assessȱanyȱpossibleȱrisks.ȱGroupȱleadersȱshouldȱdiscussȱtheȱarea,ȱtheȱactivitiesȱandȱanyȱsafetyȱ requirementsȱwithȱtheȱrestȱofȱtheȱgroupȱbeforeȱtakingȱthemȱoutȱtoȱtheȱsite.ȱSpecialȱtrainingȱ(suchȱasȱhowȱ toȱhandleȱbrokenȱglassȱorȱtreatȱaȱspillȱofȱreagents)ȱshouldȱbeȱrehearsedȱbeforeȱfieldȱactivitiesȱareȱ attempted.ȱȱ Theȱdetailsȱofȱwhatȱtrainingȱisȱrequiredȱwillȱdependȱonȱtheȱactivitiesȱbeingȱundertaken,ȱandȱtheȱskillȱandȱ ageȱofȱtheȱparticipants.ȱEachȱgroupȱleaderȱneedsȱtoȱassessȱtheirȱgroup’sȱparticularȱneeds.ȱSomeȱbasicȱ fieldworkȱsafetyȱandȱenvironmentalȱcareȱrulesȱareȱpresentedȱbelow.ȱTheyȱrevolveȱaroundȱtheseȱ principles:ȱ – ensuringȱyouȱdoȱnotȱfindȱyourselfȱinȱstrifeȱwithȱnoȱhelpȱavailableȱ – dressingȱappropriatelyȱ – beingȱawareȱofȱdangerousȱsituationsȱ – treatingȱyourselfȱandȱothersȱwithȱconsiderationȱ – leavingȱasȱlittleȱevidenceȱasȱpossibleȱbehindȱyou.ȱ E nsu re he lp is avai lable – workȱwithȱatȱleastȱoneȱotherȱpersonȱȱ – letȱsomebodyȱknowȱwhereȱyouȱareȱgoingȱandȱwhatȱtimeȱyouȱexpectȱtoȱreturnȱ – carryȱaȱmobileȱphone,ȱorȱradioȱ(ifȱoutȱofȱmobileȱreceptionȱzones)ȱȱ – carryȱaȱfirstȱaidȱkit.ȱȱ D r e s s a p p ro p ri a t e l y – wearȱappropriateȱclothingȱandȱfootwearȱtoȱsuitȱtheȱareaȱyouȱareȱmonitoring;ȱforȱexample,ȱyourȱ footwearȱmayȱneedȱtoȱwithstandȱimmersion,ȱhaveȱgoodȱgrip,ȱandȱprotectȱagainstȱsharpȱobjectsȱȱ – longȱsleeves,ȱsunscreenȱandȱaȱhatȱwillȱpreventȱsunburnȱ – insectȱrepellentȱmayȱbeȱnecessaryȱatȱcertainȱtimesȱofȱtheȱyearȱ – useȱglovesȱforȱhandlingȱlitter.ȱ Be aware of dangerous situ ations – checkȱtheȱtideȱtimetableȱbeforeȱgoingȱintoȱwetȱareas,ȱorȱyouȱmayȱbeȱtrappedȱbyȱtheȱtideȱ – bridgeȬbasedȱactivitiesȱmayȱbeȱdangerous—theȱwaterȱmayȱbeȱdeepȱandȱpassingȱtrafficȱmayȱalsoȱ beȱaȱhazard.ȱSafetyȱjacketsȱensureȱyouȱcanȱbeȱseenȱ – aȱseaworthyȱboatȱisȱoneȱwhereȱtheȱrunningȱgearȱisȱinȱgoodȱorder,ȱthereȱisȱappropriateȱsafetyȱ equipmentȱandȱthereȱareȱlifeȱjacketsȱforȱeachȱpassenger.ȱNeverȱleaveȱshoreȱwithȱanythingȱlessȱ – followȱboatingȱsafetyȱcodesȱ – mangalsȱandȱsaltmarshesȱhaveȱunevenȱterrain,ȱdenseȱgrowthȱandȱillȬdefinedȱwaterways.ȱAlwaysȱ useȱestablishedȱpathsȱwhereȱpossible.ȱDressȱbrightly,ȱsoȱthatȱyouȱcanȱbeȱfoundȱmoreȱeasilyȱifȱyouȱ fallȱorȱbecomeȱlost.ȱAvoidȱmuddyȱpatchesȱinȱmangalsȱandȱ‘rottenȱspots’ȱinȱsaltmarshes.ȱWearȱ tightȬfittingȱwetsuitȱbooteesȱratherȱthanȱwellingtonsȱinȱmuddyȱareas,ȱasȱyouȱareȱlessȱlikelyȱtoȱloseȱ them.ȱ T r e a t y o u rs e lf , a n d ot h e rs , w i th con s i d e ra t i o n – neverȱpushȱsomeoneȱwhoȱisȱtiredȱorȱcoldȱtoȱcontinueȱworkingȱ – carryȱwater,ȱandȱdrinkȱfrequently.ȱ 9 ESTUARINE MONITORING GUIDANCE MANUAL Leave as little eviden ce as possible behind you – transectȱandȱotherȱmarkersȱshouldȱonlyȱbeȱplacedȱwhereȱtheyȱcannotȱcauseȱdamageȱtoȱboatȱ propellers,ȱandȱshouldȱbeȱmadeȱofȱwood,ȱnotȱsteelȱȱ – removeȱanyȱrubbishȱthatȱyouȱhaveȱcreatedȱ – usedȱsampleȱreagentsȱshouldȱbeȱpouredȱintoȱaȱliddedȱbucketȱthatȱhasȱkittyȱlitterȱinȱit.ȱSealȱitȱandȱ takeȱitȱhomeȱwithȱyouȱtoȱputȱoutȱwithȱtheȱsolidȱwaste.ȱ 10 ESTUARINE MONITORING GUIDANCE MANUAL Working in the field—permits & politeness Thereȱareȱotherȱaspectsȱtoȱconsiderȱwhenȱworkingȱinȱtheȱfield.ȱYouȱmayȱneedȱaȱpermitȱtoȱconductȱ monitoringȱactivitiesȱinȱaȱnumberȱofȱsituations.ȱ NativeȱvegetationȱisȱprotectedȱoutsideȱtheȱimmediateȱAdelaideȱarea,ȱandȱonȱallȱCrownȱLands.ȱAnyȱ activitiesȱwhereȱlivingȱorȱdeadȱplantȱmatterȱisȱremovedȱ(forȱidentificationȱorȱforȱmeasurement)ȱmayȱ requireȱaȱpermitȱunderȱtheȱNativeȱVegetationȱActȱ1991,ȱwhichȱisȱadministeredȱbyȱtheȱNationalȱParksȱandȱ WildlifeȱServiceȱ(NPWS).ȱAdditionally;ȱthisȱauthorityȱisȱresponsibleȱforȱadministeringȱallȱconservationȱ reservesȱinȱtheȱstate,ȱmostȱofȱwhichȱhaveȱrestrictionsȱonȱtheȱtypesȱofȱactivitiesȱthatȱmayȱtakeȱplaceȱwithinȱ them.ȱ AdviceȱonȱwhetherȱaȱpermitȱisȱrequiredȱforȱaȱspecificȱactivityȱunderȱtheȱNativeȱVegetationȱAct,ȱorȱtheȱ NationalȱParksȱandȱWildlifeȱActȱ1973,ȱmayȱbeȱobtainedȱfromȱtheȱNPWSȱonȱ(08)ȱ8204ȱ8776.ȱȱ MangroveȱplantsȱareȱadditionallyȱprotectedȱunderȱtheȱFisheriesȱActȱ1982.ȱThisȱActȱalsoȱprovidesȱ protectionȱforȱmanyȱmarineȱandȱestuarineȱareasȱsuchȱasȱAquaticȱReserves.ȱIntertidalȱareasȱthatȱareȱpartȱ ofȱAquaticȱReservesȱmayȱhaveȱotherȱrestrictionsȱonȱtakingȱinvertebratesȱorȱotherȱanimals.ȱTheȱactivitiesȱ thatȱcanȱbeȱconductedȱinȱtheseȱareasȱareȱdefinedȱinȱtheȱFisheriesȱActȱRegulations,ȱandȱonȱboardsȱerectedȱ atȱboatȱrampsȱinȱaquaticȱreserves.ȱAdviceȱonȱpermitsȱthatȱmayȱbeȱrequiredȱunderȱtheȱFisheriesȱActȱmayȱ beȱobtainedȱfromȱPrimaryȱIndustriesȱandȱResourcesȱSAȱ(PIRSA)ȱonȱ(08)ȱ8226ȱ0222.ȱ Groundwaterȱpiezometersȱlessȱthanȱ2.5ȱmetresȱdeepȱdoȱnotȱrequireȱaȱpermitȱunderȱtheȱWaterȱResourcesȱ Actȱ1997;ȱhowever,ȱdeeperȱmonitoringȱboresȱdo.ȱShallowȱpiezometersȱputȱinȱplaceȱforȱmonitoringȱ groundwaterȱchangesȱinȱsaltmarshesȱareȱquiteȱlikelyȱtoȱbeȱlocatedȱonȱCrownȱland,ȱsoȱalthoughȱnoȱpermitȱ mayȱbeȱrequiredȱunderȱtheȱWaterȱResourcesȱAct,ȱpermissionȱmayȱstillȱbeȱrequiredȱfromȱtheȱNPWS.ȱ Politenessȱisȱaȱbasicȱrequirementȱforȱestablishingȱaȱmonitoringȱprogramȱthatȱisȱwellȱsupportedȱbyȱtheȱ community.ȱManyȱpeopleȱuseȱestuaries.ȱSomeȱpeopleȱdependȱonȱlocalȱestuariesȱtoȱmakeȱaȱliving,ȱ (adjacentȱlandowners,ȱcommercialȱfishermen)ȱandȱotherȱpeopleȱenjoyȱrecreationalȱactivitiesȱinȱestuaries.ȱ Theȱfollowingȱadviceȱwillȱensureȱthatȱgroupsȱconductingȱmonitoringȱdoȱnotȱcauseȱoffenceȱtoȱotherȱ estuaryȱusers.ȱ – alwaysȱobtainȱpermissionȱfromȱlandownersȱbeforeȱenteringȱtheirȱlandȱ – leaveȱgatesȱexactlyȱtheȱwayȱyouȱfoundȱthemȱ – doȱnotȱlitterȱtheȱmonitoringȱsiteȱ – beȱawareȱthatȱuntendedȱmarkersȱareȱjustȱanotherȱformȱofȱlitter—removeȱtemporaryȱmarkersȱasȱ soonȱasȱtheȱactivityȱisȱfinished.ȱPermanentȱmarkersȱforȱinterȬannualȱcomparisonȱstudiesȱshouldȱ beȱwellȱmaintained,ȱmarkedȱwithȱaȱcontactȱlabel,ȱandȱplacedȱsoȱtheyȱareȱnotȱhazardousȱtoȱotherȱ usersȱ – manyȱareasȱaroundȱestuariesȱareȱownedȱbyȱtheȱCrown,ȱbutȱmanagedȱbyȱdifferentȱauthoritiesȱ (localȱgovernment,ȱCoastȱProtectionȱBoard,ȱNWPSȱorȱPIRSA).ȱToȱsetȱupȱaȱmonitoringȱprogramȱonȱ theseȱareas,ȱdiscussȱwhatȱyouȱwouldȱlikeȱtoȱdoȱwithȱtheȱmanagingȱauthorityȱ – beȱawareȱthatȱtheȱnaturalȱvariationȱthatȱoccursȱinȱmanyȱhabitatsȱfromȱoneȱyearȱtoȱanotherȱcanȱbeȱ large,ȱandȱitȱmayȱtakeȱseveralȱyearsȱofȱmonitoringȱbeforeȱitȱisȱpossibleȱtoȱidentifyȱwhetherȱaȱ particularȱeventȱhasȱaȱnaturalȱorȱmanȬinducedȱcause.ȱYouȱmayȱwishȱtoȱseekȱexpertȱadviceȱwhenȱ interpretingȱyourȱresultsȱ – ifȱprivateȱbodiesȱsuchȱasȱindustriesȱorȱresearchersȱprovideȱyouȱwithȱinformationȱtoȱhelpȱyouȱ understandȱyourȱestuary,ȱneverȱprovideȱthatȱinformationȱtoȱothersȱwithoutȱaskingȱpermissionȱ fromȱtheȱpeopleȱwhoȱgaveȱitȱtoȱyou.ȱ 11 ESTUARINE MONITORING GUIDANCE MANUAL Designing your monitoring program Theȱrangeȱofȱactivitiesȱprovidedȱbyȱtheȱframeworkȱisȱlargeȱsoȱasȱtoȱallowȱasȱmanyȱcommunityȱgroupsȱasȱ possibleȱtoȱbecomeȱinvolvedȱinȱmonitoringȱtheirȱestuarineȱareas.ȱNotȱallȱtheȱactivitiesȱwillȱbeȱsuitableȱforȱ yourȱgroup,ȱorȱforȱtheȱhabitatsȱthatȱoccurȱinȱyourȱspecificȱestuary.ȱȱ Beforeȱdoingȱanything,ȱitȱisȱimportantȱtoȱdevelopȱaȱmonitoringȱprogramȱthatȱyourȱgroupȱwillȱbeȱableȱtoȱ undertake.ȱItȱneedsȱtoȱcontainȱsufficientȱactivitiesȱtoȱprovideȱtheȱgroupȱwithȱanswersȱtoȱtheirȱquestionsȱ aboutȱtheirȱestuaryȱwhileȱmaintainingȱinterest,ȱbutȱalsoȱmustȱtakeȱupȱonlyȱtheȱtimeȱthatȱyouȱhaveȱ available.ȱTheȱdecisionsȱyouȱwillȱneedȱtoȱmakeȱinclude:ȱȱ – decidingȱwhatȱyouȱwishȱtoȱknowȱaboutȱyourȱestuaryȱ – choosingȱwhichȱactivitiesȱareȱsuitableȱforȱyourȱgroupȱandȱyourȱestuaryȱ – decidingȱhowȱmuchȱtimeȱyouȱhaveȱavailableȱtoȱmonitorȱyourȱestuaryȱ – decidingȱonȱhowȱfrequentlyȱyouȱwillȱmonitorȱeachȱparameterȱyouȱhaveȱchosenȱ(monthly?ȱ quarterly?ȱannually?)ȱȱ – determiningȱinȱwhatȱorderȱtoȱundertakeȱtheȱactivitiesȱyouȱhaveȱchosenȱ – understandingȱwhatȱlevelȱofȱdataȱqualityȱyouȱwishȱtoȱstriveȱfor.ȱ Choosing activities Inȱgeneral,ȱitȱisȱadvisableȱtoȱselectȱactivitiesȱfromȱeachȱofȱtheȱgroupsȱprovidedȱinȱtheȱmonitoringȱ framework.ȱTheȱcoreȱactivitiesȱfromȱtheȱsectionsȱSamplingȱsiteȱobservationsȱandȱUnderstandingȱestuarineȱ processesȱareȱtheȱbasicȱactivitiesȱthatȱallȱmonitoringȱprogramsȱcouldȱideallyȱbeȱbuiltȱon.ȱThen,ȱifȱwaterȱ qualityȱisȱanȱinterest,ȱconsiderȱtheȱcoreȱactivitiesȱfromȱtheȱChemicalȱassessmentsȱsectionȱofȱtheȱframework.ȱ Atȱthisȱpoint,ȱconsiderȱwhetherȱyourȱgroupȱwouldȱlikeȱtoȱunderstandȱestuarineȱprocessesȱandȱwaterȱ qualityȱinȱmoreȱdetail.ȱIfȱso,ȱthereȱareȱextensionȱactivitiesȱavailableȱinȱthoseȱsectionsȱthatȱwillȱdeepenȱ yourȱunderstandingȱofȱtheseȱaspectsȱofȱyourȱestuary.ȱ Theȱbiologicalȱsectionsȱofȱtheȱframeworkȱhaveȱfewerȱcoreȱactivities,ȱandȱmoreȱextensionȱactivities.ȱThisȱisȱ becauseȱmanyȱofȱtheseȱactivitiesȱreflectȱhabitatsȱthatȱmay,ȱorȱmayȱnot,ȱbeȱpresentȱinȱyourȱestuary.ȱTheȱ activitiesȱyourȱgroupȱchoosesȱshouldȱbeȱbasedȱonȱtheȱhabitatsȱinȱyourȱestuary,ȱorȱinȱtheȱpartȱofȱyourȱ estuaryȱthatȱyouȱareȱsampling.ȱManyȱofȱtheseȱactivitiesȱrequireȱmoreȱtimeȱtoȱconduct,ȱmoreȱcomplexȱ equipment,ȱorȱaȱhigherȱskillȱlevel.ȱȱ TheȱcoreȱactivitiesȱdescribedȱinȱLookingȱatȱvegetationȱandȱhabitatȱandȱinȱObservingȱanimalȱlifeȱmayȱbeȱ conductedȱbyȱpeopleȱfromȱaȱwideȱrangeȱofȱageȱandȱskillȱlevels.ȱTheseȱcoreȱactivitiesȱareȱalsoȱsuitableȱforȱ mostȱestuaries.ȱȱ W h i ch a c t i v i ty f i rs t ? Whenȱconductingȱfieldwork,ȱtimingȱandȱtheȱorderȱofȱactivitiesȱmatter.ȱTryȱtoȱmonitorȱatȱtheȱsameȱtimeȱofȱ day,ȱandȱalsoȱattemptȱtoȱmonitorȱatȱtheȱsameȱpartȱofȱtheȱtidalȱcycle.ȱTheȱlatterȱisȱnotȱalwaysȱpossible,ȱbutȱ atȱleastȱkeepȱaȱrecordȱofȱtheȱstateȱofȱtheȱtide.ȱ Toȱobserveȱsomeȱaspectsȱofȱwaterȱquality,ȱsuchȱasȱdissolvedȱoxygen,ȱwhichȱchangeȱoverȱtheȱperiodȱofȱaȱ day,ȱyouȱmayȱconsiderȱaȱoneȬoffȱsamplingȱeventȱthatȱmonitorsȱthatȱparameterȱonȱanȱhourlyȱ(orȱevenȱ moreȱfrequent)ȱbasis,ȱsoȱthatȱyouȱmayȱseeȱtheȱdiurnalȱvariationȱthatȱoccursȱinȱyourȱestuary.ȱ Whenȱmonitoring,ȱitȱisȱimportantȱthatȱtheȱresultȱofȱundertakingȱoneȱactivityȱdoesȱnotȱinfluenceȱtheȱnextȱ activity.ȱForȱexample,ȱwaterȱdepthȱtestingȱusingȱaȱSecchiȱdiskȱorȱmeasuringȱstaffȱstirsȱupȱsediment,ȱ makingȱtheȱlocationȱunsuitableȱforȱtestingȱwaterȱclarityȱ(Secchiȱdepth)ȱorȱforȱcollectingȱsamplesȱforȱ measuringȱsuspendedȱsolids.ȱAsȱaȱgeneralȱrule,ȱonȱarrivingȱatȱaȱsite,ȱundertakeȱtheȱobservationalȱ activitiesȱfirstȱ(weather,ȱsurfaceȱwaterȱconditions,ȱetc).ȱThenȱcollectȱanyȱclearȱwaterȱsamplesȱthatȱyouȱwillȱ needȱforȱmeasuringȱwaterȱchemistry.ȱFinally,ȱundertakeȱthoseȱactivitiesȱthatȱmayȱdisturbȱtheȱbenthicȱ sediments.ȱȱ 12 ESTUARINE MONITORING GUIDANCE MANUAL D a ta q u a li t y Credibilityȱdoesnȇtȱmeanȱhavingȱtheȱmostȱexactingȱtechniques.ȱItȱmeansȱdeliveringȱonȱyourȱ promises,ȱnoȱmatterȱhowȱsmallȱorȱlargeȱtheyȱare.ȱ ȱMegȱKerr,ȱRIȱRiverȱRescueȱ Goodȱresultsȱrequireȱcleanlyȱcollectedȱsamples,ȱwellȬmaintainedȱequipmentȱandȱcarefulȱmethods.ȱ ComparativeȱstudiesȱofȱWaterwatchȱcommunityȬcollectedȱdataȱandȱprofessionallyȬcollectedȱdataȱinȱ Victoriaȱ(Nicholsonȱetȱal.,ȱ2002)ȱandȱTasmaniaȱ(Otley,ȱ2000)ȱhaveȱshownȱthatȱdespiteȱdifferencesȱinȱtheȱ levelȱofȱscientificȱtraining,ȱdataȱsetsȱcollectedȱbyȱtheȱtwoȱgroupsȱwereȱcomparableȱforȱtheȱparametersȱofȱ electricalȱconductivityȱ(salinity)ȱandȱpH.ȱTheȱTasmanianȱstudyȱfoundȱthatȱtheȱtwoȱgroupsȱcouldȱproduceȱ similarȱresultsȱinȱaȱwholeȬofȬcatchmentȱsurveyȱforȱaȱwideȱrangeȱofȱparameters.ȱInȱVictoria,ȱturbidityȱandȱ phosphorusȱdataȱcollectedȱbyȱcommunityȱgroupsȱwasȱlessȱconsistentȱandȱhadȱaȱcoarserȱresolutionȱthanȱ dataȱcollectedȱbyȱprofessionalȱgroups.ȱ Groupsȱcollectȱdataȱforȱdifferentȱreasons.ȱSomeȱgroupsȱmayȱbeȱmonitoringȱanȱareaȱtoȱdetectȱlongȬtermȱ trends,ȱothersȱmayȱbeȱtryingȱtoȱtrackȱdownȱaȱpointȬsourceȱpollutant,ȱandȱstillȱothersȱmayȱbeȱusingȱtheirȱ monitoringȱasȱpartȱofȱanȱoverallȱeducationȱprogram.ȱTheȱlevelȱofȱconfidenceȱthatȱeachȱgroupȱwillȱrequireȱ inȱanyȱsingleȱmeasurementȱwillȱthereforeȱbeȱdifferent.ȱItȱisȱimportantȱtoȱdecideȱwhatȱlevelȱofȱdataȱ confidenceȱyourȱgroupȱrequiresȱandȱisȱcapableȱofȱproducing,ȱandȱthenȱdesignȱtheȱqualityȱplanȱsectionȱofȱ yourȱgroup’sȱmonitoringȱprogramȱtoȱmeetȱthatȱlevel.ȱ WaterwatchȱSAȱhasȱdevelopedȱaȱrangeȱofȱdataȱqualityȱcategoriesȱforȱcommunityȱwaterȱmonitoringȱthatȱ groupsȱmayȱchooseȱtoȱaimȱfor.ȱTheseȱlevelsȱare:ȱ 1 AdvancedȱKnownȱQualityȱData—whereȱtestȱmethodsȱareȱchosenȱthatȱareȱcomparableȱinȱ resolutionȱtoȱmethodsȱusedȱbyȱotherȱwaterȱdataȱusers,ȱwhereȱsamplingȱfrequencyȱisȱatȱleastȱ sixȱtimesȱannually,ȱwhereȱequipmentȱisȱcalibratedȱforȱeachȱevent,ȱresultsȱareȱreportedȱdirectlyȱ fromȱtheȱequipmentȱandȱreportingȱunitsȱareȱrecorded,ȱdataȱonȱsamplingȱconditionsȱareȱ reported,ȱduplicatesȱareȱtested,ȱgroupȱtrainingȱoccursȱannuallyȱandȱ‘mysteryȱsolutions’ȱareȱ attemptedȱtwiceȱannually.ȱTheseȱdataȱmayȱbeȱmadeȱavailableȱtoȱexternalȱusers,ȱwithȱtheȱ provisionȱofȱtheȱtoleranceȱlimitsȱ(theȱmethodȱresolution)ȱforȱeachȱparameter.ȱ 2ȱȱȱStandardȱKnownȱQualityȱData—whereȱtestȱmethodsȱareȱchosenȱthatȱareȱcomparableȱinȱȱȱȱȱȱȱȱȱȱȱȱȱȱȱȱ resolutionȱtoȱmethodsȱusedȱbyȱotherȱwaterȱdataȱusers,ȱwhereȱsamplingȱfrequencyȱisȱatȱleastȱ fourȱtimesȱannually,ȱwhereȱequipmentȱisȱcalibratedȱforȱeachȱevent,ȱresultsȱareȱreportedȱ directlyȱfromȱtheȱequipmentȱandȱreportingȱunitsȱareȱrecorded,ȱdataȱonȱsamplingȱconditionsȱ areȱreported,ȱandȱgroupȱtrainingȱandȱ‘mysteryȱsolutions’ȱareȱattemptedȱonceȱannually.ȱTheȱ dataȱmayȱbeȱmadeȱavailableȱtoȱexternalȱusersȱforȱtrendȱmonitoringȱpurposes,ȱwithȱtheȱ provisionȱofȱwiderȱtoleranceȱlimitsȱ(rȱtwiceȱtheȱmethodȱresolution)ȱforȱeachȱparameter.ȱ 3 UnknownȱQualityȱData—whereȱtestȱmethodsȱareȱchosenȱthatȱareȱofȱcoarserȱresolutionȱthanȱ otherȱwaterȱusersȱmayȱrequire,ȱinitialȱgroupȱtrainingȱhasȱbeenȱattended,ȱdataȱsheetsȱareȱfilledȱ inȱtoȱtheȱbestȱofȱtheȱgroup’sȱabilityȱbutȱmayȱnotȱbeȱcomplete,ȱandȱwhereȱsamplingȱfrequencyȱ isȱatȱleastȱonceȱannually.ȱTheseȱdataȱareȱofȱexcellentȱvalueȱinȱtheȱclassroomȱsituationȱwhereȱ theȱmeasurementsȱmayȱbeȱincorporatedȱintoȱpartsȱofȱtheȱmathematics,ȱscience,ȱandȱsocietyȱ andȱenvironmentȱcurricula.ȱ S am p l eȱc o l le ct ing ȱ Howȱsamplesȱareȱcollectedȱandȱhandledȱmayȱinfluenceȱyourȱresults.ȱTheȱfollowingȱpointsȱwillȱhelpȱyouȱ toȱcollectȱsamplesȱthatȱareȱrepresentativeȱofȱtheȱwaterȱbodyȱyouȱareȱexamining:ȱ – sampleȱbeforeȱundertakingȱactivitiesȱthatȱwillȱdisturbȱanyȱsedimentȱinȱtheȱwaterȱbody.ȱ – toȱcollectȱsamplesȱforȱimmediateȱtestingȱuseȱcleanȱpolyethyleneȱorȱPETȱbottles.ȱEnsureȱthereȱareȱ noȱdetergentȱresiduesȱifȱusingȱcleanedȱbottles.ȱForȱsamplesȱtoȱbeȱanalysedȱbyȱaȱlaboratory,ȱ discussȱwhatȱbottles,ȱcleaningȱprotocolsȱandȱsamplingȱmethodsȱareȱrequiredȱwithȱtheȱlaboratory.ȱ Labelȱanyȱbottlesȱyouȱareȱsendingȱtoȱaȱlaboratoryȱpriorȱtoȱsamplingȱwithȱaȱsampleȱnumber,ȱtheȱ location,ȱdateȱandȱanalysesȱrequired.ȱ 13 ESTUARINE MONITORING GUIDANCE MANUAL – sampleȱdirectlyȱintoȱtheȱsamplingȱbottleȱorȱjarȱunlessȱaȱmixedȱsampleȱisȱnecessary,ȱinȱwhichȱcaseȱ ensureȱthatȱtheȱlargerȱmixingȱcontainerȱhasȱbeenȱpreparedȱinȱtheȱsameȱmannerȱasȱtheȱsamplingȱ container.ȱȱ – youȱshouldȱrinseȱtheȱsamplingȱcontainerȱinȱtheȱsampleȱstreamȱseveralȱtimes,ȱunlessȱtheȱbottleȱhasȱ beenȱpreparedȱforȱsendingȱtoȱaȱlaboratory.ȱInȱwhichȱcase,ȱtheȱbottleȱmayȱcontainȱpreservativeȱthatȱ mustȱbeȱretained,ȱsoȱshouldȱnotȱbeȱrinsedȱinȱtheȱsampleȱstream.ȱ – whenȱsampling,ȱsubmergeȱtheȱbottleȱcarefully.ȱTheȱmouthȱofȱtheȱbottleȱshouldȱbeȱheldȱbelowȱtheȱ bodyȱofȱtheȱbottleȱandȱplungedȱintoȱtheȱwaterȱbeforeȱbeingȱtippedȱupȱtoȱallowȱwaterȱtoȱenter.ȱTheȱ mouthȱshouldȱremainȱatȱleastȱ200ȱmmȱbelowȱtheȱsurface.ȱAȱsamplingȱdipperȱsimplifiesȱobtainingȱ aȱcleanȱsubȬsurfaceȱsample.ȱ – inȱflowingȱwater,ȱalwaysȱensureȱthatȱyourȱhandȱisȱdownstreamȱofȱtheȱcollectingȱbottleȇsȱmouthȱ andȱthatȱtheȱmouthȱofȱtheȱbottleȱisȱfacingȱintoȱtheȱcurrent.ȱ – itȱisȱnecessaryȱthatȱtheȱpersonȱtakingȱtheȱsamplesȱuseȱtheirȱdiscretionȱwhileȱcollecting,ȱsoȱasȱnotȱtoȱ takeȱaȱnonȬrepresentativeȱsample.ȱWhenȱsampling,ȱavoidȱdislodgingȱbenthicȱalgalȱmats,ȱscrapingȱ againstȱtheȱestuaryȱfloorȱorȱjettyȱorȱbridgeȱpylons,ȱorȱotherwiseȱdisturbingȱtheȱsediment.ȱCareȱ shouldȱbeȱtakenȱsoȱthatȱnoȱlargerȱanimalsȱorȱalgaeȱmatsȱareȱincludedȱinȱtheȱsample,ȱunlessȱthatȱisȱ whatȱyouȱwishȱtoȱsample.ȱ – whenȱsamplingȱwaters,ȱflowingȱorȱwellȬmixedȱwaterȱisȱpreferable,ȱbutȱthisȱmayȱnotȱalwaysȱbeȱ available,ȱparticularlyȱwhenȱveryȱstillȱconditionsȱcauseȱstratificationȱinȱestuaries.ȱInȱthisȱcase,ȱ attemptȱtoȱcollectȱthreeȱequallyȱsizedȱsamples,ȱfromȱtheȱsurface,ȱmidzoneȱandȱdeeperȱwaters,ȱmixȱ themȱtogetherȱandȱsubȬsampleȱtheȱmixture,ȱorȱcollectȱseveralȱseparateȱsamplesȱifȱyouȱwishȱtoȱ determineȱaȱprofile.ȱȱ – whereȱpossible,ȱsampleȱinȱtheȱmiddleȱofȱtheȱestuary,ȱorȱatȱleastȱinȱdeeperȱwater.ȱȱ – onceȱcollected,ȱmeasureȱtheȱparametersȱyouȱareȱinterestedȱinȱimmediately.ȱLeavingȱtheȱwaterȱ standingȱinȱaȱsmallȱcontainerȱwillȱallowȱsomeȱparametersȱtoȱchangeȱ(temperatureȱ&ȱdissolvedȱ oxygen,ȱforȱexample).ȱ – ifȱcollectingȱfromȱaȱboat,ȱshutȱoffȱtheȱmotorȱbeforeȱarrivingȱatȱtheȱsiteȱandȱdriftȱtheȱlastȱfewȱmetresȱ toȱreduceȱdisturbanceȱatȱtheȱsampleȱsite.ȱAlwaysȱsampleȱupstreamȱfromȱtheȱmotor,ȱinȱcaseȱanyȱ hydrocarbonsȱhaveȱescapedȱ(thisȱisȱparticularlyȱtheȱcaseȱwithȱolderȱwaterȬcooledȱtwoȬstrokeȱ engines).ȱ Equipmentȱcalibration ȱ Theȱoldȱsayingȱthatȱaȱworkmanȱisȱonlyȱasȱgoodȱasȱhisȱtoolsȱappliesȱequallyȱtoȱmeasuringȱwaterȱqualityȱasȱ itȱdoesȱtoȱanyȱotherȱactivity.ȱToȱensureȱtheȱinformationȱyouȱcollectȱisȱasȱaccurateȱasȱpossible,ȱlookȱafterȱ yourȱequipmentȱandȱcalibrateȱitȱregularly.ȱAdviceȱonȱdifferentȱtypesȱofȱequipmentȱisȱgivenȱbelow:ȱ – calibrateȱandȱmaintainȱyourȱequipmentȱbeforeȱgoingȱoutȱtoȱaȱsamplingȱsiteȱandȱuseȱanȱequipmentȱ checklist.ȱTheȱmiddleȱofȱtheȱestuaryȱisȱnoȱplaceȱtoȱdiscoverȱsomethingȱwon’tȱworkȱproperlyȱorȱ thatȱyouȱhaveȱleftȱsomethingȱatȱhome.ȱȱ – ifȱyouȱareȱusingȱaȱmeter,ȱreferȱtoȱtheȱinstructionȱmanualȱthatȱcameȱwithȱtheȱmeterȱtoȱdiscoverȱhowȱ toȱcalibrateȱit.ȱYouȱwillȱmostȱlikelyȱneedȱtoȱuseȱbufferȱsolutionsȱofȱknownȱconductivityȱandȱpH.ȱȱ – checkȱwhatȱtypeȱofȱbatteryȱtheȱmeterȱuses,ȱandȱcarryȱaȱspareȱoneȱinȱtheȱmeter’sȱcarryȱcase.ȱȱ – coil,ȱdon’tȱkink,ȱmeterȱcables.ȱ – treatȱyourȱmeterȱwithȱrespect.ȱItȱisȱaȱdelicateȱinstrument.ȱAlwaysȱcarryȱaȱmeterȱinȱaȱcarryȱcaseȱorȱ box.ȱDoȱnotȱletȱitȱrattleȱaroundȱlooseȱinȱtheȱbottomȱofȱyourȱsamplingȱtubȱorȱboat.ȱ – rinseȱtheȱprobesȱofȱyourȱmeterȱinȱfreshȱwaterȱafterȱsamplingȱinȱtheȱestuary,ȱandȱstoreȱthemȱasȱ recommendedȱinȱtheȱmeter’sȱmanual.ȱ – checkȱtheȱexpiryȱdatesȱonȱallȱtestȱkitȱreagentsȱbeforeȱtakingȱthemȱoutȱinȱtheȱfield.ȱOldȱreagentsȱ mayȱgiveȱinaccurateȱresults.ȱ – ifȱyouȱareȱusingȱpHȱpapers,ȱbeȱawareȱthatȱtheyȱmayȱbecomeȱinaccurateȱifȱtheyȱareȱold,ȱorȱmayȱ takeȱlongerȱtoȱreact.ȱBeforeȱgoingȱoutȱtoȱtheȱsamplingȱsite,ȱtryȱaȱfewȱpapersȱinȱpHȱbufferȱsolutionsȱ 14 ESTUARINE MONITORING GUIDANCE MANUAL ofȱ4ȱorȱ10,ȱtoȱcheckȱtheirȱaccuracyȱandȱhowȱlongȱtheyȱtakeȱtoȱreact.ȱIfȱtheyȱareȱunacceptable,ȱthrowȱ theȱpackȱawayȱandȱopenȱaȱnewȱpack.ȱ – thermometersȱandȱhydrometersȱrequireȱcheckingȱonceȱaȱyearȱagainstȱaȱcertifiedȱinstrumentȱ (returnȱthemȱtoȱyourȱWaterwatchȱcoordinator).ȱBeingȱglass,ȱtheseȱinstrumentsȱusuallyȱbreakȱveryȱ obviously,ȱbutȱoccasionallyȱoneȱmayȱdevelopȱaȱvisuallyȱundetectableȱcrackȱthatȱinterferesȱwithȱitsȱ accuracy.ȱ – treatȱyourȱglassȱitemsȱofȱequipmentȱwithȱrespect.ȱCarryȱthemȱinȱaȱpaddedȱboxȱandȱhandleȱthemȱ gently.ȱBrokenȱinstrumentsȱshouldȱbeȱplacedȱinȱaȱsharpsȱcontainerȱforȱdisposal.ȱ – refractometersȱmayȱbeȱcalibratedȱusingȱdistilledȱwaterȱandȱaȱstandardȱsaltȱsolution.ȱ – refractometersȱhaveȱaȱglassȱprismȱandȱtheseȱmayȱbeȱdamagedȱeasily.ȱTheȱprismȱmayȱbreak,ȱorȱ mayȱbeȱjoltedȱoutȱofȱalignment.ȱShouldȱthisȱhappen,ȱtheȱinstrumentȱmayȱneedȱreplacing,ȱorȱ repairingȱatȱanȱopticalȱspecialist.ȱ – theȱdistance–markedȱcordsȱonȱSecchiȱdisksȱmayȱstretchȱorȱshrinkȱwithȱuseȱandȱrepeatedȱwettingȱ andȱdrying.ȱBeforeȱgoingȱoutȱintoȱtheȱfield,ȱcheckȱtheȱaccuracyȱofȱtheȱmarkingsȱonȱtheȱcordȱusingȱ aȱmetalȱtape.ȱ Method s, ȱ r ec o rd sȱ and ȱ qu a lityȱp lan sȱ Rawȱdataȱmayȱbeȱveryȱmisleading.ȱToȱuseȱinformationȱcorrectly,ȱyouȱneedȱtoȱknowȱhowȱitȱwasȱcollected,ȱ whatȱexactlyȱwasȱbeingȱmeasuredȱandȱtheȱprecisionȱwithȱwhichȱitȱwasȱmeasured,ȱamongstȱotherȱthings.ȱ Aȱdataȱqualityȱplanȱisȱtheȱdocumentȱthatȱprovidesȱendȱusersȱofȱyourȱdataȱwithȱinformationȱonȱthoseȱ items.ȱ Theȱusualȱdataȱqualityȱplanȱcontainsȱaȱmapȱshowingȱyourȱsamplingȱsite,ȱaȱsamplingȱtimetable,ȱaȱlistȱofȱ equipmentȱandȱtestȱmethodsȱthatȱyouȱuse,ȱdetailsȱofȱtheȱmethodȱrangesȱandȱresolutions,ȱdetailsȱofȱtheȱ reportingȱunitsȱforȱeachȱmethod,ȱdetailsȱonȱhowȱyouȱcheckȱforȱdataȱqualityȱ(calibrationȱofȱequipment,ȱ duplicateȱsamples,ȱetc)ȱandȱdetailsȱonȱtheȱtrainingȱyourȱgroupȱhasȱreceived.ȱTheȱdocumentȱdoesȱnotȱ needȱtoȱbeȱlong,ȱandȱyourȱWaterwatchȱcoordinatorȱcanȱassistȱyouȱinȱdevelopingȱit.ȱ Theȱotherȱrecordsȱyouȱneedȱtoȱmaintainȱareȱtheȱsamplingȱrecords.ȱAlwaysȱattemptȱtoȱfillȱinȱtheȱsiteȱ recordsȱwithȱasȱmuchȱdetailȱasȱyouȱcanȱatȱtheȱtimeȱofȱsampling.ȱKeepȱthemȱinȱaȱfileȱorȱfolder,ȱsoȱthatȱyouȱ doȱnotȱmislayȱthemȱasȱyouȱbuildȱupȱaȱcollectionȱofȱsheets.ȱRememberȱtoȱsendȱinȱcopiesȱofȱyourȱsiteȱ recordsȱtoȱyourȱWaterwatchȱcoordinatorȱifȱyouȱwishȱtheȱdataȱtoȱbeȱavailableȱforȱothersȱtoȱuse.ȱȱ Finally,ȱconsiderȱwhatȱyouȱareȱtryingȱtoȱdetermine,ȱandȱuseȱmethodsȱthatȱareȱappropriate.ȱItȱisȱnotȱ usuallyȱnecessaryȱtoȱmeasureȱlocationsȱonȱaȱ50–mȱtransectȱtoȱtheȱmillimetre.ȱItȱisȱpointlessȱtryingȱtoȱ measureȱnutrientsȱthatȱoccurȱinȱtheȱrangeȱofȱ0–0.5ȱmg/Lȱusingȱtestsȱwithȱmeasuringȱstepsȱofȱ1ȱmg/L.ȱ ȱ ȱ ȱ 15 ESTUARINE MONITORING GUIDANCE MANUAL Selecting your monitoring sites Theȱsitesȱthatȱyouȱsampleȱwillȱdependȱonȱwhetherȱyouȱareȱattemptingȱtoȱmonitorȱanȱentireȱestuaryȱ(aȱ hugeȱprojectȱinȱaȱlargeȱestuary,ȱbutȱfeasibleȱforȱaȱsingleȱgroupȱthatȱisȱmonitoringȱaȱsmallȱestuarineȱcreek,ȱ orȱwhenȱdoingȱspecificȱactivitiesȱsuchȱasȱmappingȱtheȱsalinityȱgradient),ȱorȱwhetherȱyourȱgroupȱisȱ responsibleȱforȱmonitoringȱjustȱoneȱsiteȱasȱpartȱofȱmanyȱgroupsȱmonitoringȱanȱestuary.ȱȱ Bearingȱinȱmindȱthatȱwaterȱsamplesȱtakenȱatȱtheȱshallowȱedgesȱofȱanȱestuaryȱareȱlikelyȱtoȱbeȱ unrepresentative,ȱselectȱsitesȱwhereȱthereȱisȱsafeȱaccessȱtoȱdeeperȱwater.ȱInȱlongȱestuariesȱinȱurbanȱareas,ȱ aȱseriesȱofȱbridgesȱmayȱprovideȱusefulȱplatforms,ȱandȱallowȱyouȱtoȱgainȱsamplesȱfromȱbothȱsidesȱandȱtheȱ middleȱofȱeachȱspan.ȱJettiesȱandȱwharfsȱareȱotherȱoptionsȱthatȱshouldȱbeȱconsidered.ȱȱ Shouldȱyouȱbeȱmonitoringȱanȱentireȱestuaryȱ(suchȱasȱsmallȱestuarineȱtidalȱcreeks),ȱattemptȱtoȱhaveȱ samplingȱsitesȱthatȱcoverȱtheȱspectrumȱofȱsalinitiesȱfoundȱinȱtheȱestuary.ȱ Ifȱyourȱgroupȱhasȱaȱspecificȱinterest,ȱyourȱsamplingȱlocationsȱmayȱreflectȱthat.ȱForȱexample,ȱsomeȱgroupsȱ mayȱbeȱinterestedȱinȱremediationȱofȱtidalȱwetlands,ȱandȱtheseȱgroupsȱmayȱsampleȱtheȱwetlandȱtheyȱareȱ workingȱon.ȱOtherȱgroupsȱmayȱwishȱtoȱmonitorȱtheȱeffectsȱofȱstormwaterȱorȱeffluentȱoutfallsȱ(byȱ mappingȱturbidityȱplumes,ȱforȱexample).ȱ ȱ ȱ ȱ 16 Eȱ S T U A R I N E MONITORING GUIDANCE MANUAL Sampling site observations ȱ ȱ ȱ ȱ ȱ ȱ ȱ 17 ESTUARINE MONITORING GUIDANCE MANUAL Recording weather details Weatherȱaffectsȱtheȱestuarineȱenvironmentȱitselfȱandȱyourȱabilityȱtoȱ recordȱaccurateȱinformationȱaboutȱtheȱestuary.ȱȱ Hotȱweatherȱmayȱreduceȱtheȱabilityȱofȱestuarineȱwatersȱtoȱholdȱ dissolvedȱoxygen,ȱwindyȱconditionsȱmayȱstirȱupȱsediment,ȱandȱ heavyȱrainȱmayȱintroduceȱplumesȱofȱturbidȱrunoff.ȱAdditionally,ȱitȱ mayȱbeȱdifficultȱtoȱobtainȱaccurateȱSecchiȱdiskȱmeasurementsȱinȱ strongȱglareȱorȱwhenȱtheȱsurfaceȱofȱtheȱwaterȱisȱchoppy.ȱ Inȱgeneral,ȱcalmȱconditionsȱareȱbestȱforȱmonitoring.ȱAvoidȱ monitoringȱinȱstrongȱstorms,ȱasȱthereȱareȱmonitoringȱ representativenessȱasȱwellȱasȱsafetyȱissuesȱtoȱconsider.ȱ Activity: 3 Core Age: All Time: 1 Why? The weather affects many other parameters being measured in a monitoring program, so should be included to aid interpretation of the collected data. What you may need: – siteȱrecordȱformȱ – Beaufortȱscaleȱ – thermometerȱ – weatherȱrecordsȱforȱpreviousȱfewȱdaysȱfromȱtheȱBureauȱofȱMeteorologyȱ(BOM)ȱwebsiteȱ <www.bom.gov.au>.ȱ What to do: Onȱyourȱsiteȱrecordȱform,ȱrecordȱtheȱweatherȱatȱtheȱtimeȱyouȱareȱvisitingȱyourȱsamplingȱsite.ȱ Youȱmayȱrecordȱwhetherȱitȱisȱsunnyȱorȱcloudy,ȱandȱestimateȱhowȱmuchȱofȱtheȱskyȱisȱcoveredȱbyȱcloud.ȱIfȱ itȱisȱraining,ȱrecordȱthis,ȱandȱalsoȱrecordȱwhetherȱitȱhasȱrainedȱearlierȱthatȱday.ȱ Youȱmayȱuseȱtheȱsameȱthermometerȱthatȱyouȱuseȱforȱmeasuringȱwaterȱtemperatureȱtoȱmeasureȱairȱ temperature.ȱPlaceȱtheȱthermometerȱinȱaȱshadedȱdryȱplaceȱandȱallowȱitȱtoȱequilibrateȱforȱfiveȱminutes,ȱ thenȱreadȱtheȱtemperature.ȱWetȱthermometersȱorȱthoseȱinȱtheȱsunȱwillȱgiveȱerroneousȱreadings.ȱȱ AȱversionȱofȱAdmiralȱBeaufort’sȱwindȱspeedȱscaleȱhasȱbeenȱprovidedȱinȱAppendixȱ2,ȱtoȱallowȱyouȱtoȱ estimateȱwindȱspeed.ȱTheȱversionȱusedȱinȱthisȱmanualȱisȱtheȱoneȱthatȱincludesȱindicatorsȱthatȱoneȱmayȱ seeȱonȱland,ȱandȱisȱmoreȱsuitableȱforȱuseȱinȱestuariesȱthanȱtheȱversionsȱthatȱareȱusedȱonȱtheȱopenȱsea.ȱ IfȱyouȱcanȱobtainȱtheȱweatherȱrecordsȱforȱtheȱpreviousȱfewȱdaysȱfromȱtheȱBOMȱwebsite,ȱattachȱtheseȱ behindȱyourȱsiteȱrecordȱsheet.ȱTheȱweatherȱleadingȱupȱtoȱyourȱsamplingȱeventȱmayȱhaveȱaȱbearingȱonȱ whatȱyouȱdiscoverȱonȱsite.ȱForȱexample,ȱaȱseriesȱofȱhot,ȱhumidȱnightsȱcanȱresultȱinȱveryȱlowȱoxygenȱ levelsȱjustȱbeforeȱsunriseȱandȱcanȱsometimesȱcauseȱfishȱdeaths.ȱ ȱ ȱ ȱ 18 ESTUARINE MONITORING GUIDANCE MANUAL Recording time and tide height What you may need: – siteȱrecordȱformȱ Activity: – timepieceȱ Age: All – tideȱtimetable.ȱ Time: 1 What to do: Onȱyourȱsiteȱrecordȱform,ȱrecordȱtheȱtimeȱyouȱvisitȱyourȱsamplingȱ site.ȱLookȱupȱtheȱtideȱtimetableȱforȱtheȱnearestȱtideȱstationȱtoȱyourȱ monitoringȱsiteȱandȱdecideȱwhatȱstageȱtheȱtideȱisȱat.ȱIsȱitȱhighȱtide,ȱ lowȱtide,ȱebbȱ(falling)ȱtide,ȱorȱfloodȱ(rising)ȱtide?ȱ Alsoȱrecordȱtheȱtimeȱandȱheightȱofȱtheȱtideȱthatȱoccurredȱ immediatelyȱbeforeȱyourȱvisit,ȱandȱtheȱtimeȱandȱheightȱofȱtheȱnextȱ tideȱthatȱwillȱoccur.ȱ 19 3 Core Why? The state of the tide affects many other parameters being measured in a monitoring program, so should be included to aid interpretation of the collected data. ESTUARINE MONITORING GUIDANCE MANUAL Recording odours and odd things What you may need: – Activity: siteȱrecordȱform.ȱ 3 Core Age: All What to do: Ifȱyourȱsiteȱsmells,ȱrecordȱthisȱonȱyourȱsiteȱrecord,ȱalongȱwithȱaȱ descriptionȱofȱtheȱsmell.ȱDoesȱitȱsmellȱlikeȱfish,ȱsewage,ȱnewȬcutȱ grass,ȱrottenȱeggs,ȱoverȬripeȱfruitȱorȱfuel?ȱ Asȱwellȱasȱsmell,ȱtakeȱaȱnoteȱofȱanythingȱelseȱunusual—areȱnewȱ pipesȱdischargingȱnearȱtheȱsite?ȱIsȱthereȱanȱoilȱslickȱonȱtheȱwater?ȱOilȱ slicksȱfromȱpollutionȱareȱusuallyȱbrightlyȱcoloured,ȱbutȱsometimesȱinȱ summer,ȱtheȱfaintestȱslickȱofȱrainbowȱcoloursȱonȱtheȱwaterȱsurfaceȱmayȱȱ beȱfromȱtheȱoilȱinȱmangroveȱleaves.ȱ Time: 1 Why? Algal blooms, some effluents and anoxic conditions all produce various odours. Theȱpresenceȱofȱdeadȱfishȱandȱcrabsȱisȱanȱindicatorȱthatȱshouldȱbeȱrecorded,ȱasȱwellȱasȱtheȱpresenceȱofȱ scumȱorȱfoamȱonȱtheȱwater,ȱrustȬcolouredȱoozeȱfromȱtheȱsoilȱnextȱtoȱtheȱwater,ȱtheȱpresenceȱofȱlotsȱofȱ floatingȱrubbish,ȱorȱunusuallyȱturbidȱ(muddy)ȱwater.ȱ ȱ 20 ESTUARINE MONITORING GUIDANCE MANUAL Recording water depth What you may need: – Secchiȱdiskȱorȱdepthȱstaffȱ(directionsȱforȱmakingȱaȱSecchiȱdiskȱ areȱincludedȱinȱAppendixȱ6)ȱ Activity: – siteȱrecordȱform.ȱ Age: All 3 Core To ensure good results: Time: 1 Asȱmeasuringȱtheȱwaterȱdepthȱmayȱdisturbȱtheȱsediment,ȱthisȱ measurementȱshouldȱbeȱmadeȱafterȱallȱotherȱmeasurementsȱhaveȱ beenȱtaken.ȱ Why? The depth of water may affect other parameters being measured, such as temperature and dissolved oxygen. TheȱmarkedȱcordȱonȱaȱSecchiȱdiskȱmayȱstretchȱorȱshrinkȱoverȱtime,ȱsoȱ itȱshouldȱbeȱcheckedȱagainstȱaȱmetalȱruleȱbeforeȱusingȱitȱinȱtheȱfield.ȱ What to do: GentlyȱlowerȱtheȱSecchiȱdiskȱintoȱtheȱwaterȱuntilȱitȱjustȱtouchesȱtheȱ bottomȱofȱtheȱwaterway.ȱReadȱtheȱwaterȱdepthȱfromȱtheȱcord.ȱ Inȱshallowȱwater,ȱaȱmeasuringȱstaffȱmayȱbeȱusedȱinsteadȱofȱaȱSecchiȱdisk.ȱȱ Toȱpreventȱtheȱstaffȱdiggingȱintoȱanyȱsoftȱsediment,ȱtheȱstaffȱȱ shouldȱhaveȱaȱfootplateȱfitted.ȱ 21 Safety: Deep water may be dangerous. ESTUARINE MONITORING GUIDANCE MANUAL Recording surface water conditions Windsȱacrossȱtheȱsurfaceȱofȱtheȱestuaryȱhelpsȱmixȱtheȱfresherȱsurfaceȱ waterȱwithȱtheȱsaltierȱwaterȱbelow.ȱStratificationȱ(layering)ȱofȱ salinity,ȱtemperatureȱandȱdissolvedȱoxygenȱmayȱoccurȱinȱveryȱcalmȱ conditions.ȱLargeȱwindȬdrivenȱwavesȱindicateȱthatȱmuchȱmixingȱwillȱ beȱoccurring,ȱalongȱwithȱadditionalȱoxygenȱincorporation.ȱ 3 Core Age: All Time: 1 Why? The water surface conditions indicate how much mixing is occurring in the estuary. What you may need: – Activity: siteȱrecordȱform.ȱ What to do: Safety: Deep water may be dangerous. Avoid monitoring in rough conditions. Observeȱtheȱwaterȱsurface.ȱDoesȱitȱlook:ȱ – calmȱlikeȱaȱmirror?ȱ – ruffledȱorȱcoveredȱwithȱsmallȱscaleȬlikeȱripples?ȱ – coveredȱwithȱshortȱwaveletsȱthatȱdoȱnotȱhaveȱcrests?ȱ – moderatelyȱroughȱwithȱlongerȱwavesȱwithȱoccasionalȱwhiteȱ glassyȱfoamȱcrests?ȱ – quiteȱroughȱwithȱwavesȱwithȱ‘whiteȱhorses’ȱofȱfoamȱwhereȱ theirȱcrestsȱareȱbreaking?ȱ Recordȱtheȱwaterȱsurfaceȱconditionȱonȱtheȱsiteȱrecordȱform.ȱ ȱ ȱ 22 ESTUARINE MONITORING GUIDANCE MANUAL Recording water colour Waterȱoftenȱhasȱaȱslightȱdegreeȱofȱcolour,ȱandȱthisȱcanȱindicateȱtheȱ chemicalsȱdissolvedȱinȱit,ȱorganicȱmaterialsȱsuchȱasȱpeat,ȱbloomsȱofȱ phytoplankton,ȱandȱsoilȱerosion.ȱFieldȱmeasurementsȱofȱcolourȱ tendȱtoȱbeȱsubjective,ȱandȱaȱwideȱrangeȱofȱcolourȱscalesȱhaveȱbeenȱ developedȱforȱmeasuringȱcolourȱinȱwater,ȱrangingȱfromȱtheȱ platinumȱscale,ȱtoȱtheȱForel–UleȱscaleȱandȱtheȱBorgerȱandȱMunsellȱ colourȱsystems.ȱ Laboratoriesȱuseȱspectrophotometersȱtoȱdetermineȱtheȱhue,ȱ saturationȱandȱluminanceȱofȱcolouredȱwater,ȱtherebyȱobtainingȱaȱ quantitativeȱresult.ȱInȱtheȱfield,ȱoneȱobservesȱtheȱapparentȱcolourȱ ofȱwaterȱreflectedȱfromȱtheȱwhiteȱquadrantsȱofȱaȱSecchiȱdisk,ȱandȱ comparesȱtheȱcolourȱtoȱanȱarbitraryȱcolourȱchart,ȱsetȱofȱcolouredȱ glassȱstandards,ȱorȱsetȱofȱcolouredȱwaterȱvials.ȱ Activity: 3 Core Age: All Time: 1 Why? Water colour can indicate dissolved matter, the colour of the substrate, the presence of muddy sediments, floating oils or the presence of an algal bloom. Safety: Deep water can be dangerous. Aȱusefulȱsetȱofȱcolourȱdefinitionsȱhasȱrecentlyȱevolvedȱwithȱtheȱ adventȱofȱtheȱinternet.ȱToȱdefineȱcoloursȱusedȱonȱHTML–codedȱ webȱpagesȱthatȱwillȱbeȱdisplayedȱonȱTVȱandȱPCȱmonitors,ȱanȱRGBȱ (red,ȱgreen,ȱblue)ȱcolourȱcodeȱisȱused.ȱThisȱallowsȱpeopleȱworkingȱ allȱoverȱtheȱworldȱtoȱdefineȱcoloursȱtheȱsameȱway.ȱȱ TheȱcolourȱchartȱprovidedȱinȱAppendixȱ3ȱusesȱtheȱHTMLȬRGBȱ codes,ȱandȱhasȱaȱsimilarȱrangeȱofȱcoloursȱtoȱtheȱForel–Uleȱcolourȱ scaleȱthatȱisȱcommonlyȱusedȱinȱestuarineȱstudies.ȱ What you may need: – Secchiȱdiskȱ(directionsȱforȱmakingȱaȱSecchiȱdiskȱareȱincludedȱinȱAppendixȱ6)ȱ – siteȱrecordȱformȱ – waterȱcolourȱchart.ȱ To ensure good results: TheȱmarkedȱcordȱonȱaȱSecchiȱdiskȱmayȱstretchȱorȱshrinkȱoverȱtime,ȱsoȱtheȱmarkingsȱonȱtheȱcordȱshouldȱ beȱcheckedȱagainstȱaȱmetalȱruleȱbeforeȱusingȱtheȱSecchiȱdiskȱinȱtheȱfield.ȱ Ifȱyouȱreprintȱtheȱcolourȱchart,ȱcheckȱtheȱcolourȱvaluesȱagainstȱtheȱprintedȱchartȱinȱAppendixȱ3ȱofȱthisȱ manualȱtoȱensureȱtheyȱhaveȱreproducedȱcorrectly.ȱ SubmergeȱtheȱSecchiȱdiskȱinȱaȱshadyȱareaȱ(shadyȱsideȱofȱboat,ȱjettyȱorȱbridge)ȱifȱpossible,ȱtoȱreduceȱtheȱ effectȱofȱglare.ȱ What to do: GentlyȱlowerȱtheȱSecchiȱdiskȱintoȱtheȱwaterȱuntilȱtheȱdiskȱisȱoneȱmetreȱbelowȱtheȱsurface.ȱLookȱatȱtheȱ colourȱreflectingȱbackȱfromȱtheȱwhiteȱquadrantȱofȱtheȱdisk.ȱȱ Compareȱthisȱcolourȱwithȱyourȱcolourȱchartȱandȱrecordȱtheȱcolourȱcodeȱthatȱisȱclosestȱtoȱtheȱwaterȱcolourȱ reflectingȱbackȱfromȱtheȱSecchiȱdisk.ȱ 23 ESTUARINE MONITORING GUIDANCE MANUAL Measuring flow rate Flowsȱinȱestuariesȱareȱveryȱcomplex.ȱRecordingȱflowȱrateȱcanȱhelpȱ youȱdetermineȱwhetherȱerosionȱorȱaccretionȱofȱtheȱshorelineȱisȱ likelyȱtoȱoccur,ȱwhetherȱanȱareaȱisȱlikelyȱtoȱhostȱcertainȱtypesȱofȱ aquaticȱvegetation,ȱandȱ(ifȱtheȱflowȱrateȱisȱtakenȱinȱaȱstormwaterȱ drain,ȱeffluentȱoutfallȱorȱcreekȱthatȱisȱjoiningȱtheȱestuary)ȱtheȱloadȱ ofȱpollutantsȱthatȱisȱenteringȱtheȱestuary.ȱ Flowȱrateȱmayȱaffectȱturbidityȱorȱwaterȱclarity,ȱdissolvedȱoxygenȱ concentrationȱandȱstratification.ȱWhenȱexaminingȱfringingȱ wetlandsȱitȱisȱimportantȱtoȱrecordȱflowȱrateȱtoȱhelpȱdetermineȱ whetherȱtidalȱrestrictionsȱareȱoccurringȱinȱtheseȱvulnerableȱareas.ȱ Theȱrateȱofȱflowȱmayȱbeȱmultipliedȱbyȱtheȱcross–sectionalȱareaȱofȱ theȱwaterwayȱbeingȱmeasuredȱtoȱdetermineȱtheȱvolumeȱofȱwaterȱ passingȱaȱgivenȱpointȱoverȱaȱperiodȱofȱtime.ȱDetailsȱforȱcalculatingȱ thisȱareȱcontainedȱinȱtheȱbookȱWaterwatchȱAustraliaȱNationalȱ TechnicalȱManual,ȱVolumeȱ4–PhysicalȱandȱChemicalȱParametersȱ (WaterwatchȱAustraliaȱSteeringȱCommittee,ȱ2002).ȱ Activity: 3 Extension Age: All Time: 2 Why? The speed that water moves provides an indicator of the volume of stormwater, riverine or tidal flows in an estuary. Different habitats occur in areas of different flow rates. Safety: Fast flowing, or deep, water may be dangerous. What you may need: – activityȱrecordȱformȱ – severalȱorangesȱ – aȱnetȱonȱaȱpoleȱ – aȱ50Ȭmȱmeasuringȱtapeȱ – aȱstopwatch.ȱ To ensure good results: Theȱlongerȱtheȱdistanceȱyouȱtimeȱyourȱorangeȱover,ȱtheȱmoreȱlikelyȱyouȱareȱtoȱobtainȱanȱaccurateȱresult,ȱ soȱuseȱaȱminimumȱdistanceȱofȱ10ȱmetres,ȱbutȱpreferablyȱuseȱaȱ50Ȭmȱdistance.ȱChooseȱanȱareaȱwhereȱthereȱ areȱnoȱstructuresȱorȱrockyȱoutcropsȱjuttingȱintoȱtheȱflow,ȱasȱtheseȱwillȱmodifyȱtheȱflowȱaroundȱthem.ȱ What to do: Useȱyourȱtapeȱtoȱmarkȱtheȱdistanceȱyouȱwillȱmeasureȱtheȱflowȱrateȱover.ȱPlaceȱaȱpersonȱatȱtheȱbeginningȱ andȱendȱofȱtheȱdistance.ȱTheȱpersonȱatȱtheȱstartȱofȱtheȱdistanceȱshouldȱhaveȱaȱstopwatch.ȱTheȱpersonȱatȱ theȱendȱofȱtheȱdistanceȱshouldȱhaveȱaȱnetȱonȱaȱpoleȱforȱcatchingȱtheȱoranges.ȱȱ Placeȱanȱorangeȱinȱtheȱwaterȱupstreamȱofȱtheȱfirstȱperson.ȱAsȱtheȱorangeȱpassesȱtheȱfirstȱperson,ȱtheyȱ shouldȱstartȱtiming.ȱAsȱtheȱorangeȱpassesȱtheȱsecondȱpersonȱtheyȱshouldȱcallȱoutȱloudly,ȱthenȱattemptȱtoȱ netȱtheȱorange.ȱTheȱfirstȱpersonȱshouldȱstopȱtheȱwatchȱasȱsoonȱasȱtheyȱhearȱtheȱsecondȱpersonȱcallȱout.ȱ Repeatȱthisȱatȱleastȱthreeȱtimes.ȱTryȱtoȱuseȱtheȱsameȱorange,ȱbutȱasȱtheȱorangeȱisȱbiodegradable,ȱlosingȱ theȱoddȱoneȱ(ifȱtheȱflowȱisȱfast,ȱforȱexample)ȱisȱnotȱsignificant.ȱ Takeȱanȱaverageȱofȱtheȱreadings,ȱandȱuseȱthisȱasȱtheȱtimeȱinȱtheȱfollowingȱcalculation:ȱ Flow rate (m/sec) = distance travelled time taken u 0.9 where 0.9 is the correction factor because velocity varies between the middle and edge of a channel 24 Eȱ S T U A R I N E MONITORING GUIDANCE MANUAL Understanding estuarine processes ȱ 25 ESTUARINE MONITORING GUIDANCE MANUAL Measuring the water temperature Waterȱtemperatureȱisȱimportantȱforȱtheȱfunctioningȱofȱestuariesȱandȱ theȱecosystemsȱwithinȱthem.ȱItȱalsoȱinfluencesȱaȱnumberȱofȱchemicalȱ andȱphysicalȱparameters,ȱsoȱshouldȱbeȱmeasuredȱwheneverȱaȱwaterȱ qualityȱprogramȱisȱrunȱinȱanȱestuarineȱenvironment.ȱȱ Higherȱwaterȱtemperaturesȱdecreaseȱtheȱabilityȱofȱwaterȱtoȱholdȱ oxygen,ȱreduceȱtheȱdensityȱofȱtheȱwaterȱ(howȱheavyȱitȱis)ȱandȱmayȱ alsoȱincreaseȱtheȱchanceȱofȱanȱalgalȱbloom.ȱRapidȱtemperatureȱshiftsȱ affectȱtheȱhealthȱofȱmanyȱspeciesȱofȱaquaticȱanimals,ȱandȱmayȱstressȱ themȱconsiderably.ȱȱ Long–termȱtemperatureȱchangesȱmayȱhaveȱaȱconsiderableȱimpactȱ onȱestuarineȱecosystems.ȱWarmerȱwaterȱtemperaturesȱencourageȱ rapidȱgrowthȱofȱheatȬtolerantȱorganisms,ȱwhichȱoftenȱincludeȱ introducedȱspeciesȱbroughtȱinȱbyȱshipsȱfromȱtropicalȱports.ȱAsȱanȱ example,ȱPacificȱOysters,ȱintroducedȱforȱaquaculture,ȱwillȱoftenȱ reproduceȱinȱtheȱwarmȱshelteredȱwatersȱofȱestuaries,ȱwhereȱtheyȱ settleȱonȱrockyȱshoresȱandȱreduceȱtheȱspaceȱavailableȱforȱnativeȱ species.ȱ Activity: 3 Core Age: All Time: 1 Why? Temperature plays an important role in determining the amount of oxygen that can remain in the water, as well as affecting the reproductive status of many organisms. Warmer or cooler water than usual for an area may allow invasive species to gain a foothold. Lowȱtemperaturesȱslowȱdownȱgrowthȱandȱdecreaseȱfoodȱ availabilityȱtoȱfishȱandȱotherȱgrazingȱanimals.ȱȱ Temperature also controls the rate of many of the chemical processes that occur in an estuary. Factorsȱthatȱinfluenceȱwaterȱtemperatureȱincludeȱtimeȱofȱday,ȱ season,ȱdepthȱofȱwater,ȱflowȱrate,ȱtidalȱinfluence,ȱnearbyȱcoolingȱ waterȱoutfallsȱandȱtheȱlocationȱandȱdepthȱofȱtheȱsamplingȱpoint.ȱ Recordingȱtheseȱotherȱfactorsȱwhenȱtakingȱyourȱtemperatureȱ readingȱwillȱhelpȱensureȱthatȱyourȱdataȱisȱmoreȱusefulȱforȱtheȱ managementȱofȱyourȱestuary.ȱ Safety: Use alcohol thermometers, not mercury ones. Glass thermometers may break. Handle gently. Broken pieces should be placed in a sharps container. Thereȱareȱseveralȱmethodsȱusedȱtoȱmeasureȱwaterȱtemperature.ȱTheȱ mostȱcommonȱmethodsȱuseȱaȱglassȱorȱelectronicȱthermometer.ȱAȱ glassȱthermometerȱisȱtheȱcheaper.ȱAnȱaccurateȱelectronicȱ thermometerȱisȱquiteȱexpensiveȱinȱcomparison.ȱAccurateȱelectronicȱ temperatureȱsensorsȱareȱcommonlyȱfoundȱinȱmultiȬsensorȱCTPȱ meters,ȱwhereȱconductivity,ȱtemperatureȱandȱpHȱareȱmeasuredȱ together.ȱȱ Temperatureȱreadingsȱareȱusedȱtoȱinterpretȱotherȱparameters.ȱHigherȱwaterȱtemperatureȱinfluencesȱtheȱ abilityȱofȱwaterȱtoȱholdȱoxygen,ȱandȱmayȱinfluenceȱsalinityȱreadings.ȱReferȱtoȱtheȱactivitiesȱMeasuringȱ salinityȱandȱMeasuringȱdissolvedȱoxygenȱforȱmoreȱdetails.ȱ What you may need: – siteȱrecordȱformȱ – redȱspiritȱthermometerȱ – collectionȱbottleȱattachedȱtoȱaȱpoleȱforȱsampling.ȱ To ensure good results: Theȱthermometerȱshouldȱbeȱcalibratedȱyearlyȱagainstȱaȱcertifiedȱthermometer.ȱȱ Thermometersȱshouldȱbeȱcheckedȱpriorȱtoȱeveryȱuseȱforȱbubblesȱinȱtheȱredȱspiritȱorȱcracksȱinȱtheȱglass.ȱIfȱ bubblesȱorȱgapsȱareȱfoundȱinȱtheȱredȱspirit,ȱtheȱthermometerȱshouldȱbeȱ‘shakenȱdown’ȱuntilȱtheȱbubblesȱ orȱgapsȱdisappear.ȱIfȱcracksȱareȱfoundȱtheȱthermometerȱshouldȱbeȱreplaced.ȱ 26 ESTUARINE MONITORING GUIDANCE MANUAL What to do: Collectȱaȱwaterȱsampleȱfromȱatȱleastȱaȱmetreȱoutȱfromȱtheȱshoreȱ(preferablyȱfurtherȱout)ȱandȱfromȱ approximatelyȱ200–mmȱbelowȱtheȱsurface.ȱȱ Placeȱtheȱthermometerȱintoȱtheȱwaterȱsampleȱasȱsoonȱasȱitȱisȱcollectedȱandȱleaveȱtheȱthermometerȱinȱtheȱ waterȱuntilȱtheȱreadingȱstabilisesȱ(thisȱmayȱtakeȱupȱtoȱtwoȱminutes).ȱȱ RecordȱtheȱreadingȱonȱtheȱsiteȱrecordȱformȱasȱdegreesȱCelsiusȱ(qC)ȱȱ ȱ ȱ ȱ 27 ESTUARINE MONITORING GUIDANCE MANUAL Measuring pH pHȱisȱtechnicallyȱtheȱhydrogenȱpotentialȱofȱaȱliquid.ȱInȱcommonȱ languageȱitȱisȱaȱmeasureȱofȱtheȱacidityȱorȱalkalinity.ȱ pHȱrangesȱfromȱ1ȱtoȱ14.ȱWhenȱaȱliquidȱhasȱaȱpHȱofȱ7ȱitȱisȱconsideredȱ neutral.ȱAȱpHȱofȱlessȱthanȱ7ȱisȱacidicȱandȱmoreȱthanȱ7ȱisȱalkalineȱorȱ basic.ȱȱ TheȱpHȱvalueȱofȱwaterȱisȱcriticalȱtoȱtheȱsurvivalȱofȱmanyȱaquaticȱ plantsȱandȱanimals,ȱthusȱmonitoringȱofȱpHȱisȱanȱimportantȱpartȱofȱ allȱwaterȱqualityȱmonitoringȱprograms.ȱpHȱvaluesȱofȱwaterȱinȱtheȱ environmentȱmayȱvaryȱconsiderably.ȱPureȱwaterȱisȱneutral.ȱ FreshwaterȱstreamsȱoftenȱhaveȱaȱpHȱofȱbetweenȱ6.5ȱandȱ8.0.ȱ EstuarineȱwatersȱusuallyȱhaveȱaȱpHȱofȱbetweenȱ6.5ȱandȱ9.2ȱ(EPA,ȱ 2003).ȱ pHȱreadingsȱmayȱriseȱwithȱrisingȱsalinity,ȱasȱmanyȱofȱtheȱsaltsȱ foundȱinȱAustralianȱstreamsȱareȱalkaline.ȱHighȱpHȱreadingsȱmayȱ alsoȱindicateȱtheȱpresenceȱofȱanȱalgalȱbloom.ȱȱ Areasȱwithȱlargeȱamountsȱofȱpeatȱorȱorganicȱlitterȱinȱtheȱwaterȱcanȱ haveȱveryȱlowȱ(<ȱ5)ȱpHȱreadingsȱasȱaȱresultȱofȱhumicȱacidsȱreleasedȱ fromȱtheȱrottingȱorganicȱmatter.ȱȱ PollutionȱmayȱraiseȱorȱlowerȱpHȱvaluesȱoutsideȱtheirȱnormalȱlimits.ȱ Adelaideȱhasȱlargeȱareasȱofȱpotential,ȱandȱactual,ȱacidȱsulphateȱsoilsȱ thatȱareȱtheȱresultȱofȱdikingȱareasȱofȱsaltmarshȱawayȱfromȱtheȱsea.ȱ Theseȱareasȱareȱleachingȱandȱgeneratingȱveryȱacidȱgroundwaterȱ (oftenȱlessȱthanȱ4),ȱwhichȱthenȱenterȱourȱestuaries.ȱSomeȱindustrialȱ wastes,ȱparticularlyȱfromȱcausticȱplants,ȱcanȱbeȱhighlyȱalkaline,ȱandȱ theseȱareȱregularlyȱdischargedȱintoȱestuarineȱenvironments.ȱȱ Activity: 3 Core Age: All Time: 2 Why? The pH of water controls the solubility of many metals such as iron and copper. Low pH may indicate acid rain events or acid soil drainage. Values outside the range 5-10 indicate industrial pollution or cataclysmic events. Estuaries are normally between 6.5 and 8.5 (lower in their freshwater end, higher at the marine end). Safety: Dispose of all reagent/sample mixes appropriately. If water is suspected to be strongly acidic or alkaline avoid skin contact when handling samples. pHȱmayȱbeȱmeasuredȱusingȱpHȱstripsȱ(withȱaȱresolutionȱofȱ0.5ȱpHȱunits),ȱpHȱliquidȱtestȱkitsȱ(withȱaȱ resolutionȱofȱ0.2ȱunits)ȱorȱwithȱaȱprobeȱandȱmultiȬpurposeȱmeterȱ(withȱaȱresolutionȱofȱlessȱthanȱ0.1ȱpHȱ units).ȱTheȱpHȱstripsȱareȱtheȱcheapestȱandȱmostȱruggedȱoptionȱbutȱareȱlessȱpreciseȱthanȱtheȱotherȱtwoȱ methods.ȱSomeȱtypesȱofȱstripȱareȱaffectedȱbyȱsalinity.ȱȱ ToȱdetectȱsmallerȱsignificantȱvariationsȱinȱpH,ȱsomeȱknowledgeȱofȱtheȱestuaryȱbeingȱmonitoredȱandȱitsȱ normalȱpHȱvaluesȱisȱrequired.ȱExtremeȱreadingsȱwillȱbeȱimmediatelyȱobvious,ȱhoweverȱsomeȱestuariesȱ mayȱnaturallyȱbeȱlowȱinȱpH,ȱwhileȱotherȱestuaries,ȱpossiblyȱwithȱmoreȱseaȱinfluence,ȱwillȱbeȱ considerablyȱhigher.ȱTheȱnaturalȱpHȱrangeȱofȱmostȱestuarineȱorȱmarineȱareasȱinȱtheȱstateȱshouldȱbeȱ betweenȱ6.5ȱandȱ9.2ȱpHȱunits.ȱValuesȱoutsideȱthisȱrangeȱwouldȱindicateȱsomeȱformȱofȱpastȱorȱpresentȱ humanȱinterferenceȱ What you may need: – siteȱrecordȱformȱ – aȱboxȱofȱpHȱstripsȱthatȱareȱsuitableȱforȱseawater,ȱorȱalternativelyȱaȱmeterȱ – aȱpHȱbufferȱsolutionȱwithȱaȱvalueȱofȱ4ȱorȱ10ȱ – sampleȱdipperȱmountedȱtoȱaȱpoleȱforȱwaterȱsampling.ȱ To ensure good results pHȱstripsȱmayȱbecomeȱinaccurateȱorȱnotȱreactȱatȱallȱasȱtheyȱage,ȱorȱifȱtheyȱareȱexposedȱtoȱwater.ȱIfȱyourȱ packetȱofȱstripsȱbecomesȱwetȱpleaseȱdisposeȱofȱthem.ȱȱ 28 ESTUARINE MONITORING GUIDANCE MANUAL TestȱyourȱpHȱstripsȱbeforeȱeachȱmonitoringȱeventȱtoȱmakeȱsureȱthatȱtheyȱprovideȱanȱaccurateȱreadingȱinȱ aȱreasonableȱlengthȱofȱtime.ȱThisȱisȱdoneȱbyȱimmersingȱoneȱofȱtheȱstripsȱintoȱaȱfreshȱsolutionȱofȱknownȱ pH,ȱcheckingȱthatȱtheȱstripȱprovidesȱtheȱcorrectȱreading,ȱandȱthenȱdisposingȱofȱtheȱusedȱstrip.ȱShouldȱ yourȱtestȱstripȱnotȱperformȱadequately,ȱpleaseȱdisposeȱofȱtheȱentireȱpackȱandȱopenȱaȱnewȱone.ȱ EstuariesȱgenerallyȱhaveȱaȱbasicȱpH,ȱsoȱtheȱmostȱappropriateȱpHȱbufferȱforȱestuarineȱmonitoringȱwouldȱ beȱtheȱpH10ȱbuffer.ȱUsingȱaȱpH7ȱbufferȱisȱnotȱrecommendedȱasȱtheȱunreactedȱcolourȱofȱmostȱpHȱstripsȱisȱ inȱtheȱneutralȱrange.ȱȱ IfȱyouȱareȱusingȱaȱpHȱmeterȱorȱcombinedȱCTPȱmeter,ȱensureȱitȱisȱcalibratedȱpriorȱtoȱgoingȱoutȱintoȱtheȱ field.ȱ What to do: Collectȱaȱwaterȱsampleȱfromȱatȱleastȱaȱmetreȱoutȱfromȱtheȱshoreȱ(preferablyȱfurtherȱout)ȱandȱfromȱ approximatelyȱ200ȱmmȱbelowȱtheȱsurface.ȱȱ PlaceȱaȱpHȱstripȱinȱtheȱwaterȱsampleȱandȱleaveȱitȱinȱtheȱwaterȱuntilȱnoȱfurtherȱcolourȱchangeȱoccursȱ(upȱ toȱfiveȱminutes).ȱ RemoveȱpHȱstripȱfromȱsampleȱandȱimmediatelyȱcompareȱtheȱcoloursȱonȱtheȱstripȱwithȱthoseȱonȱtheȱ packet.ȱȱ Recordȱtheȱreadingȱonȱtheȱsiteȱrecordȱform.ȱȱ Shouldȱyouȱbeȱusingȱaȱmeter,ȱgentlyȱplaceȱtheȱprobesȱintoȱtheȱsampleȱandȱallowȱthemȱtoȱequilibrateȱwithȱ theȱsampleȱtemperature.ȱOnceȱtheȱreadingȱhasȱstabilised,ȱrecordȱitȱonȱtheȱsiteȱrecordȱform.ȱ ȱ ȱ ȱ 29 ESTUARINE MONITORING GUIDANCE MANUAL Measuring water clarity Waterȱclarityȱisȱanȱindirectȱmeasureȱofȱtheȱamountȱofȱsuspendedȱ solidsȱinȱtheȱwater.ȱAsȱtheȱamountȱofȱsuspendedȱsolidsȱincreases,ȱ theȱdepthȱthatȱlightȱcanȱpenetrateȱisȱreduced.ȱThereȱisȱnoȱdirectȱ correlationȱbetweenȱtheȱweightȱofȱsolidsȱpresentȱinȱtheȱwaterȱandȱ waterȱclarity.ȱThisȱisȱdueȱtoȱtheȱeffectsȱofȱparticleȱsize–manyȱsmallerȱ particlesȱreflectȱmoreȱlightȱthanȱaȱfewȱbigȱparticles.ȱȱ Waterȱclarityȱ(measuredȱwithȱaȱSecchiȱdisk)ȱisȱfrequentlyȱusedȱasȱanȱ alternativeȱtoȱturbidityȱmeasurement.ȱHowever,ȱtheȱrelationshipȱ betweenȱturbidityȱ(measuredȱinȱnephelometricȱturbidityȱunitsȱorȱ NTUs)ȱandȱclarityȱ(measuredȱinȱdepth)ȱisȱnotȱperfect.ȱAnȱ approximateȱcorrelationȱisȱgivenȱinȱtheȱdataȱinterpretationȱsection,ȱ asȱthisȱisȱcommonȱpracticeȱinȱclearȱwaters.ȱ Waterȱclarityȱmayȱbeȱnaturallyȱlowȱ(turbid)ȱduringȱorȱjustȱafterȱ flooding.ȱLowerȱwaterȱclarityȱcanȱalsoȱbeȱanȱindicationȱofȱunsoundȱ landȱuseȱmanagementȱinȱtheȱestuary’sȱcatchment.ȱHigherȱlevelsȱofȱ erosionȱinȱtheȱcatchmentȱwillȱresultȱinȱlowerȱwaterȱclarityȱinȱtheȱ estuaryȱforȱaȱlongerȱperiodȱofȱtime.ȱȱ Reducedȱwaterȱclarityȱcanȱaffectȱmarineȱplantȱgrowthȱandȱreducesȱ theȱdepthȱrangeȱandȱextentȱofȱseagrassȱandȱmacrophyteȱbeds.ȱTheȱ fineȱparticlesȱmayȱdeterȱsomeȱfishȱspeciesȱorȱclogȱtheȱfeedingȱ apparatusȱofȱfilterȱfeedingȱanimals.ȱAȱwideȱrangeȱofȱtoxicȱorȱharmfulȱ pollutantsȱcanȱattachȱthemselvesȱtoȱfineȱsediment,ȱsoȱlowȱwaterȱ clarityȱmayȱalsoȱindicateȱhighȱlevelsȱofȱotherȱpollutants.ȱȱ Activity: 3 Core Age: All Time: 2 Why? Water clarity controls the depths at which submerged plants can grow. The turbidity (cloudiness) of water may indicate erosion or algal blooms and is a useful measure for mapping stormwater plumes. EPA guidelines for estuaries suggest that 1 NTU or less is good, while more than 10 NTU is poor. Safety: Secchi disks are deployed in deep water (eg from bridges, jetties, wharfs and boats). CommonȱmethodsȱforȱmeasuringȱwaterȱclarityȱincludeȱtheȱSecchiȱdisk,ȱtheȱturbidityȱmeterȱandȱtheȱ turbidityȱtube.ȱEstuariesȱoftenȱhaveȱveryȱhighȱwaterȱclarityȱreadingsȱcomparedȱtoȱfreshwaterȱriversȱandȱ streams,ȱsoȱSecchiȱdisksȱareȱrecommendedȱforȱestuarineȱareas.ȱTurbidityȱmetersȱareȱavailableȱforȱtheȱ turbidityȱrangesȱfoundȱinȱestuaries,ȱandȱtheyȱgiveȱaȱusefulȱandȱoftenȱmoreȱaccurateȱmeasureȱofȱlightȱ availabilityȱtoȱbenthicȱplants.ȱHowever,ȱtheyȱareȱconsiderablyȱmoreȱcostly,ȱlessȱrobustȱandȱrequireȱ calibrationȱusingȱformazineȱstandards,ȱwhichȱareȱtoxic.ȱTurbidityȱtubesȱareȱnotȱsufficientlyȱsensitiveȱforȱ clearȱmarineȱwaters.ȱ What you may need: – siteȱrecordȱformȱ – Secchiȱdiskȱ(directionsȱforȱmakingȱaȱSecchiȱdiskȱareȱincludedȱinȱAppendixȱ1)ȱ – tapeȱmeasureȱ(ifȱSecchiȱdiskȱcordȱisȱnotȱmarkedȱwithȱmillimetres).ȱȱ To ensure good results: Secchiȱdepthȱreadingsȱshouldȱideallyȱnotȱbeȱtakenȱwithinȱtwoȱhoursȱofȱsunsetȱorȱsunrise,ȱtheyȱshouldȱbeȱ doneȱwithȱaȱclearȱsky,ȱcalmȱwaterȱandȱwithoutȱwearingȱsunglasses.ȱ Youȱmayȱfindȱyouȱobtainȱmoreȱconsistencyȱinȱtheȱreadingsȱifȱyouȱtakeȱthemȱfromȱtheȱshadyȱsideȱofȱtheȱ boatȱorȱjetty.ȱȱ Useȱaȱminimalȱstretchȱnylonȱrope,ȱorȱotherȱshrinkȱresistantȱline,ȱforȱtheȱSecchiȱdisk.ȱCheckȱtheȱlengthȱofȱ theȱropeȱeveryȱsixȱmonthsȱtoȱensureȱthatȱitȱhasȱnotȱstretchedȱorȱshrunk.ȱRinseȱtheȱdiskȱoffȱafterȱuseȱsoȱ thatȱsaltȱdoesȱnotȱbuildȱupȱonȱtheȱcordȱorȱdisk.ȱ 30 ESTUARINE MONITORING GUIDANCE MANUAL What to do: ȱ Figure 2—Taking a Secchi depth reading AȱSecchiȱdiskȱisȱaȱspecialȱdiskȱmarkedȱwithȱblackȱandȱwhiteȱquarters.ȱSlowlyȱlowerȱtheȱSecchiȱdiskȱintoȱ theȱwaterȱuntilȱtheȱblackȱandȱwhiteȱdiskȱjustȱdisappearsȱfromȱsight.ȱNoteȱthisȱdepth.ȱȱ Whenȱtheȱdiskȱdisappears,ȱraiseȱtheȱdiskȱslowlyȱuntilȱitȱjustȱbecomesȱvisibleȱagain.ȱYouȱneedȱtoȱbeȱableȱtoȱ distinguishȱbetweenȱtheȱblackȱandȱwhiteȱsectionsȱofȱtheȱdisk.ȱNoteȱthisȱdepth.ȱ TheȱSecchiȱdepthȱisȱtheȱaverageȱbetweenȱtheȱtwoȱreadings.ȱMostȱSecchiȱdisksȱhaveȱaȱmarkedȱcord,ȱwhichȱ makesȱrecordingȱtheseȱdepthsȱeasy,ȱprovidingȱyouȱareȱnotȱtooȱhighȱaboveȱtheȱwater.ȱIfȱtheȱdiskȱreachesȱ theȱbottomȱofȱtheȱestuaryȱandȱcanȱstillȱbeȱseenȱthenȱrecordȱtheȱdepthȱasȱ‘greaterȱthan’ȱandȱtheȱdepthȱofȱ theȱwaterȱatȱthatȱpoint.ȱ RecordȱtheȱSecchiȱdepth,ȱmeasurementȱsite,ȱviewingȱdistanceȱfromȱobserverȱtoȱwaterȱlineȱandȱanythingȱ elseȱyouȱthinkȱmayȱhaveȱanȱeffectȱonȱtheȱresult,ȱonȱyourȱsiteȱrecordȱform.ȱȱ Theȱconversionȱbetweenȱturbidityȱandȱwaterȱclarityȱvaries,ȱhoweverȱaȱroughȱ‘ruleȱofȱthumb’ȱforȱSouthȱ Australianȱestuariesȱ(developedȱbyȱtheȱEPA)ȱisȱincludedȱbelowȱ(Tableȱ1).ȱȱ Table 1—Secchi depth and turbidity relationship 2ȱNTUȱ 10ȱmȱ 5ȱNTUȱ 4ȱmȱ 10ȱNTUȱ 2ȱmȱ 25ȱNTUȱ 0.9ȱmȱ 100ȱNTUȱ 0.2ȱmȱ 31 ESTUARINE MONITORING GUIDANCE MANUAL Measuring salinity Salinityȱisȱaȱmeasureȱofȱtheȱamountȱofȱdissolvedȱsaltsȱinȱaȱgivenȱunitȱ ofȱwater.ȱTheȱsalinityȱofȱseawaterȱisȱnormallyȱaroundȱ3–4%.ȱEstuariesȱ haveȱaȱsalinityȱgradient,ȱwhichȱmeansȱthatȱtheȱsalinityȱatȱoneȱendȱofȱ theȱestuaryȱwillȱdifferȱfromȱthatȱatȱtheȱother.ȱȱ Eachȱestuaryȱhasȱitsȱownȱsalinityȱregime.ȱHumanȱactivities,ȱsuchȱasȱ usingȱwaterȱforȱirrigationȱorȱstormwaterȱdischarge,ȱhaveȱalteredȱ theseȱsalinityȱregimesȱsignificantlyȱinȱmanyȱestuaries.ȱTakingȱregularȱ salinityȱreadingsȱgivesȱyouȱanȱindicationȱofȱwhatȱkindȱofȱsalinityȱ regimeȱoccursȱinȱyourȱestuaryȱandȱthereforeȱwhatȱyouȱmayȱexpectȱ fromȱotherȱparameters.ȱȱ TheȱsalinityȱrangeȱisȱwideȱinȱSouthȱAustralianȱestuariesȱandȱthisȱ makesȱfindingȱinstrumentsȱtoȱmeasureȱsalinityȱdifficult.ȱOneȱmethodȱ isȱtoȱmeasureȱtheȱchangeȱinȱconductivityȱthatȱoccursȱwhenȱsaltsȱareȱ dissolvedȱinȱwater.ȱCommonȱpocketȱconductivityȱmetersȱhaveȱaȱ rangeȱfromȱfreshwaterȱtoȱaboutȱhalfȱthatȱofȱseawater.ȱSomeȱestuariesȱ inȱSouthȱAustraliaȱhaveȱsalinitiesȱofȱupȱtoȱthreeȱorȱfourȱtimesȱthatȱofȱ seawater.ȱConductivityȱmetersȱthatȱmeasureȱtheseȱsortsȱofȱsalinitiesȱ costȱaȱconsiderableȱamountȱandȱrequireȱregularȱcalibrationȱ specificallyȱforȱtheȱsalinityȱrangesȱthatȱareȱmostȱcommonlyȱ encounteredȱinȱtheȱareaȱwhereȱtheyȱareȱused.ȱFinally,ȱtheȱconversionȱ ofȱconductivityȱtoȱsalinityȱasȱtotalȱdissolvedȱsolidsȱ(TDS)ȱinȱg/L,ȱ althoughȱaȱwellȬknownȱrelationship,ȱisȱnotȱaȱsimpleȱlineȱinȱtheȱrangeȱ ofȱsalinitiesȱfoundȱinȱestuaries.ȱȱ Activity: 3 Core Age: All Time: 2 Why? Salinity is the dissolved salt content of water. Salinity and temperature are the primary controllers of stratification in estuaries. Salinity affects the amount of oxygen that can remain dissolved in water, and has an impact on the flocculation rate of suspended particles. Plants and animals are distributed in estuaries according to their salinity preferences. Safety: If using glass instruments, handle carefully. Keep a sharps container on hand for broken glass. Theȱchangeȱinȱtheȱwayȱlightȱrefractsȱthroughȱsaltyȱwaterȱisȱtheȱbasisȱ forȱanotherȱmethodȱthatȱusesȱaȱrefractometerȱtoȱmeasureȱsalinity.ȱ Refractometersȱareȱbothȱrelativelyȱfragileȱandȱhaveȱaȱveryȱsmallȱscale,ȱ makingȱreadingȱtheseȱinstrumentsȱaȱlittleȱdifficultȱforȱnoviceȱusers.ȱ Theȱuseȱofȱhydrometersȱtoȱmeasureȱspecificȱgravityȱ(whichȱcorrelatesȱwellȱtoȱTDSȱasȱg/L)ȱisȱ recommendedȱinȱthisȱprogram,ȱasȱtheyȱareȱavailableȱinȱaȱwideȱselectionȱofȱsalinityȱranges,ȱareȱ economicalȱandȱcanȱbeȱpurchasedȱwithȱaȱqualityȱcertification.ȱȱ Hydrometersȱconsistȱofȱaȱblownȱglassȱtube,ȱsealedȱatȱbothȱends,ȱwithȱaȱbubbleȱofȱairȱinȱtheȱmiddleȱandȱaȱ weightȱatȱtheȱbottom.ȱHydrometersȱworkȱonȱtheȱprincipleȱthatȱtheȱmoreȱsaltȱtheȱwaterȱcontainsȱtheȱ heavierȱorȱdenserȱitȱbecomesȱandȱtheȱbetterȱitȱisȱatȱfloatingȱthings.ȱThisȱisȱwhyȱitȱisȱeasierȱtoȱswimȱinȱsaltȱ waterȱthanȱinȱfreshwater.ȱ Aȱlitreȱofȱpureȱwaterȱweighsȱapproximatelyȱoneȱkgȱandȱhasȱaȱhydrometerȱreading,ȱorȱspecificȱgravity,ȱofȱ 1.000ȱatȱ15.6°C.ȱIfȱyouȱdissolvedȱ20ȱgramsȱofȱsaltȱintoȱthatȱwater,ȱitsȱvolumeȱwouldȱbeȱsimilarȱ(well,ȱaȱ smidginȱmore),ȱbutȱitȱwouldȱweighȱ20ȱgramsȱmore,ȱandȱtheȱhydrometerȱreadingȱwouldȱgoȱupȱ accordingly.ȱȱ Aȱtableȱhasȱbeenȱincludedȱinȱthisȱsectionȱthatȱallowsȱyouȱtoȱconvertȱtheȱhydrometerȱreadingȱofȱyourȱ estuaryȱwaterȱintoȱTDSȱg/L.ȱAsȱwaterȱchangesȱvolumeȱdependingȱonȱtemperature,ȱallȱhydrometerȱ readingsȱneedȱtoȱbeȱcorrectedȱtoȱreflectȱtheȱtemperatureȱofȱtheȱwaterȱasȱwell,ȱandȱtheȱtableȱincludesȱthisȱ correction.ȱȱ 32 ESTUARINE MONITORING GUIDANCE MANUAL What you may need: – siteȱrecordȱformȱ – hydrometerȱinȱtheȱSGȱrangeȱ1.000–1.050ȱ – samplingȱcontainerȱattachedȱtoȱaȱpoleȱ – thermometer.ȱ To ensure good results: Useȱaȱhydrometerȱthatȱhasȱbeenȱcheckedȱagainstȱaȱknownȱstandardȱ(usuallyȱrecordedȱonȱtheȱboxȱorȱonȱaȱ certificateȱaccompanyingȱtheȱhydrometer.ȱIfȱtheȱhydrometerȱisȱcrackedȱorȱbrokenȱitȱneedsȱtoȱbeȱreplacedȱ withȱaȱnewȱone.ȱTheseȱareȱavailableȱfromȱmostȱscientificȱsupplyȱhouses.ȱȱ Spinningȱtheȱhydrometerȱinȱtheȱwaterȱaȱlittleȱasȱitȱisȱplacedȱintoȱtheȱsampleȱwillȱbreakȱtheȱsurfaceȱtensionȱ ofȱtheȱwaterȱandȱensureȱaȱmoreȱaccurateȱresult.ȱȱ Careȱmustȱbeȱtakenȱthatȱtheȱhydrometerȱisȱsuspendedȱinȱtheȱwaterȱwithoutȱtouchingȱtheȱsidesȱofȱtheȱ samplingȱcontainerȱtoȱensureȱaȱtrueȱresultȱisȱobtained.ȱ Waterȱinȱaȱnarrowȱcontainerȱorȱsurroundingȱaȱverticalȱtubeȱformsȱaȱslightlyȱcurvedȱsurface,ȱcalledȱaȱ ‘meniscus’.ȱTheȱwaterȱlevelȱisȱreadȱatȱtheȱbottomȱofȱtheȱmeniscus.ȱȱ What to do: Collectȱaȱwaterȱsampleȱfromȱatȱleastȱaȱmetreȱoutȱfromȱtheȱ shoreȱ(orȱfurther)ȱandȱapproximatelyȱ200ȱmmȱbelowȱtheȱ surface.ȱȱ Measureȱtheȱtemperatureȱaccordingȱtoȱtheȱwaterȱ temperatureȱguideline.ȱRecordȱthisȱonȱtheȱsiteȱrecordȱ form.ȱȱ Placeȱtheȱhydrometerȱinȱtheȱwaterȱsample.ȱLeaveȱitȱuntilȱ itȱsettles.ȱReadȱoffȱtheȱnumberȱthatȱisȱnearestȱtoȱtheȱbaseȱ ofȱtheȱmeniscusȱ(seeȱFigureȱ3).ȱȱ Recordȱtheȱuncorrectedȱreadingȱonȱdatasheet.ȱMeasureȱ theȱtemperatureȱofȱtheȱwaterȱandȱrecordȱit.ȱTheȱg/LȱTDSȱ mayȱthenȱbeȱcalculatedȱinȱtheȱfield,ȱorȱlater,ȱandȱrecorded.ȱȱ Theȱsalinityȱofȱwaterȱmayȱbeȱstatedȱinȱaȱvarietyȱofȱways.ȱIfȱ tryingȱtoȱcompareȱsalinityȱreadingsȱwithȱtheȱresultsȱofȱ anotherȱgroupȱorȱsurvey,ȱitȱisȱimportantȱthatȱtheȱsalinityȱ unitȱisȱknown.ȱAȱconversionȱchartȱbetweenȱsomeȱofȱtheȱ moreȱcommonȱtypesȱofȱsalinityȱreadingsȱisȱprovidedȱinȱ Tableȱ3.ȱ Figure 3—Reading a hydrometer Shouldȱyouȱbeȱusingȱaȱconductivityȱmeterȱtoȱmeasureȱ salinity,ȱbeȱsureȱtoȱfollowȱtheȱmanufacturer’sȱinstructions.ȱ Manyȱconductivityȱmetersȱandȱprobesȱhaveȱtemperatureȱ compensation,ȱwhichȱautomaticallyȱcompensatesȱtheȱ readingȱtoȱanȱECȱvalueȱatȱ25°C.ȱYouȱwillȱneedȱtoȱreferȱtoȱ theȱowner’sȱmanualȱtoȱfindȱoutȱifȱtheȱmeterȱyouȱareȱusingȱ hasȱthisȱfeature,ȱandȱmakeȱaȱnoteȱonȱtheȱdatasheet.ȱȱ Ifȱthereȱisȱnoȱautomaticȱtemperatureȱcompensationȱonȱyourȱmeter,ȱthenȱyourȱECȱreadingsȱwillȱneedȱtoȱbeȱ modifiedȱusingȱtheȱformulaȱbelow:ȱȱ K25 (conductivity at 25 °C) = conductivity 1 + 0.019 (water temperature — 25) 33 ESTUARINE MONITORING GUIDANCE MANUAL Table 2—Obtaining TDS g/L from SG and temperature 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0 21.0 22.0 23.0 24.0 25.0 26.0 27.0 28.0 29.0 30.0 31.0 32.0 33.0 1.000 0.0 0.2 0.3 0.3 0.3 0.3 0.4 0.4 0.4 0.5 0.6 0.8 1.1 1.4 1.6 1.9 2.1 2.5 2.9 3.2 3.6 4.0 4.4 4.8 5.1 1.001 0.2 0.2 0.2 0.2 0.5 0.5 0.6 0.6 0.7 0.8 1.0 1.2 1.5 1.6 1.9 2.1 2.4 2.5 2.8 3.1 3.4 3.8 4.2 4.5 4.9 5.1 5.5 5.9 6.4 1.002 1.5 1.5 1.6 1.6 1.6 1.8 1.9 2.0 2.1 2.3 2.4 2.5 2.8 2.9 3.2 3.4 3.7 4.0 4.2 4.6 4.9 5.1 5.5 5.9 6.3 6.6 7.0 7.5 7.9 1.003 2.8 2.8 2.8 2.9 2.9 3.1 3.2 3.3 3.4 3.6 3.7 3.8 4.1 4.2 4.5 4.8 5.0 5.3 5.5 5.9 6.2 6.6 6.8 7.2 7.6 8.0 8.4 8.8 9.2 1.004 4.1 4.1 4.1 4.2 4.2 4.4 4.5 4.6 4.8 4.9 5.0 5.1 5.4 5.5 5.8 6.1 6.3 6.6 7.0 7.2 7.5 7.9 8.3 8.5 8.9 9.3 9.7 10.1 10.5 1.005 5.3 5.4 5.4 5.4 5.5 5.5 5.7 5.8 5.9 6.2 6.3 6.6 6.7 7.0 7.1 7.4 7.6 7.9 8.3 8.5 8.9 9.2 9.6 10.0 10.2 10.6 11.1 11.5 11.9 1.006 6.6 6.6 6.7 6.7 6.8 6.8 7.0 7.1 7.2 7.5 7.6 7.9 8.0 8.3 8.5 8.8 9.1 9.3 9.6 9.8 10.2 10.5 10.9 11.3 11.6 12.0 12.4 12.8 13.2 1.007 7.9 7.9 7.9 8.0 8.1 8.1 8.3 8.4 8.5 8.8 8.9 9.2 9.3 9.6 9.8 10.1 10.4 10.6 10.9 11.3 11.5 11.9 12.2 12.6 13.0 13.4 13.8 14.2 14.6 1.008 9.2 9.2 9.2 9.3 9.3 9.4 9.6 9.7 9.8 10.0 10.2 10.5 10.6 10.9 11.1 11.4 11.7 11.9 12.2 12.6 12.8 13.2 13.6 13.9 14.3 14.7 15.1 15.5 15.9 1.009 10.4 10.5 10.5 10.6 10.6 10.7 10.9 11.0 11.1 11.3 11.5 11.8 11.9 12.2 12.4 12.7 13.0 13.2 13.6 13.9 14.1 14.5 14.9 15.3 15.7 16.1 16.5 16.9 17.3 1.010 11.7 11.7 11.8 11.8 11.9 12.0 12.2 12.3 12.4 12.6 12.8 13.1 13.2 13.5 13.7 14.0 14.3 14.5 14.9 15.2 15.6 15.8 16.2 16.6 17.0 17.4 17.8 18.2 18.7 1.011 13.0 13.0 13.1 13.1 13.2 13.4 13.5 13.6 13.7 13.9 14.1 14.4 14.5 14.8 15.0 15.3 15.6 16.0 16.2 16.5 16.9 17.3 17.5 17.9 18.3 18.8 19.2 19.6 20.0 1.012 14.1 14.3 14.3 14.4 14.5 14.7 14.8 14.9 15.0 15.2 15.4 15.7 15.8 16.1 16.3 16.6 17.0 17.3 17.5 17.9 18.2 18.6 19.0 19.3 19.6 20.1 20.5 20.9 21.4 1.013 15.4 15.4 15.6 15.7 15.8 15.8 16.0 16.2 16.3 16.5 16.7 17.0 17.1 17.4 17.7 17.9 18.3 18.6 18.8 19.2 19.5 19.9 20.3 20.6 21.0 21.4 21.9 22.3 22.7 1.014 16.7 16.7 16.9 17.0 17.0 17.1 17.3 17.5 17.7 17.8 18.0 18.3 18.6 18.7 19.0 19.3 19.6 19.9 20.1 20.5 20.9 21.2 21.6 22.0 22.3 22.7 23.2 23.6 24.0 1.015 17.9 18.0 18.0 18.2 18.3 18.4 18.6 18.8 19.0 19.1 19.3 19.6 19.9 20.0 20.4 20.6 20.9 21.2 21.6 21.8 22.2 22.5 22.9 23.3 23.6 24.0 24.6 25.0 25.3 1.016 19.2 19.3 19.3 19.5 19.6 19.7 19.9 20.1 20.3 20.4 20.6 20.9 21.2 21.3 21.7 22.0 22.2 22.5 22.9 23.3 23.5 23.9 24.3 24.7 25.1 25.5 25.9 26.3 26.8 1.017 20.5 20.5 20.6 20.8 20.9 21.0 21.2 21.3 21.6 21.7 22.0 22.2 22.5 22.7 23.0 23.3 23.5 23.8 24.2 24.6 24.8 25.2 25.6 26.0 26.4 26.9 27.3 27.7 28.1 1.018 21.7 21.8 22.0 22.1 22.2 22.3 22.5 22.6 22.9 23.0 23.3 23.5 23.8 24.0 24.3 24.6 24.8 25.2 25.5 25.9 26.1 26.5 26.9 27.3 27.7 28.3 28.7 29.0 29.4 1.019 23.0 23.1 23.3 23.4 23.5 23.6 23.8 23.9 24.2 24.3 24.6 24.8 25.1 25.3 25.6 25.9 26.1 26.5 26.8 27.2 27.6 27.8 28.2 28.6 29.0 29.5 30.0 30.4 30.8 1.020 24.3 24.3 24.4 24.6 24.7 24.8 25.1 25.2 25.5 25.6 25.9 26.1 26.4 26.6 26.9 27.2 27.4 27.8 28.2 28.5 28.9 29.2 29.6 30.0 30.4 30.9 31.2 31.6 32.1 1.021 25.6 25.6 25.7 25.9 26.0 26.1 26.4 26.5 26.8 26.9 27.2 27.4 27.7 27.9 28.2 28.5 28.9 29.1 29.5 29.8 30.2 30.6 30.9 31.3 31.7 32.2 32.5 33.0 33.4 1.022 26.8 26.9 27.0 27.2 27.3 27.4 27.7 27.8 28.1 28.2 28.5 28.7 29.0 29.2 29.5 29.8 30.2 30.4 30.8 31.2 31.5 31.9 32.2 32.6 33.0 33.5 33.9 34.3 34.8 1.023 28.1 28.2 28.3 28.5 28.6 28.7 28.9 29.1 29.4 29.5 29.8 30.0 30.3 30.6 30.8 31.2 31.5 31.7 32.1 32.5 32.8 33.2 33.5 33.9 34.4 34.9 35.2 35.6 36.2 1.024 29.4 29.5 29.5 29.8 29.9 30.0 30.2 30.4 30.6 30.8 31.1 31.3 31.6 31.9 32.1 32.5 32.8 33.2 33.4 33.8 34.2 34.5 35.0 35.4 35.7 36.2 36.5 37.1 37.5 1.025 30.6 30.7 30.8 30.9 31.1 31.3 31.5 31.7 31.9 32.1 32.4 32.6 32.9 33.2 33.4 33.8 34.1 34.5 34.7 35.1 35.5 35.9 36.3 36.7 37.1 37.5 37.8 38.4 38.8 1.026 31.9 32.0 32.1 32.2 32.4 32.6 32.8 33.0 33.2 33.4 33.7 33.9 34.2 34.5 34.7 35.1 35.4 35.8 36.0 36.4 36.8 37.2 37.6 38.0 38.4 38.8 39.3 39.7 40.2 1.027 33.2 33.3 33.4 33.5 33.7 33.9 34.1 34.3 34.5 34.7 35.0 35.2 35.5 35.8 36.2 36.4 36.7 37.1 37.5 37.7 38.1 38.5 38.9 39.3 39.8 40.2 40.6 41.0 41.5 1.028 34.5 34.5 34.7 34.8 35.0 35.1 35.4 35.6 35.8 36.0 36.3 36.5 36.8 37.1 37.5 37.7 38.1 38.4 38.8 39.1 39.4 39.9 40.2 40.7 41.1 41.5 41.9 42.5 42.9 1.029 35.6 35.8 35.9 36.2 36.3 36.4 36.7 36.8 37.1 37.3 37.6 37.8 38.1 38.4 38.8 39.0 39.4 39.7 40.1 40.5 40.8 41.2 41.6 42.0 42.4 42.8 43.3 43.8 44.2 1.030 36.9 37.1 37.2 37.3 37.6 37.7 38.0 38.1 38.4 38.6 38.9 39.1 39.4 39.7 40.1 40.3 40.7 41.0 41.4 41.7 42.1 42.6 42.9 43.3 43.8 44.2 44.6 45.1 45.6 1.031 38.2 38.4 38.5 38.6 38.9 39.0 39.3 39.4 39.7 39.9 40.2 40.5 40.7 41.0 41.4 41.8 42.0 42.3 42.7 43.1 43.4 43.9 44.3 44.7 45.1 45.5 45.9 46.5 46.9 1.032 39.5 39.6 39.8 39.9 40.1 40.3 40.6 40.7 41.0 41.2 41.5 41.7 42.0 42.4 42.7 43.0 43.3 43.7 44.1 44.4 44.8 45.2 45.6 46.0 46.5 46.8 47.3 47.8 48.3 1.033 40.8 40.9 41.0 41.2 41.4 41.6 41.9 42.0 42.3 42.5 42.8 43.0 43.3 43.7 44.1 44.3 44.7 45.0 45.4 45.7 46.2 46.6 46.9 47.3 47.8 48.2 48.6 49.2 49.6 1.034 42.0 42.2 42.3 42.4 42.7 42.9 43.2 43.3 43.6 43.8 44.1 44.3 44.6 45.0 45.4 45.6 46.0 46.3 46.7 47.0 47.5 47.9 48.3 48.7 49.2 49.5 50.0 50.5 51.0 1.035 43.3 43.4 43.6 43.8 44.0 44.1 44.4 44.6 44.9 45.1 45.4 45.7 46.0 46.3 46.7 47.0 47.3 47.6 48.0 48.4 48.8 49.2 49.6 50.0 50.5 50.9 51.3 51.9 52.3 1.036 44.5 44.7 44.8 45.0 45.3 45.4 45.7 45.9 46.2 46.4 46.7 47.0 47.3 47.6 48.0 48.3 48.6 49.0 49.4 49.7 50.1 50.6 50.9 51.4 51.8 52.3 52.7 53.2 53.7 1.037 45.8 46.0 46.1 46.3 46.6 46.7 47.0 47.2 47.5 47.7 48.1 48.3 48.6 48.9 49.4 49.6 49.9 50.3 50.7 51.0 51.5 51.9 52.3 52.7 53.2 53.6 54.0 54.6 55.0 1.038 47.1 47.2 47.4 47.6 47.8 48.0 48.3 48.5 48.8 49.0 49.4 49.6 49.9 50.2 50.7 50.9 51.3 51.6 52.0 52.3 52.8 53.2 53.6 54.0 54.5 54.9 55.4 55.9 56.4 1.039 48.3 48.5 48.7 48.9 49.1 49.3 49.6 49.8 50.1 50.3 50.7 50.9 51.2 51.6 52.0 52.2 52.6 52.9 53.3 53.7 54.1 54.6 54.9 55.4 55.9 56.3 56.7 57.2 57.7 1.040 49.6 49.8 49.9 50.1 50.4 50.5 50.9 51.1 51.4 51.6 52.0 52.2 52.5 52.9 53.3 53.5 53.9 54.3 54.7 55.0 55.5 55.9 56.3 56.7 57.2 57.6 58.0 58.6 59.0 1.041 50.9 51.0 51.2 51.4 51.7 51.8 52.2 52.3 52.7 52.9 53.3 53.5 53.8 54.2 54.7 54.9 55.2 55.6 56.0 56.3 56.8 57.2 57.6 58.0 58.5 59.0 59.4 59.9 60.4 1.042 52.1 52.3 52.5 52.7 53.0 53.1 53.5 53.6 54.0 54.2 54.6 54.8 55.1 55.5 56.0 56.2 56.5 56.9 57.3 57.6 58.1 58.6 58.9 59.4 59.9 60.3 60.7 61.3 61.7 1.043 53.4 53.6 53.8 54.0 54.2 54.4 54.7 54.9 55.3 55.5 55.9 56.1 56.4 56.8 57.3 57.5 57.8 58.2 58.6 59.0 59.4 59.9 60.3 60.7 61.2 61.6 62.1 62.6 63.1 1.044 54.7 54.9 55.0 55.3 55.5 55.7 56.0 56.2 56.6 56.8 57.2 57.4 57.7 58.1 58.6 58.8 59.2 59.5 60.0 60.3 60.8 61.2 61.6 62.1 62.6 63.0 63.4 64.0 64.4 1.045 55.9 56.1 56.3 56.5 56.8 57.0 57.3 57.5 57.9 58.1 58.5 58.7 59.1 59.4 60.0 60.1 60.5 60.9 61.3 61.6 62.1 62.6 62.9 63.4 63.9 64.3 64.8 65.3 65.8 1.046 57.2 57.4 57.6 57.8 58.1 58.2 58.6 58.8 59.2 59.4 59.8 60.0 60.4 60.7 61.3 61.4 61.8 62.2 62.6 62.9 63.4 63.9 64.3 64.7 65.2 65.7 66.1 66.7 67.1 1.047 58.5 58.7 58.8 59.1 59.4 59.5 59.9 60.1 60.5 60.7 61.1 61.3 61.7 62.1 62.6 62.8 63.1 63.5 63.9 64.3 64.8 65.2 65.6 66.1 66.6 67.0 67.4 68.0 68.5 1.048 59.7 59.9 60.1 60.4 60.7 60.8 61.2 61.4 61.8 62.0 62.4 62.6 63.0 63.4 63.9 64.1 64.4 64.8 65.3 65.6 66.1 66.6 66.9 67.4 67.9 68.3 68.8 69.4 69.8 1.049 61.0 61.2 61.4 61.6 61.9 62.1 62.5 62.7 63.1 63.3 63.7 63.9 64.3 64.7 65.3 65.4 65.8 66.2 66.6 66.9 67.4 67.9 68.3 68.7 69.3 69.7 70.1 70.7 71.2 1.050 62.2 62.5 62.7 62.9 63.2 63.4 63.8 64.0 64.4 64.6 65.0 65.2 65.6 66.0 66.6 66.7 67.1 67.5 67.9 68.2 68.8 69.2 69.6 70.1 70.6 71.0 71.5 72.1 72.5 34 ESTUARINE MONITORING GUIDANCE MANUAL TheȱtableȱbelowȱisȱbasedȱonȱoneȱdevelopedȱbyȱProfessorȱBillȱWilliamsȱofȱAdelaideȱUniversity,ȱandȱ presentedȱatȱtheȱ6thȱInternationalȱSymposiumȱonȱSalineȱLakeȱResearch,ȱBeijing,ȱ1994.ȱItȱisȱforȱbrineȱ betweenȱ22°Cȱandȱ25°C.ȱTheȱtableȱmayȱbeȱusedȱtoȱconvertȱbetweenȱvariousȱsalinityȱmeasurements.ȱ Table 3—William's salinity conversion table Specific gravity Brinometer degrees Baume degrees % salinity (% NaCl in brine) Seawater salinity % scale TDS (g/L or ppt w/v) TDS (g/kg or ppt w/w) EC (mS/cm) 1.0000 0 0.0 0.000 0 0.0 0 0.0 1.0040 2 0.6 0.528 16 6.6 6 9.0 1.0050 3 0.7 0.704 20 8.2 7 11.0 1.0070 4 1.0 1.056 29 10.9 10 16.0 1.0100 5 1.4 1.452 40 14.9 14 21.8 1.0107 6 1.5 1.544 43 15.1 15 23.2 1.0110 6 1.6 1.584 44 15.5 15 23.8 1.0140 8 2.0 2.020 57 20.2 20 30.2 1.0150 8 2.1 2.112 60 21.8 21 31.6 1.0180 9 2.5 2.482 71 25.4 25 37.1 1.0190 10 2.7 2.640 76 27.1 27 39.0 1.0200 10 2.8 2.772 80 28.6 28 41.0 1.0214 11 3.0 2.956 86 30.6 30 44.0 1.0230 12 3.3 3.167 92 32.9 32 47.0 1.0250 13 3.5 3.540 100 35.8 35 50.7 1.0260 14 3.7 3.695 37.3 36 52.5 1.0286 15 4.0 4.038 41.1 40 57.3 1.0300 16 4.2 4.223 42.4 42 59.8 1.0322 17 4.5 4.513 46.4 45 63.8 1.0340 18 4.8 4.750 49.0 47 66.9 1.0359 19 5.0 5.001 51.7 50 70.1 1.0380 20 5.3 5.279 54.1 53 73.7 1.0395 21 5.5 5.477 55.8 55 76.3 1.0400 21 5.6 5.543 56.4 56 77.0 1.0420 22 5.8 5.807 60.3 58 81.0 1.0431 23 6.0 5.952 62.5 60 82.4 1.0460 24 6.4 6.335 66.7 64 87.0 1.0468 24 6.5 6.441 67.9 65 88.3 1.0500 26 6.9 6.863 70.6 ȱ ȱ 35 ESTUARINE MONITORING GUIDANCE MANUAL Mapping your estuary Understandingȱtheȱmixingȱprocessesȱinȱyourȱestuaryȱrequiresȱanȱ understandingȱofȱtheȱgeographyȱofȱtheȱestuary.ȱSaltwaterȱandȱ freshwaterȱmixȱdifferentlyȱdependingȱonȱtheȱshapeȱofȱtheȱ‘container’ȱ theyȱareȱin.ȱȱ Toȱdemonstrateȱthis,ȱmixingȱpatternsȱinȱaȱ250–mLȱmeasuringȱ cylinderȱmayȱbeȱcomparedȱwithȱmixingȱpatternsȱinȱaȱwideȱshallowȱ bowl.ȱSimplyȱpourȱ100–mLȱofȱthickȱoilȱintoȱtheȱtwoȱcontainers,ȱthenȱ 100–mLȱofȱveryȱthinȱoilȱonȱtop.ȱGentleȱswirlingȱofȱtheȱshallowȱbowlȱ willȱcauseȱconsiderableȱmixing,ȱwhileȱgentleȱswirlingȱofȱtheȱdeep,ȱ narrowȱmeasuringȱcylinderȱwillȱhaveȱlittleȱeffectȱatȱall.ȱ Examinationȱofȱmapsȱofȱyourȱestuaryȱmayȱhelpȱtoȱexplainȱsomeȱofȱ theȱreadingsȱyouȱobtainȱinȱtheȱfield.ȱMappingȱalsoȱallowsȱyouȱtoȱ measureȱshorelineȱchangesȱsuchȱasȱerosion,ȱorȱtheȱdevelopmentȱofȱ sandbanksȱthatȱmayȱchangeȱflowȱpatternsȱwithinȱtheȱestuary.ȱ Aȱwideȱvarietyȱofȱmapsȱisȱavailable.ȱStandardȱreferencesȱforȱyourȱ estuaryȱshouldȱincludeȱatȱleastȱoneȱlarge–scaleȱtopographicȱmap.ȱAsȱ youȱdeepenȱyourȱunderstandingȱofȱyourȱestuary,ȱyouȱmayȱwishȱtoȱ addȱbathymetricȱmapsȱ(thatȱmapȱtheȱunderwaterȱfeatures),ȱaerialȱ photography,ȱorthophotoȱmapsȱ(aerialȱphotographsȱthatȱhaveȱbeenȱ georectifiedȱandȱhaveȱtopographicȱdetailsȱsuperimposedȱonȱthem),ȱ andȱsatelliteȱimagery.ȱȱ Activity: Extension Age: High school Time: Variable Why? The width-to-lengthto-depth ratio of an estuary has an influence on the mixing of the fresh and saline waters within it. Mapping shoreline changes over time may allow any changes in the quantity of flow, or the effects of riparian clearing, to be noted. Safety: This is a desk-based activity. Mapsȱmayȱbeȱpurchasedȱasȱpaperȱproducts,ȱorȱdigitally,ȱonȱCDȱROM.ȱInȱSouthȱAustralia,ȱMaplandȱ providesȱaȱwideȱrangeȱofȱmappingȱproductsȱforȱareasȱwithinȱtheȱstate,ȱandȱaȱsmallerȱrangeȱforȱotherȱ partsȱofȱAustralia.ȱ What you may need: – largeȱscaleȱmapsȱorȱaerialȱphotosȱofȱyourȱestuaryȱ(tryȱtoȱgetȱaȱmapȱthatȱincludesȱyourȱestuaryȱonȱ oneȱsheet—ifȱyouȱhaveȱaȱreallyȱlargeȱestuary,ȱchooseȱtheȱscaleȱthatȱshowsȱyouȱtheȱmostȱdetail,ȱ andȱpasteȱasȱmanyȱmapsȱtogetherȱasȱyouȱrequire)ȱ – tideȱbooksȱ – measuringȱtoolsȱsuchȱasȱcallipers,ȱrulersȱandȱcompasses.ȱ What to do: Ifȱyouȱneedȱhelpȱinterpretingȱtopographicȱmaps,ȱMaplandȱstockȱaȱrangeȱofȱbooksȱthatȱmayȱassist.ȱTwoȱ Australianȱbooksȱonȱmapȱreadingȱare:ȱ Armstrong,ȱPȱ(1987),ȱReadingȱandȱInterpretationȱofȱAustralianȱandȱNewȱZealandȱMaps,ȱ2ndȱedition,ȱ LongmanȱCheshire,ȱMelbourne.ȱ StateȱEmergencyȱServiceȱ(1991),ȱMapȱReadingȱHandbook,ȱ2ndȱedition,ȱTASMAP,ȱDeptȱofȱEnvironmentȱ andȱPlanning,ȱTasmania.ȱ Usingȱtheȱscaleȱonȱyourȱmapȱasȱaȱguide,ȱworkȱoutȱhowȱlongȱyourȱestuaryȱis.ȱItȱmayȱbeȱtrickyȱ determiningȱwhereȱtheȱheadȱofȱtheȱestuaryȱis—itȱisȱusuallyȱconsideredȱtoȱbeȱtheȱpointȱfarthestȱupstreamȱ thatȱstillȱhasȱaȱriseȱandȱfallȱinȱtide.ȱThisȱmayȱnotȱbeȱmarkedȱonȱyourȱmapȱandȱyouȱmayȱneedȱtoȱmakeȱ someȱfieldȱobservations.ȱ Takeȱseveralȱmeasurementsȱacrossȱtheȱwidthȱofȱtheȱestuary.ȱWhatȱisȱtheȱaverageȱwidthȱofȱyourȱestuary?ȱ Doesȱitȱvaryȱmuch?ȱAȱveryȱwideȱestuaryȱmayȱsometimesȱbeȱsubjectȱtoȱCoriolisȱforces,ȱwhichȱpushȱtheȱ 36 ESTUARINE MONITORING GUIDANCE MANUAL denserȱseawaterȱtoȱoneȱsideȱofȱtheȱestuary,ȱandȱtheȱfreshwaterȱtoȱtheȱother.ȱDoesȱthisȱhappenȱinȱyourȱ estuary?ȱ Nowȱtryȱtoȱcalculateȱtheȱareaȱofȱyourȱestuary.ȱYouȱwillȱneedȱthisȱinformationȱtoȱdetermineȱwhetherȱyourȱ estuaryȱisȱnegativeȱorȱpositiveȱ(seeȱtheȱguidelineȱIsȱyourȱestuaryȱpositiveȱorȱnegative?).ȱMeasuringȱareaȱonȱ mapsȱthatȱhaveȱaȱgridȱdrawnȱonȱthemȱisȱnotȱdifficult,ȱbutȱitȱmayȱbeȱharderȱonȱaerialȱphotographs.ȱSeeȱtheȱ guidelineȱUsingȱaerialȱphotographsȱtoȱmapȱhabitatȱchangeȱforȱdetailsȱonȱworkingȱoutȱareas.ȱ Depthȱisȱanȱimportantȱmeasure.ȱItȱmayȱbeȱavailableȱfromȱbathymetricȱmaps,ȱorȱfromȱyourȱharbourȱ master.ȱIsȱyourȱestuaryȱdeepȱwithȱsteepȱsides,ȱorȱwideȱandȱshallowȱwithȱdeeperȱchannels?ȱ Onceȱyouȱknowȱtheȱaverageȱdepth,ȱyouȱcanȱcalculateȱtheȱvolumeȱofȱwaterȱinȱyourȱestuary.ȱForȱaȱveryȱ roughȱestimate,ȱmultiplyȱtheȱsquareȱmetresȱofȱareaȱbyȱtheȱmetresȱofȱdepth.ȱEachȱresultingȱcubicȱmetreȱisȱ aȱkilolitreȱofȱwater.ȱToȱgetȱaȱmoreȱaccurateȱmeasure,ȱdivideȱyourȱestuaryȱupȱintoȱdifferentȱzones,ȱandȱ calculateȱtheȱareaȱandȱvolumeȱforȱeachȱzone.ȱ Eachȱdayȱtheȱtideȱcomesȱinȱandȱoutȱofȱyourȱestuary.ȱWhenȱtheȱmoonȱisȱfull,ȱtheȱspringȱtidesȱbringȱinȱ hugeȱamountsȱofȱwater,ȱwhileȱtheȱneapȱtidesȱofȱhalfȬmoonȱhaveȱtheȱsmallestȱtidalȱexchange.ȱByȱlookingȱ atȱtheȱdifferenceȱinȱheightȱbetweenȱhighȱandȱlowȱtideȱatȱneapsȱandȱsprings,ȱandȱthenȱlookingȱatȱyourȱ topographicȱandȱbathymetricȱmaps,ȱyouȱmayȱbeȱableȱtoȱgainȱaȱroughȱideaȱofȱtheȱvolumeȱofȱwaterȱthatȱ movesȱinȱandȱoutȱwithȱtheȱtide.ȱThisȱwater,ȱtheȱtidalȱexchange,ȱhasȱaȱmajorȱinfluenceȱofȱhowȱlongȱitȱ takesȱforȱpollutantsȱtoȱdisperseȱinȱyourȱestuary.ȱAnȱestuaryȱwithȱaȱsmallȱtidalȱexchangeȱretainsȱtheȱ pollutantsȱforȱconsiderablyȱlongerȱthanȱaȱsimilarȱestuaryȱwithȱmoreȱexchange.ȱ Youȱmayȱgainȱquiteȱaȱlotȱofȱinformationȱaboutȱshorelineȱdeposits,ȱerosionalȱareasȱandȱsandȱbarsȱfromȱ yourȱmaps.ȱBesidesȱusingȱthatȱinformationȱinȱyourȱfieldwork,ȱnoteȱanyȱchangesȱyouȱobserve.ȱLargeȱ changesȱinȱtheȱshorelineȱorȱinȱdepositionȱareasȱsuchȱasȱsandbanksȱmayȱreflectȱlandȱmanagementȱ practicesȱinȱtheȱcatchment.ȱ Theȱinformationȱthatȱyouȱgatherȱinȱthisȱactivityȱwillȱbeȱveryȱusefulȱinȱtheȱactivitiesȱthatȱfollowȱinȱtheȱnextȱ fewȱpages.ȱ Ifȱyourȱgroupȱbecomesȱveryȱinterestedȱinȱmapping,ȱyouȱmayȱbeȱpreparedȱtoȱspendȱtheȱtimeȱtoȱlearnȱtheȱ useȱofȱaȱcomputerisedȱgeographicȱinformationȱsystemȱ(GIS).ȱTheseȱpackagesȱsimplifyȱtheȱ georectificationȱandȱscalingȱofȱaerialȱphotographs,ȱandȱallowȱyouȱtoȱoverlayȱyourȱfieldȱmeasurementsȱ ontoȱtopographicȱandȱotherȱbaseȱmaps.ȱTheyȱprovideȱaȱveryȱsophisticatedȱanalysis.ȱFreeȱpublicȱdomainȱ GISȱpackagesȱareȱavailableȱforȱthoseȱinterestedȱinȱexploringȱthisȱtechnology.ȱMoreȱinformationȱ(andȱtheȱ software)ȱcanȱbeȱfoundȱatȱtheȱfollowingȱwebȱaddresses:ȱ —ȱ <www.microimages.com>ȱisȱtheȱsiteȱforȱfreeȱTNTliteȱsoftwareȱ(sizeȱlimitedȱversionȱofȱTNTmips)ȱ —ȱ <grass.baylor.edu>ȱisȱtheȱsiteȱforȱfree,ȱopenȱsourceȱGRASSȱsoftwareȱdevelopedȱbyȱtheȱUSȱmilitaryȱ —ȱ <http://www.esri.com/software/arcexplorer/>ȱisȱaȱveryȱbasicȱfreeȱGISȱfromȱtheȱmakersȱofȱArcInfoȱ —ȱ <www.tiem.utk.edu/~sada/>ȱisȱtheȱfreeȱSpatialȱAnalysisȱandȱDecisionȱAssistanceȱsoftwareȱpageȱ —ȱ <www.mapmaker.com>ȱisȱtheȱsiteȱtoȱdownloadȱMapmakerȱGratis.ȱ ȱ ȱ 37 ESTUARINE MONITORING GUIDANCE MANUAL Is your estuary positive or negative? Positiveȱestuariesȱareȱthoseȱwhereȱtheȱamountȱofȱfreshwaterȱthatȱ naturallyȱentersȱtheȱestuaryȱexceedsȱtheȱamountȱofȱfreshwaterȱlostȱtoȱ theȱairȱasȱevaporationȱ(seeȱFigureȱ4).ȱSuchȱanȱestuaryȱwillȱhaveȱaȱ salinityȱgradientȱthatȱisȱfreshȱatȱitsȱheadȱandȱwillȱgraduallyȱincreaseȱ toȱmarineȱatȱitsȱmouth.ȱ Inȱcontrast,ȱaȱnegativeȱestuaryȱlosesȱmoreȱfreshwaterȱtoȱevaporationȱ eachȱdayȱthanȱentersȱtheȱestuary.ȱThisȱtypeȱofȱestuaryȱwillȱhaveȱ hypersalineȱ(veryȱsaltyȱwater)ȱatȱitsȱhead,ȱandȱtheȱsalinityȱdecreasesȱ toȱthatȱofȱseawaterȱatȱtheȱmouth.ȱNegativeȱestuariesȱareȱveryȱslowȱatȱ dispersingȱpollutants–andȱthisȱmayȱbeȱevenȱmoreȱnoticeableȱifȱtidalȱ exchangeȱisȱlow.ȱ Activity: Extension Age: High school Time: 3 Why? This factor determines the types of salinity patterns in your estuary. Safety: This is a desk-based activity. InȱSouthȱAustraliaȱmanyȱofȱourȱestuariesȱareȱnegative.ȱThisȱisȱtheȱ resultȱofȱaȱcombinationȱofȱfactors:ȱtheȱoverallȱaridityȱ(highȱ evaporationȱandȱlowȱrainfall),ȱandȱtheȱsizeȱofȱourȱriverȱcatchments.ȱ Onlyȱoneȱofȱourȱriversȱ(theȱMurray)ȱhasȱaȱcatchmentȱthatȱextendsȱintoȱ wetterȱpartsȱofȱAustralia.ȱ What you may need: – theȱareaȱofȱyourȱestuaryȱ – theȱgrossȱannualȱevaporationȱforȱyourȱdistrictȱ – theȱannualȱrainfallȱforȱyourȱdistrictȱ – aȱtopographicȱmapȱorȱcatchmentȱmapȱforȱyourȱestuaryȱ – alternatively,ȱgaugingȱstationȱdataȱforȱtheȱriverȱupstreamȱfromȱtheȱestuaryȱcanȱbeȱusedȱtoȱ determineȱwaterȱinputȱfromȱupstream.ȱ ȱ Figure 4—Water movements in an estuary What to do: Forȱaȱsmallȱcatchment:ȱTakeȱyourȱtopographicȱorȱcatchmentȱmapȱandȱdetermineȱwhatȱcatchmentȱareaȱisȱ coveredȱwithȱurbanȱdevelopmentȱandȱwhatȱareaȱisȱvegetatedȱorȱfarmed.ȱRecordȱtheseȱareasȱasȱ‘urbanȱ area’ȱandȱ‘ruralȱarea’.ȱȱ 38 ESTUARINE MONITORING GUIDANCE MANUAL Toȱworkȱoutȱtheȱvolumeȱofȱrainȱthatȱmayȱrunȱoffȱintoȱtheȱriverȱ(andȱestuary)ȱeachȱyearȱfromȱtheȱland,ȱ multiplyȱeachȱareaȱ(inȱm2)ȱbyȱtheȱannualȱrainfallȱ(inȱmetres),ȱthenȱbyȱaȱrunoffȱcoefficient.ȱWhileȱveryȱ accurateȱrunoffȱcoefficientsȱhaveȱbeenȱdevelopedȱforȱallȱtypesȱofȱdevelopment,ȱforȱthisȱpurposeȱtwoȱveryȱ approximateȱfactorsȱareȱused.ȱTheȱrunoffȱcoefficientȱforȱurbanȱareasȱinȱthisȱcalculationȱisȱ0.65,ȱandȱtheȱ runoffȱcoefficientȱforȱruralȱareasȱisȱ0.12.ȱRecordȱtheȱcubicȱmetresȱofȱwaterȱthatȱrunȱintoȱyourȱestuaryȱforȱ theȱyearȱasȱinputȱvolume.ȱEachȱcubicȱmetreȱisȱequalȱtoȱaȱkilolitreȱofȱwater,ȱandȱ1000ȱcubicȱmetresȱequalȱaȱ megalitre.ȱ Nowȱcalculateȱtheȱamountȱofȱwaterȱthatȱwillȱevaporateȱfromȱtheȱwaterȱsurfaceȱofȱyourȱestuary.ȱForȱthisȱ youȱneedȱtoȱobtainȱtheȱevaporationȱdataȱforȱyourȱareaȱfromȱtheȱBOMȱwebsite.ȱTheȱevaporationȱthatȱtheȱ BOMȱprovidesȱisȱusuallyȱinȱtheȱformȱofȱgrossȱevaporation,ȱratherȱthanȱpanȱorȱnetȱevaporation.ȱSubtractȱ theȱannualȱrainfallȱ(inȱmm)ȱfromȱtheȱgrossȱevaporationȱ(inȱmm)ȱtoȱobtainȱpanȱevaporation.ȱConvertȱthisȱ figureȱfromȱmillimetresȱtoȱmetres,ȱthenȱmultiplyȱitȱbyȱtheȱareaȱofȱyourȱestuaryȱ(inȱm2).ȱRecordȱtheȱresultȱ (inȱcubicȱmetres)ȱasȱevaporationȱvolume.ȱ Whichȱofȱtheseȱfiguresȱisȱtheȱlarger?ȱIfȱtheȱinputȱvolumeȱisȱlargest,ȱyourȱestuaryȱisȱaȱpositiveȱestuary.ȱIfȱ theȱevaporationȱvolumeȱisȱtheȱlarger,ȱyourȱestuaryȱisȱaȱnegativeȱestuary.ȱ Forȱaȱlargeȱcatchment:ȱInȱriversȱsuchȱasȱtheȱMurray,ȱthereȱareȱmanyȱsubȬcatchmentsȱthatȱcoverȱhugeȱ areasȱthatȱhaveȱdifferentȱannualȱrainfallȱandȱevaporation.ȱIfȱyourȱestuaryȱisȱthisȱtypeȱofȱestuary,ȱyouȱcanȱ stillȱcalculateȱtheȱevaporationȱvolumeȱasȱspecifiedȱabove.ȱ Toȱcalculateȱtheȱinputȱvolume,ȱyouȱwillȱrequireȱgaugingȱdataȱfromȱweirsȱonȱyourȱriver,ȱasȱcloseȱtoȱtheȱ estuaryȱasȱpossible.ȱThisȱwillȱtellȱyouȱtheȱvolumeȱofȱwaterȱthatȱpassedȱoverȱtheȱweirȱinȱaȱyear.ȱTheȱdataȱ mayȱbeȱinȱkilolitresȱorȱmegalitres.ȱȱ Trapsȱforȱtheȱunwary:ȱInterȬbasinȱwaterȱtransferȱ(forȱexample,ȱfromȱtheȱRiverȱMurrayȱtoȱtheȱTorrensȱandȱ Onkaparingaȱrivers)ȱmayȱprovideȱanȱunnaturalȱsourceȱofȱfreshwaterȱtoȱyourȱestuary.ȱIfȱyourȱcalculationsȱ basedȱonȱrunoffȱshowȱthatȱyourȱestuaryȱwouldȱnormallyȱbeȱaȱnegativeȱestuary,ȱbutȱyourȱfieldȱdataȱ showsȱitȱtoȱbeȱaȱpositiveȱestuary,ȱyouȱmayȱneedȱtoȱconsiderȱwhereȱtheȱextraȱfreshwaterȱisȱcomingȱfrom.ȱ InterȬbasinȱtransfersȱareȱnotȱalwaysȱobvious.ȱForȱexample,ȱtheȱoutfallȱfromȱaȱsewageȱworksȱisȱmainlyȱ waterȱthatȱoriginallyȱenteredȱyourȱhouseȱasȱmainsȱwater.ȱAreȱyouȱonȱaȱpipedȱwaterȱscheme?ȱManyȱ townshipsȱinȱSAȱobtainȱtheirȱpipedȱwaterȱfromȱtheȱRiverȱMurray.ȱ Reuse:ȱPasturesȱandȱforestsȱhaveȱmuchȱlowerȱrunoffȱratesȱthanȱurbanȱareas,ȱwhichȱhaveȱsealedȱroadsȱ andȱroofs.ȱWhatȱ(approximately)ȱwouldȱbeȱtheȱrunoffȱfromȱyourȱcatchment,ȱifȱthereȱwereȱnoȱurbanȱ areas?ȱIsȱitȱveryȱdifferentȱfromȱtheȱcurrentȱrunoff?ȱȱ Theȱcalculationsȱaboveȱareȱextremelyȱsimplifiedȱformsȱofȱtheȱcomplexȱmodelsȱthatȱnaturalȱresourceȱ managementȱ(NRM)ȱboardsȱmayȱuseȱtoȱdetermineȱhowȱmuchȱurbanȱstormwaterȱrunoffȱtheyȱmayȱ responsiblyȱharvestȱandȱstoreȱinȱundergroundȱaquifersȱforȱreuse.ȱAreȱthereȱanyȱaquiferȱstorageȱprojectsȱ inȱyourȱarea?ȱHowȱmuchȱwaterȱisȱbeingȱinjectedȱintoȱtheȱaquiferȱinȱthoseȱprojects?ȱDon’tȱforgetȱtoȱ subtractȱthatȱamountȱfromȱyourȱinputȱvolume!ȱ ȱ ȱ 39 ESTUARINE MONITORING GUIDANCE MANUAL Classifying your estuary GeoscienceȱAustraliaȱhasȱproducedȱaȱCDȱROMȱaboutȱAustralianȱ estuaries,ȱdesignedȱforȱeducationalȱuse,ȱthatȱincludesȱaȱclassificationȱ systemȱforȱestuariesȱ(commonlyȱcalledȱtheȱOzEstuariesȱ classification,ȱdevelopedȱbyȱHeapȱetȱal,ȱ2001).ȱTheȱsystemȱclassifiesȱ estuariesȱintoȱthreeȱmajorȱtypes,ȱdependingȱonȱwhetherȱtheyȱareȱ dominatedȱbyȱtheȱtideȱ(mainlyȱfoundȱinȱnorthernȱAustraliaȱwhereȱ tidesȱareȱlarge),ȱbyȱriversȱ(theseȱareȱdeltaȱestuaries)ȱorȱbyȱwavesȱ (whereȱaȱsandyȱbarrierȱofȱsomeȱkindȱisȱformedȱbyȱwaves).ȱEachȱofȱ theseȱgroupsȱcanȱbeȱsubdivided,ȱprovidingȱsixȱsubclasses:ȱtideȱ dominatedȱdeltas,ȱwaveȱdominatedȱdeltas,ȱwaveȱdominatedȱ estuaries,ȱstrandȱplains,ȱtideȱdominatedȱestuariesȱandȱtidalȱflats.ȱȱ What you may need: – GeoscienceȱAustraliaȱ(2002)ȱAustralianȱEstuariesȱandȱCoastalȱ Waterways,ȱaȱCDȱROMȱproducedȱforȱtheȱNationalȱLandȱandȱ WaterȱResourcesȱAuditȱ(NLWRA),ȱAGSOȱGeoScienceȱ Australia,ȱCanberraȱ Activity: Extension Age: High school Time: 2 Why? Different types of estuaries are very different in their mixing regimes and in how pollution is distributed. Understanding what type of estuary you are studying will help you in understanding why things happen the way they do in your estuary. Safety: This is a desk-based activity. – aȱgoodȱtopographicȱmapȱofȱyourȱestuaryȱ – aȱtideȱbookȱ – annualȱrainfallȱandȱevaporationȱtotalsȱ – accessȱtoȱtheȱinternetȱtoȱexploreȱtheȱOzEstuariesȱdatabaseȱonȱtheȱNLWRAȱwebsiteȱatȱ <www.ozestuaries.org>.ȱ What to do: FirstȱexploreȱtheȱCDȱROM.ȱCanȱyouȱunderstandȱtheȱmethodȱtheȱNLWRAȱuseȱinȱtheirȱclassificationȱofȱ estuaries?ȱLookingȱatȱyourȱestuary’sȱshapeȱonȱyourȱtopographicȱmap,ȱtheȱrangeȱofȱyourȱtideȱonȱyourȱtideȱ book,ȱandȱyourȱweatherȱdetails,ȱwhatȱtypeȱofȱestuaryȱdoȱyouȱthinkȱyouȱmayȱbeȱstudying?ȱ VisitȱtheȱOzEstuariesȱwebsite.ȱIsȱyourȱestuaryȱlistedȱonȱtheȱsite?ȱIfȱitȱis,ȱdidȱtheȱNLWRAȱcomeȱtoȱtheȱ sameȱclassificationȱasȱyouȱdid?ȱTheȱNLWRAȱonlyȱexaminedȱfairlyȱlargeȱestuaries,ȱsoȱyoursȱmayȱbeȱpartȱ ofȱanȱ‘amalgamated’ȱestuary.ȱIfȱyourȱestuaryȱisȱlisted,ȱpullȱupȱsomeȱofȱtheȱdetailedȱdataȱthatȱisȱenteredȱonȱ theȱwebsite.ȱIfȱyourȱestuaryȱwasȱtooȱsmallȱtoȱbeȱincluded,ȱlookȱatȱtheȱdataȱonȱaȱsimilarȱestuaryȱinȱtheȱ vicinityȱofȱyours.ȱDoesȱitȱhaveȱmuchȱinȱcommonȱwithȱyourȱestuary?ȱ Theȱdataȱonȱthisȱsiteȱmayȱprovideȱyouȱwithȱlotsȱofȱinformationȱaboutȱyourȱestuary,ȱandȱthisȱmayȱhelpȱ youȱinȱdecidingȱwhatȱaspectsȱofȱyourȱestuaryȱyouȱwishȱtoȱstudyȱfurther.ȱ ȱ ȱ 40 ESTUARINE MONITORING GUIDANCE MANUAL Mapping the salinity gradient of your estuary Manyȱwaterȱqualityȱmonitoringȱprogramsȱareȱbasedȱonȱtakingȱaȱ waterȱsampleȱregularlyȱatȱtheȱsameȱlocationȱeachȱtime.ȱThisȱ monitoringȱisȱaȱvalidȱmethodȱofȱensuringȱreplicableȱsampleȱresults,ȱ althoughȱtheȱsamplesȱmayȱnotȱtellȱyouȱmuchȱaboutȱtheȱrestȱofȱyourȱ estuary.ȱ Estuariesȱhaveȱaȱgradientȱofȱsalinityȱalongȱthem,ȱstartingȱwithȱeitherȱ freshwaterȱ(inȱpositiveȱestuaries)ȱorȱhypersalineȱwaterȱ(inȱnegativeȱ estuaries)ȱatȱtheȱheadȱofȱtheȱestuary,ȱandȱeitherȱincreasingȱorȱ decreasingȱinȱsalinityȱuntilȱatȱtheȱmouthȱofȱtheȱestuaryȱtheȱsalinityȱisȱ thatȱofȱseawaterȱ(eusaline).ȱȱ Toȱlookȱatȱtheȱsalinityȱgradientȱinȱyourȱestuary,ȱmultipleȱsamplesȱ needȱtoȱbeȱtakenȱalongȱtheȱestuary–alongȱtheȱedgesȱofȱtheȱestuaryȱȱ andȱasȱcloseȱtoȱmidstreamȱasȱyouȱcanȱgetȱ(usuallyȱfromȱaȱbridge,ȱorȱ fromȱaȱjettyȱorȱboat).ȱ Activity: 3 Extension Age: High school Time: 4 Why? Estuaries vary in salinity from their head to their mouth. Safety: Salinity gradient mapping may require working over deep water. What you may need: – salinityȱprofileȱandȱgradientȱrecordȱformȱ – aȱsamplingȱtubeȱonȱaȱpoleȱ – equipmentȱtoȱmeasureȱsalinityȱandȱtemperatureȱ – GPSȱtoȱdetermineȱyourȱlocationȱifȱusingȱaȱboat,ȱorȱgoodȱtopographicȱmapȱifȱmeasuringȱfromȱaȱ jettyȱorȱbridgeȱ – samplingȱplatformȱ(seaworthyȱboat,ȱbridge,ȱjetty).ȱ To ensure good results: Checkȱyourȱequipmentȱpriorȱtoȱgoingȱoutȱintoȱtheȱfield.ȱSeeȱtheȱmethodȱguidelinesȱMeasuringȱsalinityȱȱ andȱMeasuringȱtemperatureȱforȱdetails.ȱ What to do: Identifyȱyourȱsampleȱlocationȱandȱgetȱthere.ȱRecordȱsiteȱdetailsȱsuchȱasȱlocationȱcoordinatesȱifȱsamplingȱ fromȱaȱboat.ȱOftenȱaȱseriesȱofȱseveralȱlowȬlevelȱbridgesȱareȱidealȱforȱthisȱactivity,ȱasȱyouȱmayȱsampleȱonȱ bothȱedgesȱofȱtheȱestuaryȱandȱtheȱmiddle,ȱatȱseveralȱlocationsȱalongȱtheȱestuary.ȱ Sampleȱwaterȱfromȱaboutȱ200–mmȱbelowȱtheȱsurface.ȱOnceȱsamplesȱareȱcollected,ȱtestȱthemȱforȱsalinityȱ andȱtemperature.ȱSeeȱtheȱmethodȱguidelinesȱMeasuringȱsalinityȱandȱMeasuringȱtemperatureȱforȱdetails.ȱ Recordȱtheȱresultsȱforȱeachȱlocation.ȱ Onȱreturnȱtoȱbase,ȱplotȱtheȱresultsȱonȱaȱmapȱofȱtheȱestuary.ȱ Thisȱtypeȱofȱinformationȱisȱveryȱusefulȱwhenȱmakingȱmanagementȱorȱmonitoringȱdecisionsȱaboutȱanȱ estuaryȱ(orȱanyȱcoastalȱwaterȱbody).ȱMoreȱsuitableȱlocationsȱforȱdischargingȱstormwaterȱorȱcleanȱ saltwaterȱeffluentsȱ(forȱexampleȱfromȱindustrialȱevaporativeȱairȱconditioningȱplants,ȱwaterȱsofteningȱ plants,ȱandȱreverseȱosmosisȱplants)ȱmayȱbeȱselectedȱbasedȱonȱtheȱsalinityȱgradientȱofȱyourȱestuary.ȱ 41 ESTUARINE MONITORING GUIDANCE MANUAL Mapping the salinity profile of your estuary Manyȱwaterȱqualityȱmonitoringȱprogramsȱareȱbasedȱonȱtakingȱaȱ waterȱsampleȱregularlyȱatȱtheȱsameȱlocationȱandȱatȱtheȱsameȱdepthȱ eachȱtime.ȱThisȱmonitoringȱisȱaȱvalidȱmethodȱofȱensuringȱreplicableȱ sampleȱresults,ȱbutȱmayȱnotȱgiveȱaȱrepresentativeȱresultȱinȱstratifiedȱ estuaries.ȱ Stratification,ȱorȱlayering,ȱofȱdifferentȱsalinityȱwatersȱoftenȱoccursȱinȱ estuaries,ȱparticularlyȱinȱtheȱnegativeȱestuariesȱthatȱareȱsoȱcommonȱinȱ SouthȱAustralia.ȱHighlyȱsalineȱwaterȱisȱheavierȱthanȱseawater,ȱsoȱ whenȱtheȱtideȱcomesȱinȱorȱaȱstormwaterȱdischargeȱoccurs,ȱtheȱfresherȱ waterȱflowsȱoverȱtheȱtopȱofȱtheȱmoreȱsalineȱwater.ȱStratificationȱcanȱ resultȱinȱreducedȱdissolvedȱoxygenȱlevelsȱinȱtheȱdeeperȱlayers.ȱ Toȱgetȱaȱrepresentativeȱsampleȱinȱaȱstratifiedȱestuary,ȱmultipleȱ samplesȱneedȱtoȱbeȱtakenȱatȱaȱrangeȱofȱdepthsȱasȱcloseȱtoȱmidstreamȱ asȱyouȱcanȱget,ȱusuallyȱfromȱaȱbridge,ȱorȱfromȱaȱjettyȱorȱboat.ȱThisȱ methodȱisȱcalledȱprofiling.ȱ Activity: 3 Extension Age: High school Time: 3 Why? Stratified estuaries have quite different water quality at different depths. To determine how a pollutant will be distributed in an estuary it is necessary to understand the stratification pattern. Safety: Salinity profiling is conducted in deep water. Aȱcommonȱrangeȱofȱsamplingȱdepthsȱwouldȱinclude:ȱ200ȱmmȱbelowȱtheȱsurface,ȱoneȱmetre,ȱtwoȱmetresȱ andȱthenȱeveryȱtwoȱmetresȱafterȱthat,ȱuntilȱtheȱbottomȱofȱtheȱwaterȱbodyȱisȱreached.ȱIfȱyourȱestuaryȱisȱ particularlyȱshallow,ȱyouȱmayȱlikeȱtoȱmakeȱtheȱsamplingȱdepthsȱcloserȱtogether.ȱOptimallyȱyouȱwouldȱ takeȱatȱleastȱthreeȱsamplesȱ(bottom,ȱmiddleȱandȱnearȱsurface)ȱfromȱeachȱlocation.ȱSalinity,ȱdissolvedȱ oxygenȱ(DO),ȱpHȱandȱtemperatureȱreadingsȱareȱtakenȱforȱeachȱsample.ȱȱ Profilesȱareȱusuallyȱmadeȱatȱaȱnumberȱofȱlocationsȱtoȱproduceȱaȱsalinityȱstratificationȱmapȱofȱtheȱestuary,ȱ whichȱaidsȱinȱselectingȱtheȱmostȱrepresentativeȱsiteȱforȱregularȱsampling.ȱȱ What you may need: – salinityȱprofileȱandȱgradientȱrecordȱformȱ – depthȱsamplingȱdevice,ȱmarkedȱwithȱ500Ȭmmȱintervals–theseȱmayȱbeȱpurchasedȱorȱhomemadeȱ (seeȱAppendixȱ6ȱforȱusefulȱdesigns)ȱ – multipleȱsampleȱbottlesȱwithȱlidsȱ – equipmentȱtoȱmeasureȱsalinityȱandȱtemperatureȱ(DOȱandȱpHȱareȱoptional)ȱȱ – alternativelyȱyouȱcouldȱreplaceȱtheȱlastȱtwoȱitemsȱwithȱaȱmultiȬparameterȱprobeȱthatȱreadsȱEC,ȱ DO,ȱpHȱandȱtemperatureȱwithȱaȱsixȬmetreȱcableȱ – GPSȱtoȱdetermineȱyourȱlocationȱifȱusingȱaȱboat,ȱorȱgoodȱtopographicȱmapȱifȱmeasuringȱfromȱaȱ jettyȱorȱbridgeȱ – samplingȱplatformȱ(seaworthyȱboat,ȱbridge,ȱjetty).ȱ To ensure good results: Tryȱtoȱreachȱyourȱsamplingȱlocationȱwithoutȱdisturbingȱtheȱwaterȱunduly.ȱDisturbingȱtheȱwaterȱmayȱmixȱ theȱstratificationȱlayers.ȱ Checkȱyourȱequipmentȱpriorȱtoȱgoingȱoutȱintoȱtheȱfield.ȱSeeȱtheȱmethodȱpagesȱMeasuringȱtemperature,ȱ MeasuringȱpH,ȱȱMeasuringȱsalinityȱandȱMeasuringȱdissolvedȱoxygenȱforȱdetails.ȱ What to do: Identifyȱyourȱsampleȱlocationȱandȱgetȱthere.ȱRecordȱlocationȱdetailsȱsuchȱasȱcoordinates,ȱifȱsamplingȱ fromȱaȱboat.ȱ ȱ ȱ 42 ESTUARINE MONITORING GUIDANCE MANUAL Useȱyourȱsamplingȱdeviceȱtoȱcollectȱwaterȱsamplesȱatȱseveralȱdepths.ȱMostȱsamplersȱworkȱbyȱremovingȱ theȱlidȱofȱtheȱbottlesȱafterȱreachingȱpredeterminedȱdepths.ȱSamplersȱmayȱtestȱseveralȱdepthsȱatȱonceȱorȱ mayȱsampleȱsingleȱbottlesȱatȱaȱtime.ȱInȱtheȱlatterȱcase,ȱrepeatȱtheȱcollectionȱprocessȱuntilȱaȱsampleȱhasȱ beenȱcollectedȱatȱeachȱdesiredȱdepth.ȱWhenȱtakingȱrepeatedȱsamples,ȱcollectȱtheȱshallowestȱsamplesȱfirst,ȱ soȱasȱnotȱtoȱdisturbȱtheȱstratification.ȱ Onceȱsamplesȱareȱcollected,ȱremoveȱthemȱfromȱtheȱwaterȱandȱtestȱtheȱsamplesȱforȱtheȱdesiredȱwaterȱ qualityȱparametersȱ(salinity,ȱtemperature,ȱandȱmaybeȱpHȱandȱDO).ȱ Theȱresultsȱwillȱrevealȱifȱthereȱareȱanyȱstratifiedȱlayersȱinȱtheȱwaterȱcolumnȱatȱyourȱsamplingȱlocation.ȱIfȱ thereȱareȱnoȱobviousȱlayers,ȱitȱshowsȱthatȱyourȱestuaryȱhasȱgoodȱmixingȱatȱthatȱlocation,ȱonȱthatȱday.ȱ Differencesȱinȱtemperatureȱofȱonlyȱ2°Cȱorȱsalinityȱchangesȱofȱaȱcoupleȱofȱgramsȱperȱlitreȱmayȱbeȱ sufficientȱtoȱproduceȱaȱstratificationȱlayer.ȱȱ Lowȱoxygenȱlevelsȱinȱtheȱdeeperȱsamplesȱareȱaȱgoodȱindicatorȱofȱslowȱverticalȱmixing.ȱThisȱmeansȱthatȱ theȱanimalsȱandȱplantsȱonȱtheȱfloorȱofȱtheȱestuaryȱwillȱbeȱthoseȱthatȱrequireȱlittleȱoxygenȱorȱthoseȱthatȱ reduceȱsulphurȱcompoundsȱtoȱextractȱoxygen.ȱȱ Thisȱtypeȱofȱinformationȱisȱveryȱusefulȱwhenȱmakingȱmanagementȱorȱmonitoringȱdecisionsȱaboutȱanȱ estuaryȱ(orȱanyȱtypeȱofȱcoastalȱwaterȱbody).ȱStratificationȱisȱmoreȱlikelyȱtoȱoccurȱinȱareasȱthatȱhaveȱsmallȱ tidalȱranges,ȱwhichȱexplainsȱwhyȱstratificationȱisȱsoȱcommonȱinȱSouthȱAustralianȱestuaries.ȱInflowsȱofȱ freshwaterȱmayȱalsoȱcauseȱtemporaryȱstratificationȱlayers.ȱ ȱ ȱ ȱ 43 ESTUARINE MONITORING GUIDANCE MANUAL Mapping turbidity plumes MappingȱturbidityȱplumesȱisȱanȱeventȬbasedȱmonitoringȱactivity.ȱ EventȬbasedȱmonitoringȱisȱanȱimportant,ȱbutȱlabourȱintensiveȱandȱ timeȱsensitive.ȱForȱsuccessfulȱturbidityȱplumeȱmapping,ȱpeopleȱneedȱ toȱrespondȱrapidlyȱandȱbeȱavailableȱatȱaȱmoment’sȱnotice.ȱThisȱtypeȱ ofȱmonitoringȱisȱdesignedȱtoȱcaptureȱtheȱeffectsȱofȱrunoffȱeventsȱ (particularlyȱtheȱfirstȱlargeȱeventȱinȱaȱwetȱseason)ȱonȱtheȱestuarineȱ receivingȱwaters.ȱȱ Mappingȱofȱaȱturbidityȱplumeȱisȱdoneȱimmediatelyȱafterȱorȱduringȱ theȱfirstȱmajorȱrunoffȱeventȱforȱtheȱseason.ȱAȱboatȱisȱusedȱtoȱgoȱoutȱ andȱmapȱtheȱextentȱofȱaȱturbidityȱplumeȱfromȱaȱstormwaterȱ dischargeȱpointȱorȱtributary.ȱTheȱmajorȱdischargeȱpointȱforȱyourȱ estuaryȱneedsȱtoȱbeȱidentifiedȱpriorȱtoȱtheȱevent.ȱȱ WaterȱclarityȱmeasurementsȱtakenȱwithȱaȱSecchiȱdisk,ȱorȱturbidityȱ measuredȱwithȱaȱmeterȱareȱtheȱonlyȱparametersȱrequiredȱinȱthisȱ activity.ȱHowever,ȱpH,ȱsalinity,ȱdissolvedȱoxygenȱandȱnutrients,ȱ particularlyȱofȱtheȱdischarge,ȱcouldȱalsoȱbeȱmeasuredȱifȱyouȱhaveȱtheȱ time.ȱȱ Turbidityȱmapsȱareȱveryȱimportantȱwhenȱtryingȱtoȱmanageȱanȱ estuaryȱandȱitsȱassociatedȱcatchment.ȱTheseȱmapsȱmeasureȱtheȱ immediateȱdecreaseȱinȱwaterȱclarityȱfromȱaȱrunoffȱevent.ȱInȱ catchmentsȱwithȱgoodȱlandȱmanagementȱpracticesȱthisȱimpactȱisȱ generallyȱfairlyȱsmall.ȱTheȱbiggerȱtheȱimpact,ȱtheȱworseȱtheȱlandȱ managementȱpracticesȱinȱtheȱcatchmentȱgenerallyȱare.ȱȱ Theȱfineȱsedimentsȱthatȱcauseȱtheȱdecreasedȱwaterȱclarityȱgenerallyȱ bringȱotherȱpollutantsȱwithȱthem.ȱTheseȱcanȱincludeȱnutrients,ȱheavyȱ metalsȱandȱhydrocarbons.ȱTheȱfirstȱflushȱofȱfloodwatersȱisȱgenerallyȱ theȱworst,ȱbutȱisȱoftenȱtheȱhardestȱtoȱcaptureȱwithȱmonitoring.ȱOtherȱ effectsȱofȱstormwaterȱcanȱbeȱalkalineȱorȱacidȱrunoff,ȱchemicalȱ pollutantsȱthatȱdepleteȱoxygenȱconcentrationsȱandȱfaecalȱbacteria.ȱByȱ mappingȱtheȱturbidity,ȱitȱisȱpossibleȱtoȱestimateȱhowȱotherȱpollutantsȱ fromȱtheȱeventȱwillȱdisperseȱintoȱtheȱestuary.ȱ Anotherȱturbidityȱmapȱcanȱbeȱcreatedȱaȱcoupleȱofȱdaysȱafterȱtheȱeventȱ toȱgiveȱanȱideaȱofȱhowȱfastȱtheȱplume,ȱwithȱitsȱassociatedȱpollutants,ȱ dispersesȱintoȱtheȱecosystem.ȱȱ What you may need: Activity: Age: High school Time: 4 Why? Turbidity plumes reveal where the impacts of stormwater pollution are likely to be found. Safety: This activity is completed either during rain events or when storm water is flowing. Floodwaters can be dangerous. Never stand in water above knee-height if it is flowing at any speed. Always use some form of sampling device if trying to take a sample in fast-flowing water, as you can let go of it if the flow tries to pull you in. Sample from bridges or other structures rather than a boat if the flows are too great. Do not go out in lightning storms, when visibility is bad or when there is a heavy weather warning out for boats in coastal areas. Check the BOM website to make sure. IF IN DOUBT— DON’T GO OUT. Find some other way to gather the data, or wait until some other runoff event. – eventȱmonitoringȱformȱ – Secchiȱdiskȱ – sampleȱbottlesȱandȱtestȱequipmentȱforȱanyȱotherȱparametersȱbeingȱtestedȱȱ – seaworthyȱboatȱandȱallȱnecessaryȱsafetyȱequipmentȱ – GPSȱforȱlocatingȱsitesȱ – atȱleastȱtwoȱpeopleȱinȱtheȱboatȱandȱaȱcoupleȱonȱtheȱshoreȱ – aȱgoodȱtopographicȱmapȱorȱaȱbathymetricȱmapȱofȱtheȱestuary.ȱ 44 3 Extension ESTUARINE MONITORING GUIDANCE MANUAL To ensure good results: Identifyȱtheȱmainȱstormwaterȱdrainsȱenteringȱyourȱestuary.ȱYourȱlocalȱcouncilȱmayȱbeȱableȱtoȱhelpȱyou.ȱ Checkȱyourȱtestȱequipment,ȱpriorȱtoȱgoingȱoutȱonȱsite.ȱSeeȱtheȱmethodȱpageȱMeasuringȱwaterȱclarity.ȱȱ ȱ What to do: Collectȱallȱofȱyourȱgearȱtogetherȱandȱensureȱallȱyourȱtestȱandȱsafetyȱequipmentȱisȱstoredȱinȱtheȱboat.ȱWaitȱ forȱaȱrainȱevent.ȱIfȱwaterȱisȱrunningȱdownȱtheȱnearbyȱstormwaterȱdrains,ȱitȱwillȱonlyȱbeȱaȱmatterȱofȱ minutesȱorȱaȱcoupleȱofȱhoursȱatȱmostȱbeforeȱitȱstartsȱtoȱenterȱtheȱestuary.ȱOnceȱwaterȱisȱseenȱrunningȱintoȱ theȱnearbyȱdrainsȱinȱanyȱquantity,ȱgatherȱtheȱmonitoringȱgroupȱtogetherȱandȱgoȱcheckȱyourȱmajorȱ stormwaterȱdischargeȱlocation.ȱȱ Onceȱallȱsafetyȱissuesȱareȱassessedȱandȱaddressed,ȱandȱstormwaterȱisȱcomingȱoutȱofȱtheȱstormwaterȱ drain,ȱlaunchȱyourȱboatȱandȱgoȱtoȱtheȱdischargeȱpoint.ȱTakeȱSecchiȱdiskȱreadingsȱandȱcollectȱaȱsampleȱ(ifȱ required)ȱatȱtheȱdischargeȱpoint.ȱȱ UsingȱyourȱGPSȱasȱaȱguide,ȱmoveȱdirectlyȱoutȱfromȱtheȱdischargeȱpointȱandȱtakeȱanotherȱSecchiȱdepthȱorȱ turbidityȱreading.ȱContinueȱawayȱfromȱtheȱdischargeȱpoint,ȱtakingȱSecchiȱdepthsȱandȱrecordingȱthemȱ withȱyourȱGPSȱlocationȱuntilȱtheȱSecchiȱdepthȱstopsȱincreasing.ȱThisȱisȱtheȱextentȱofȱtheȱplume.ȱȱ Useȱaȱsimilarȱprocedureȱtoȱworkȱoutȱtheȱwidthȱofȱtheȱplume,ȱtakingȱwaterȱsamplesȱifȱyouȱwishȱtoȱdoȱ chemicalȱanalysesȱofȱthemȱonȱtheȱboatȱorȱlater.ȱȱ TheȱdepthȱofȱaȱplumeȱinȱveryȱstratifiedȱestuariesȱmayȱbeȱestimatedȱbyȱusingȱaȱmultiȬparameterȱprobeȱonȱ aȱlongȱcable,ȱthatȱrecordsȱpH,ȱEC,ȱDOȱandȱturbidity.ȱToȱgaugeȱtheȱdepthȱofȱaȱplumeȱmoreȱaccurately,ȱ sampleȱtheȱwaterȱforȱsuspendedȱsolidsȱusingȱaȱdepthȬsamplingȱdevice.ȱSeeȱMappingȱtheȱsalinityȱprofileȱofȱ yourȱestuaryȱforȱmoreȱdetailsȱonȱsamplingȱstratifiedȱestuaries.ȱ DataȱfromȱeventȬbasedȱwaterȱclarityȱorȱturbidityȱmappingȱcanȱbeȱcomparedȱwithȱdataȱcollectedȱduringȱ normalȱmonthlyȱsampling.ȱThisȱinformationȱwillȱidentifyȱtheȱextentȱthatȱtheȱvaluesȱofȱdifferentȱ parametersȱmayȱbeȱexpectedȱtoȱchangeȱbetweenȱnormalȱandȱstormȱevents.ȱȱ ȱ ȱ ȱ 45 ESTUARINE MONITORING GUIDANCE MANUAL Where is the ‘turbidity maximum’? Inȱestuariesȱtheȱwaterȱinȱtheȱupriverȱpartȱofȱtheȱestuaryȱandȱtheȱwaterȱ inȱtheȱmarineȱpartȱofȱtheȱestuaryȱareȱusuallyȱfairlyȱclearȱ(exceptȱ whereȱveryȱturbidȱwaterȱisȱcomingȱfromȱaȱriver,ȱsuchȱasȱtheȱDarlingȱ RiverȱflowsȱinȱtheȱMurray–Darlingȱsystem).ȱWithinȱtheȱmainȱpartȱofȱ theȱestuary,ȱhowever,ȱtheȱwaterȱisȱoftenȱveryȱturbid,ȱhighlyȱchargedȱ withȱsuspendedȱsediment.ȱȱ Thisȱisȱknownȱasȱtheȱturbidityȱmaximum.ȱTheȱsedimentȱmovesȱupȱ andȱdownȱtheȱestuaryȱwithȱtheȱdailyȱtide.ȱItȱmayȱbecomeȱdilutedȱ duringȱhighȱfloodȱeventsȱorȱdisappearȱduringȱstrongȱtidalȱflow,ȱbutȱ mayȱreformȱduringȱneapȱtidalȱcycles.ȱ Theȱphenomenonȱoccursȱbecauseȱveryȱfineȱparticlesȱthatȱareȱcarriedȱ inȱriverȱwaterȱcomeȱintoȱcontactȱwithȱseawaterȱthatȱcontainsȱsaltsȱ suchȱasȱcarbonates.ȱTheseȱhelpȱtheȱfineȱparticlesȱtoȱaggregate,ȱ formingȱlarge,ȱsoftȱflocs.ȱTheȱflocsȱareȱheavierȱthanȱtheȱfineȱparticlesȱ were,ȱandȱtheyȱstartȱtoȱsettleȱtoȱtheȱbottomȱofȱtheȱestuary.ȱHowever,ȱ theyȱdoȱnotȱformȱsolidȱsedimentsȱonȱtheȱbaseȱofȱtheȱestuary,ȱandȱsoȱ mayȱbeȱresuspendedȱwithȱtheȱnextȱincomingȱtide.ȱThusȱtheȱzoneȱ receivesȱaȱcontinuousȱnewȱsupplyȱofȱnewȱsediment,ȱwhileȱ resuspendingȱsomeȱofȱtheȱsedimentȱalreadyȱpresent.ȱ Activity: Extension Age: High school Time: 4 Why? The turbidity maximum in an estuary is the zone where much of the sediment from the river is settled out. The zone forms an important part of many estuarine food chains, but may also be the location where accumulations of toxic metals may occur. Safety: This may involve work in deep water. Theȱturbidityȱmaximumȱforȱaȱgivenȱestuaryȱisȱtheȱareaȱwhereȱmostȱsoftȱsedimentsȱareȱdeposited,ȱandȱ thisȱisȱtheȱareaȱwhereȱmetals,ȱetc,ȱinȱtheȱriverȱwaterȱwillȱprecipitate.ȱThusȱtheȱturbidityȱmaximaȱofȱ estuariesȱperformȱanȱessentialȱcleaningȱfunctionȱofȱtheȱriverȱwaterȱbeforeȱitȱentersȱtheȱsea.ȱTheȱzoneȱisȱ alsoȱaȱrichȱfeedingȱzoneȱforȱsmallȱmacroȱinvertebrates,ȱandȱthereforeȱprovidesȱtheȱbasisȱofȱmanyȱ estuarineȱfoodȱchains.ȱ What you may need: – eventȱmonitoringȱformȱ – Secchiȱdiskȱ – seaworthyȱboatȱandȱallȱnecessaryȱsafetyȱequipmentȱ – GPSȱforȱlocatingȱsitesȱ – atȱleastȱtwoȱpeopleȱinȱtheȱboatȱandȱaȱcoupleȱonȱtheȱshoreȱ – aȱgoodȱtopographicȱmapȱorȱaȱbathymetricȱmapȱofȱtheȱestuary,ȱandȱpossiblyȱanȱaerialȱphotograph.ȱ To ensure good results: Onlyȱconductȱthisȱactivityȱinȱcalmȱconditionsȱwithȱlowȱtidalȱflowsȱ(neapȱtides).ȱAtȱthisȱtime,ȱtheȱturbidityȱ maximumȱwillȱbeȱmoreȱpronouncedȱandȱeasierȱtoȱdetect.ȱ Checkȱyourȱtestȱequipment,ȱpriorȱtoȱgoingȱoutȱonȱsite.ȱ What to do: Reviewȱyourȱtopographicȱmaps,ȱbathymetricȱmapsȱandȱaerialȱphotographs.ȱYouȱmayȱbeȱableȱtoȱpickȱtheȱ roughȱlocationȱofȱtheȱturbidityȱmaximumȱfromȱturbidityȱvisibleȱinȱtheȱaerialȱphotograph.ȱUseȱyourȱotherȱ mapsȱtoȱlookȱforȱareasȱofȱdepositionȱ(sandbanksȱonȱtheȱbathymetricȱmaps,ȱinȬstreamȱsandyȱislandsȱonȱ theȱtopographicȱmaps),ȱandȱtheȱchannelsȱaroundȱtheseȱareas.ȱ Collectȱallȱofȱyourȱgearȱandȱensureȱallȱyourȱtestȱandȱsafetyȱequipmentȱisȱstoredȱinȱtheȱboat.ȱWaitȱforȱcalmȱ weatherȱandȱaȱneapȱtide.ȱOnceȱyouȱareȱsureȱtheȱconditionsȱareȱstable,ȱgatherȱtheȱmonitoringȱgroupȱandȱ goȱoutȱintoȱtheȱestuary.ȱȱ 46 ESTUARINE MONITORING GUIDANCE MANUAL Startȱfromȱtheȱmarineȱendȱofȱtheȱestuary,ȱorȱatȱleastȱwellȱonȱtheȱmarineȱsideȱofȱtheȱareaȱthatȱyouȱhaveȱ decidedȱmayȱcontainȱyourȱturbidityȱmaximum.ȱTakeȱSecchiȱdiskȱreadingsȱatȱregularȱintervalsȱasȱyouȱ progressȱupstream,ȱrecordingȱeachȱlocationȱusingȱyourȱGPS.ȱTheȱguidelineȱMeasuringȱwaterȱclarityȱ containsȱdetailsȱaboutȱusingȱtheȱSecchiȱdisk.ȱ Theȱturbidityȱmaximumȱwillȱbeȱrevealedȱasȱaȱseriesȱofȱlowȱclarityȱreadings.ȱ Identifyingȱtheȱlocationȱofȱyourȱestuary’sȱturbidityȱmaximumȱassistsȱenvironmentalȱmanagersȱwhoȱmayȱ beȱstudyingȱpollutantȱaccumulationȱinȱtheȱestuary.ȱTheȱcomplexȱflocsȱthatȱareȱformedȱinȱthisȱzoneȱareȱaȱ mixtureȱofȱorganicȱandȱinorganicȱmaterials,ȱandȱsoȱthisȱzoneȱmayȱalsoȱcontainȱaȱlargeȱpopulationȱofȱ macroȱinvertebrates.ȱStudentsȱworkingȱonȱestuarineȱmacroȱinvertebratesȱmayȱfindȱthatȱknowingȱtheȱ locationȱofȱestuarineȱturbidityȱmaximaȱprovidesȱthemȱwithȱfurtherȱunderstandingȱaboutȱtheȱ distributionsȱofȱcertainȱspeciesȱwithinȱestuaries.ȱ ȱ ȱ ȱ 47 ESTUARINE MONITORING GUIDANCE MANUAL Effect of tidal restrictions on wetlands Studyingȱtheȱeffectȱofȱtidalȱrestrictionsȱonȱtheȱwetlandsȱthatȱsurroundȱ yourȱestuaryȱisȱaȱfascinatingȱwayȱofȱdiscoveringȱestuarineȱprocesses.ȱ Estuarineȱwetlandsȱdevelopȱtheirȱcharacterȱfromȱtheȱhydrologicalȱ regimeȱofȱtheȱestuary.ȱMonitoringȱtheȱgroundȱandȱsurfaceȱwaterȱ qualityȱandȱquantityȱcanȱprovideȱanȱinsightȱintoȱtheȱhealthȱandȱlongȬȱ termȱchangesȱinȱtheseȱwetlands.ȱȱ Tidalȱrestrictionsȱmayȱcauseȱaȱwideȱrangeȱofȱbiological,ȱecological,ȱ chemicalȱandȱphysicalȱeffects.ȱAreasȱwithȱnaturalȱorȱanthropogenicȱ restrictionsȱonȱtidalȱmovementȱoftenȱhaveȱhigherȱsalinitiesȱorȱsalinityȱ rangesȱthanȱareasȱthatȱhaveȱfreeȱtidalȱflushing.ȱIfȱthereȱisȱaȱlargeȱ regularȱfreshwaterȱinputȱtoȱaȱtidallyȱrestrictedȱareaȱtheȱlackȱofȱtidalȱ flushingȱmayȱresultȱinȱreducedȱsalinity.ȱWhenȱsalinityȱlevelsȱfallȱ belowȱ20ȱg/Lȱorȱ2%,ȱopportunisticȱbrackishȱplantsȱsuchȱasȱPhragmitesȱ australisȱ(commonȱreed)ȱmayȱstartȱtoȱtakeȱoverȱtheȱsamphireȱareas.ȱ ManyȱhistoricȱdevelopmentsȱinȱSouthȱAustraliaȱdidȱnotȱconsiderȱtheȱ impactsȱofȱtidalȱrestriction.ȱ Tideȱrestrictionsȱmayȱblockȱtheȱpassageȱofȱestuarineȱinvertebratesȱ andȱfishȱintoȱtheȱupperȱestuary.ȱRestrictedȱareasȱmayȱalsoȱsufferȱfromȱ aȱconcentrationȱofȱsediments,ȱorganicȱmatterȱandȱwaterȱpollutantsȱinȱ theȱmarshes,ȱsoȱtheyȱbecomeȱanoxicȱandȱsmelly.ȱ SedimentsȱthatȱwereȱonceȱregularlyȱsubmergedȱinȱsulphateȬrichȱ seawaterȱmayȱsinkȱrapidlyȱwhenȱtheȱseawaterȱisȱdrainedȱoffȱandȱtheȱ sedimentsȱareȱexposedȱtoȱair.ȱThisȱisȱbecauseȱmanyȱmarineȱsedimentsȱ containȱlargeȱquantitiesȱofȱshellȱgritȱ(calciumȱcarbonate).ȱSulphateȱ compoundsȱleftȱbehindȱbyȱtheȱseawaterȱareȱconvertedȱtoȱhydrogenȱ sulphideȱbyȱsulphurȬreducingȱbacteria.ȱHydrogenȱsulphideȱmayȱ convertȱtoȱsulphuricȱacidȱinȱcontactȱwithȱtheȱair,ȱandȱthisȱrapidlyȱ reactsȱwithȱtheȱcalciumȱcarbonateȱinȱtheȱshellȱgrit.ȱThisȱresultsȱinȱ carbonȱdioxideȱoffȬgassingȱfromȱtheȱsoilȱandȱcalciumȱsulphateȱ enteringȱtheȱgroundwater,ȱeffectivelyȱremovingȱsolidȱmatterȱfromȱtheȱ soil.ȱ Knowledgeȱofȱtideȱrestrictions,ȱtheirȱtypeȱandȱpotentialȱeffectsȱisȱ essentialȱforȱcomprehensiveȱsaltmarshȱmonitoringȱandȱassessment.ȱ Tidalȱinfluenceȱisȱanȱimportantȱparameterȱtoȱmeasure,ȱalongȱwithȱ tidalȱrangeȱandȱsalinity.ȱTheseȱthreeȱattributesȱcanȱprovideȱaȱveryȱ goodȱunderstandingȱofȱtheȱeffectȱofȱtideȱrestrictionsȱonȱtheȱphysicalȱ andȱchemicalȱnatureȱofȱsaltmarshes.ȱ Thisȱactivityȱoutlinesȱtheȱbasicȱmethodsȱusedȱtoȱmonitorȱtidalȱ restrictions.ȱItȱisȱenvisagedȱthatȱcommunityȱgroupsȱundertakingȱ restorationȱorȱrecoveryȱprogramsȱinȱaffectedȱestuariesȱwillȱfindȱtheȱ methodsȱusefulȱisȱassessingȱtheȱprogressȱofȱtheirȱprojects.ȱȱ Activity: 3 Extension Age: High school Time: 4 (initially, then 2 to monitor) Why? Estuarine wetlands develop their character (saltmarsh, melaleuca wetland, mangrove, or freshwater-tidal wetland) from the hydrological regime. Monitoring the ground and surface water quantity and quality can provide insight into the health of, and reasons for long-term change in, these wetlands. Safety: Saltmarshes contain rough terrain and ill– defined waterways. Participants should wear sturdy shoes, long pants and a hat. Participants should take care when walking so that they do not fall down undercut embankments or slide over in muddy sections. Working in pairs will help avoid and deal with any injuries that may occur in saltmarshes. Areas where tidal movement is restricted may have strong currents passing through channels or culverts. Care should be taken to avoid these, and avoid wading in fast flowing water. Theȱmethodsȱusedȱinȱthisȱactivityȱmeasureȱtheȱareaȱofȱtidalȱinfluence,ȱaverageȱtidalȱranges,ȱsalinityȱ concentrationsȱandȱpHȱchangesȱinȱareasȱthatȱhaveȱbeenȱaffectedȱbyȱtidalȱrestrictions.ȱThisȱisȱdoneȱbyȱ installingȱdepthȱgaugesȱinȱsamphireȱpondsȱandȱinȱtheȱunrestrictedȱwaterway,ȱasȱwellȱasȱpiezometersȱinȱ theȱsamphireȱuplandsȱ(highȱmarsh).ȱ Staffȱgaugesȱareȱessentiallyȱlargeȱrulersȱstakedȱinȱaȱfixedȱposition,ȱandȱareȱusedȱtoȱmeasureȱwaterȱlevels.ȱ Youȱcanȱconstructȱstaffȱgaugesȱfromȱaȱvarietyȱofȱmaterials,ȱandȱtheȱonlyȱrequirementsȱareȱthatȱtheyȱbeȱ durable,ȱrustproofȱandȱappropriateȱforȱtheȱsiteȱlocation,ȱbudget,ȱandȱneedsȱofȱtheȱinvestigators.ȱ 48 ESTUARINE MONITORING GUIDANCE MANUAL GroundwaterȱisȱsampledȱfromȱverticallyȱinstalledȱoneȬmetreȱlongȱPVCȱpipesȱknownȱasȱpiezometers.ȱ Theseȱareȱusedȱforȱmonitoringȱtheȱdepth,ȱsalinityȱandȱpHȱofȱtheȱgroundwaterȱtable.ȱGroundwaterȱinȱ saltmarshesȱisȱusuallyȱmonitoredȱonlyȱduringȱdryȱperiods,ȱalthoughȱsamplingȱduringȱwetȱperiodsȱmayȱ beȱinterestingȱfromȱaȱsalinityȱandȱpHȱperspective.ȱDuringȱwetȱperiodsȱtheȱgroundwaterȱwillȱusuallyȱbeȱ at,ȱorȱnear,ȱtheȱsurface.ȱȱ Theȱaverageȱtidalȱrangeȱisȱestimatedȱbyȱtakingȱregularȱobservationsȱofȱaȱsurveyedȱdepthȱorȱstaffȱgaugeȱ overȱaȱtidalȱperiodȱ(approximatelyȱsixȱhours)ȱatȱleastȱonceȱaȱyearȱduringȱaȱspringȱtide.ȱMonitoringȱ severalȱtimesȱaȱyearȱisȱpreferable.ȱȱ Theȱareaȱofȱtidalȱinfluenceȱisȱmeasuredȱbyȱobservingȱseveralȱdepthȱgaugesȱoverȱaȱtidalȱperiodȱtoȱ determineȱhowȱfarȱtheȱtideȱmovesȱintoȱtheȱsaltmarsh.ȱTheȱtidalȱinfluenceȱonȱgroundȱwaterȱcanȱalsoȱbeȱ measuredȱbyȱtakingȱseveralȱdepthȱobservationsȱdownȱpiezometersȱoverȱaȱtidalȱperiodȱorȱoverȱaȱday.ȱ Excellentȱreferenceȱbooksȱonȱtidalȱmarshȱrestorationȱare:ȱ Coultas,ȱCLȱ&ȱHsieh,ȱYȱ(eds)ȱ(1997),ȱEcologyȱandȱManagementȱofȱTidalȱMarshes—AȱModelȱfromȱtheȱGulfȱ ofȱMexico,ȱStȱLucieȱPress,ȱDelrayȱBeach,ȱFlorida.ȱ Packham,ȱJRȱ&ȱWillis,ȱAJȱ(1997),ȱEcologyȱofȱDunes,ȱSaltmarshȱandȱShingle.ȱChapmanȱandȱHall,ȱLondon.ȱ Purinton,ȱTAȱ&ȱMountain,ȱDCȱ(1997),ȱTidalȱCrossingȱHandbook—AȱVolunteerȱGuideȱtoȱAssessingȱTidalȱ Restrictions,ȱParkerȱRiverȱCleanȱWaterȱAssociation,ȱMassachesetts.ȱThisȱbookȱmayȱbeȱdownloadedȱ fromȱtheȱInternetȱfromȱ<www.parkerȬriver.org>.ȱ Zedler,ȱJB(ed)ȱ(2001),ȱHandbookȱforȱRestoringȱTidalȱWetlands,ȱCRCȱPress,ȱBocaȱRaton,ȱFlorida.ȱ What you may need: F or ȱ installing ȱst a ffȱg aug es ȱ – betweenȱtwoȱandȱsevenȱstaffȱgaugesȱ – aȱsurveyorȱorȱsurveyingȱequipmentȱ – largeȱsledgehammerȱorȱmallet.ȱ F or ȱm on it oring ȱs u r f ac eȱt id a lȱm ov eme nt ȱ – installedȱstaffȱgaugesȱ – hydrologyȱfieldȱdatasheetsȱandȱpencilsȱȱ – clockȱorȱwatchȱwithȱstopwatchȱȱ – clipboard.ȱȱ F or ȱc on struc t ing ȱ and ȱ installingȱp iez o met er s ȱ – tapeȱmeasureȱ – 50–75ȱmmȱPVCȱpipeȱ(oneȱmetreȱperȱpiezometer)ȱ – smallȱgardenȱstakeȱwithȱflagȱorȱbrightȱpaintȱ – drillȱwithȱaȱ0.5ȱtoȱ10–mmȱdrillȱbitȱ – twoȱendȬcapsȱperȱpiezometerȱtoȱfitȱtheȱPVCȱpipeȱ – someȱkneeȬhighȱstockingsȱ(twoȱperȱpiezometer)ȱ – GPSȱand/orȱaȱdetailedȱtopographicȱmapȱ – augurȱorȱpost–holeȱdiggerȱforȱboringȱaȱholeȱgreaterȱthanȱ100–mmȱȱ – bucketȱofȱcoarseȱsandȱorȱgravelȱ&ȱbucketȱofȱbentoniteȱȱ – smallȱamountȱofȱquicksetȱconcrete.ȱ 49 ESTUARINE MONITORING GUIDANCE MANUAL F or ȱgroundwaterȱ mon itoring ȱw o r kȱ – plopperȱorȱbeeperȱ(instructionsȱforȱmakingȱtheseȱareȱinȱAppendixȱ6),ȱorȱaȱthinȱstraightȱdryȱȱ woodenȱstakeȱlongerȱthanȱ1.3ȱmȱforȱeachȱpiezometerȱȱ – sampleȱflaskȱ&ȱtestȱequipmentȱandȱguidelinesȱforȱmeasuringȱforȱpHȱandȱsalinityȱȱ – balerȱorȱpetrolȱsiphonȱwithȱfoot–valveȱforȱremovingȱwaterȱfromȱtheȱwellȱȱ – distilledȱwaterȱtoȱcleanȱbaler(s)ȱȱ – measuringȱtape.ȱ What to do: Installing ȱ st a ffȱg aug esȱȱ Staffȱgaugesȱareȱgraduated,ȱmeaningȱthatȱunitsȱ(metres)ȱareȱclearlyȱwrittenȱonȱtheȱstaffȱsoȱthatȱtheȱwaterȱ levelȱcanȱbeȱrecordedȱbyȱsimplyȱlookingȱatȱtheȱgaugeȱ(Figureȱ5).ȱTheseȱgaugesȱshouldȱbeȱconstructedȱofȱ rustproofȱmaterialsȱthatȱcanȱwithstandȱwindȱandȱwaterȱcurrents.ȱȱ ItȱisȱcommonȱtoȱuseȱPVCȱpipeȱtoȱconstructȱgauges,ȱwithȱunitsȱmarkedȱ byȱetchingȱtheȱpipeȱthenȱfillingȱtheȱetchingsȱwithȱpaint.ȱAnotherȱ methodȱisȱtoȱuseȱaȱsheetȱofȱplasticȱorȱmelamineȱwithȱblackȱandȱwhiteȱ layers,ȱsoȱthatȱtheȱmarksȱmayȱbeȱetchedȱintoȱtheȱwhiteȱsideȱsoȱthatȱtheȱ blackȱlayerȱshowsȱthrough.ȱTheseȱareȱbestȱforȱinstallingȱdirectlyȱontoȱaȱ culvertȱorȱfenceȱpost,ȱandȱtheyȱwithstandȱdamageȱfromȱvandals.ȱ ȱ Figure 5—Staff gauge ȱ Findingȱaȱsuitableȱlocationȱforȱtheȱstaffȱgaugesȱisȱsometimesȱaȱ challenge.ȱVisitingȱtheȱstudyȱsiteȱatȱdifferentȱtidalȱstagesȱwillȱassistȱ you.ȱPlaceȱtheȱgaugesȱwhereȱthereȱisȱusuallyȱonlyȱaȱsmallȱamountȱofȱ water,ȱbutȱwhereȱyouȱcanȱeasilyȱreadȱthemȱwhenȱtheȱtideȱisȱhigh.ȱAȱ suggestedȱsetȱofȱlocationsȱforȱstaffȱgaugesȱisȱillustratedȱinȱtheȱexampleȱ siteȱplanȱbelowȱ(Figureȱ6).ȱȱ Toȱgaugeȱaȱrestriction,ȱyouȱshouldȱinstallȱstaffȱgaugesȱnoȱfurtherȱthanȱ 15ȱmȱfromȱtheȱtidalȱrestrictionȱonȱbothȱsidesȱofȱtheȱrestrictionȱ(egȱonȱ theȱrestrictedȱsideȱandȱtheȱunrestrictedȱside).ȱOther,ȱadditional,ȱ gaugesȱmayȱbeȱplacedȱinȱaȱtidalȱmarshȱtoȱmapȱtheȱareaȱofȱinundation.ȱ ȱ Figure 6—Staff gauge locations and piezometer transects 50 ESTUARINE MONITORING GUIDANCE MANUAL Gaugesȱonȱfreestandingȱpostsȱshouldȱnotȱbeȱplacedȱwhereȱstrongȱcurrentsȱoccurȱasȱtheyȱmayȱwashȱaway.ȱ Also,ȱitȱisȱdifficultȱtoȱreadȱtheȱwaterȱlevelȱifȱtheȱcurrentȱisȱcreatingȱlargeȱeddiesȱaroundȱtheȱpost.ȱInȱareasȱ subjectȱtoȱfrequentȱvandalism,ȱattachȱgaugesȱtoȱpreȬexistingȱimmovableȱobjectsȱwhereȱpossible,ȱforȱ exampleȱculvertsȱorȱrockȱembankments.ȱȱ Ifȱyouȱareȱinstallingȱfreestandingȱgauges,ȱtwoȱpeopleȱmayȱbeȱnecessaryȱtoȱinstallȱtheȱgauges–oneȱtoȱholdȱ theȱgaugeȱpostȱwhileȱtheȱotherȱpoundsȱitȱintoȱtheȱsubstrateȱwithȱaȱsledgehammerȱorȱmallet.ȱToȱbeȱsureȱ youȱaccuratelyȱplaceȱtheȱgauge,ȱinstallȱtheȱpost,ȱthenȱattachȱtheȱstaffȱmarkingȱplate.ȱBeȱsureȱtoȱpositionȱ theȱstaffȱgaugeȱsoȱthatȱtheȱheightȱmarksȱareȱreadilyȱvisibleȱbyȱtheȱobserver.ȱ Yourȱlocalȱcouncilȱmayȱbeȱpreparedȱtoȱassistȱyouȱsurveyȱstaffȱgaugeȱlocationsȱandȱinstallȱthem,ȱasȱaȱ surveyorȱ(orȱatȱleastȱtheirȱequipment)ȱisȱrequiredȱtoȱplaceȱtheȱmarkersȱatȱaȱknownȱheightȱrelativeȱtoȱ AHDȱ(AustralianȱHeightȱDatum).ȱGaugesȱshouldȱbeȱinstalledȱatȱleastȱaȱdayȱorȱtwoȱbeforeȱtheȱsamplingȱ dateȱtoȱensureȱtheyȱareȱinȱaȱsuitableȱlocation.ȱȱ Measuringȱtid alȱm ov e men tȱu s ingȱ st affȱg aug es ȱ Theȱnextȱstepȱisȱtoȱcollectȱtheȱtidalȱrangeȱdata.ȱTheȱstaffsȱareȱmeasuredȱbyȱrecordingȱtheȱlevelȱtheȱwaterȱ reachesȱonȱtheȱstaff.ȱAȱtorchȱorȱpairȱofȱbinocularsȱmayȱbeȱneededȱtoȱhelpȱyouȱseeȱtheȱmarksȱonȱtheȱstaff.ȱȱ Thisȱmonitoringȱrequiresȱsomeoneȱtoȱbeȱonȱsiteȱtoȱrecordȱwaterȱlevelsȱ(tidalȱheightȱinȱAHD)ȱonȱallȱtheȱ staffȱgaugesȱeveryȱ15ȱtoȱ30ȱminutes,ȱoverȱtheȱperiodȱofȱoneȱorȱseveralȱtides.ȱTheȱregularȱreadingsȱareȱ noted,ȱalongȱwithȱtheȱtimeȱofȱeachȱmeasurement,ȱonȱtheȱfieldȱdatasheet.ȱTheȱmonitoringȱisȱusuallyȱdoneȱ overȱaȱperiodȱofȱatȱleastȱsixȱhoursȱsoȱthatȱbothȱaȱhighȱandȱlowȱtideȱisȱcoveredȱforȱtheȱevent.ȱNamesȱofȱ investigators,ȱdateȱofȱsampling,ȱandȱtimeȱofȱhighȱtideȱwillȱalsoȱneedȱtoȱbeȱrecorded.ȱThisȱactivityȱshouldȱ beȱconductedȱatȱthatȱtimeȱofȱtheȱmonthȱwhereȱtheȱtidalȱvariationȱisȱgreatest—theȱmonthlyȱspringȱtides.ȱ Additionalȱsamplingȱatȱtheȱneapȱtidesȱ(theȱperiodȱmidȬmonthȱwhenȱtheȱrangeȱisȱtheȱsmallest)ȱmayȱalsoȱ beȱuseful,ȱbutȱisȱnotȱessential.ȱ Workingȱinȱshiftsȱwithȱdifferentȱpeopleȱtoȱtakeȱtheȱreadingsȱmayȱreduceȱtheȱtimeȱresourcesȱrequiredȱ fromȱeachȱperson.ȱȱ Lessȱintenseȱmonitoringȱmayȱbeȱdoneȱbyȱgoingȱoutȱonȱsiteȱonlyȱatȱhighȱandȱlowȱtides,ȱorȱatȱsetȱtimesȱeachȱ day,ȱandȱtakingȱtheseȱreadingsȱoverȱaȱlongerȱperiodȱofȱtime,ȱperhapsȱsixȱreadingsȱoverȱ12ȱhoursȱorȱtwoȱ readingsȱaȱdayȱforȱaȱweekȱorȱmonth.ȱTheȱintensityȱofȱtheȱmonitoringȱprogramȱdependsȱentirelyȱonȱtheȱ aimsȱofȱtheȱprogram,ȱandȱthisȱwillȱneedȱtoȱbeȱdecidedȱbyȱyourȱresourceȱlimitationsȱandȱinȱconsultationȱ withȱyourȱWaterwatchȱcoordinator.ȱȱ Installing ȱpiezometers ȱ Priorȱtoȱgoingȱoutȱintoȱtheȱfieldȱtoȱinstallȱyourȱpiezometers,ȱitȱisȱimportantȱthatȱyouȱdetermineȱhowȱmanyȱ piezometersȱyouȱwillȱrequire,ȱroughlyȱwhereȱtheyȱwillȱgoȱandȱwhatȱtheȱsurveyedȱgroundȱlevelsȱareȱforȱ thoseȱsites.ȱȱ Siteȱselectionȱisȱbestȱdoneȱduringȱdryȱperiodsȱwhenȱtheȱwetlandȱisȱmostȱaccessible.ȱSelectingȱsitesȱatȱaȱ moderateȱhighȱtideȱwillȱgiveȱyouȱanȱideaȱofȱwhereȱtheȱtideȱcomesȱtoȱinȱyourȱsaltmarsh,ȱandȱallȱ piezometersȱshouldȱbeȱplacedȱaboveȱthisȱmark.ȱȱ Usingȱanȱaerialȱphotograph,ȱdetermineȱtwoȱorȱthreeȱtransectȱlinesȱrunningȱfromȱtheȱhighȱtideȱmarkȱ throughȱtoȱtheȱhighestȱpointȱinȱyourȱsaltmarsh.ȱTheyȱshouldȱformȱaȱstraightȱlineȱperpendicularȱtoȱtheȱ tideȱline,ȱasȱthisȱwillȱhelpȱshowȱtidalȱinfluencesȱonȱgroundwaterȱandȱtheȱzonationȱofȱplants.ȱItȱmayȱbeȱ worthwhileȱplacingȱaȱcoupleȱofȱpiezometersȱinȱaȱreferenceȱsite,ȱwhichȱisȱsimilarȱinȱhabitatȱorȱgeography,ȱ butȱdoesȱnotȱhaveȱtidalȱrestrictions.ȱ Placingȱtheȱtransectsȱatȱdifferentȱdistancesȱfromȱtheȱtidalȱrestriction,ȱforȱexampleȱ50ȱmȱandȱ100ȱm,ȱwillȱ giveȱyouȱanȱideaȱofȱtheȱeffectsȱofȱtidalȱrestrictionȱfurtherȱintoȱtheȱsaltmarsh.ȱPlacingȱyourȱpiezometersȱinȱ differentȱsaltmarshȱhabitatsȱwillȱalsoȱgiveȱyouȱsomeȱinterestingȱandȱusefulȱdata.ȱ 51 ESTUARINE MONITORING GUIDANCE MANUAL Placeȱyourȱtwoȱtoȱfourȱpiezometerȱsitesȱalongȱeachȱtransectȱwithȱatȱleastȱoneȱatȱeachȱend,ȱwithȱanyȱothersȱ spacedȱinȱbetween.ȱExamplesȱofȱsuitableȱpiezometerȱsitesȱareȱshownȱinȱtheȱexampleȱsiteȱplanȱprovidedȱinȱ theȱstaffȱgaugeȱsectionȱabove.ȱȱ Itȱmayȱbeȱadvisableȱtoȱdigȱaȱtestȱholeȱatȱtheȱhighestȱandȱlowestȱpointsȱtoȱworkȱoutȱhowȱlongȱyourȱ piezometersȱwillȱneedȱtoȱbe,ȱsoȱthatȱtheyȱareȱinȱaȱzoneȱofȱgroundwaterȱflow.ȱThisȱisȱdeterminedȱbyȱ diggingȱaȱsmallȱholeȱwithȱaȱshovelȱorȱpostholeȱdiggerȱuntilȱyouȱreachȱaȱpointȱwhereȱtheȱsoilȱisȱsaturatedȱ andȱtheȱbottomȱofȱtheȱholeȱstartsȱtoȱgatherȱwater.ȱAȱmaximumȱdepthȱofȱoneȱmetreȱbelowȱtheȱground,ȱ withȱ300ȱmmȱofȱpipeȱaboveȱgroundȱlevelȱisȱadvised,ȱasȱpiezometersȱlongerȱthanȱthisȱ(1.3ȱm)ȱbecomeȱ difficultȱtoȱhandle.ȱȱ Aȱsurveyorȱorȱsurveyingȱequipmentȱwillȱbeȱneededȱtoȱdetermineȱtheȱheightȱofȱtheȱgroundȱatȱeachȱofȱ yourȱselectedȱpiezometerȱsites.ȱOnceȱeachȱpointȱhasȱbeenȱsurveyed,ȱmarkȱeachȱsiteȱwithȱaȱgardenȱstakeȱ andȱtakeȱaȱGPSȱlocationȱtoȱensureȱthatȱtheȱsiteȱcanȱbeȱfoundȱagain.ȱTheȱpiezometersȱshouldȱthenȱbeȱmadeȱ andȱinstalledȱasȱsoonȱasȱpossible,ȱtoȱminimiseȱtheȱchanceȱthatȱaȱlocationȱwillȱbeȱlost.ȱȱ Theȱpiezometersȱareȱmadeȱbyȱcuttingȱtheȱrequiredȱlengthsȱofȱpipeȱsoȱthatȱatȱleastȱ100–mmȱofȱtheȱpipeȱ willȱbeȱinȱtheȱsaturatedȱzoneȱofȱtheȱsoil.ȱTheȱpipeȱwillȱneedȱtoȱbeȱlongȱenoughȱsoȱthatȱitȱsticksȱoutȱofȱtheȱ soilȱbyȱapproximatelyȱ300ȱmmȱsoȱthatȱtheȱpiezometersȱmayȱbeȱseenȱfromȱaȱdistance,ȱwithoutȱbeingȱ overlyȱobvious.ȱȱ Forȱaȱstandardȱpiezometer,ȱtheȱconstructionȱmethodȱisȱoutlinedȱbelow.ȱ – cutȱaȱoneȬmetreȱsectionȱofȱPVCȱpipeȱ – drillȱsmallȱholesȱ(5ȱmm)ȱapproximatelyȱ20ȱmmȱapartȱintoȱtheȱlowerȱthirdȱofȱtheȱpipeȱ – alternativelyȱaȱhandsawȱcouldȱbeȱusedȱtoȱcutȱslotsȱacrossȱtheȱpipeȱinstead,ȱifȱdrillingȱisȱtooȱtimeȱ consumingȱ – drillȱaȱventȱholeȱ100ȱmmȱfromȱtheȱtopȱofȱtheȱpipeȱ – fitȱanȱendȬcapȱoverȱtheȱtopȱopeningȱofȱtheȱpipe,ȱbeingȱcarefulȱnotȱtoȱcoverȱtheȱventȱholeȱ – fitȱanȱendȬcapȱwithȱaȱfewȱholesȱdrilledȱintoȱitȱoverȱtheȱbottomȱopeningȱofȱtheȱpipeȱ – pullȱatȱleastȱtwoȱstockingsȱoverȱtheȱlowerȱendȱofȱtheȱpipe,ȱincludingȱtheȱopeningȱandȱallȱofȱtheȱ drillȱholesȱ – —ȱȱȱsecureȱtheȱstockingȱfabricȱwithȱductȱtape.ȱ ȱ Installationȱisȱaȱsimpleȱprocedureȱonceȱtheȱsitesȱhaveȱbeenȱ identifiedȱandȱsurveyedȱ(Figureȱ7).ȱAȱpostholeȱaugerȱisȱusedȱtoȱ digȱaȱsmallȱholeȱatȱleastȱ100ȱmmȱacrossȱandȱ50ȱmmȱdeeperȱthanȱ theȱintendedȱdepthȱofȱtheȱpiezometer.ȱȱ Thisȱholeȱisȱfilledȱwithȱapproximatelyȱ50–mmȱofȱcoarseȱgravelȱ orȱsand,ȱthenȱtheȱpiezometerȱisȱplacedȱintoȱtheȱholeȱandȱtheȱ spaceȱaroundȱitȱpackedȱwithȱfurtherȱcoarseȱgravelȱorȱsandȱtoȱ justȱaboveȱtheȱdrillȱholes.ȱTheȱspaceȱaboveȱtheȱdrillȱholesȱisȱ thenȱpackedȱtightȱwithȱbentonite,ȱorȱnativeȱclay,ȱandȱcappedȱ withȱaȱsmallȱamountȱofȱquicksetȱconcreteȱ(ifȱavailable).ȱȱ ȱ Figure 7—Installing a piezometer T h e seȱ sm a llȱg roundw aterȱ monitoring ȱst at ion sȱn eed ȱt oȱ be ȱ l e f tȱt oȱ sett leȱ for ȱa tȱ le a stȱa ȱw ee kȱ be fore ȱth eȱm on it o r ing ȱ c om me nc es. ȱT h eȱd epthȱ ofȱ eachȱp iez om et erȱ fromȱth eȱt opȱ o fȱth eȱ sta nd pi p e, ȱthe ȱh e ig h tȱ o fȱe a chȱ st andp ipe ȱa b ov eȱth eȱ g round ȱ su r fa c eȱ andȱth eȱg round ȱ levelȱ atȱ e achȱ s it e ȱn eed sȱ t oȱ be ȱr ec orde dȱo nȱy ou rȱ fie ld ȱdatasheet.ȱ These ȱw il lȱ b eȱ u sed ȱ la te rȱto ȱh e lp ȱc a lc u l a t e ȱthe ȱgroundwaterȱ lev elȱandȱ w i l lȱh e lpȱy ou ȱrepairȱy ou rȱp iez om ete r sȱ ifȱ anyȱ a re ȱ d amaged. ȱ ȱ 52 ESTUARINE MONITORING GUIDANCE MANUAL Measuringȱg roundw ater ȱ lev e lȱ TheȱfirstȱparameterȱtoȱbeȱmeasuredȱwhenȱdoingȱgroundwaterȱmonitoringȱisȱtheȱStandingȱWaterȱLevelȱ (SWL)ȱofȱtheȱpiezometer.ȱThisȱmayȱbeȱdeterminedȱusingȱaȱvarietyȱofȱequipment.ȱȱ Theȱsimplestȱ(andȱcheapest)ȱwayȱisȱbyȱgentlyȱinsertingȱ(soȱasȱnotȱtoȱdamageȱtheȱbottomȱofȱtheȱwell)ȱaȱ thinȱdryȱwoodenȱorȱbambooȱstakeȱintoȱtheȱpipeȱuntilȱitȱcanȱgoȱnoȱfurther.ȱOnceȱitȱisȱwithdrawnȱtheȱdepthȱ ofȱwaterȱfromȱtheȱbottomȱofȱtheȱwellȱmayȱbeȱmeasuredȱasȱtheȱwetȱsectionȱofȱtheȱstake,ȱorȱtheȱdistanceȱtheȱ wetȱareaȱisȱfromȱtheȱtopȱofȱtheȱstandpipeȱmayȱbeȱmeasured.ȱThisȱmethodȱhasȱitsȱdrawbacks–itȱcanȱbeȱ difficultȱandȱpotentiallyȱdangerousȱcarryingȱlargeȱnumbersȱofȱlongȱstakes,ȱandȱifȱitȱrainsȱonȱthemȱorȱtheyȱ becomeȱwetȱinȱsomeȱotherȱwayȱthereȱisȱnoȱwayȱofȱdeterminingȱwhereȱtheȱwaterȱlevelsȱare.ȱȱ Professionalȱgroundwaterȱmonitoringȱgroupsȱuseȱeitherȱaȱplopperȱorȱbeeper,ȱdependingȱonȱtheȱdepthȱofȱ theȱwellsȱthatȱtheyȱareȱmonitoringȱandȱtheȱfinancialȱresourcesȱofȱtheȱgroup.ȱInstructionsȱforȱmakingȱ ploppersȱandȱbeepersȱareȱincludedȱinȱAppendixȱ6.ȱ AȱplopperȱisȱsimplyȱaȱcupȬshapedȱtinȱobject,ȱwhichȱisȱattachedȱtoȱaȱstringȱandȱloweredȱfaceȱdownȱintoȱ theȱpipe.ȱWhenȱtheȱcupȱhitsȱtheȱwater,ȱtheȱforceȱmakesȱaȱ‘plop’ȱsound.ȱThisȱcanȱbeȱdoneȱseveralȱtimesȱ andȱtheȱaverageȱpointȱwhereȱthisȱsoundȱisȱmadeȱcanȱbeȱmarkedȱonȱtheȱstring,ȱandȱtheȱgroundwaterȱ depthȱisȱcalculatedȱasȱtheȱdepthȱfromȱtheȱtopȱofȱtheȱstandpipe,ȱminusȱtheȱheightȱofȱtheȱstandpipeȱaboveȱ theȱground.ȱȱ Aȱbeeperȱisȱtheȱeasiestȱtoȱuseȱandȱmostȱaccurateȱmethod,ȱparticularlyȱforȱdeepȱpiezometersȱorȱwhereȱtheȱ piezometersȱrequireȱaȱlongȱwalkȱthroughȱtheȱsamphires.ȱItȱcomprisesȱaȱsmallȱelectronicȱcircuit,ȱwhichȱisȱ attachedȱtoȱaȱlong,ȱtwoȬcoredȱelectricalȱcableȱwithȱbothȱtheȱwiresȱexposedȱatȱoneȱend.ȱTheȱcircuitȱisȱ normallyȱanȱopenȱcircuitȱasȱthereȱisȱnothingȱjoiningȱtheȱtwoȱcoresȱofȱtheȱcable.ȱWhenȱtheȱcableȱisȱloweredȱ downȱaȱgroundwaterȱwellȱitȱeventuallyȱcomesȱintoȱcontactȱwithȱwater.ȱWaterȱconductsȱelectricityȱsoȱitȱ completesȱtheȱcircuit.ȱThisȱallowsȱelectricityȱtoȱflowȱbetweenȱtheȱtwoȱwiresȱandȱtheȱbuzzerȱ(heldȱinȱyourȱ hand)ȱbeepsȱandȱaȱlightȱcomesȱon.ȱTheȱwireȱcanȱbeȱjiggledȱupȱandȱdownȱtoȱidentifyȱtheȱexactȱwaterȱlevelȱ fromȱtheȱtopȱofȱtheȱstandpipe.ȱȱ Theȱnextȱstepȱinȱgroundwaterȱmonitoringȱisȱmeasuringȱ theȱpHȱandȱsalinity.ȱToȱdoȱthisȱtheȱcontentsȱofȱtheȱwellȱ needȱtoȱbeȱcompletelyȱremoved,ȱpreferablyȱtoȱtwiceȱtheȱ well’sȱvolume,ȱandȱtheȱwellȱshouldȱbeȱallowedȱtoȱfillȱ againȱtoȱensureȱuncontaminatedȱgroundwaterȱisȱbeingȱ measured.ȱTheȱwellȱmayȱbeȱemptiedȱusingȱaȱbalingȱ device,ȱandȱsomeȱexamplesȱofȱtheseȱareȱshownȱinȱȱȱȱȱȱȱȱȱȱȱ Figureȱ8.ȱ ȱ Figure 8—Assorted groundwater monitoring equipment AȱpetrolȱsiphonȱwithȱaȱoneȬwayȱvalveȱonȱtheȱbaseȱisȱ probablyȱtheȱeasiestȱdeviceȱtoȱobtain.ȱTheȱpipeȱisȱjiggledȱ upȱandȱdownȱwithȱtheȱvalveȱinȱtheȱwater,ȱuntilȱwaterȱ pumpsȱoutȱtheȱtopȱofȱtheȱpipe.ȱContinueȱjigglingȱtheȱpipeȱ upȱandȱdownȱuntilȱnoȱmoreȱwaterȱcomesȱoutȱorȱatȱleastȱ 1.8ȱlitresȱhaveȱbeenȱremovedȱfromȱtheȱstandpipe.ȱȱ Moveȱonȱtoȱtheȱnextȱpiezometer,ȱrinsingȱtheȱbalingȱdeviceȱ withȱdistilledȱwaterȱbeforeȱrepeatingȱtheȱprocess.ȱOnceȱallȱtheȱpiezometersȱhaveȱbeenȱemptied,ȱtheȱfirstȱ oneȱshouldȱhaveȱrecharged.ȱThisȱmayȱtakeȱupȱtoȱanȱhour.ȱReturnȱtoȱtheȱfirstȱpiezometerȱandȱextractȱaȱ 200–300ȱmLȱwaterȱsampleȱfromȱitȱforȱsalinityȱandȱpHȱmeasurement.ȱȱ TestȱyourȱgroundwaterȱsamplesȱinȱaccordanceȱwithȱtheȱguidelinesȱMeasuringȱpHȱandȱMeasuringȱsalinity.ȱ Disposeȱofȱtheȱremainingȱsampleȱbackȱdownȱtheȱpiezometerȱitȱcameȱfrom,ȱandȱcapȱtheȱpiezometer.ȱ RecordȱtheȱsalinityȱandȱpHȱmeasurements.ȱȱ Groundwaterȱlevelȱreadingsȱshouldȱbeȱtakenȱmonthlyȱforȱtheȱdryȱperiodsȱofȱyourȱyear,ȱegȱSeptemberȱ throughȱtoȱApril,ȱwithȱoccasionalȱmonitoringȱduringȱwetterȱmonthsȱifȱaccessȱisȱpossibleȱwithoutȱcausingȱ undueȱdamage.ȱOptimally,ȱsamplesȱfromȱtheȱpiezometersȱwouldȱbeȱcollectedȱforȱsalinityȱandȱpHȱ 53 ESTUARINE MONITORING GUIDANCE MANUAL measurementȱmonthlyȱfromȱOctoberȱthroughȱtoȱMarch.ȱAnȱannualȱorȱbiannualȱreadingȱwouldȱsufficeȱifȱ monitoringȱaȱnaturalȱarea.ȱAdditionalȱcollectionȱofȱgroundwaterȱlevelȱmeasurementsȱatȱhighȱandȱlowȱ tidesȱisȱinformative,ȱasȱlongȱasȱthereȱisȱnoȱsurfaceȱwaterȱonȱtheȱsaltmarshȱnearȱtheȱwells.ȱ Piezometersȱareȱeasilyȱdamagedȱorȱdestroyedȱbyȱfire,ȱfloodsȱorȱvandalism.ȱKeepȱanȱeyeȱonȱyourȱ piezometersȱandȱrepairȱthemȱimmediatelyȱifȱtheyȱareȱdamaged.ȱReplacingȱtheȱcapȱifȱitȱgoesȱmissingȱandȱ ensuringȱthatȱtheȱpiezometersȱareȱkeptȱcappedȱatȱallȱtimesȱwhenȱnotȱbeingȱusedȱisȱimportant.ȱRainȱ contaminationȱofȱtheȱgroundwaterȱmayȱcreateȱsodicȱsoilsȱaroundȱtheȱpiezometerȱifȱtheȱcapȱisȱleftȱoff.ȱȱ I nte rpr et ingȱt id a lȱ movementȱd at a ȱ Bothȱtheȱtideȱandȱgroundwaterȱdataȱneedsȱtoȱbeȱinterpretedȱusingȱgraphsȱandȱcolouredȱmapsȱshowingȱ allȱyourȱresults.ȱTheseȱgraphsȱandȱmapsȱcouldȱbeȱcomparedȱtoȱresultsȱfromȱreferenceȱsitesȱifȱyouȱhaveȱ anyȱavailable.ȱTheȱlocalȱharbourȱauthorityȱmayȱhaveȱlocalȱtidalȱdata,ȱwhileȱtheȱMiningȱBranchȱofȱPIRSAȱ mayȱbeȱableȱtoȱprovideȱinformationȱonȱgroundwaterȱinȱyourȱdistrict.ȱ Whenȱinterpretingȱtidalȱmovementȱdataȱitȱisȱimportantȱtoȱplotȱtheȱdataȱfromȱeachȱsiteȱontoȱaȱgraphȱ showingȱtimeȱandȱdepth.ȱTheseȱgraphsȱcanȱthenȱbeȱcomparedȱtoȱeachȱotherȱtoȱidentifyȱdifferencesȱisȱ shapeȱorȱtidalȱrange.ȱ Anȱunrestrictedȱtidalȱgraphȱwillȱlookȱlikeȱaȱ‘bellȱcurve’ȱorȱ‘sineȱwave’.ȱItȱwillȱbeȱsmoothȱandȱeven.ȱAsȱtheȱ severityȱofȱtheȱtidalȱrestrictionȱincreases,ȱsoȱwillȱtheȱdifferenceȱinȱtidalȱrangeȱandȱshapeȱofȱtheȱgraphȱ betweenȱyourȱunrestrictedȱandȱrestrictedȱsites.ȱȱ Theȱtidalȱrange,ȱorȱtheȱdifferenceȱinȱlevelȱbetweenȱhighȱandȱlowȱtide,ȱisȱoneȱofȱtheȱmostȱimportantȱ ecologicalȱaspectsȱtoȱmonitorȱinȱaȱsaltmarsh.ȱItȱdeterminesȱtheȱareasȱsuitableȱforȱdifferentȱhabitats,ȱsuchȱ asȱseagrass,ȱmangrove,ȱlowȱmarsh,ȱhighȱmarshȱandȱgrassland.ȱȱ Theȱdataȱalsoȱprovideȱtheȱobserverȱwithȱanȱideaȱofȱtheȱtidalȱflushingȱthatȱoccurs.ȱAreasȱthatȱareȱregularlyȱ inundatedȱareȱflushedȱofȱexcessȱsalts,ȱwhereasȱthoseȱthatȱareȱonlyȱoccasionallyȱfloodedȱbecomeȱveryȱ saline.ȱIfȱtheȱareaȱbeingȱstudiedȱhasȱexperiencedȱsoilȱshrinkageȱdueȱtoȱtidalȱrestrictionsȱcausingȱacidȱ sulphateȱsoils,ȱandȱisȱundergoingȱremediation,ȱtheȱstaffȱgaugeȱdataȱcanȱalsoȱindicateȱifȱtooȱmuchȱ tidewaterȱisȱenteringȱtheȱreopenedȱestuary.ȱMangrovesȱorȱotherȱmarineȱemergentȱvegetationȱwillȱrapidlyȱ coloniseȱareasȱthatȱreceiveȱdailyȱinundation.ȱAreasȱthatȱareȱonlyȱinundatedȱaȱcoupleȱofȱtimesȱaȱmonthȱ willȱbeȱmoreȱappropriateȱforȱuplandȱsamphires,ȱwhileȱareasȱthatȱareȱinundatedȱonlyȱseveralȱtimesȱaȱyearȱ areȱlikelyȱtoȱsupportȱsaltbushȱcommunities.ȱ P u t ti n g i t t o g e t h e r Theȱgroundwaterȱdataȱisȱusedȱinȱconjunctionȱwithȱtheȱstaffȱgaugeȱdataȱtoȱdetermineȱifȱsufficientȱtidalȱ flushingȱisȱoccurring.ȱIfȱinsufficientȱtidalȱflushingȱisȱoccurringȱinȱaȱsamphireȱswamp,ȱtheȱfollowingȱtwoȱ scenariosȱcouldȱdevelop:ȱ – inȱaridȱareasȱtheȱgroundwaterȱwillȱshowȱincreasingȱsalinityȱandȱdecreasingȱpHȱoverȱaȱperiodȱofȱ severalȱmonths.ȱAcidȱdischargeȱcouldȱoccurȱafterȱrain.ȱSaltȱscaldsȱmayȱappearȱonȱtheȱsurface,ȱandȱ theȱsaltbushȱcommunityȱwillȱstartȱtoȱinvadeȱtheȱsamphireȱzone.ȱ – Inȱareasȱwhereȱfreshwaterȱcontinuouslyȱdischargesȱintoȱtheȱtidallyȱrestrictedȱareaȱtheȱ groundwaterȱwillȱgraduallyȱbecomeȱfresh.ȱAreasȱofȱsodicȱsoilsȱmayȱappear.ȱGrasslandȱplantsȱmayȱ invadeȱtheȱuplandȱmarshȱandȱreedsȱmayȱinvadeȱtheȱlowerȱmarsh.ȱ Anȱareaȱwithȱsufficientȱflushingȱshouldȱshowȱaȱmonthlyȱsalinityȱcycleȱduringȱneapȱtidesȱandȱdecreasedȱ salinityȱimmediatelyȱafterȱaȱspringȱtide.ȱSeasonalȱvariationsȱwillȱalsoȱbeȱexperiencedȱdependingȱonȱ rainfallȱandȱevaporation.ȱȱ Atȱleastȱ12ȱmonthsȱofȱdataȱneedsȱtoȱbeȱcollectedȱbeforeȱseasonalȱtrendȱinȱ dataȱcanȱbeȱidentified.ȱTheseȱseasonalȱtrendsȱwillȱalsoȱactȱasȱaȱbaseline,ȱ whichȱwillȱallowȱcomparisonsȱwithȱdataȱfromȱotherȱsitesȱorȱoverȱtime.ȱ ȱ 54 E ȱS T U A R I N E MONITORING GUIDANCE MANUAL Chemical assessments ȱ ȱ ȱ ȱ ȱ ȱ ȱ 55 ESTUARINE MONITORING GUIDANCE MANUAL Measuring dissolved oxygen Dissolvedȱoxygenȱ(DO)ȱmeasuresȱtheȱamountȱofȱoxygenȱdissolvedȱ inȱtheȱwater,ȱandȱhenceȱavailableȱtoȱaquaticȱplantsȱandȱanimalsȱforȱ breathing.ȱWhenȱdissolvedȱoxygenȱlevelsȱdrop,ȱaquaticȱlifeȱstartsȱ toȱdie.ȱSomeȱorganismsȱneedȱmoreȱoxygen,ȱandȱsoȱdieȱfirst.ȱFishȱ comingȱtoȱtheȱsurfaceȱforȱairȱisȱoneȱofȱtheȱfirstȱsignsȱofȱlowȱDOȱ levels.ȱȱ Someȱanimalsȱhaveȱadaptationsȱthatȱallowȱthemȱtoȱdealȱwithȱlowȱ dissolvedȱoxygen.ȱMosquitoesȱhaveȱtubesȱtheyȱbreatheȱthrough,ȱ veryȱmuchȱlikeȱsnorkels.ȱOtherȱorganismsȱsuchȱasȱmusselsȱandȱ pipisȱmayȱshutȱtheirȱshellsȱandȱ‘hibernate’.ȱȱ Aȱrangeȱofȱnaturalȱandȱhumanȱrelatedȱfactorsȱaffectsȱtheȱ concentrationȱofȱDOȱinȱwater.ȱNaturalȱfactorsȱincludeȱ temperature,ȱtimeȱofȱdayȱandȱlightȱintensity.ȱHumanȬrelatedȱ factorsȱincludeȱpollutionȱwithȱchemicalsȱthatȱdecreaseȱtheȱlevelsȱofȱ oxygen,ȱincreasedȱnutrientȱloads,ȱandȱtemperatureȱchangesȱthatȱ raiseȱorȱlowerȱtheȱDOȱsaturationȱpoint.ȱȱ Highȱnutrientsȱincreaseȱtheȱgrowthȱofȱalgaeȱinȱtheȱwater.ȱAlgaeȱareȱ plants,ȱusingȱoxygenȱatȱnightȱviaȱrespirationȱandȱcreatingȱoxygenȱ duringȱtheȱdayȱviaȱphotosynthesis.ȱInȱcleanȱwatersȱwithȱlowȱ nutrients,ȱtheȱnightȱtoȱdayȱchangesȱinȱDOȱcontentȱareȱminimal.ȱInȱ nutrientȬrichȱwatersȱtheȱamountȱofȱalgaeȱinȱtheȱwaterȱincreases,ȱ andȱtheȱnight/dayȱDOȱchangeȱmayȱbecomeȱextreme.ȱȱ Oxygenȱmayȱbeȱmeasuredȱusingȱeitherȱaȱdissolvedȱoxygenȱtestȱkitȱ orȱaȱdissolvedȱoxygenȱmeter.ȱTestȱkitsȱareȱcheapȱbutȱtakeȱ considerableȱtime.ȱDissolvedȱoxygenȱmetersȱprovideȱhighlyȱ accurateȱresultsȱandȱtakeȱveryȱlittleȱtime.ȱDOȱmetersȱareȱcommonlyȱȱ soldȱasȱcombinationȱmeters,ȱwhichȱalsoȱmeasureȱpH,ȱtemperatureȱȱ andȱconductivity.ȱTheseȱmetersȱareȱcostlyȱandȱrequireȱregularȱȱ calibrationȱtoȱmaintainȱaccuracy.ȱȱ Activity: 3 Core Age: All Time: 3 Why? Most aquatic organisms require at least 3 – 5 mg/L of dissolved oxygen to survive. The concentration in the water varies depending on salinity, temperature and time of day. Below 3 mg/L indicates hypoxia, and below 0.5 mg/L indicates anoxia. Safety: Dispose of all sample and reagent mixes appropriately. The test kit uses chemicals that can be dangerous if handled incorrectly. Read the instructions provided with the chemicals and equipment. Also check first aid procedures. What you may need: – siteȱrecordȱformȱ – safetyȱglassesȱandȱdisposableȱglovesȱ – sampleȱbottleȱwithȱscrewȱtopȱcapȱȱ – oxygenȱtestȱkitȱ(modifiedȱWinklerȱmethod).ȱ To ensure good results: Checkȱtheȱexpiryȱdatesȱofȱreagentsȱpriorȱtoȱuse.ȱ Getȱtwoȱpeopleȱtoȱeachȱanalyseȱaȱseparateȱsample,ȱandȱaverageȱtheȱresults.ȱ What to do: Rinseȱbottleȱwithȱsampleȱwaterȱandȱsubmergeȱitȱinȱtheȱwaterȱtoȱaboutȱ200–mmȱbelowȱtheȱsurface,ȱuntilȱ filled.ȱWhenȱfull,ȱtapȱtheȱbottleȱsidesȱtoȱremoveȱairȱbubblesȱandȱreplaceȱlidȱwhilstȱstillȱunderȱwater.ȱȱ Immediatelyȱcompleteȱtheȱtestȱprocedureȱasȱoutlinedȱinȱtheȱinstructionsȱprovidedȱwithȱyourȱtestȱkit.ȱBeȱ sureȱtoȱfollowȱsafetyȱinstructionsȱandȱwearȱsafetyȱequipment.ȱRecordȱtheȱresultȱonȱyourȱsiteȱrecordȱformȱ asȱDOȱmg/L.ȱ 56 ESTUARINE MONITORING GUIDANCE MANUAL Bothȱtheȱtemperatureȱandȱtheȱsalinityȱofȱwaterȱaffectȱtheȱsolubilityȱofȱoxygen.ȱSeawaterȱcanȱonlyȱholdȱaȱ littleȱmoreȱthanȱhalfȱtheȱoxygenȱthatȱfreshwaterȱcanȱholdȱatȱtheȱsameȱtemperature.ȱȱ Manyȱgroupsȱstudyingȱestuariesȱreportȱtheȱdissolvedȱoxygenȱcontentȱasȱtheȱpercentȱsaturation,ȱasȱthisȱ takesȱintoȱaccountȱtheȱtemperatureȱandȱsalinityȱeffectsȱandȱgivesȱaȱmoreȱimmediateȱideaȱofȱtheȱhealthȱofȱ yourȱwaterȱbody,ȱinȱcomparisonȱtoȱotherȱwaterȱbodies.ȱToȱcalculateȱtheȱpercentȱsaturationȱyouȱneedȱtoȱ knowȱtheȱtemperatureȱandȱsalinityȱofȱtheȱwater.ȱȱ Valuesȱaboveȱ100%ȱsaturationȱareȱusuallyȱindicativeȱofȱnutrientȱenrichment,ȱoftenȱinȱtheȱformȱofȱsewageȱ orȱfertilisers.ȱTheseȱpollutantsȱcauseȱanȱovergrowthȱinȱplanktonicȱalgaeȱthatȱproduceȱoxygenȱinȱtheȱ daytime.ȱTheȱdownsideȱisȱtheȱamountȱofȱoxygenȱtheyȱuseȱatȱnight,ȱwhenȱtheȱalgaeȱmayȱremoveȱtooȱ muchȱoxygenȱfromȱtheȱwater,ȱkillingȱfish.ȱ Valuesȱbelowȱapproximatelyȱ60%ȱareȱoftenȱindicativeȱofȱorganicȱpollution.ȱBacteriaȱbreakȱdownȱmostȱ organicȱpollutants,ȱandȱtheseȱrequireȱoxygen.ȱThereforeȱorganicȱwastesȱareȱoftenȱdescribedȱbyȱtheirȱ biochemicalȱoxygenȱdemandȱ(BOD).ȱThisȱisȱtheȱamountȱofȱoxygenȱinȱmg/Lȱthatȱaȱsolutionȱofȱtheȱwasteȱ wouldȱdemandȱfromȱtheȱenvironment.ȱ P e r ce n t s a t u ra ti o n ca l cu l a t i o n Ifȱyouȱwouldȱlikeȱtoȱknowȱtheȱapproximateȱpercentȱdissolvedȱoxygenȱsaturationȱofȱyourȱsample,ȱyouȱcanȱ useȱtheȱroughȱconversionȱinȱTableȱ4.ȱAȱmoreȱaccurateȱconversionȱisȱavailableȱusingȱaȱspreadsheet,ȱwhichȱ yourȱWaterwatchȱcoordinatorȱwillȱhave.ȱȱ Toȱundertakeȱthisȱcalculationȱyouȱwillȱneedȱtoȱtestȱsalinity,ȱtemperatureȱandȱdissolvedȱoxygen.ȱTheseȱ willȱneedȱtoȱbeȱreportedȱas;ȱ – salinityȱasȱTDSȱ(g/L)ȱȱ – waterȱtemperatureȱinȱ°Cȱȱ – theȱmeasuredȱDOȱinȱmg/Lȱ(withoutȱtemperatureȱcorrection).ȱȱ Useȱyourȱtemperatureȱandȱsalinityȱreadingsȱtoȱestablishȱtheȱpotentialȱdissolvedȱoxygenȱbasedȱonȱtheȱ tablesȱinȱTchobanoglousȱ&ȱBurtonȱ(1991).ȱȱ Firstȱdetermineȱwhatȱconcentrationȱofȱoxygenȱisȱequivalentȱtoȱ100%ȱsaturationȱinȱyourȱwater.ȱInȱtheȱtableȱ belowȱlocateȱtheȱclosestȱsalinityȱreadingȱacrossȱtheȱtopȱandȱtheȱclosestȱtemperatureȱreadingȱfromȱtheȱleftȱ column.ȱScrollȱacrossȱtheȱrowȱandȱdownȱtheȱcolumnȱtoȱfindȱtheȱpotentialȱsaturationȱoxygenȱcontentȱforȱ yourȱwater.ȱȱ Table 4—Potential saturation oxygen concentrations 0 g/L TDS 0 °C 10 g/L 14.6 20 g/L TDS 30 g/L TDS 13.6 12.7 11.9 40 g/L 11.1 45 g/L 10.7 5 °C 12.8 11.9 11.2 10.5 9.8 9.5 10 °C 11.3 10.6 9.9 9.3 8.8 8.5 15 °C 10.1 9.5 8.9 8.4 7.9 7.7 20 °C 9.1 8.6 8.1 7.6 7.2 7.0 25 °C 8.2 7.8 7.4 7.0 6.6 6.4 30 °C 7.5 7.1 6.8 6.4 6.1 5.9 35 °C 6.9 6.6 6.2 5.9 5.6 5.5 Onceȱyouȱhaveȱdeterminedȱtheȱpotentialȱsaturation,ȱapplyȱtheȱformulaȱbelowȱtoȱcalculateȱtheȱpercentȱ saturationȱforȱyourȱwaterȱbody:ȱȱ Percent saturation = Measured DO Potential DO x 100 57 ESTUARINE MONITORING GUIDANCE MANUAL Measuring sulphide Sulphurȱisȱanȱimportantȱpartȱofȱestuarineȱecosystems.ȱBacteriaȱreduceȱ sulphurȱcompoundsȱtoȱcreateȱenergy.ȱSulphurȱisȱanȱimportantȱ componentȱofȱtheȱheavyȱmetalȱandȱnutrientȱcycles.ȱTheȱoxidisedȱformȱ ofȱsulphur,ȱsulphate,ȱisȱaȱmajorȱcomponentȱsaltȱofȱmarineȱwater.ȱItȱisȱ usuallyȱassociatedȱwithȱcalciumȱandȱmagnesium.ȱȱ Sulphideȱisȱtheȱreducedȱformȱofȱsulphur.ȱItȱisȱregularlyȱpresentȱinȱ lowȱconcentrationȱunderȱtheȱsurfaceȱofȱestuarineȱsediments,ȱbutȱitȱisȱ rarelyȱpresentȱinȱsurfaceȱwater.ȱProblemsȱoccurȱwhenȱsulphideȱ migratesȱintoȱsurfaceȱwater.ȱThisȱchemicalȱisȱhighlyȱreactive,ȱandȱ quicklyȱconsumesȱallȱoxygenȱinȱtheȱwaterȱcolumn.ȱItȱmayȱoxidiseȱintoȱ acid,ȱwhichȱaffectsȱtheȱpHȱofȱtheȱwaterway.ȱȱ Anyȱsulphideȱpresentȱinȱtheȱwaterȱcolumn,ȱparticularlyȱnearȱtheȱ surface,ȱisȱanȱindicatorȱthatȱyourȱwaterwayȱhasȱbeen,ȱisȱorȱisȱaboutȱtoȱ becomeȱanoxicȱ(noȱdissolvedȱoxygen)ȱorȱhypoxicȱ(lowȱinȱoxygen).ȱȱ What you may need: – siteȱrecordȱformȱ – sulphideȱtestȱkitȱ – pHȱstripsȱ – disposableȱglovesȱandȱsafetyȱglassesȱ – liddedȱcontainerȱwithȱkittyȱlitterȱ – sampleȱbottleȱorȱcontainer.ȱ Activity: 3 Extension Age: All Time: 3 Why? The presence of sulphide in the water column may be used as a surrogate for measuring dissolved oxygen. There should be no sulphide present in the free surface water of an estuary. Safety: Sulphide test kits often contain concentrated acid or corrosive chemicals. Use care when handling the reagents, and read the safety instructions provided with the kit. Wear disposable gloves and eye protection. To ensure good results: Sulphideȱfieldȱtestȱkitsȱareȱnotȱasȱpreciseȱorȱaccurateȱasȱlaboratoryȱmethods,ȱbutȱtheȱkitsȱrecommendedȱ byȱWaterwatchȱSAȱareȱaccurateȱenoughȱforȱbasicȱecologicalȱassessment,ȱparticularlyȱwhenȱcombinedȱ withȱdissolvedȱoxygenȱreadings.ȱȱ ManyȱsmallȱtestȱkitsȱhaveȱaȱuseȬbyȱdate.ȱCheckȱyourȱsulphideȱkitȱbeforeȱgoingȱoutȱintoȱtheȱfield.ȱ What to do: Collectȱaȱsampleȱaccordingȱtoȱtheȱsampleȱcollectionȱsectionȱofȱtheseȱguidelines.ȱDoȱnotȱundulyȱshakeȱorȱ aerateȱtheȱsampleȱpriorȱtoȱtestingȱasȱoxygenȱ(presentȱinȱair)ȱmayȱoxidiseȱtheȱsulphideȱinȱtheȱsample.ȱȱ TestȱtheȱpHȱofȱtheȱsample,ȱusingȱtheȱmethodȱoutlinedȱinȱtheȱguidelineȱMeasuringȱpH.ȱRecordȱitȱonȱyourȱ sampleȱsheet.ȱ Theȱsulphideȱtestȱkitȱcomesȱcompleteȱwithȱinstructionsȱonȱhowȱtoȱuseȱit.ȱAsȱkitsȱvaryȱbetweenȱ manufacturers,ȱpleaseȱreadȱtheȱtestȱmethodsȱcarefullyȱandȱfollowȱthemȱasȱcloselyȱasȱyouȱcan.ȱRecordȱtheȱ resultsȱimmediatelyȱonȱyourȱfieldȱdatasheet.ȱ Disposeȱofȱliquidȱwastesȱdirectlyȱintoȱtheȱcontainerȱfilledȱwithȱkittyȱlitter.ȱOnceȱtheȱkittyȱlitterȱisȱfullyȱ used,ȱdisposeȱofȱitȱinȱaȱsealedȱrubbishȱbagȱtoȱgeneralȱwaste.ȱCleanȱallȱequipmentȱwithȱdistilledȱwaterȱ onceȱfinished,ȱasȱyourȱnextȱresultsȱmayȱotherwiseȱbeȱaffected.ȱAllowȱtheȱequipmentȱtoȱairȱdryȱbeforeȱ storingȱit.ȱ Ifȱyouȱcan’tȱunderstandȱyourȱsulphideȱtestȱkitȱinstructions,ȱpleaseȱcontactȱyourȱWaterwatchȱcoordinator.ȱ Theȱsulphideȱconcentrationȱisȱreportedȱasȱmilligramsȱperȱlitreȱofȱsulphideȱ(mg/LȱS2Ȭ).ȱReportȱanyȱpositiveȱ sulphideȱreadingsȱatȱyourȱsiteȱtoȱyourȱWaterwatchȱcoordinator.ȱȱ 58 ESTUARINE MONITORING GUIDANCE MANUAL Measuring orthophosphate Theȱimportanceȱofȱnutrientsȱinȱtheȱestuarineȱenvironmentȱisȱ discussedȱinȱseveralȱsectionsȱofȱthisȱmanual.ȱOrthophosphateȱisȱaȱ solubleȱinorganicȱformȱofȱphosphorus,ȱwhichȱmeansȱthatȱitȱisȱeasilyȱ availableȱtoȱaquaticȱplantsȱandȱanimals.ȱȱ Phosphorusȱ(inȱanyȱofȱitsȱaccessibleȱforms)ȱisȱcriticalȱforȱmetabolicȱ processes,ȱwhichȱinvolveȱtheȱtransferȱofȱenergy.ȱWithoutȱ phosphorusȱanimalsȱandȱplantsȱwouldȱbeȱunableȱtoȱlive.ȱ Phosphorusȱexistsȱinȱtheȱwaterȱinȱseveralȱotherȱforms—mainlyȱ organicȱphosphates,ȱandȱpolyphosphatesȱfromȱdetergents.ȱTotalȱ phosphorusȱcanȱalsoȱbeȱmeasured,ȱbutȱitȱrequiresȱdigestionȱatȱhighȱ temperatures.ȱ Nutrientȱconcentrationsȱvaryȱaccordingȱtoȱsurroundingȱlandȱuse,ȱ seasonȱandȱgeology.ȱNitrogenȱandȱphosphorusȱareȱtwoȱofȱtheȱmostȱ importantȱnutrientsȱforȱtheȱgrowthȱofȱaquaticȱplants.ȱTheirȱ concentrationsȱinȱtheȱenvironmentȱhaveȱincreasedȱdramaticallyȱ sinceȱEuropeanȱsettlement.ȱ Orthophosphateȱinȱwaterȱcomesȱfromȱfertilisersȱandȱisȱtheȱformȱ commonlyȱmeasured,ȱasȱitȱdetectsȱpoorȱagriculturalȱorȱlandȱ managementȱpracticesȱinȱtheȱcatchment.ȱItȱalsoȱindicatesȱsewageȱ pollutionȱfurtherȱfromȱtheȱpointȱofȱdischargeȱthanȱdoesȱnitrogen.ȱ Excessȱphosphatesȱmayȱalsoȱenterȱanȱestuaryȱfromȱsoil,ȱanimalȱ feedlotȱoperationsȱandȱlawns.ȱPhosphatesȱmayȱattachȱtoȱsoilȱ particlesȱandȱbeȱtransportedȱtoȱtheȱestuaryȱwithȱerodedȱsoil.ȱVeryȱ highȱphosphorusȱloadsȱareȱoftenȱdeliveredȱbyȱlargeȱstormwaterȱ dischargesȱorȱfromȱoverȱirrigation.ȱ BlueȬgreenȱalgaeȱbloomsȱareȱlimitedȱbyȱtheȱavailabilityȱofȱ phosphorusȱratherȱthanȱnitrogen,ȱasȱtheyȱcanȱfixȱtheirȱownȱnitrogenȱ fromȱtheȱair.ȱHighȱlevelsȱofȱavailableȱphosphorusȱinȱtheȱwaterȱwillȱ enableȱlargeȱnumbersȱofȱblueȬgreenȱalgaeȱtoȱbloom.ȱSomeȱofȱtheseȱ bloomsȱareȱtoxicȱandȱmayȱcauseȱfishȱkills.ȱ Activity: 3 Core Age: All Time: 2 Why? Total ortho-P includes soluble ortho-P and the more easily reacted particulate P that may be loosely bound to clay particles or incorporated in algae. These forms of P are rapidly taken up by algae. The presence of high concentrations of ortho-P is frequently an indicator of fertiliser or other nutrient runoff. Safety: The orthophosphate test kit contains concentrated chemicals, including acids and alkalis. Use care when handling them, and read the safety instructions provided with the kit. Wear disposable gloves and eye protection. Dispose of all samples and reagents responsibly. Theȱkeyȱtoȱeffectiveȱnutrientȱmonitoringȱisȱtoȱsampleȱasȱoftenȱasȱpossibleȱatȱasȱmanyȱsitesȱasȱpossible,ȱtoȱ accountȱforȱtheȱhighȱvariabilityȱofȱestuarineȱecosystems.ȱSamplingȱduringȱwinterȱmayȱbeȱdecreased,ȱasȱ manyȱaquaticȱplantsȱareȱdormantȱandȱtheȱeffectsȱofȱhighȱphosphorusȱlevelsȱareȱnotȱsoȱobvious.ȱSitesȱmayȱ beȱselectedȱtoȱreflectȱtheirȱproximityȱtoȱaȱdischargeȱlocation,ȱorȱbecauseȱofȱtheirȱrelativelyȱpristineȱnature.ȱȱ Becauseȱnutrientȱlevelsȱoftenȱvaryȱwithȱdepth,ȱespeciallyȱduringȱsummerȱwhenȱtheȱestuaryȱmayȱbeȱ stratified,ȱWaterwatchȱgroupsȱmayȱwishȱtoȱcollectȱsamplesȱatȱdifferentȱdepths.ȱConstructingȱdepthȱ samplersȱisȱdiscussedȱinȱAppendixȱ6.ȱȱ What you may need: – siteȱrecordȱformȱ – orthophosphateȱtestȱkitȱ – containerȱforȱsamplingȱ – fieldȱdatasheetȱ – distilledȱorȱde–ionisedȱwaterȱ – liddedȱcontainerȱfilledȱwithȱkittyȱlitterȱ – safetyȱequipmentȱincludingȱglassesȱandȱdisposableȱgloves.ȱ 59 ESTUARINE MONITORING GUIDANCE MANUAL To ensure good results: Orthophosphateȱfieldȱtestȱkitsȱareȱnotȱasȱpreciseȱorȱaccurateȱasȱmethodsȱusedȱbyȱlaboratories,ȱ particularlyȱwhenȱnutrientȱconcentrationsȱareȱlow.ȱTheȱkitsȱrelyȱonȱaȱcolourȱcomparison:ȱmatchȱtheȱ colourȱofȱaȱpreparedȱwaterȱsampleȱtoȱoneȱonȱaȱcolourȱcard.ȱTheȱsubjectivityȱofȱeachȱvolunteer’sȱdecision,ȱ asȱwellȱasȱambientȱlightȱlevels,ȱmayȱinfluenceȱyourȱresults.ȱDoingȱtestsȱatȱaȱsimilarȱtimeȱofȱday,ȱandȱ averagingȱseveralȱpeople’sȱresultsȱ(duplicateȱanalyses)ȱwillȱensureȱmoreȱconsistentȱresults.ȱ OrthophosphateȱkitȱreagentsȱoftenȱhaveȱaȱuseȬbyȱdate.ȱCheckȱthisȱbeforeȱgoingȱoutȱintoȱtheȱfield.ȱ Theȱphosphateȱtestȱkitsȱ(molybdenumȱblue–acsorbateȱmethod)ȱrecommendedȱbyȱWaterwatchȱforȱuseȱinȱ estuariesȱareȱcompatibleȱwithȱwaterȱupȱtoȱaboutȱtwiceȱseawaterȱsalinity.ȱIfȱtheȱwaterȱyouȱareȱtestingȱisȱ tooȱsalty,ȱitȱwillȱshowȱupȱcloudyȱinȱtheȱsolution.ȱRetestingȱafterȱdilutionȱofȱtheȱsampleȱwithȱdistilledȱ waterȱovercomesȱtheȱproblem.ȱ Recordȱtheȱunitsȱmeasured,ȱandȱtheȱspeciesȱofȱphosphateȱmeasured,ȱonȱyourȱresultsȱsheet.ȱ What to do: Collectȱaȱsampleȱaccordingȱtoȱtheȱinstructionsȱinȱtheȱsampleȱcollectionȱsectionȱofȱthisȱmanual.ȱTestȱtheȱ sampleȱasȱsoonȱasȱpossible.ȱ Theȱorthophosphateȱtestȱkitȱcomesȱwithȱinstructions.ȱAsȱtheȱmethodȱvariesȱbetweenȱmanufacturers,ȱ pleaseȱreadȱtheȱtestȱmethodsȱcarefullyȱandȱfollowȱthemȱasȱcloselyȱasȱyouȱcan.ȱRecordȱtheȱresultsȱ immediatelyȱonȱyourȱfieldȱdatasheet.ȱ Theȱrangeȱofȱorthophosphateȱconcentrationsȱcoveredȱbyȱtheȱkitȱisȱrelativelyȱsmall,ȱinȱorderȱtoȱdetectȱveryȱ smallȱchangesȱinȱtheȱwaterȱquality.ȱIfȱyouȱareȱtestingȱwaterȱthatȱisȱvisiblyȱpolluted,ȱdiluteȱtheȱsampleȱ withȱdeȬionisedȱorȱdistilledȱwaterȱifȱtheȱconcentrationȱisȱhigherȱthanȱthoseȱshownȱonȱtheȱcolourȱcardȱ suppliedȱwithȱtheȱkit.ȱ Disposeȱofȱliquidȱwastesȱdirectlyȱintoȱtheȱcontainerȱfilledȱwithȱkittyȱlitter.ȱOnceȱtheȱkittyȱlitterȱisȱfullyȱ used,ȱdisposeȱofȱitȱinȱaȱsealedȱrubbishȱbagȱtoȱgeneralȱwaste.ȱCleanȱallȱequipmentȱwithȱdistilledȱwaterȱ onceȱfinished,ȱotherwiseȱtheȱcurrentȱresultsȱwillȱaffectȱyourȱnextȱtest.ȱAllowȱtheȱequipmentȱtoȱairȱdryȱ beforeȱstoringȱit.ȱ Ifȱtheȱkitȱinstructionsȱareȱnotȱclear,ȱpleaseȱcontactȱyourȱWaterwatchȱcoordinator.ȱ Theȱinterpretationȱofȱnutrientȱconcentrationȱdataȱmustȱbeȱdoneȱwithȱcare.ȱHighȱnutrientȱconcentrationsȱ oftenȱsuggestȱtheȱpotentialȱforȱexplosiveȱalgalȱgrowth.ȱHowever,ȱlowȱlevelsȱdoȱnotȱnecessarilyȱmeanȱtheȱ estuaryȱisȱreceivingȱlessȱnutrientȱinput.ȱȱ Phytoplanktonȱandȱalgaeȱgrowȱveryȱquickly,ȱsoȱlargeȱquantitiesȱofȱnutrientsȱmayȱhaveȱenteredȱtheȱ estuaryȱandȱhaveȱalreadyȱbeenȱtakenȱupȱbyȱvegetativeȱgrowth.ȱInȱthisȱcase,ȱalthoughȱwaterȱnutrientȱ concentrationsȱareȱlow,ȱtheȱquantityȱofȱnutrientsȱtiedȱupȱinȱbiomassȱisȱhigh.ȱObservationȱofȱplanktonȱ bloomsȱandȱwaterȱclarityȱreadingsȱmayȱhelpȱdetermineȱifȱthisȱisȱtheȱcase.ȱChlorophyllȬaȱanalysisȱorȱ phytoplanktonȱcountsȱmayȱbeȱneededȱifȱyouȱwishȱtoȱquantifyȱtheȱphytoplanktonȱbiomass.ȱ Orthophosphateȱcanȱbeȱexpressedȱasȱmg/Lȱofȱphosphateȱ(PO4)ȱorȱtheȱmassȱofȱelementalȱphosphorusȱperȱ litreȱ(PO4–P).ȱTheȱlatterȱmethodȱisȱtheȱmostȱcommon.ȱCheckȱyourȱkitȱtoȱseeȱwhichȱunitsȱitsȱresultsȱareȱin.ȱȱ AlwaysȱrecordȱtheȱunitȱandȱspeciesȱofȱPȱonȱyourȱrecordȱsheet.ȱIfȱyourȱtestȱkitȱprovidesȱresultsȱasȱȱ mg/LȱPO4,ȱandȱyouȱwouldȱlikeȱtoȱconvertȱtheȱresult,ȱmultiplyȱtheȱresultȱbyȱ0.32ȱtoȱgiveȱyouȱtheȱresultȱinȱȱ mg/LȱPO4–P.ȱ 60 ESTUARINE MONITORING GUIDANCE MANUAL Measuring ammonium Nutrientsȱareȱessentialȱtoȱallȱecosystems.ȱTheyȱareȱtheȱchemicalsȱthatȱ provideȱbuildingȱblocksȱforȱplantȱandȱanimalȱgrowth.ȱAustralianȱ estuariesȱnaturallyȱcontainȱveryȱlowȱlevelsȱofȱnutrients,ȱparticularlyȱ nitrogen.ȱTheseȱlowȱlevelsȱlimitȱplantȱandȱanimalȱgrowth,ȱandȱnativeȱ floraȱandȱfaunaȱhaveȱadaptedȱtoȱlowȱnutrientȱlevels.ȱȱ Manyȱindustrial,ȱagriculturalȱandȱstormwaterȱdischargesȱcontainȱ largeȱamountsȱofȱtheseȱnutrients,ȱwhichȱunbalanceȱourȱfragileȱ estuarineȱecosystems.ȱMeasuringȱtheȱconcentrationȱofȱnutrientsȱgivesȱ usȱanȱindicationȱofȱhowȱbadlyȱaffectedȱtheȱestuaryȱis.ȱ Theȱmostȱcommonlyȱmeasuredȱnutrientsȱareȱphosphorusȱandȱ nitrogen.ȱAmmoniumȱ(NH4+)ȱisȱaȱcompoundȱofȱnitrogen.ȱNitrogenȱ comesȱinȱmanyȱdifferentȱcompounds,ȱdependingȱonȱwhatȱotherȱ chemicalsȱtheȱnitrogenȱatomȱisȱattachedȱto.ȱTheȱmostȱcommonȱformsȱ ofȱnitrogenȱinȱestuariesȱareȱammonium,ȱnitrate,ȱnitrite,ȱureaȱandȱ insolubleȱorganicȱnitrogen.ȱȱ Plantsȱuseȱnitrogenȱinȱphotosynthesis.ȱExcessiveȱnitrogenȱcanȱcauseȱ hugeȱgreenȱalgalȱbloomsȱandȱdinoflagellateȱblooms.ȱHighȱnitrogenȱ levelsȱdoȱnotȱusuallyȱcauseȱblueȬgreenȱalgaeȱblooms,ȱasȱtheseȱ cyanobacteriaȱcanȱfixȱtheirȱownȱnitrogenȱfromȱtheȱairȱorȱwater.ȱHighȱ nutrientȱconcentrationsȱinȱgeneralȱhaveȱbeenȱlinkedȱtoȱharmfulȱorȱ nuisanceȱphytoplanktonȱbloomsȱsuchȱasȱredȱtides,ȱwhichȱcanȱ produceȱharmfulȱtoxins,ȱinȱparticularȱAlexandrinumȱminutum,ȱwhichȱ canȱcauseȱparalyticȱshellfishȱpoisoning.ȱHighȱlevelsȱofȱammoniumȱ mayȱcauseȱfishȱkills.ȱ Activity: 3 Core Age: All Time: 2 Why? High levels of ammonium are toxic to fish. SA EPA considers <0.05 mg/L indicates good water quality for ammonium in an estuary, and that 0.5 mg/L indicates poor water quality. Safety: The ammonium test kit contains concentrated chemicals, including acids and alkalis. Use care when handling them, and read the safety instructions provided with the kit. Wear disposable gloves and eye protection. It is important to use a nonNessler test, as Nessler’s reagent contains mercury. Dispose of all sample and Measuringȱtheȱconcentrationȱofȱtotalȱnitrogenȱisȱdifficult,ȱasȱitȱ reagent mixes requiresȱdigestionȱatȱhighȱtemperaturesȱandȱaȱlotȱofȱfairlyȱexpensiveȱ appropriately. laboratoryȱequipment.ȱManyȱcommunityȱgroupsȱandȱscientificȱ surveysȱmeasureȱtheȱindividualȱformsȱofȱnitrogenȱinstead,ȱasȱsomeȱofȱ theseȱareȱsimplerȱtoȱmeasureȱandȱtheyȱmayȱgiveȱindicationsȱofȱtheȱsourceȱofȱanyȱexcessȱnitrogen.ȱȱ Nutrientȱconcentrationȱvariesȱaccordingȱtoȱsurroundingȱlandȱuse,ȱseasonȱandȱgeology.ȱTheȱtidalȱflushingȱ ofȱtheȱestuary,ȱwaterȱtemperature,ȱbiologicalȱactivityȱandȱsurroundingȱlandȱmanagementȱmethodsȱmayȱ alsoȱhaveȱanȱeffect.ȱȱ Ammoniumȱwasȱselectedȱforȱthisȱactivityȱasȱitȱisȱeasyȱtoȱmeasureȱinȱseawaterȱandȱisȱindicativeȱofȱlowȱ oxygenȱlevels,ȱsewerageȱoutfalls,ȱanimalȱfeedlotȱeffluentsȱorȱcausticȱplantȱoutfalls.ȱTheseȱpollutionȱ sourcesȱcommonlyȱdischargeȱintoȱSouthȱAustralianȱestuaries.ȱȱ Theȱkeyȱtoȱeffectiveȱnutrientȱmonitoringȱisȱtoȱsampleȱasȱoftenȱasȱpossibleȱatȱasȱmanyȱsitesȱasȱpossibleȱtoȱ accountȱforȱtheȱhighȱvariabilityȱofȱestuarineȱecosystems.ȱSamplingȱduringȱwinterȱmayȱbeȱdecreasedȱasȱ manyȱaquaticȱplantsȱareȱdormantȱandȱtheȱeffectsȱofȱhighȱnitrogenȱlevelsȱareȱnotȱsoȱobvious.ȱSitesȱmayȱbeȱ selectedȱbasedȱonȱtheirȱproximityȱtoȱdischargeȱlocationsȱorȱbecauseȱofȱtheirȱrelativelyȱpristineȱnature.ȱȱ Becauseȱnutrientȱlevelsȱoftenȱvaryȱwithȱdepth,ȱespeciallyȱduringȱtheȱsummerȱwhenȱtheȱestuaryȱmayȱbeȱ stratified,ȱWaterwatchȱgroupsȱmayȱwishȱtoȱcollectȱsamplesȱatȱdifferentȱdepths.ȱConstructingȱdepthȱ samplersȱisȱdiscussedȱinȱAppendixȱ6.ȱȱ 61 ESTUARINE MONITORING GUIDANCE MANUAL What you may need: – siteȱrecordȱformȱ – ammoniumȱtestȱkitȱ – containerȱforȱsamplingȱ – distilledȱorȱdeionisedȱwaterȱ – liddedȱcontainerȱfilledȱwithȱkittyȱlitterȱ – safetyȱequipmentȱincludingȱglassesȱandȱdisposableȱgloves.ȱ To ensure good results: Ammoniumȱfieldȱtestȱkitsȱareȱnotȱasȱpreciseȱorȱasȱaccurateȱasȱmanyȱotherȱmethodsȱusedȱbyȱlaboratories,ȱ particularlyȱwhenȱnutrientȱlevelsȱareȱlow.ȱGetȱtheȱbestȱresultsȱpossibleȱbyȱobservingȱtheȱfollowingȱ recommendations.ȱ Ammoniumȱkitsȱhaveȱaȱuse–byȱdate.ȱCheckȱthisȱbeforeȱgoingȱoutȱintoȱtheȱfield.ȱFreshȱreagentsȱareȱanȱ essentialȱpartȱofȱaccurateȱresults.ȱ Theȱkitsȱrelyȱonȱaȱcolourȱcomparison:ȱmatchȱtheȱcolourȱofȱaȱpreparedȱwaterȱsampleȱtoȱaȱcolourȱonȱaȱ colourȱcard.ȱTheȱsubjectivityȱofȱeachȱvolunteer’sȱdecision,ȱasȱwellȱasȱtheȱambientȱlightȱlevels,ȱwillȱ influenceȱtheȱresultsȱtoȱsomeȱdegree.ȱDoingȱtestsȱatȱaȱsimilarȱtimeȱofȱday,ȱandȱgettingȱseveralȱpeopleȱtoȱ testȱsamplesȱ(duplicateȱanalysis)ȱwillȱensureȱmoreȱreliableȱresults.ȱ Recordȱtheȱunitsȱmeasuredȱandȱtheȱspeciesȱofȱnitrogenȱmeasuredȱonȱyourȱresultsȱsheet.ȱ What to do: Collectȱaȱsampleȱaccordingȱtoȱtheȱinstructionsȱinȱtheȱsampleȱcollectionȱsectionȱofȱthisȱmanual.ȱDoȱnotȱ shakeȱorȱaerateȱtheȱsampleȱpriorȱtoȱtestingȱasȱammoniumȱrapidlyȱoff–gasses.ȱTestȱtheȱsampleȱasȱsoonȱasȱ possible.ȱ Theȱammoniumȱtestȱkitȱcomesȱcompleteȱwithȱinstructionsȱonȱhowȱtoȱuseȱit.ȱAsȱtestȱkitȱmethodsȱvaryȱ betweenȱmanufacturers,ȱpleaseȱreadȱtheȱtestȱmethodsȱcarefullyȱandȱfollowȱthemȱasȱcloselyȱasȱyouȱcan.ȱ Recordȱtheȱresultsȱimmediatelyȱonȱyourȱfieldȱdatasheet.ȱ Theȱrangeȱofȱammoniumȱconcentrationsȱcoveredȱbyȱtheȱkitȱisȱrelativelyȱsmall,ȱasȱveryȱlowȱconcentrationsȱ affectȱtheȱenvironment.ȱIfȱyouȱareȱtestingȱnearȱaȱpollutedȱoutfall,ȱdiluteȱtheȱsampleȱwithȱde–ionisedȱorȱ distilledȱwaterȱifȱtheȱammoniumȱconcentrationȱisȱhigherȱthanȱthatȱshownȱonȱtheȱcolourȱcardȱsuppliedȱ withȱtheȱkit.ȱ Disposeȱofȱliquidȱwastesȱdirectlyȱintoȱtheȱcontainerȱfilledȱwithȱkittyȱlitter.ȱOnceȱtheȱkittyȱlitterȱisȱfullyȱ used,ȱdisposeȱofȱitȱinȱaȱsealedȱrubbishȱbagȱtoȱgeneralȱwaste.ȱCleanȱallȱequipmentȱwithȱdistilledȱwaterȱ onceȱfinished,ȱotherwiseȱtheȱcurrentȱresultsȱwillȱaffectȱyourȱnextȱtest.ȱAllowȱtheȱequipmentȱtoȱairȱdryȱ beforeȱstoringȱit.ȱ Ifȱtheȱkitȱinstructionsȱareȱnotȱclear,ȱpleaseȱcontactȱyourȱWaterwatchȱcoordinator.ȱ Theȱinterpretationȱofȱnutrientȱconcentrationȱdataȱmustȱbeȱdoneȱwithȱcare.ȱHighȱnutrientȱconcentrationsȱ oftenȱsuggestȱtheȱpotentialȱforȱexplosiveȱalgalȱgrowth;ȱlowȱlevelsȱdoȱnotȱnecessarilyȱmeanȱtheȱestuaryȱisȱ receivingȱlessȱnutrientȱinput.ȱPhytoplanktonȱandȱalgaeȱgrowȱveryȱquickly,ȱsoȱlargeȱquantitiesȱofȱ nutrientsȱmayȱhaveȱcomeȱintoȱtheȱestuaryȱandȱhaveȱalreadyȱbeenȱtakenȱupȱbyȱvegetativeȱgrowth.ȱȱ Inȱthisȱcase,ȱalthoughȱwaterȱnutrientȱconcentrationsȱareȱlow,ȱtheȱquantityȱofȱnutrientsȱtiedȱupȱinȱbiomassȱ isȱhigh.ȱObservationsȱofȱplanktonȱbloomsȱandȱwaterȱclarityȱreadingsȱwillȱhelpȱdetermineȱifȱthisȱisȱtheȱ case.ȱChlorophyll–aȱanalysisȱorȱphytoplanktonȱcountsȱareȱneededȱifȱyouȱwishȱtoȱquantifyȱtheȱ phytoplanktonȱbiomass.ȱ 62 ESTUARINE MONITORING GUIDANCE MANUAL Measuring chlorophyll-a PhytoplanktonȱareȱautotrophicȱsingleȬcelledȱmicroȬorganismsȱthatȱ liveȱinȱwater.ȱPhytoplanktonȱoccurȱnaturallyȱinȱallȱwatersȱofȱtheȱ worldȱthatȱareȱregularlyȱexposedȱtoȱsunlight.ȱTheȱabundanceȱofȱtheseȱ organismsȱdependsȱonȱtheȱamountȱofȱlightȱtheyȱreceiveȱandȱtheȱ quantityȱofȱnutrientsȱinȱtheȱwater.ȱȱ Anȱincreaseȱinȱtheȱconcentrationȱofȱnutrientsȱ(includingȱcarbon,ȱsilica,ȱ phosphateȱandȱnitrogen)ȱinȱtheȱwaterȱrapidlyȱcausesȱanȱincreaseȱinȱ theȱpopulationȱofȱphytoplankton.ȱTherefore,ȱtheirȱpresenceȱandȱ biomassȱisȱanȱindicatorȱofȱtheȱnutrientȱloadȱinȱtheȱestuary.ȱTheȱ biomassȱofȱphytoplanktonȱcanȱbeȱdeterminedȱbyȱidentifyingȱthemȱ andȱcountingȱthemȱunderȱaȱmicroscope.ȱThisȱtakesȱexpensiveȱ equipmentȱandȱconsiderableȱexperience.ȱChlorophyllȬaȱisȱtheȱgreenȱ pigmentȱusedȱbyȱplantsȱduringȱphotosynthesis.ȱMeasuringȱ chlorophyllȬaȱisȱanotherȱwayȱofȱestimatingȱbiomass,ȱasȱthereȱisȱaȱ wellȬacceptedȱrelationshipȱbetweenȱchlorophyll–aȱandȱbiomass.ȱȱ Activity: 3 Extension Age: Adult groups Time: 4 Why? Chlorophyll is the green pigment in plants and so is a good measure of the amount of plankton present in the water, and therefore reflects the trophic status of the estuary. This is a surrogate test for nutrients. ToȱmeasureȱchlorophyllȬa,ȱaȱsampleȱofȱwaterȱisȱfiltered,ȱtoȱtrapȱallȱtheȱphytoplanktonȱonȱtheȱfilterȱpad.ȱInȱ theȱlaboratoryȱchemicalsȱareȱusedȱtoȱdestroyȱtheȱcellsȱandȱdissolveȱtheȱchlorophyllȬa.ȱThisȱisȱthenȱputȱ intoȱaȱspectrophotometerȱtoȱdetermineȱtheȱconcentrationȱofȱtheȱpigment.ȱMostȱgroupsȱmonitoringȱthisȱ parameterȱneedȱtoȱsendȱtheirȱsampleȱawayȱtoȱaȱspecialisedȱlaboratory,ȱasȱthereȱisȱnoȱwayȱtoȱdoȱthisȱinȱtheȱ field.ȱ Thisȱguidelineȱoutlinesȱtheȱmethodȱrequiredȱtoȱcollect,ȱstoreȱandȱprepareȱaȱchlorophyllȬaȱsampleȱforȱ laboratoryȱanalysis.ȱ What you may need: – siteȱrecordȱformȱ – twoȬlitreȱsamplingȱbottleȱwithȱlidȱ – 500ȬmLȱmeasuringȱcontainerȱȱ – aȱvacuumȱpumpȱwithȱgaugeȱforȱuseȱinȱtheȱfield,ȱorȱaȱsampleȱlocationȱcloseȱtoȱaȱlaboratoryȱwithȱaȱ suitableȱpumpȱȱ – 500ȬmLȱvacuumȱflaskȱtoȱfitȱgaugeȱȱ – 47Ȭmmȱglassȱfibreȱ(GF/C)ȱfilterȱpaperȱȱ – forcepsȱ – sealableȱairtight,ȱlabelledȱsmallȱplasticȱcontainers–eachȱcontainerȱneedsȱtoȱbeȱlabelled,ȱwithȱspaceȱ toȱrecordȱtheȱsiteȱlocation,ȱdateȱandȱtimeȱofȱcollectionȱandȱamountȱofȱwaterȱfilteredȱȱ – aluminiumȱfoilȱtoȱwrapȱsmallȱcontainersȱinȱ – smallȱplasticȱsnapȱlockȱbagsȱforȱsmallȱcontainersȱȱ – oneȱsmallȱeskyȱwithȱiceȱorȱrapidȱaccessȱtoȱaȱfreezer.ȱ To ensure good results: Doȱnotȱexceedȱ400Ȭmmȱmercuryȱvacuumȱwhenȱpumpingȱasȱchlorophyllȱmayȱbeȱforcedȱthroughȱtheȱfilter.ȱ Filterȱtheȱsampleȱandȱfreezeȱitȱasȱsoonȱasȱpossible.ȱ Itȱisȱeasierȱtoȱcentreȱtheȱfilterȱpaperȱonȱtheȱfilterȱholderȱifȱtheȱfilterȱisȱslightlyȱwet.ȱ Itȱisȱbestȱtoȱfilterȱasȱmuchȱwaterȱasȱpossibleȱ(upȱtoȱtwoȱlitres).ȱHowever,ȱitȱisȱalsoȱimportantȱnotȱtoȱclogȱ theȱfilter,ȱasȱallȱwaterȱinȱtheȱfilteringȱapparatusȱmustȱbeȱfiltered.ȱTheȱbestȱwayȱtoȱmakeȱsureȱofȱthisȱisȱtoȱ onlyȱputȱaȱsmallȱamountȱofȱwaterȱintoȱtheȱfilteringȱreservoirȱatȱaȱtime.ȱIfȱtheȱfilterȱstartsȱtoȱclogȱ(theȱflowȱ 63 ESTUARINE MONITORING GUIDANCE MANUAL ofȱwaterȱthroughȱtheȱfilterȱslowsȱdown),ȱceaseȱfilteringȱasȱsoonȱasȱtheȱsmallȱamountȱyouȱhaveȱinȱtheȱfilterȱ reservoirȱhasȱfiltered,ȱandȱrecordȱtheȱvolumeȱfilteredȱonȱyourȱdatasheet.ȱȱ Ifȱtheȱfilterȱbecomesȱcompletelyȱcloggedȱbeforeȱallȱtheȱwaterȱinȱtheȱfilteringȱcontainerȱcanȱbeȱfiltered,ȱtheȱ testȱwillȱneedȱtoȱbeȱredone.ȱȱ TheȱquantityȱofȱchlorophyllȬaȱpresentȱmayȱbeȱreportedȱbyȱtheȱlaboratoryȱinȱseveralȱforms;ȱusuallyȱitȱisȱinȱ Pg/Lȱ(microgramsȱperȱlitre),ȱbutȱitȱisȱwiseȱtoȱcheckȱcarefully.ȱȱ Someȱlaboratoriesȱmayȱpreferȱthatȱtheȱsampleȱisȱgivenȱtoȱthemȱinȱanȱunfilteredȱform;ȱifȱso,ȱaskȱthemȱhowȱ theȱsampleȱisȱtoȱbeȱhandledȱandȱhowȱquicklyȱtheȱsampleȱmustȱreachȱtheȱlaboratory.ȱ What to do: CollectȱaȱtwoȬlitreȱsampleȱfromȱtheȱwaterȱbodyȱ200ȱmmȱbelowȱtheȱsurface.ȱPourȱ500ȬmLȱofȱtheȱsampleȱ intoȱtheȱmeasuringȱflask,ȱreadyȱforȱfiltering.ȱ Placeȱtheȱfilterȱpaperȱinȱtheȱfilter,ȱensuringȱthatȱtheȱwrinkledȱsideȱofȱtheȱpaperȱfacesȱupwards.ȱPlaceȱ250Ȭ mLȱfromȱtheȱmeasuringȱflaskȱintoȱtheȱtopȱofȱtheȱfilterȱdeviceȱandȱuseȱtheȱpumpȱtoȱcreateȱaȱvacuumȱinȱtheȱ flask.ȱKeepȱtheȱwaterȱlevelȱaboveȱtheȱfilterȱdeviceȱsemiȬfullȱusingȱwaterȱfromȱtheȱ500ȬmLȱflask,ȱbutȱbeȱ carefulȱnotȱtoȱblockȱtheȱfilter.ȱȱ Onceȱtheȱ500ȬmLȱflaskȱhasȱbeenȱemptied,ȱrefillȱtoȱtheȱ500ȬmLȱlineȱandȱcontinueȱfiltering.ȱAfterȱallȱwaterȱ hasȱbeenȱfiltered,ȱorȱtheȱfilterȱhasȱstartedȱtoȱblockȱup,ȱandȱtheȱfilteringȱdeviceȱisȱempty,ȱleaveȱtheȱfilterȱinȱ placeȱforȱaȱseveralȱsecondsȱtoȱensureȱtheȱfilterȱpaperȱhasȱbeenȱsuckedȱdry.ȱ Recordȱtheȱvolumeȱofȱwaterȱthatȱwasȱfiltered.ȱRemoveȱtheȱfilterȱpaperȱfromȱtheȱfilterȱscreenȱgentlyȱusingȱ theȱforceps,ȱensuringȱthatȱtheȱupperȱsurfaceȱofȱtheȱfilterȱpaperȱ(whichȱcontainsȱtheȱchlorophyllȬa)ȱ remainsȱuntouched.ȱFoldȱtheȱpaperȱ(upperȱsurfaceȱonȱtheȱinside)ȱoverȱtwiceȱthenȱplaceȱintoȱaȱsmall,ȱ labelledȱcontainer.ȱȱ Recordȱtheȱsiteȱlocation,ȱdate,ȱtimeȱsampled,ȱandȱvolumeȱofȱwaterȱfilteredȱonȱtheȱlabelȱandȱonȱyourȱ datasheet.ȱ WrapȱtheȱcontainersȱcompletelyȱwithȱaluminiumȱfoilȱtoȱstopȱlightȱfromȱdegradingȱtheȱchlorophyllȬaȱthenȱ placeȱintoȱaȱplasticȱbagȱinȱtheȱeskyȱorȱfreezer.ȱFreezeȱtheȱsamplesȱimmediately.ȱȱ Arrangeȱtoȱtransferȱtheȱsamplesȱfromȱtheȱstorageȱareaȱtoȱtheȱlaboratoryȱthatȱwillȱcompleteȱtheȱanalysis.ȱ Frozenȱsamplesȱmayȱbeȱkeptȱinȱstorageȱforȱupȱtoȱaȱmonthȱbeforeȱbeingȱtransportedȱtoȱtheȱlaboratoryȱforȱ analysis,ȱalthoughȱtheȱtemperatureȱmustȱnotȱexceedȱ0°C.ȱȱ ChlorophyllȬaȱconcentrationsȱvaryȱdependingȱonȱtheȱtimeȱofȱyearȱandȱweatherȱconditions.ȱPlanktonȱ countsȱ(andȱthereforeȱchlorophyll)ȱtendȱtoȱbeȱhigherȱduringȱsunnyȱwarmȱmonthsȱandȱlowerȱduringȱcoldȱ darkȱmonths.ȱRecordingȱtemperature,ȱrecentȱweatherȱandȱdateȱofȱsamplingȱisȱimportantȱwhenȱtakingȱ chlorophyllȬaȱreadings.ȱȱ Phytoplanktonȱdoȱnotȱgrowȱsoȱwellȱinȱwaterȱwithȱlowȱclarityȱasȱtheyȱrequireȱsunlight.ȱThereforeȱtakingȱ Secchiȱdepthȱorȱturbidityȱreadingsȱatȱtheȱsameȱtimeȱasȱtheȱsampleȱisȱcollectedȱisȱadvisable.ȱAnotherȱ variableȱthatȱinfluencesȱtheȱquantityȱofȱphytoplanktonȱcellsȱinȱtheȱwaterȱisȱtheȱamountȱofȱflowȱorȱtidalȱ flushing.ȱAreasȱwithȱlowȱflowȱorȱflushingȱwillȱhaveȱhigherȱreadingsȱthanȱotherȱareas.ȱ 64 ESTUARINE MONITORING GUIDANCE MANUAL Measuring alkalinity Totalȱalkalinityȱ(alsoȱknownȱasȱbufferȱcapacity)ȱmeasuresȱtheȱ capacityȱofȱwaterȱtoȱneutraliseȱacids.ȱAlkalineȱcompoundsȱsuchȱasȱ bicarbonates,ȱcarbonates,ȱandȱhydroxidesȱremoveȱhydrogenȱionsȱandȱ lowerȱtheȱacidityȱofȱtheȱwaterȱ(therebyȱincreasingȱpH).ȱȱ Alkalinity,ȱacidity,ȱpHȱandȱhardnessȱareȱallȱrelatedȱandȱareȱpartȱofȱ theȱcarbonȱcycleȱofȱtheȱestuary.ȱTotalȱalkalinityȱisȱmeasuredȱbyȱ measuringȱtheȱamountȱofȱacidȱ(forȱexample,ȱsulphuricȱacid)ȱneededȱ toȱbringȱtheȱsampleȱtoȱaȱpHȱofȱ4.2.ȱAtȱthisȱpH,ȱallȱtheȱalkalineȱ compoundsȱinȱtheȱsampleȱareȱusedȱup.ȱȱ Manyȱthings,ȱsuchȱasȱtheȱsurroundingȱsoil,ȱsaltsȱleachingȱintoȱtheȱ waterway,ȱplantȱgrowthȱandȱindustrialȱwastewaterȱdischarges,ȱ influenceȱtheȱalkalinityȱofȱanȱestuary.ȱ Alkalinityȱisȱimportantȱforȱdeterminingȱtheȱestuary’sȱabilityȱtoȱ neutraliseȱacidicȱpollutionȱfromȱrainfall,ȱacidȱgroundwaterȱorȱ wastewater.ȱTheȱmeasureȱgivesȱenvironmentalȱmanagersȱanȱ indicationȱofȱtheȱchemicalȱmakeȬupȱofȱtheȱwaterȱcontainedȱinȱyourȱ estuaryȱandȱanyȱlongȬtermȱtrendsȱthatȱmayȱbeȱoccurring.ȱȱ Thisȱbufferingȱcapacityȱofȱwater,ȱorȱitsȱabilityȱtoȱresistȱpHȱchange,ȱisȱ criticalȱtoȱaquaticȱlife.ȱTheȱestuary’sȱcapacityȱtoȱneutraliseȱacidsȱwillȱ varyȱbetweenȱtheȱfreshwaterȱreachesȱofȱtheȱestuaryȱandȱtheȱportionsȱ withȱhigherȱsalinity.ȱ Activity: 3 Core Age: All Time: 2 Why? Alkalinity measures the water’s ability to neutralise acidity. Fresh water varies from 30 to 90 mg/L CaCO3, while seawater has around 116 mg/L. Safety: The alkalinity test kit contains concentrated acid. Use care when handling the reagents, and read the safety instructions provided with the kit. Wear disposable gloves and eye protection. Dispose of all reagent/sample mixes appropriately. What you may need: – siteȱrecordȱformȱ – alkalinityȱtestȱkitȱ – disposableȱglovesȱandȱsafetyȱglassesȱ – liddedȱcontainerȱwithȱkittyȱlitterȱ – sampleȱbottleȱorȱcontainer.ȱ To ensure good results: Alkalinityȱtestȱkitsȱareȱnotȱasȱpreciseȱorȱaccurateȱasȱmethodsȱusedȱinȱlaboratories,ȱparticularlyȱasȱtheȱ dropsȱneedȱtoȱbeȱcountedȱmanually,ȱthereforeȱduplicateȱanalysesȱareȱrecommended.ȱ Alkalinityȱkitsȱhaveȱaȱuse–byȱdate.ȱCheckȱthisȱbeforeȱgoingȱoutȱintoȱtheȱfield.ȱ What to do: Collectȱaȱsampleȱaccordingȱtoȱtheȱinstructionsȱprovidedȱinȱtheȱsampleȱcollectionȱsectionȱofȱthisȱmanual.ȱ Doȱnotȱundulyȱshakeȱorȱaerateȱtheȱsampleȱpriorȱtoȱtestingȱasȱcarbonȱdioxideȱ(presentȱinȱair)ȱhasȱanȱeffectȱ onȱalkalinity.ȱȱ Theȱalkalinityȱtestȱkitȱcomesȱcompleteȱwithȱinstructionsȱonȱhowȱtoȱuseȱit.ȱAsȱtheȱmethodȱmayȱvaryȱ betweenȱmanufacturers,ȱpleaseȱreadȱtheȱtestȱmethodsȱcarefullyȱandȱfollowȱthemȱasȱcloselyȱasȱyouȱcan.ȱ Recordȱtheȱresultsȱimmediatelyȱonȱyourȱfieldȱdatasheet.ȱ Disposeȱofȱliquidȱwastesȱdirectlyȱintoȱtheȱcontainerȱfilledȱwithȱkittyȱlitter.ȱOnceȱtheȱkittyȱlitterȱisȱfullyȱ used,ȱdisposeȱofȱitȱinȱaȱsealedȱrubbishȱbagȱtoȱgeneralȱwaste.ȱCleanȱallȱequipmentȱwithȱdistilledȱwaterȱ onceȱfinished;ȱotherwiseȱtheȱcurrentȱresultsȱwillȱaffectȱyourȱnextȱtest.ȱAllowȱtheȱequipmentȱtoȱairȱdryȱ beforeȱstoringȱit.ȱ Ifȱtheȱkitȱinstructionsȱareȱnotȱclear,ȱpleaseȱcontactȱyourȱWaterwatchȱcoordinator.ȱȱ 65 ESTUARINE MONITORING GUIDANCE MANUAL Theȱresultsȱareȱreportedȱasȱmilligramsȱperȱlitreȱofȱcalciumȱcarbonateȱ(mg/LȱCaCO3).ȱ AȱhighȱalkalinityȱreadingȱdoesȱnotȱmeanȱthatȱtheȱwaterwayȱisȱalkalineȱorȱhasȱaȱhighȱpH.ȱSomeȱwaterȱcanȱ testȱasȱacid,ȱorȱhaveȱaȱlowȱpH,ȱyetȱstillȱhaveȱaȱhighȱalkalinity.ȱThisȱmeansȱthatȱwhileȱtheȱwaterȱmightȱbeȱ acidic,ȱitȱstillȱhasȱaȱcapacityȱtoȱbufferȱorȱneutralise,ȱacids.ȱ AȱlowȱbufferingȱabilityȱmeansȱthatȱtheȱpHȱofȱtheȱwaterȱwillȱfluctuateȱconsiderably,ȱandȱonlyȱplantsȱorȱ animalsȱthatȱcanȱtolerateȱlargeȱpHȱrangesȱwillȱsurvive.ȱȱ Totalȱalkalinityȱinȱseawaterȱaveragesȱ116ȱmg/L.ȱThisȱisȱgreaterȱthanȱthatȱofȱfreshwater,ȱwhichȱrangesȱ fromȱ30ȱtoȱ90ȱmg/Lȱdependingȱonȱtheȱwatershed.ȱEstuarineȱwaterȱshouldȱhaveȱaȱtotalȱalkalinityȱbetweenȱ theseȱvalues.ȱ ȱ ȱ ȱ 66 ESTUARINE MONITORING GUIDANCE MANUAL Measuring total suspended solids Suspendedȱsolidsȱinȱwaterȱareȱtheȱsolidsȱthat,ȱonceȱpickedȱupȱbyȱtheȱ water,ȱdoȱnotȱsettleȱout.ȱTheyȱareȱveryȱfineȱparticlesȱofȱorganicȱ matter,ȱpollutantsȱandȱsoilȱthatȱareȱheldȱsuspendedȱinȱtheȱwaterȱ column.ȱTheseȱparticlesȱinterfereȱwithȱwaterȱclarity,ȱcausingȱturbidityȱ andȱblockingȱtheȱpassageȱofȱlightȱtoȱtheȱanimalsȱandȱplantsȱinȱtheȱ benthicȱzone.ȱȱ Highȱloadingsȱofȱsuspendedȱsolidsȱcanȱcauseȱstarvationȱinȱfilterȱ feedersȱsuchȱasȱmusselsȱandȱseaȱanemones,ȱasȱwellȱasȱblockingȱtheȱ gillsȱofȱfish.ȱ Suspendedȱsolidsȱareȱmeasuredȱbyȱfilteringȱaȱsampleȱthroughȱaȱfineȱ glassȱfibreȱfilterȱ(GDȱ120),ȱwhichȱcapturesȱallȱsolidsȱoverȱ2.7ȱΐmȱ (2.7ȱmicron)ȱandȱallowsȱfinerȱparticulatesȱandȱcleanȱwaterȱthrough.ȱȱ What you may need: Activity: 3 Extension Age: High school Time: 4 Why? Suspended solids is related to turbidity and indicates how many solid particles are in the water. These fine particles can clog fish gills and foul benthic filter feeders. Safety: Heat risks apply as TSS is dried in an oven. – siteȱrecordȱformȱȱ – oneȬlitreȱsamplingȱbottleȱwithȱlidȱ – 500ȬmLȱmeasuringȱcontainerȱȱ – aȱvacuumȱpumpȱforȱuseȱinȱtheȱfield,ȱorȱaccessȱtoȱaȱlaboratoryȱwithȱaȱsuitableȱpumpȱȱ – 500ȬmLȱvacuumȱflaskȱtoȱfitȱpumpȱ – 47Ȭmmȱglassȱfibreȱ(GDȬ120)ȱfilterȱpaperȱ – bottleȱofȱdistilledȱorȱdemineralisedȱwaterȱȱ – forcepsȱȱ – smallȱsquareȱtraysȱmadeȱofȱaluminiumȱfoil,ȱmarkedȱwithȱsampleȱnumbersȱ(oneȱperȱsample)ȱ – dryingȱovenȱ – heatproofȱorȱheatȱresistantȱglovesȱ – laboratoryȱscalesȱaccurateȱtoȱ0.0002ȱgȱ – aȱdesiccatorȱorȱaȱflat,ȱliddedȱcontainerȱcontainingȱsilicaȱdryingȱcrystals.ȱ To ensure good results: Doȱnotȱexceedȱ400–mmȱmercuryȱvacuumȱwhenȱpumping,ȱorȱtheȱfilterȱpaperȱmayȱbreakȱdownȱorȱ sedimentȱmayȱbeȱforcedȱthroughȱtheȱfilter.ȱ Itȱisȱeasierȱtoȱcentreȱtheȱfilterȱpaperȱonȱtheȱfilterȱholderȱifȱtheȱfilterȱisȱslightlyȱwet.ȱ Continuousȱstirringȱorȱshakingȱofȱtheȱsampleȱnotȱyetȱinȱtheȱfilteringȱapparatusȱisȱrecommended.ȱ Itȱisȱbestȱtoȱfilterȱasȱmuchȱwaterȱasȱpossibleȱ(upȱtoȱoneȱlitre).ȱItȱisȱalsoȱimportantȱnotȱtoȱclogȱtheȱfilter,ȱasȱ allȱwaterȱinȱtheȱfilteringȱapparatusȱmustȱbeȱfiltered.ȱTheȱbestȱwayȱtoȱmakeȱsureȱofȱthisȱisȱtoȱonlyȱputȱ smallȱamountsȱofȱwaterȱinȱtheȱfilteringȱcontainerȱatȱaȱtime.ȱȱ Ifȱtheȱfilterȱstartsȱtoȱbecomeȱclogged,ȱorȱtheȱflowȱofȱwaterȱthroughȱtheȱfilterȱslowsȱdown,ȱceaseȱfilteringȱasȱ soonȱasȱpossibleȱandȱrecordȱtheȱvolumeȱfilteredȱonȱyourȱdatasheet.ȱIfȱtheȱfilterȱbecomesȱcompletelyȱ cloggedȱbeforeȱallȱtheȱwaterȱinȱtheȱfilteringȱcontainerȱcanȱbeȱfilteredȱthenȱtheȱtestȱwillȱneedȱtoȱbeȱredone.ȱ Beforeȱdryingȱandȱweighing,ȱtheȱfilterȱpaperȱneedsȱtoȱbeȱthoroughlyȱrinsedȱwithȱdistilledȱwaterȱtoȱ removeȱanyȱdissolvedȱsaltsȱthatȱmayȱbeȱcaughtȱinȱtheȱfibres.ȱYouȱareȱonlyȱmeasuringȱsolids,ȱnotȱsolubleȱ salts.ȱ 67 ESTUARINE MONITORING GUIDANCE MANUAL What to do: CollectȱaȱoneȬlitreȱsampleȱofȱtheȱwaterȱbody,ȱfromȱ200Ȭmmȱbelowȱtheȱsurface.ȱ Usingȱforcepsȱplaceȱaȱfilterȱpaperȱontoȱanȱaluminiumȱtrayȱandȱweighȱthem.ȱRecordȱtheȱweightȱtoȱtheȱlastȱ decimalȱplaceȱonȱyourȱdatasheet.ȱȱ Pourȱ200–mLȱofȱtheȱfirstȱsampleȱintoȱtheȱmeasuringȱflask.ȱPlaceȱtheȱfilterȱpaperȱinȱtheȱfilter,ȱensuringȱthatȱ theȱwrinkledȱsideȱofȱtheȱpaperȱfacesȱupwards.ȱPourȱ100ȬmLȱfromȱtheȱmeasuringȱflaskȱintoȱtheȱtopȱofȱtheȱ filterȱdeviceȱandȱuseȱtheȱpumpȱtoȱcreateȱaȱvacuumȱinȱtheȱflask.ȱKeepȱtheȱfilterȱpaperȱwetȱorȱimmersedȱ usingȱwaterȱfromȱtheȱflask,ȱbutȱbeȱcarefulȱnotȱtoȱblockȱtheȱfilter.ȱȱ Onceȱtheȱflaskȱhasȱbeenȱemptiedȱrefillȱtoȱ200ȬmLȱlineȱandȱcontinueȱfiltering.ȱȱ Afterȱallȱwaterȱhasȱbeenȱfilteredȱorȱtheȱfilterȱhasȱstartedȱtoȱblockȱupȱandȱtheȱfilteringȱdeviceȱisȱempty,ȱ pourȱ100–200ȱmLȱofȱdistilledȱwaterȱintoȱtheȱfilterȱdeviceȱtoȱrinseȱanyȱsaltȱoutȱofȱtheȱfilter.ȱOnceȱtheȱrinseȱ waterȱhasȱbeenȱfiltered,ȱpourȱorȱsprayȱdistilledȱwaterȱontoȱtheȱwallsȱofȱtheȱfilteringȱdeviceȱtoȱensureȱthatȱ allȱsedimentȱhasȱbeenȱwashedȱontoȱtheȱfilter.ȱȱ Afterȱtheȱfilterȱpaperȱhasȱbeenȱsuckedȱdry,ȱrecordȱtheȱvolumeȱofȱsampleȱthatȱwasȱfiltered.ȱTheȱcolourȱofȱ theȱsedimentȱonȱtheȱfilterȱmayȱalsoȱbeȱrecorded.ȱ Usingȱtheȱforceps,ȱgentlyȱremoveȱtheȱfilterȱpaperȱfromȱtheȱfilterȱscreen,ȱensuringȱthatȱtheȱupperȱsurfaceȱ ofȱtheȱfilterȱpaperȱremainsȱuntouched.ȱPlaceȱtheȱfilterȱonȱitsȱlabelledȱaluminiumȱtray.ȱȱ Placeȱtheȱfilterȱandȱitsȱtrayȱintoȱtheȱdryingȱovenȱatȱ100°Cȱforȱoneȱhour.ȱ Wearingȱheatproofȱgloves,ȱremoveȱtheȱtrayȱandȱfilterȱfromȱtheȱoven.ȱAllowȱthemȱtoȱcoolȱinȱtheȱdesiccatorȱ untilȱtheyȱareȱatȱroomȱtemperatureȱthenȱweighȱthemȱcarefully.ȱȱ Recordȱtheȱweightȱonȱtheȱdataȱsheet.ȱȱ Calculateȱtheȱconcentrationȱofȱsuspendedȱsolidsȱperȱlitreȱusingȱtheȱformulaȱbelow:ȱ SS = B-A x (1000/C) x 1000 where: SS = Suspended solids in mg/L A = Unused (starting) filter paper and tray (g) B = Used filter (ending) paper and tray (g) C = Volume of sample filtered (mL) Theȱdataȱisȱpresentedȱasȱmg/Lȱsuspendedȱsolids.ȱIfȱmultipleȱsitesȱareȱvisited,ȱaȱmapȱofȱtheȱsuspendedȱ solidsȱconcentrationsȱinȱtheȱestuaryȱmayȱbeȱmadeȱusingȱaȱtopographicȱmapȱandȱsomeȱcolouredȱpencils.ȱ Ifȱyouȱregularlyȱtestȱforȱsuspendedȱsolidsȱoverȱaȱperiodȱofȱtimeȱatȱoneȱlocation,ȱyouȱmayȱbeȱableȱtoȱ correlateȱthemȱwithȱrainȱorȱotherȱeventsȱthatȱmayȱraiseȱtheȱsuspendedȱsolidȱconcentrationȱinȱyourȱ estuaryȱorȱatȱyourȱsite.ȱȱ Ifȱwaterȱclarityȱorȱturbidityȱareȱmeasuredȱatȱtheȱsameȱsiteȱandȱtimeȱasȱtheȱsuspendedȱsolids,ȱaȱ relationshipȱbetweenȱtheseȱmeasurementsȱcouldȱbeȱdeterminedȱforȱyourȱsite(s).ȱ 68 ESTUARINE MONITORING GUIDANCE MANUAL Mass balances to estimate pollutants TheȱNationalȱPollutantȱInventoryȱ(NPI)ȱisȱAustralia’sȱnationalȱ databaseȱofȱpollutantȱemissions.ȱInȱ2006,ȱtheȱonlineȱdatabaseȱheldȱ emissionȱdataȱforȱmoreȱthanȱ3700ȱfacilities,ȱinȱ29ȱcatchmentsȱaroundȱ Australia.ȱLargeȱindustriesȱprovideȱinformationȱtoȱtheȱgovernmentȱ onȱtheirȱemissionsȱtoȱair,ȱlandȱandȱwaterȱusingȱaȱtechniqueȱreferredȱ toȱasȱmassȱbalance.ȱLocalȱandȱstateȱgovernmentsȱprovideȱadditionalȱ dataȱtoȱtheȱNPIȱthatȱincludesȱtheȱmoreȱdiffuseȱpollutionȱsources,ȱ suchȱasȱstormwaterȱrunoff.ȱ IndividualsȱmayȱaccessȱtheȱNPIȱdatabaseȱonlineȱtoȱexamineȱareasȱofȱ interestȱtoȱthem.ȱByȱcallingȱupȱaȱmapȱofȱyourȱestuaryȱyouȱcanȱseeȱ thoseȱfacilitiesȱlargeȱenoughȱtoȱbeȱrequiredȱtoȱreportȱtoȱtheȱNPI,ȱandȱ obtainȱstatisticsȱonȱtheȱemissionȱloadsȱtheyȱdischargeȱtoȱland,ȱairȱorȱ water.ȱȱ Inȱthisȱwayȱyouȱmayȱestimateȱtheȱtotalȱloadsȱofȱpollutantsȱthatȱareȱ dischargedȱintoȱtheȱwaterway.ȱTheȱfirstȱactivityȱinȱthisȱsectionȱ showsȱyouȱhowȱtoȱaccessȱtheȱNPIȱdatabase.ȱ Theȱmassȱbalanceȱtechniqueȱisȱaȱsimpleȱmethodȱthatȱcanȱbeȱappliedȱ toȱallȱtypesȱofȱdevelopments,ȱnotȱjustȱlargeȱindustries.ȱTheȱmethodȱ mayȱbeȱappliedȱtoȱyourȱhome,ȱschoolȱorȱlocalȱsmallȱbusinesses.ȱReadȱ theȱsectionȱbelowȱthatȱdescribesȱhowȱtoȱperformȱaȱmassȱbalance.ȱOneȱ ofȱtheȱbenefitsȱofȱaȱmassȱbalanceȱisȱthatȱtheȱmethodȱhighlightsȱareasȱ whereȱwasteȱisȱbeingȱproduced.ȱȱ Activity: Extension Age: High School Time: 4 Why? Many contaminants that end up in estuaries are toxic to marine life. These activities describe how to calculate the overall load of contaminants entering into an estuary, and how to determine what contaminants may be coming from a single facility. Safety: This is a desk-based activity. Thinkȱaboutȱwasteȱforȱaȱmoment.ȱIsn’tȱaȱwasteȱsomethingȱyouȱhaveȱpaidȱmoneyȱforȱoriginally,ȱbutȱareȱ nowȱthrowingȱaway?ȱIfȱyouȱreduceȱwaste,ȱyouȱreduceȱyourȱupȬfrontȱcosts,ȱsavingȱyourselfȱmoneyȱasȱ wellȱasȱreducingȱimpactsȱonȱtheȱenvironment.ȱMaybeȱtheȱmassȱbalanceȱyouȱundertakeȱwillȱshowȱyouȱaȱ wayȱthatȱyourȱgroupȱcouldȱreduceȱwaste,ȱsaveȱmoneyȱandȱhelpȱtheȱenvironment.ȱ What you may need: TheȱNPIȱwebsiteȱatȱ<www.npi.gov.au>,ȱandȱvisitȱtheȱ‘Makeȱaȱmap’ȱlink.ȱȱ What to do: Lookȱatȱtheȱoverallȱpollutantȱloadsȱgoingȱintoȱyourȱestuary.ȱGoȱtoȱtheȱNPIȱwebsiteȱatȱ<www.npi.gov.au>ȱ andȱclickȱonȱtheȱbuttonȱthatȱwillȱopenȱtheȱ‘MakeȱaȱMap’ȱwindow.ȱAȱmapȱofȱAustraliaȱwithȱmanyȱblueȱ squaresȱmarkingȱindustriesȱthatȱareȱreportingȱtoȱtheȱNPIȱwillȱbeȱdisplayed.ȱAtȱtheȱtopȱofȱtheȱmapȱisȱaȱ rowȱofȱ‘tools’.ȱTheȱfirstȱtoolȱisȱaȱ‘+’ȱsymbol.ȱClickȱonȱthisȱsymbol,ȱthenȱuseȱyourȱmouseȱtoȱdrawȱaȱ‘box’ȱ aroundȱtheȱareaȱthatȱinterestsȱyou.ȱTheȱmapȱwillȱredraw,ȱandȱyouȱwillȱbeȱcloserȱtoȱoutliningȱtheȱareaȱofȱ yourȱestuary.ȱContinueȱenlargingȱtheȱmapȱuntilȱjustȱyourȱestuaryȱisȱcontainedȱinȱtheȱmapȱpage.ȱ Onȱtheȱtoolbarȱyouȱwillȱseeȱaȱselectionȱofȱgreenȱcirclesȱwithȱdottedȱoutlinesȱinȱthem.ȱTheseȱareȱselectionȱ tools.ȱTheyȱenableȱyouȱtoȱselectȱfacilitiesȱtoȱexamine.ȱChooseȱtheȱshapeȱ(circle,ȱrectangleȱorȱirregularȱ polygon)ȱthatȱwillȱallowȱyouȱtoȱcaptureȱallȱtheȱfacilitiesȱthatȱmayȱcontributeȱtoȱtheȱestuaryȱpollutionȱ loads,ȱandȱdrawȱyourȱselectionȱontoȱtheȱmap.ȱ Thisȱtimeȱtheȱprogramȱwillȱopenȱaȱwindowȱthatȱtellsȱyouȱhowȱmanyȱfacilitiesȱareȱinȱtheȱareaȱyouȱ selected.ȱIfȱyouȱclickȱyourȱmouseȱonȱtheȱwordȱ‘Facilities’ȱyouȱwillȱbeȱprovidedȱwithȱaȱlistȱofȱfacilitiesȱandȱ aȱlinkȱtoȱeachȱofȱtheirȱpollutantȱloadȱreports.ȱTheȱtablesȱshowȱwhetherȱaȱpollutantȱisȱreleasedȱtoȱland,ȱairȱ orȱwater.ȱTheȱfacilitiesȱdoȱnotȱreportȱanythingȱthatȱtheyȱsendȱtoȱsewerȱorȱlandfill,ȱasȱtheseȱpollutantsȱwillȱ beȱrecordedȱasȱtransfersȱbyȱthoseȱfacilities.ȱHenceȱanyȱpollutantȱrecordedȱasȱdischargingȱtoȱwater,ȱmayȱ beȱdischargingȱintoȱtheȱestuary.ȱ 69 ESTUARINE MONITORING GUIDANCE MANUAL Totalȱupȱallȱtheȱfacilities’ȱdischargesȱtoȱwater.ȱThisȱisȱtheȱminimumȱtotalȱloadȱofȱpollutantsȱthatȱyourȱ estuaryȱreceivesȱinȱanyȱgivenȱyear.ȱSo,ȱisȱthisȱloadȱaȱbigȱload,ȱorȱaȱlittleȱload?ȱThatȱisȱaȱdifficultȱquestion.ȱ TheȱNPIȱranksȱtheȱloads,ȱsoȱaȱfacilityȱwithȱaȱloadȱrankȱofȱ100ȱisȱtheȱlargestȱproducerȱofȱthatȱwasteȱinȱtheȱ country,ȱwhileȱaȱrankingȱofȱ1ȱshowsȱthatȱtheȱfacilityȱisȱinȱtheȱ1%ȱofȱsmallestȱproducersȱofȱthatȱwaste.ȱTheȱ rankingȱisȱrelative,ȱnotȱbasedȱonȱfixedȱquantities.ȱȱ Additionally,ȱaȱlargeȱestuary,ȱwithȱlargeȱtidesȱandȱaȱpositiveȱriverȱflowȱmayȱbeȱableȱtoȱacceptȱmuchȱ largerȱloadsȱthanȱaȱsmall,ȱmicrotidalȱestuaryȱwithȱlittleȱfreshwaterȱinput,ȱbeforeȱenvironmentalȱimpactsȱ becomeȱobvious.ȱ Conduct ing ȱ aȱ s im p l eȱmassȱ ba lan ce :ȱ ȱ Chooseȱaȱsmallȱfacilityȱtoȱdevelopȱaȱmassȱbalanceȱfor.ȱItȱmayȱbeȱyourȱhome,ȱschoolȱorȱaȱsmallȱlocalȱ business.ȱIfȱtheȱlatter,ȱrememberȱthatȱanyȱinformationȱaȱlocalȱbusinessȱsharesȱwithȱyouȱisȱconfidential.ȱ Localȱbusinessesȱareȱoftenȱinterestedȱinȱimprovingȱtheirȱenvironmentalȱperformance,ȱandȱaȱlackȱofȱ discretionȱonȱtheȱgroup’sȱpartȱmayȱbeȱcounterproductive.ȱȱ Aȱmassȱbalanceȱassumesȱthatȱeverythingȱthatȱentersȱtheȱfrontȱgateȱofȱaȱfacilityȱ(rawȱmaterials,ȱenergy,ȱ fuel)ȱgoesȱsomewhere.ȱTheȱmaterialsȱeitherȱbecomeȱpartȱofȱtheȱproduct,ȱareȱconvertedȱtoȱsomethingȱelseȱ (heat,ȱsmoke,ȱsteam)ȱorȱareȱexpelledȱfromȱtheȱsiteȱbyȱsomeȱotherȱmeansȱ(downȱsewersȱandȱdrains,ȱorȱinȱ theȱgarbageȱcan).ȱ Inȱaȱhugeȱfactory,ȱtrackingȱallȱtheȱthingsȱthatȱenterȱtheȱsiteȱmayȱbeȱextremelyȱcomplex.ȱHowever,ȱ businessesȱhaveȱoneȱadvantage—theirȱaccountsȱrevealȱwhatȱtheyȱboughtȱin,ȱandȱtheȱfeesȱpertainingȱtoȱatȱ leastȱsomeȱofȱtheirȱdisposalȱcosts,ȱalongȱwithȱtheȱquantitiesȱofȱproductȱsold.ȱTypicallyȱauditorsȱdoingȱaȱ massȱbalanceȱwillȱaskȱquestionsȱsuchȱasȱ‘Whyȱdoȱ100ȱtonnesȱofȱwoodchipȱonlyȱproduceȱ80ȱtonnesȱofȱ fibreboard?’ȱtoȱfindȱoutȱwhereȱwasteȱisȱformed.ȱ Inȱaȱsmallȱoperation,ȱitȱisȱeasierȱtoȱseeȱwhatȱhappensȱtoȱmaterials,ȱbutȱhouseholdȱmassȱbalancesȱareȱ complicatedȱbyȱtheȱfactȱthatȱfewȱhouseholdsȱkeepȱaccounts,ȱsoȱyouȱneedȱtoȱtakeȱestimatesȱforȱthingsȱlikeȱ howȱmuchȱfood,ȱdetergentȱorȱpetrolȱisȱpurchasedȱeachȱyear.ȱ Theȱdiagramȱ(Figureȱ9)ȱshowsȱaȱmaterialȱflowȱinȱaȱfishȱandȱchipȱoperation.ȱByȱputtingȱfiguresȱonȱtheȱ inputsȱandȱoutputs,ȱyouȱwouldȱbeȱableȱtoȱdetermineȱtheȱamountȱofȱeachȱwasteȱandȱitsȱdestination.ȱ ȱ Figure 9—Material flow in a fish and chip shop 70 ESTUARINE MONITORING GUIDANCE MANUAL Measuring soil salinity and soil pH SoilȱsalinityȱandȱpHȱareȱtwoȱimportantȱindicatorsȱofȱsaltmarshȱ ecologicalȱhealth,ȱparticularlyȱinȱareasȱthatȱhaveȱpotentialȱacidȱ sulphateȱsoilsȱorȱsalinityȱproblems.ȱSalinityȱreadingsȱareȱalsoȱusefulȱ forȱstudentsȱdoingȱhydrologicalȱstudiesȱonȱsaltmarshesȱandȱ supratidalȱorȱintertidalȱestuarineȱecosystems.ȱChangesȱtoȱtheȱ hydrologicalȱregimeȱmayȱgreatlyȱinfluenceȱbothȱsoilȱsalinityȱandȱpH.ȱȱ Soilȱsalinityȱisȱstronglyȱinfluencedȱbyȱtidalȱflushing,ȱevaporationȱandȱ rainfall.ȱTheȱsupratidalȱareasȱareȱtheȱmostȱsalineȱdueȱtoȱinfrequentȱ tidalȱflushingȱcombinedȱwithȱevaporation.ȱAreasȱthatȱreceiveȱgreaterȱ tidalȱflushingȱareȱlessȱsaline.ȱȱ SoilȱsalinityȱandȱsoilȱpHȱareȱmeasuredȱusingȱsimilarȱmethods,ȱandȱ bothȱmayȱbeȱdoneȱtogetherȱinȱoneȱsampleȱorȱindividually.ȱTheseȱ factorsȱareȱnotȱoftenȱmeasuredȱinȱisolation,ȱbutȱasȱpartȱofȱanȱoverallȱ collectionȱofȱinformationȱwhenȱdoingȱvegetationȱassessmentsȱorȱ hydrologicalȱstudies.ȱȱ Theȱmethodȱgivenȱbelowȱusesȱaȱ1:5ȱsoil–waterȱsolutionȱwithȱfieldȱ equipment.ȱMoreȱaccurateȱresultsȱcanȱbeȱobtainedȱusingȱlaboratoryȱ equipment,ȱalthoughȱtheȱequipmentȱspecifiedȱwillȱgiveȱaȱreasonableȱ result.ȱTheȱ1:5ȱsolutionȱmethodȱwasȱselectedȱforȱitsȱwidespreadȱuseȱ andȱeaseȱofȱmeasurement.ȱ What you may need: – saltmarshȱrecordȱformȱ – topographicȱmapȱ(orȱGPS)ȱ – smallȱhandȱtrowelȱȱ – smallȱflatȱtraysȱorȱboxesȱforȱdryingȱsoilȱȱ – glassȱscrew–toppedȱcontainerȱmarkedȱatȱ300ȱmLȱȱ – distilledȱwaterȱȱ – hydrometer(s),ȱthermometerȱandȱpHȱstripsȱ – pocketȱscalesȱaccurateȱtoȱ0.2ȱgȱwithȱaȱmaximumȱmassȱofȱ100ȱgȱ – batteryȱoperatedȱtimerȱ – mechanicalȱstirringȱdeviceȱ(optional)ȱ – tubeȱforȱhydrometerȱreadings.ȱ Activity: 3 Extension Age: High school Time: 2 or 3 Why? The salinity and pH of saltmarsh soils determines the types of vegetation that will develop. These parameters may change rapidly after tidal gates or impoundments are placed in intertidal areas, resulting in large changes to the vegetation. In some cases there may be releases of acid from the oxidized soil into the main estuary. Safety: Glass equipment needs to be handled gently. Use a sharps container for broken glass. Dispose of all reagent/sample mixes appropriately. Saltmarshes may contain rough terrain and ill-defined waterways. To ensure good results: Checkȱyourȱequipmentȱbeforeȱgoingȱoutȱintoȱtheȱfield.ȱBackȱatȱbase,ȱcheckȱyourȱreagentsȱandȱequipmentȱ beforeȱtestingȱtheȱsoilȱsamples.ȱSeeȱMeasuringȱpHȱandȱMeasuringȱsalinityȱforȱmoreȱdetails.ȱ Cleanȱyourȱsamplingȱequipmentȱ(trowel)ȱbetweenȱsamplesȱtoȱpreventȱcontaminationȱofȱtheȱnextȱsample.ȱ What to do: Decideȱwhereȱyouȱwillȱtestȱtheȱsoil—youȱmayȱobtainȱmoreȱusefulȱinformationȱifȱyouȱmakeȱaȱtraverseȱ awayȱfromȱtheȱwaterwayȱtowardsȱhighȱland,ȱsamplingȱatȱintervals.ȱRecordȱyourȱsamplingȱlocationsȱonȱaȱ topographicȱmapȱorȱbyȱusingȱaȱGPS.ȱ Atȱeachȱsamplingȱlocation,ȱuseȱtheȱhandȱtrowelȱtoȱcollectȱaȱsmallȱsoilȱsampleȱfromȱaboutȱ100ȱmmȱbelowȱ theȱsurface.ȱAroundȱaȱhandfulȱwillȱdo.ȱPlaceȱitȱinȱaȱlabelledȱplasticȱbag.ȱRepeatȱthisȱforȱasȱmanyȱsamplesȱ asȱyouȱareȱtesting.ȱOnceȱallȱsamplesȱareȱcollected,ȱreturnȱtoȱyourȱbaseȱandȱplaceȱeachȱsampleȱinȱaȱ 71 ESTUARINE MONITORING GUIDANCE MANUAL separateȱdryingȱtrayȱinȱaȱwarmȱspotȱ(butȱ<40°C)ȱuntilȱallȱtheȱsamplesȱareȱdryȱandȱcrumbly.ȱThisȱusuallyȱ takesȱatȱleastȱ24ȱhours.ȱIfȱtheȱsoilȱisȱdryȱtoȱtouchȱpriorȱtoȱairȬdrying,ȱtestingȱcanȱcommenceȱimmediately,ȱ inȱtheȱfield,ȱbutȱthisȱisȱunusualȱinȱsaltmarshȱareas.ȱȱ Fillȱtheȱliddedȱglassȱcontainerȱtoȱtheȱ300ȬmLȱmarkȱwithȱdistilledȱwater.ȱWeighȱ60gȱofȱsoilȱandȱplaceȱinȱ theȱdistilledȱwaterȱandȱscrewȱtheȱlidȱonȱwell.ȱȱ Eitherȱshakeȱvigorouslyȱforȱtwoȱminutesȱeveryȱ15ȱminutesȱforȱtwoȱhoursȱ(aȱtotalȱofȱ16ȱminutesȱofȱ shaking)ȱorȱplaceȱonȱtheȱmechanicalȱstirrerȱforȱoneȱhour.ȱȱ Leaveȱsampleȱtoȱsettleȱforȱ20ȱminutesȱandȱtakeȱaȱpHȱreadingȱaccordingȱtheȱMeasuringȱpHȱguideline.ȱ RecordȱtheȱpHȱvalueȱonȱyourȱrecordȱsheet.ȱ Decantȱtheȱclearȱ(orȱsemiȬclear)ȱliquidȱintoȱtheȱtubeȱusedȱforȱhydrometerȱreadings.ȱAtȱleastȱ200ȱmLȱisȱ needed.ȱUseȱtheȱhydrometer(s)ȱtoȱtakeȱaȱspecificȱgravityȱreadingȱaccordingȱtheȱMeasuringȱsalinityȱȱ guideline.ȱRecordȱtheȱtemperatureȱofȱtheȱliquidȱtoȱenableȱyouȱtoȱmakeȱaȱtemperatureȱcorrectionȱlater.ȱ Theȱ1:5ȱsoil–waterȱmethodȱofȱanalysisȱdoesȱnotȱprovideȱtheȱtrueȱsoilȱsalinityȱorȱpHȱreadingȱthatȱaȱplantȱ wouldȱencounterȱinȱtheȱactualȱenvironment.ȱHowever,ȱtheȱmethodȱdoesȱprovideȱaȱwellȱrecognisedȱ indicationȱofȱsoilȱconditions.ȱThereȱareȱseveralȱsoilȱsalinityȱandȱpHȱmeasurementȱmethods.ȱEachȱmethodȱ hasȱitsȱmeritsȱandȱisȱusedȱforȱdifferentȱreasons.ȱTheȱ1:5ȱsoil–waterȱsolutionȱmethodȱisȱtheȱmostȱ commonlyȱusedȱinȱAustralia,ȱandȱthereȱisȱaȱlargeȱdatabaseȱofȱplantȱsalinityȱtolerancesȱforȱthisȱmethod.ȱ EnsureȱthatȱanyȱdataȱyouȱrecordȱorȱuseȱaboutȱsoilȱsalinityȱorȱpHȱstatesȱtheȱmethodȱused.ȱTheȱstandardȱ protocolȱisȱtoȱreportȱtheȱmethodȱasȱaȱsubscript.ȱSoilȱpHȱmayȱbeȱreportedȱas:ȱpH(1:5)ȱ=ȱ6.9,ȱforȱexample.ȱ Potential,ȱandȱactual,ȱacidȱsulphateȱsoilsȱareȱfoundȱinȱmanyȱcoastalȱdepositionalȱenvironmentsȱsuchȱasȱ areasȱofȱmangroveȱandȱsaltmarsh.ȱManagingȱtheseȱareasȱsoȱasȱnotȱtoȱincreaseȱtheȱchanceȱofȱoxidisingȱtheȱ soilsȱandȱcausingȱacidȱgenerationȱisȱimportant.ȱInȱ2003ȱtheȱSouthȱAustralianȱCoastȱProtectionȱBoardȱ producedȱanȱissueȱofȱtheirȱnewsletterȱCoastlineȱthatȱdiscussesȱcoastalȱacidȱsulphateȱsoils.ȱCoastlineȱ33ȱmayȱ beȱobtainedȱfromȱtheȱCoastȱandȱMarineȱBranchȱofȱtheȱDepartmentȱforȱEnvironmentȱandȱHeritage,ȱorȱ downloadedȱfromȱtheirȱwebsiteȱ<www.environment.sa.gov.au/coasts/pub.html>.ȱ ȱ ȱ ȱ 72 ȱE S T U A R I N E MONITORING GUIDANCE MANUAL Looking at the vegetation and habitat ȱ ȱ ȱ ȱ 73 ESTUARINE MONITORING GUIDANCE MANUAL Keeping a visual record of plankton blooms Inȱtheȱestuarineȱenvironment,ȱjustȱasȱinȱourȱownȱbodies,ȱthereȱisȱsuchȱaȱ thingȱasȱtooȱmuchȱofȱaȱgoodȱthing.ȱEvenȱthoughȱnutrientsȱareȱessentialȱ forȱgrowthȱandȱsurvival,ȱtooȱmuchȱofȱthemȱwillȱresultȱinȱbloomsȱofȱ planktonicȱalgae.ȱ Activity: 3 Core Age: All Theseȱbloomsȱmayȱaffectȱtheȱestuaryȱinȱseveralȱways.ȱWaterȱcloudedȱ byȱplanktonȱdoesȱnotȱallowȱsunlightȱtoȱpenetrateȱtoȱtheȱbenthicȱzone.ȱ Seagrassesȱandȱotherȱsubmergedȱplantsȱthatȱrequireȱlightȱmayȱdieȱifȱ theȱwaterȱisȱpermanentlyȱturbid.ȱ Time: 1 Whileȱinȱtheȱdaylight,ȱplanktonȱproduceȱoxygenȱbyȱphotosynthesis,ȱinȱ theȱdarkȱtheyȱuseȱitȱtoȱrespire.ȱWhenȱthereȱisȱaȱlargeȱbloomȱthereȱisȱaȱ hugeȱswingȱeachȱday/nightȱcycleȱinȱtheȱamountȱofȱdissolvedȱoxygenȱ (DO)ȱinȱtheȱwater.ȱIfȱtheȱDOȱfallsȱfarȱenough,ȱtheȱwaterȱbecomesȱ hypoxicȱ(lowȱinȱoxygen),ȱputtingȱmassiveȱstressȱonȱallȱlifeȱinȱtheȱwater.ȱ Onceȱtheȱwaterȱofȱanȱestuaryȱhasȱlessȱthanȱ4ȱmg/LȱDO,ȱfishȱmayȱstartȱ toȱdie.ȱ Safety: Algal blooms may be toxic. Do not get into water that has a dense bloom and use gloves while collecting samples. Why? Plankton blooms are often the result of high nutrient concentrations. Planktonȱbloomsȱareȱoftenȱmadeȱupȱofȱcertainȱgroupsȱofȱplanktonicȱalgaeȱthatȱbelongȱtoȱtheȱ dinoflagellateȱgroup.ȱThisȱisȱbecauseȱtheȱwasteȱstreamsȱthatȱprovideȱsoȱmuchȱnitrogenȱandȱphosphorusȱ areȱoftenȱshortȱofȱotherȱelementsȱsuchȱasȱsilica,ȱwhichȱisȱrequiredȱbyȱmanyȱvaluableȱplanktonȱsuchȱasȱ diatoms.ȱThisȱleadsȱtoȱaȱsituationȱwhereȱonlyȱcertainȱtypesȱofȱphytoplanktonȱsuchȱasȱtheȱdinoflagellatesȱ canȱgrow.ȱTheseȱbloomsȱareȱoftenȱcalledȱredȱtidesȱandȱbrownȱtidesȱandȱsomeȱofȱtheseȱalgaeȱproduceȱ toxinsȱthatȱmayȱkillȱfishȱorȱaccumulateȱinȱshellfish,ȱpoisoningȱpeople.ȱ InȱSouthȱAustralia,ȱtheȱPortȱRiverȱsupportsȱregularȱbloomsȱofȱtheȱtoxicȱdinoflagellateȱAlexandriumȱ minutum.ȱ What you may need: – siteȱrecordȱformȱ – Mitrovic,ȱSȱ(1995),ȱWhatȱScumȱisȱThat?,ȱWaterȱQualityȱServicesȱUnit,ȱDepartmentȱofȱLandȱ&ȱWaterȱ Conservation,ȱSydney.ȱ What to do: Onȱarrivalȱatȱyourȱsite,ȱlookȱforȱanyȱred,ȱbrownȱorȱyellowȱcolourȱinȱtheȱwater,ȱorȱanyȱscumȱfloatingȱonȱ theȱsurface.ȱIfȱthereȱisȱaȱslightȱcolourationȱonly,ȱrecordȱtheȱcolourȱonȱyourȱsiteȱrecordȱform.ȱ Ifȱthereȱisȱanȱobviousȱbloomȱorȱscum,ȱattemptȱtoȱdescribeȱitȱorȱphotographȱit.ȱUsingȱaȱreferenceȱsuchȱasȱ WhatȱScumȱisȱThat?ȱtryȱtoȱdetermineȱwhatȱtypeȱofȱeventȱmayȱbeȱoccurring.ȱCollectȱasȱmuchȱdetailȱasȱyouȱ canȱfromȱtheȱsite—whatȱkindȱofȱareaȱcanȱyouȱseeȱaffected,ȱisȱtheȱbloomȱdeepȱinȱtheȱwaterȱcolumnȱorȱisȱitȱ floatingȱonȱtheȱsurface?ȱȱ YouȱmayȱwishȱtoȱcollectȱaȱsampleȱtoȱprovideȱtoȱyourȱWaterwatchȱcoordinator,ȱwhoȱmayȱbeȱableȱtoȱ identifyȱtheȱalgaeȱresponsible.ȱIfȱyouȱdoȱattemptȱtoȱcollectȱaȱsample,ȱrememberȱthatȱalgalȱbloomsȱmayȱbeȱ toxic—wearȱdisposableȱglovesȱwhileȱcollectingȱyourȱsample,ȱrinseȱoffȱtheȱoutsideȱofȱtheȱsampleȱbottleȱ withȱcleanȱwaterȱandȱensureȱyouȱwashȱyourȱhandsȱthoroughlyȱafterȱtheȱactivity.ȱ 74 ESTUARINE MONITORING GUIDANCE MANUAL Getting to know phytoplankton with a net tow PhytoplanktonȱareȱsingleȬcelledȱplantsȱthatȱoccurȱinȱmostȱnaturalȱ waterȱbodies.ȱTheyȱfloatȱfreelyȱasȱindividuals,ȱcoloniesȱorȱinȱropeȬlikeȱ strands.ȱTheyȱdoȱnotȱattachȱthemselvesȱtoȱsurfaces.ȱAȱmicroscopeȱisȱ neededȱtoȱseeȱthem,ȱasȱtheyȱareȱoftenȱonlyȱaȱfewȱ10sȱofȱmicronsȱ (thousandthsȱofȱmillimetre)ȱlong.ȱȱ Aȱsingleȱdropȱofȱestuarineȱwaterȱmayȱhaveȱhundredsȱofȱtheseȱ microscopicȱplantsȱinȱit.ȱTheseȱplantsȱareȱtheȱfoundationȱstoneȱforȱ ecologicalȱhealth.ȱPhytoplanktonȱdirectlyȱandȱindirectlyȱsupportȱ muchȱofȱourȱmarineȱlife,ȱincludingȱlargeȱanimalsȱlikeȱwhalesȱandȱ sharks.ȱUnfortunatelyȱsomeȱspeciesȱproduceȱtoxinsȱthatȱmayȱkillȱ humansȱorȱanyȱotherȱanimalȱthatȱconsumesȱenoughȱofȱthem.ȱRedȱ tidesȱareȱcausedȱbyȱaȱselectionȱofȱdinoflagellateȱspecies,ȱsomeȱofȱ whichȱproduceȱtoxinsȱandȱsomeȱthatȱareȱharmless.ȱȱ Activity: 3 Extension Age: High school Time: 3 ThisȱactivityȱintroducesȱtheȱuseȱofȱaȱveryȱfineȱplanktonȱtowȬnetȱandȱ someȱbasicȱidentificationȱmethodsȱtoȱhelpȱyouȱcollectȱandȱbecomeȱ familiarȱwithȱsomeȱofȱtheȱphytoplanktonȱfoundȱinȱyourȱestuary.ȱ Why? Different types of plankton are always present in the water. The dominant types vary depending on the conditions. For example, diatoms require a source of silica (usually present in riverine water entering the estuary) as well as nutrients, while dinoflagellates only require nutrients. Plankton ‘blooms’ are often the result of high nutrient concentrations in shallow estuarine waters. Ifȱyouȱwouldȱlikeȱtoȱcompleteȱaȱmoreȱdetailedȱassessmentȱofȱyourȱ estuarineȱphytoplankton,ȱfeelȱfreeȱtoȱcompleteȱtheȱactivitiesȱgivenȱinȱ theȱguidelineȱCountingȱplanktonȱinȱaȱwaterȱsample.ȱ Safety: Net tows may be conducted in deep or fastflowing waters. Overabundanceȱofȱphytoplanktonȱmayȱcauseȱbigȱswingsȱinȱdissolvedȱ oxygenȱconcentrationsȱinȱtheȱwater,ȱleadingȱtoȱaȱconditionȱcalledȱ hypoxia,ȱwhereȱtheȱlowȱoxygenȱconcentrationsȱmayȱcauseȱfishȱkills.ȱ What you may need: – 50–100ȱmicronȱphytoplanktonȱtowȱnetȱȱ – plasticȱcollectionȱbottleȱwithȱlidȱ – 100–200×ȱmicroscopeȱ(preferablyȱwithȱphaseȱcontrast)ȱ – microscopeȱslidesȱandȱcoverȱslipsȱ – dropperȱorȱpipetteȱ – alcohol,ȱmethylatedȱspritsȱorȱglycerineȱ(optional)ȱ – drawingȱbookȱforȱrecordingȱyourȱphytoplankton,ȱorȱdigitalȱcamera.ȱ What to do: Obtainingȱaȱphytoplanktonȱsampleȱfromȱyourȱestuaryȱisȱrelativelyȱeasy.ȱAllȱitȱrequiresȱisȱforȱyouȱtoȱ attachȱyourȱcollectionȱbottleȱ(withoutȱitsȱlid)ȱtoȱtheȱbaseȱofȱtheȱplanktonȱnet,ȱusingȱaȱscrewȱmountȱorȱaȱ wormȬdriveȱhoseȱclampȱtoȱensureȱtheȱbottleȱstaysȱinȱplace.ȱȱ Theȱnetȱisȱthenȱloweredȱoffȱtheȱendȱofȱaȱjettyȱorȱoverȱtheȱsideȱofȱaȱbridgeȱdirectlyȱintoȱtheȱmainȱflowȱofȱ theȱestuary.ȱThisȱisȱbestȱdoneȱjustȱafterȱhighȱtideȱwhenȱtheȱoutgoingȱflowȱisȱrelativelyȱstrong.ȱTieȱtheȱnetȱ ropeȱontoȱtheȱstructureȱandȱleaveȱitȱthereȱforȱapproximatelyȱ15ȱminutes.ȱAlternatively,ȱyouȱmayȱtowȱtheȱ netȱbehindȱaȱseaworthyȱboatȱatȱaȱslowȱspeed.ȱ Pullȱtheȱnetȱverticallyȱoutȱofȱtheȱwaterȱsoȱthatȱanyȱphytoplanktonȱonȱtheȱsidesȱofȱtheȱnetȱwashȱdownȱintoȱ theȱbottle.ȱOnceȱtheȱnetȱisȱonshore,ȱwashȱanythingȱleftȱonȱtheȱsidesȱofȱtheȱnetȱintoȱtheȱcollectionȱbottleȱ usingȱseawater.ȱCarefullyȱremoveȱtheȱcollectionȱbottleȱwithoutȱspillingȱit,ȱremoveȱanyȱlargeȱvisibleȱ objectsȱandȱscrewȱtheȱlidȱon.ȱThisȱbottleȱwillȱneedȱtakingȱbackȱtoȱtheȱlaboratoryȱimmediatelyȱtoȱexamineȱ underȱtheȱmicroscope.ȱȱ 75 ESTUARINE MONITORING GUIDANCE MANUAL Backȱinȱtheȱlaboratory,ȱtakeȱaȱsmallȱsampleȱofȱ theȱphytoplanktonȱandȱplaceȱaȱfewȱdropsȱontoȱaȱ microscopeȱslideȱusingȱaȱpipetteȱorȱdropper.ȱ Placeȱtheȱcoverȱslipȱonȱtopȱofȱtheȱwater.ȱȱ Ifȱyouȱhaveȱneverȱusedȱaȱmicroscopeȱhaveȱaȱ lookȱatȱaȱtypicalȱoneȱ(Figureȱ10).ȱOnceȱyouȱhaveȱ familiarisedȱyourselfȱwithȱyourȱmicroscope,ȱ placeȱyourȱslideȱandȱcoverȱslipȱonȱtheȱstage.ȱȱ Rotateȱnosepieceȱsoȱthatȱtheȱ10×ȱobjectiveȱisȱinȱ use,ȱthisȱgivesȱyouȱ100×ȱmagnification.ȱStartȱ withȱtheȱobjectiveȱdownȱnearȱtheȱslideȱandȱ coarselyȱfocusȱupwardsȱbyȱturningȱtheȱfocusȱ controlȱtowardsȱtheȱoperator.ȱBeȱcarefulȱifȱtryingȱ toȱfocusȱdownwardsȱasȱtheȱobjectiveȱcouldȱ smashȱintoȱtheȱslide.ȱȱ Useȱtheȱfineȱfocusȱcontrolȱtoȱgainȱtheȱclearestȱ image.ȱAdjustȱtheȱeyepiecesȱtoȱsuitȱyourȱeyes.ȱ Theȱeyepiecesȱadjustȱbothȱforȱdistanceȱbetweenȱ pupilsȱandȱindividualȱfocus.ȱȱ Itȱisȱoftenȱeasierȱtoȱlookȱthroughȱaȱbinocularȱ microscopeȱifȱyouȱdonȇtȱactuallyȱtouchȱtheȱ eyepiecesȱwithȱyourȱface,ȱbutȱviewȱdownȱtheȱ eyepiecesȱfromȱaȱslightȱdistance.ȱȱȱ ȱ Figure 10—A typical microscope Closeȱtheȱcondenserȱirisȱjustȱenoughȱtoȱgiveȱtheȱspecimenȱsomeȱcontrast.ȱItȱshouldȱbeȱaboutȱoneȱthirdȱ closed.ȱTooȱmuchȱclosureȱresultsȱinȱaȱdecreasedȱdepthȱofȱfield.ȱ Whatȱdoȱyouȱnowȱseeȱdownȱyourȱmicroscope?ȱDidȱyouȱnoticeȱthatȱsomeȱplantsȱswim?ȱIfȱtheȱplanktonȱ areȱmovingȱtooȱfastȱforȱyouȱtoȱfocusȱonȱthem,ȱtakeȱtheȱslideȱoutȱandȱplaceȱaȱdropȱofȱglycerineȱontoȱtheȱ slide.ȱThisȱwillȱmakeȱtheȱwaterȱthickerȱandȱharderȱforȱtheȱphytoplanktonȱtoȱswimȱthrough.ȱȱ Alcoholȱorȱmethylatedȱspiritsȱmayȱbeȱaddedȱtoȱtheȱwholeȱsampleȱifȱyouȱfindȱitȱeasierȱtoȱlookȱatȱdeadȱ specimens,ȱalthoughȱyouȱloseȱtheȱdifferentȱmovementȱmethods,ȱwhichȱmayȱhelpȱyouȱidentifyȱsomeȱ speciesȱorȱgroupsȱofȱplankton.ȱȱ Youȱcouldȱnowȱtryȱandȱdrawȱtheȱplanktonȱyouȱseeȱunderȱtheȱscope,ȱorȱtryȱandȱmatchȱthemȱupȱtoȱtheȱ picturesȱshownȱinȱFigureȱ11ȱofȱsomeȱphytoplanktonȱfoundȱinȱestuarineȱwetlandsȱnearȱAdelaide.ȱ Aȱdigitalȱcameraȱplacedȱonȱtheȱeyepieceȱmayȱbeȱableȱtoȱtakeȱphotographsȱofȱtheȱfieldȱofȱview,ȱandȱtheseȱ photographsȱmayȱbeȱdownloadedȱontoȱyourȱcomputer.ȱTheseȱcouldȱbeȱidentifiedȱbyȱaȱspecialistȱorȱbyȱ usingȱspecialistȱidentificationȱmaterials.ȱȱ Youȱmayȱwishȱtoȱprepareȱaȱlistȱofȱtheȱtypesȱofȱphytoplanktonȱpresentȱinȱyourȱsample,ȱalongȱwithȱanyȱofȱ theȱphotographsȱyouȱtookȱorȱtheȱdrawingsȱthatȱyouȱdid.ȱDisplayingȱthisȱisȱaȱpublicȱplaceȱhelpsȱraiseȱ publicȱawarenessȱofȱtheȱrangeȱofȱorganismsȱthatȱliveȱinȱyourȱestuarineȱwaterways.ȱȱ 76 ESTUARINE MONITORING GUIDANCE MANUAL ȱ ȱ Key to some plankton of South Australian estuaries ȱ ȱ Figure 11—Examples of phytoplankton 77 ESTUARINE MONITORING GUIDANCE MANUAL Counting plankton in a water sample PhytoplanktonȱareȱsingleȬcelledȱplantsȱthatȱoccurȱinȱmostȱnaturalȱ waterȱbodies.ȱTheyȱfloatȱfreelyȱasȱindividuals,ȱcoloniesȱorȱinȱropeȬlikeȱ strands.ȱTheyȱdoȱnotȱattachȱthemselvesȱtoȱsurfaces.ȱAȱmicroscopeȱisȱ neededȱtoȱseeȱthem,ȱasȱtheyȱareȱoftenȱonlyȱaȱfewȱtensȱofȱmicronsȱ (thousandthsȱofȱmillimetre)ȱlong.ȱȱ Aȱsingleȱdropȱofȱestuarineȱwaterȱmayȱhaveȱhundredsȱofȱtheseȱ microscopicȱplantsȱinȱit.ȱTheseȱplantsȱareȱtheȱfoundationȱstoneȱforȱ ecologicalȱhealth.ȱPhytoplanktonȱdirectlyȱandȱindirectlyȱsupportȱ muchȱofȱourȱmarineȱlife,ȱincludingȱlargeȱanimalsȱlikeȱwhalesȱandȱ sharks.ȱUnfortunatelyȱsomeȱspeciesȱproduceȱtoxinsȱthatȱmayȱkillȱ humansȱorȱanyȱotherȱanimalȱthatȱconsumesȱenoughȱofȱthem.ȱRedȱ tidesȱareȱcausedȱbyȱaȱselectionȱofȱdinoflagellateȱspecies,ȱsomeȱofȱ whichȱproduceȱtoxinsȱandȱsomeȱthatȱareȱharmless.ȱ Activity: 3 Extension Age: Adult groups Time: 4 Why? The number of plankton cells present in a water sample, as well as their type, provides information on the health of the estuary. Safety: Sample/reagent mixes should be disposed of appropriately. Thisȱactivityȱusesȱaȱcondensedȱwaterȱsampleȱandȱaȱbasicȱflowchartȱtoȱ helpȱyouȱidentifyȱandȱcountȱtheȱcommonȱphytoplanktonȱphyla.ȱ Theseȱareȱveryȱcoarseȱclassifications,ȱbutȱtheȱproportionsȱofȱoneȱphylumȱtoȱanotherȱmayȱprovideȱanȱ insightȱintoȱtheȱwaterȱqualityȱofȱtheȱestuary,ȱandȱtheȱriskȱofȱtoxicȱdinoflagellateȱorȱblueȬgreenȱblooms.ȱ Ifȱyouȱwouldȱlikeȱtoȱcompleteȱaȱmoreȱdetailedȱassessmentȱofȱyourȱestuarineȱphytoplankton,ȱfeelȱfreeȱtoȱ lookȱatȱsomeȱofȱtheȱotherȱidentificationȱaidsȱreferencedȱinȱthisȱguideline,ȱorȱtryȱtoȱobtainȱotherȱ informationȱfromȱyourȱlocalȱuniversityȱlibrary.ȱȱ What you may need: – 200–500ȱmLȱcollectionȱbottleȱwithȱlidȱ – 100–200×ȱmicroscopeȱ(preferablyȱwithȱphaseȱcontrast)ȱ – 250ȬmLȱmeasuringȱcylinderȱ – haemocytometerȱandȱcoverȱslipsȱ – dropperȱorȱpipetteȱ – 0.5ȱmLȱofȱLugol’sȱmediaȱperȱsampleȱ – aȱfineȱpieceȱofȱclearȱflexibleȱplasticȱpipingȱtoȱuseȱasȱaȱsiphonȱ – liddedȱbucketȱcontainingȱkittyȱlitterȱ – aȱplanktonȱcountȱdatasheet.ȱ To ensure good results: Identifyingȱphytoplanktonȱcanȱbeȱdifficult,ȱinȱfactȱdistinguishingȱphytoplanktonȱ(microscopicȱfloatingȱ plants)ȱfromȱzooplanktonȱ(microscopicȱswimmingȱanimals)ȱmayȱbeȱveryȱdifficult,ȱasȱthereȱareȱmanyȱ singleȬcelledȱclearȱanimalsȱthatȱfeedȱonȱphytoplankton,ȱandȱtheirȱfoodȱitemsȱareȱvisibleȱthroughȱtheirȱcellȱ walls.ȱȱ Withoutȱaȱgreatȱdealȱofȱexperienceȱyouȱcan’tȱbeȱentirelyȱsureȱofȱwhatȱyouȱareȱlookingȱatȱallȱtheȱtime.ȱ Evenȱidentificationȱguidesȱareȱnotȱfoolproof,ȱasȱonlyȱaȱsmallȱportionȱofȱplanktonȱhaveȱbeenȱdescribedȱinȱ theȱscientificȱliterature.ȱExpectȱtoȱmakeȱaȱfewȱmistakes,ȱandȱaskȱsomeoneȱelseȱtheirȱopinionȱifȱyouȱareȱnotȱ sure.ȱȱ Aȱtaxonomicȱsuretyȱsystemȱisȱcommonlyȱusedȱwhenȱtryingȱtoȱidentifyȱhowȱconfidentȱyouȱareȱofȱyourȱ data.ȱTheseȱsystemsȱgenerallyȱinvolveȱplacingȱaȱnumberȱbetweenȱ1ȱandȱ3ȱafterȱyourȱidentification.ȱ Numberȱ1ȱmeansȱthatȱyou,ȱandȱmaybeȱaȱcoupleȱofȱotherȱpeople,ȱareȱveryȱhappyȱwithȱyourȱidentification.ȱ Numberȱ2ȱmeansȱthatȱyouȱareȱreasonablyȱsure,ȱbutȱhaveȱnotȱhadȱsomeoneȱelse’sȱopinion,ȱorȱtheyȱareȱnotȱ entirelyȱsure.ȱNumberȱ3ȱmeansȱthatȱyouȱareȱnotȱsureȱatȱall,ȱbutȱthisȱwasȱtheȱclosestȱidentificationȱyouȱ couldȱcomeȱupȱwith.ȱȱ 78 ESTUARINE MONITORING GUIDANCE MANUAL Beȱentirelyȱhonestȱwhenȱgivingȱtheseȱratings.ȱIfȱtheȱdataȱisȱusedȱforȱmanagementȱofȱyourȱestuary,ȱitȱisȱ betterȱthatȱtheȱdecisionȬmakerȱknowsȱhowȱconfidentȱyouȱareȱofȱyourȱdata.ȱTakingȱaȱphotographȱofȱanyȱ thatȱyouȱgiveȱaȱratingȱ3ȱtoȱwouldȱmeanȱthatȱtheseȱidentificationsȱcouldȱbeȱcheckedȱfurtherȱatȱaȱlaterȱdate.ȱ Anȱalternativeȱisȱtoȱsimplyȱcallȱallȱplanktonȱyouȱareȱunsureȱaboutȱ‘unidentifiedȱplankton’,ȱwhichȱwillȱ allowȱyouȱtoȱstillȱcountȱtheȱnumberȱpresent.ȱ What to do: Obtainingȱaȱquantitativeȱphytoplanktonȱsampleȱisȱusuallyȱdoneȱatȱtheȱsameȱtimeȱasȱcollectionȱofȱwaterȱ samplesȱforȱotherȱmeasurements.ȱAȱ200–500–mLȱsampleȱbottleȱisȱfilledȱwithȱwater,ȱobtainedȱfromȱ200ȱ mmȱbelowȱtheȱwaterȱsurface.ȱThisȱisȱbestȱdoneȱatȱtheȱendȱofȱaȱjettyȱorȱotherȱstructure.ȱȱ Thisȱsampleȱisȱreturnedȱtoȱtheȱlaboratoryȱasȱsoonȱasȱpossible,ȱwhereȱ200ȱmLȱisȱpouredȱintoȱaȱ250ȬmLȱ measuringȱcylinder.ȱApproximatelyȱ0.5ȱmLȱofȱLugol’sȱmediaȱisȱaddedȱandȱtheȱsampleȱisȱwellȱshakenȱorȱ stirred.ȱThisȱisȱthenȱleftȱtoȱsettleȱovernight.ȱȱ Theȱtopȱ180ȱmLȱisȱthenȱdecantedȱoffȱcarefully,ȱ usingȱaȱgravityȱsiphon,ȱsoȱthatȱtheȱbottomȱ 20ȱmLȱisȱleftȱundisturbed.ȱThisȱisȱthenȱshakenȱ upȱandȱaȱdropȱofȱtheȱliquidȱisȱplacedȱonȱeachȱ halfȱofȱaȱhaemocytometerȱslideȱandȱaȱcoverȱslipȱ isȱplacedȱoverȱtheȱslide.ȱ ȱ Figure 12—Haemocytometer, general arrangement Theȱhaemocytometerȱisȱaȱglassȱslideȱnormallyȱ usedȱtoȱcountȱbloodȱcells.ȱItȱcanȱbeȱusedȱtoȱ countȱalgalȱcellsȱinȱtheȱsameȱmanner,ȱasȱ discussedȱinȱthisȱguideline.ȱTheȱgeneralȱ structureȱofȱaȱhaemocytometerȱisȱprovidedȱinȱ Figureȱ12.ȱ Placeȱtheȱhaemocytometerȱunderȱtheȱ microscopeȱandȱuseȱtheȱfineȱfocusȱcontrolȱtoȱ gainȱtheȱclearestȱimage.ȱDetailsȱonȱhowȱtoȱdoȱ thisȱareȱgivenȱinȱtheȱguidelineȱGettingȱtoȱknowȱ phytoplanktonȱwithȱaȱnetȱtowȱ(pageȱ75).ȱȱ ȱ Theȱhaemocytometerȱhasȱtwoȱmainȱcountingȱ areasȱ(Figureȱ12).ȱAȱmoreȱdetailedȱdiagramȱofȱaȱ singleȱcountingȱareaȱisȱshownȱarrangementȱinȱ Figureȱ13.ȱEachȱcountingȱareaȱisȱdividedȱintoȱ nineȱequalȱportionsȱofȱoneȱmillimetreȱbyȱ oneȱmillimetre.ȱTheseȱportionsȱareȱreferredȱtoȱasȱ cells.ȱThereȱareȱ18ȱcells.ȱȱ ȱ Figure 13—Counting area of a haemocytometer Theȱalgaeȱpresentȱinȱtheȱcountingȱareasȱareȱ identifiedȱtoȱphylumȱlevelȱwithȱtheȱaidȱofȱtheȱ chartȱprovidedȱinȱFigureȱ14ȱ(aȱlargerȱversionȱisȱ providedȱinȱAppendixȱ4),ȱtheȱpicturesȱprovidedȱ inȱtheȱGettingȱtoȱknowȱphytoplanktonȱwithȱaȱnetȱ towȱguidelineȱandȱanyȱotherȱliteratureȱorȱ photographsȱthatȱyouȱhaveȱaccessȱto.ȱ Theȱchartȱidentifiesȱmarineȱorȱestuarineȱphytoplanktonȱtoȱphyla.ȱThisȱisȱaȱveryȱcoarseȱlevelȱofȱ identification,ȱbutȱthereȱareȱmanyȱveryȱdifferentȱphytoplanktonȱspecies,ȱandȱidentifyingȱthemȱpastȱ phylumȱlevelȱrequiresȱaccessȱtoȱspecialisedȱliterature.ȱUsingȱtheȱflowȱchartȱisȱveryȱeasy.ȱStartingȱatȱtheȱ top,ȱyouȱlookȱatȱtheȱstatementsȱonȱeachȱofȱtheȱpaths.ȱWhichȱoneȱdoesȱtheȱorganismȱyouȱareȱlookingȱatȱ matchȱbest?ȱMoveȱalongȱthatȱbranchȱandȱrepeatȱtheȱprocessȱuntilȱaȱnameȱisȱgiven.ȱCompareȱyourȱobjectȱ 79 ESTUARINE MONITORING GUIDANCE MANUAL toȱtheȱpicturesȱinȱtheȱchart.ȱDoȱtheyȱlookȱanythingȱlikeȱyourȱspecimen?ȱIfȱyouȱcannotȱmakeȱupȱyourȱmindȱ recordȱtheȱorganismȱasȱ‘unidentifiedȱplankton’ȱonȱyourȱdatasheet.ȱ Ifȱthereȱareȱtooȱmanyȱalgaeȱpresentȱtoȱcountȱinȱallȱ18ȱcells,ȱcountȱaȱsmallerȱnumberȱofȱcountingȱcells.ȱInȱ casesȱwhereȱthereȱisȱaȱveryȱhighȱdensityȱofȱalgae,ȱonlyȱoneȱcountingȱareaȱ(comprisingȱnineȱcells),ȱorȱinȱ extremeȱcasesȱonlyȱoneȱcell,ȱneedsȱtoȱbeȱcounted.ȱ Theȱnumberȱandȱtypeȱofȱalgalȱcellsȱinȱeachȱhaemocytometerȱcellȱisȱrecordedȱonȱyourȱdatasheet.ȱTheȱ numberȱofȱcountingȱcellsȱyouȱcountedȱisȱwrittenȱinȱtheȱneighbouringȱcolumn.ȱWhenȱallȱtheȱsamplesȱhaveȱ beenȱcounted,ȱtheȱremainingȱsampleȱbrinesȱshouldȱbeȱdisposedȱofȱbyȱpouringȱthemȱintoȱtheȱbucketȱ containingȱkittyȱlitter.ȱOnceȱtheȱabsorbentȱmaterialȱcanȱcontainȱnoȱmoreȱliquid,ȱitȱshouldȱbeȱbaggedȱandȱ placedȱinȱtheȱwasteȱbinȱforȱdisposalȱtoȱlandfill.ȱȱȱ D at aȱ in t e rpr et at i on ȱ TheȱdataȱfromȱthisȱtypeȱofȱactivityȱisȱoftenȱpresentedȱasȱtheȱnumberȱofȱplanktonȱpresentȱinȱoneȱmLȱofȱ water,ȱorȱtheȱnumberȱofȱplanktonȱfromȱeachȱphylumȱpresentȱinȱoneȱmLȱofȱsample.ȱToȱcalculateȱtheȱ numberȱofȱphytoplanktonȱperȱmLȱorȱtheȱnumberȱofȱeachȱtypeȱperȱmLȱyouȱneedȱtoȱapplyȱtheȱformulaȱ below:ȱ plankton count = no of plankton × 103 no of cells (squares) counted Planktonȱdataȱisȱusuallyȱpresentedȱinȱlistȱorȱgraphȱform,ȱwithȱtheȱclassificationȱlistedȱdownȱoneȱsideȱandȱ theȱcountsȱonȱtheȱotherȱside.ȱComparisonsȱbetweenȱtheȱnumberȱofȱplanktonȱinȱeachȱphylumȱpresentȱ providesȱanȱinformativeȱpictureȱofȱtheȱwaterȱqualityȱinȱyourȱestuary.ȱȱ Theȱtotalȱnumberȱofȱphytoplanktonȱandȱtheȱratiosȱbetweenȱtheȱphylaȱareȱtheȱmostȱusefulȱfigures.ȱAȱhighȱ totalȱcountȱindicatesȱaȱhighȱbiomass,ȱthereforeȱaȱhighȱoxygenȱdemandȱandȱlargeȱamountsȱofȱnutrientsȱinȱ theȱwater.ȱLargeȱnumbersȱofȱblueȬgreenȱalgaeȱsuggestȱthatȱthereȱareȱhighȱconcentrationsȱofȱphosphateȱinȱ theȱwater.ȱLargeȱnumbersȱofȱdiatomsȱsuggestȱthatȱtheȱwaterȱisȱrichȱinȱsilicaȱandȱnutrients.ȱ Playȱaroundȱwithȱtheȱdataȱandȱcombineȱitȱwithȱdataȱfromȱotherȱsitesȱorȱtests.ȱItȱisȱfascinatingȱtoȱseeȱwhatȱ trendsȱorȱcorrelationsȱmayȱbeȱfoundȱthisȱway.ȱ ȱ 80 ESTUARINE MONITORING GUIDANCE MANUAL Figure 14—Plankton identification flow chart 81 ESTUARINE MONITORING GUIDANCE MANUAL Monitoring a seaweed bed Seaweedsȱareȱmacroalgae,ȱmeaningȱthatȱtheyȱformȱlargeȱplantsȱ visibleȱtoȱtheȱnakedȱeye.ȱTheyȱcanȱbeȱred,ȱgreenȱorȱbrown.ȱTheyȱhaveȱ noȱroots,ȱbutȱoftenȱhaveȱholdfasts,ȱaȱcupȬshapedȱappendageȱthatȱ fastensȱontoȱsolidȱsurfaces,ȱincludingȱrocksȱorȱjettyȱpylons.ȱȱ Seaweedȱandȱseaweedȱdistributionȱareȱgoodȱindicatorsȱofȱwaterȱ qualityȱinȱanȱestuary.ȱAsȱseaweedsȱareȱalgae,ȱtheyȱcanȱgrowȱandȱdieȱ offȱveryȱquickly.ȱTheȱcommonȱindicatorsȱofȱestuarineȱhealth— nutrients,ȱpH,ȱturbidity,ȱdissolvedȱoxygenȱandȱsalinity—allȱhaveȱ hugeȱandȱrapidlyȱidentifiableȱimpactsȱonȱseaweedȱcommunities.ȱ Temperatureȱalsoȱhasȱanȱeffectȱonȱtheseȱplants.ȱȱ Inȱcommonȱwithȱmostȱbiologicalȱindicators,ȱtheȱdegreeȱofȱ identificationȱrequiredȱforȱthisȱactivityȱdependsȱonȱwhatȱyouȱwantȱtoȱ useȱtheȱdataȱfor.ȱTheȱeffectsȱofȱlongȬtermȱturbidityȱorȱnutrientsȱmayȱ oftenȱbeȱseenȱbyȱsimplyȱidentifyingȱtheȱseaweedsȱtoȱtheirȱcolour,ȱthenȱ estimatingȱtheȱapproximateȱamountsȱofȱeachȱcolourȱatȱyourȱsite.ȱȱ Activity: 3 Extension Age: High school Time: 3 Why? The more complex types of brown seaweed (macroalgae) may respond to disturbance by disappearing from seaweed beds. Safety: Conducted over deep water (jetty, bridge, boat or wharf) Moreȱdetailedȱidentificationsȱmayȱgiveȱyouȱmoreȱdetailedȱinformation.ȱCatastrophicȱeventsȱsuchȱasȱanȱ anoxicȱeventȱcanȱkillȱentireȱalgalȱcommunities.ȱTheȱstructureȱofȱeachȱplantȱmayȱprovideȱyouȱwithȱanȱ indicationȱofȱhowȱlongȱtheȱcommunityȱhasȱbeenȱgrowing,ȱandȱthereforeȱhowȱlongȱitȱhasȱbeenȱsinceȱoneȱ ofȱtheseȱevents.ȱFlatȱsheetȬlikeȱalgaeȱsuchȱasȱUlvaȱstartȱtoȱgrowȱonȱaȱsiteȱalmostȱimmediatelyȱtheȱeventȱisȱ over.ȱComplexȱalgae,ȱsuchȱasȱCodiumȱspeciesȱorȱtheȱcomplexȱbrownsȱlikeȱkelp,ȱneedȱaȱconsiderableȱtimeȱ afterȱtheȱeventȱbeforeȱtheyȱcoloniseȱtheȱsite.ȱȱ Thisȱactivityȱleavesȱtheȱchoiceȱofȱidentificationȱresolutionȱtoȱtheȱgroup.ȱTheȱguidelineȱdiscussesȱcreatingȱ aȱherbariumȱorȱcollectionȱofȱpressingsȱbeforeȱcommencingȱmonitoring,ȱasȱthisȱallowsȱyouȱtoȱidentifyȱ howȱtimeȱconsumingȱ(orȱnot)ȱdetailedȱidentificationsȱmayȱbeȱatȱyourȱlocation.ȱThisȱpartȱofȱtheȱactivityȱ mayȱneedȱaȱpermitȱfromȱtheȱNPWSȱorȱPIRSA—checkȱbeforeȱstartingȱtheȱproject.ȱ Onceȱpressedȱandȱdried,ȱtheȱspecimensȱcouldȱbeȱtakenȱintoȱtheȱStateȱHerbariumȱ(atȱtheȱAdelaideȱBotanicȱ Gardens)ȱorȱtoȱaȱseaweedȱexpertȱinȱyourȱareaȱforȱdetailedȱidentification.ȱThisȱwouldȱallowȱyouȱtoȱsimplyȱ matchȱtheȱplantsȱyouȱseeȱinȱtheȱfieldȱwithȱthoseȱonȱyourȱnamedȱpressings,ȱwhichȱwouldȱmakeȱregularȱ detailedȱidentificationȱmuchȱquickerȱthanȱotherwise.ȱȱ Severalȱtypesȱofȱequipmentȱforȱmonitoringȱseaweedȱbedsȱareȱsuggested—selectȱtheȱmostȱsuitableȱforȱ yourȱgroupȱandȱmonitoringȱlocation.ȱ What you may need: F or ȱpreparing ȱthe ȱh e r ba r iu m ȱ – bathersȱandȱmaskȱorȱaȱpoleȱwithȱaȱhookȱandȱnetȱatȱoneȱendȱ – bucketȱwithȱwaterproofȱlidȱ – permit(s)ȱfromȱtheȱappropriateȱauthoritiesȱ – sinkȱwithȱplugȱandȱfreshwaterȱ – A3ȱsheetsȱofȱpaper,ȱoneȱorȱtwoȱperȱsampleȱ – severalȱwadsȱofȱnewspaperȱ – waxedȱpaperȱ – woodenȱboardȱaȱlittleȱlargerȱthanȱanȱA3ȱsheetȱ – A3ȱplasticȱsleevesȱ – A3ȱarchȬleverȱfolder.ȱ 82 ESTUARINE MONITORING GUIDANCE MANUAL F or ȱdoingȱth eȱm on it or ing ȱ – GPSȱandȱdetailedȱtopographicȱmapȱ – underwaterȱcamera,ȱnormalȱcameraȱwithȱpolarisingȱlens,ȱorȱcameraȱinȱaȱglassȱaquariumȱȱ – 500Ȭmmȱquadratȱ(optional)ȱ – measuringȱtapeȱ – siteȱherbariumȱ(optional)ȱ – clearȱtransparencyȱfilmȱ – transparencyȱfilmȱmarkedȱwithȱaȱgridȱȱ – permanentȱmarkersȱinȱaȱfewȱcolours.ȱ What to do: S it eȱ selection ȱ Sitesȱareȱselectedȱonȱtheȱbasisȱthatȱtheȱseaweedȱbedsȱshouldȱbeȱobviousȱfromȱtheȱsurface,ȱeasilyȱ accessibleȱandȱfreeȱfromȱanyȱdangerousȱcurrentsȱorȱdeepȱwater.ȱTheȱseaweedȱbedȱmayȱbeȱpresentȱonȱaȱ jettyȱpylon,ȱonȱaȱrockyȱoutcropȱorȱanyȱotherȱhardȱstableȱsurface.ȱȱ Siteȱselectionȱisȱbestȱdoneȱonȱaȱfineȱday.ȱVisitȱeachȱjettyȱandȱpylonȱwithinȱyourȱestuaryȱorȱyourȱsectionȱofȱ theȱestuary.ȱIsȱthereȱanywhereȱthatȱmeetsȱtheȱcriteriaȱabove?ȱIfȱthereȱareȱsites,ȱtakeȱGPSȱlocationsȱandȱ selectȱyourȱfavouriteȱsite.ȱMarkȱtheȱsiteȱclearlyȱonȱyourȱtopographicȱmapȱofȱtheȱarea.ȱIfȱthereȱareȱnoȱsitesȱ meetingȱtheȱaboveȱcriteria,ȱthenȱperhapsȱaȱdifferentȱactivityȱmayȱbeȱmoreȱappropriate.ȱȱ P r ep a r ingȱ aȱh er b ar i umȱ Preparingȱaȱherbariumȱisȱanȱimportantȱstepȱinȱtheȱprocessȱofȱgettingȱtoȱknowȱyourȱseaweedsȱandȱanȱ essentialȱpartȱofȱseaweedȱmonitoring.ȱȱ Selectȱaȱfine,ȱwarm,ȱstillȱdayȱtoȱcollectȱtheȱherbariumȱsamples.ȱTheȱcollectionȱmayȱbeȱdoneȱinȱoneȱofȱtwoȱ ways.ȱSamplesȱcouldȱbeȱcutȱfreeȱofȱtheirȱsubstrateȱandȱcaughtȱinȱaȱsmallȱnetȱusingȱaȱpoleȱwithȱaȱhookȱandȱ netȱattached,ȱorȱtheyȱmayȱbeȱcollectedȱbyȱhandȱwhenȱswimmingȱorȱsnorkelling.ȱȱ Collectingȱbyȱhandȱgivesȱyouȱbetterȱsamples,ȱbutȱtheȱhookȱandȱnetȱmethodȱisȱsaferȱifȱmembersȱofȱyourȱ teamȱdon’tȱknowȱhowȱtoȱswim,ȱtheȱwaterȱqualityȱisȱinappropriateȱforȱswimming,ȱorȱinȱareasȱthatȱareȱ deepȱorȱmayȱexperienceȱstrongȱcurrents.ȱ Onceȱinȱtheȱfield,ȱcollectȱatȱleastȱoneȱsampleȱofȱeachȱseaweedȱyouȱsee.ȱTryȱnotȱtoȱtakeȱtheȱsamplesȱ directlyȱfromȱtheȱsiteȱyouȱintendȱtoȱmonitor.ȱYouȱmayȱneedȱmoreȱthanȱoneȱsampleȱtoȱcomplyȱwithȱyourȱ permitȱconditions.ȱPlaceȱtheseȱsamplesȱintoȱaȱbucketȱcontainingȱaȱlittleȱseawaterȱandȱkeepȱthemȱcool.ȱȱ Onceȱallȱtheȱspecimensȱhaveȱbeenȱcollected,ȱplaceȱtheȱlidȱonȱtheȱbucketȱandȱtakeȱthemȱbackȱtoȱwhereȱyouȱ planȱtoȱpressȱthem,ȱgenerallyȱyourȱhome,ȱmeetingȱhallȱorȱschool.ȱȱ Backȱatȱbase,ȱrinseȱeachȱofȱtheȱsamplesȱoff,ȱremovingȱallȱsand,ȱsaltȱorȱanimalsȱfromȱtheȱfrondsȱandȱfollowȱ theȱprocedureȱbelow:ȱ – plugȱtheȱsinkȱandȱplaceȱaȱsheetȱofȱA3ȱpaperȱinȱitȱȱ – addȱcoolȱwaterȱuntilȱitȱisȱaboutȱ20ȱmmȱdeepȱ – placeȱtheȱfirstȱsampleȱinȱtheȱwaterȱandȱarrangeȱitȱsoȱthatȱitȱlooksȱniceȱandȱrelativelyȱnaturalȱonȱtheȱ pageȱȱ – ensureȱthatȱholdfasts,ȱtheȱshapeȱofȱtheȱfrondsȱandȱanyȱotherȱpossibleȱidentifyingȱcharacteristicsȱ areȱvisibleȱȱ – carefullyȱliftȱaȱcornerȱorȱtheȱpageȱandȱpartiallyȱunplugȱtheȱsink,ȱsoȱthatȱtheȱwaterȱslowlyȱdrainsȱ outȱȱ – ensureȱthatȱtheȱseaweedȱisȱstillȱarrangedȱhowȱyouȱputȱitȱthenȱcarefullyȱliftȱtheȱpaper,ȱwithȱtheȱ algaeȱonȱit,ȱoutȱofȱtheȱsinkȱandȱontoȱaȱfewȱsheetsȱofȱnewspaperȱȱ 83 ESTUARINE MONITORING GUIDANCE MANUAL – ifȱtheȱpaperȱrips,ȱredoȱtheȱactivityȱ – allowȱthisȱsampleȱtoȱdryȱinȱtheȱshadeȱwhileȱtheȱrestȱofȱtheȱsamplesȱareȱpreparedȱ – placeȱaȱsheetȱofȱwaxedȱpaperȱonȱtopȱofȱeachȱsampleȱandȱplaceȱnewȱnewspaperȱaboveȱandȱbelowȱ themȱȱ – stackȱtheȱsamplesȱandȱplaceȱaȱwoodenȱboardȱandȱsomeȱphoneȱbooksȱonȱtopȱ – changeȱtheȱnewspaperȱwheneverȱitȱgetsȱwetȬprobablyȱbeȱeveryȱfewȱhoursȱinitiallyȱthenȱeveryȱ coupleȱofȱdaysȱȱ – onceȱallȱtheȱsamplesȱareȱdry,ȱdustȱanyȱsandȱoffȱȱ – checkȱthatȱtheȱseaweedsȱareȱallȱstuckȱtoȱtheirȱpages,ȱtheyȱhaveȱnotȱdiscolouredȱtheȱpaperȱandȱareȱ relativelyȱflatȱȱ – ifȱtheȱpaperȱisȱdiscoloured,ȱcarefullyȱliftȱoffȱorȱcutȱoutȱtheȱsampleȱandȱstickȱitȱontoȱaȱnewȱsheetȱofȱ paperȱȱ – ifȱtheȱsamplesȱareȱaȱlittleȱloose,ȱstickȱthemȱbackȱontoȱtheirȱpaperȱwithȱacidȬfreeȱglueȱ – onceȱyouȱhaveȱfinished,ȱplaceȱeachȱsheetȱintoȱaȱplasticȱsleeveȱandȱputȱthemȱintoȱaȱlargeȱfolder.ȱ Thisȱwillȱbeȱusedȱregularlyȱduringȱtheȱrestȱofȱthisȱactivity,ȱsoȱensureȱtheȱfolderȱisȱrigid.ȱȱ Identifyingȱeachȱofȱtheȱseaweedsȱinȱtheȱfolderȱisȱanȱimportantȱpartȱofȱthisȱactivity,ȱalthoughȱ identificationȱtoȱspeciesȱisȱnotȱrequired.ȱAskingȱaȱspecialistȱorȱusingȱidentificationȱaidsȱtoȱhelpȱyouȱ identifyȱtheȱspecimensȱtoȱgenusȱlevelȱwouldȱbeȱmoreȱthanȱsufficient.ȱAlternatively,ȱcontactȱReefȱWatchȱ orȱlookȱatȱtheirȱwebsiteȱatȱ<www.reefwatch.asn.au>ȱ M on it or i ngȱm et h od ȱ Monitoringȱyourȱseaweedȱbedȱisȱrelativelyȱsimpleȱonceȱyourȱherbariumȱhasȱbeenȱpreparedȱandȱ identifiedȱtoȱaȱlevelȱyouȱareȱcomfortableȱwith.ȱȱ Selectȱanȱareaȱbetweenȱ0.5–1.0ȱmȱwideȱandȱtheȱsameȱdimensionsȱlengthwise.ȱRecordȱtheȱlocationȱofȱthisȱ usingȱaȱGPSȱandȱmarkȱitȱonȱyourȱtopographicȱmap.ȱWriteȱaȱdescriptionȱofȱtheȱsiteȱonȱtheȱbottomȱofȱtheȱ map.ȱPlaceȱtheȱquadratȱorȱsomeȱotherȱdeviceȱofȱknownȱlengthȱcloseȱtoȱtheȱareaȱyouȱplanȱtoȱphotographȱ andȱtakeȱaȱpictureȱatȱlowȱtideȱfromȱaȱdistance,ȱsoȱthatȱlandmarksȱ(egȱbigȱbouldersȱorȱjettyȱpylons)ȱareȱ visibleȱaroundȱtheȱseaweedȱbed.ȱThisȱphotographȱisȱtoȱhelpȱyouȱfindȱtheȱlocationȱnextȱtimeȱyouȱcomeȱ back.ȱȱ HammeringȱinȱaȱwoodenȱstakeȱorȱsprayȬpaintingȱsomeȱlocatingȱmarksȱmayȱalsoȱbeȱpossible,ȱdependingȱ onȱyourȱlocation.ȱBeȱcarefulȱnotȱtoȱdamageȱtheȱareaȱtoȱbeȱmonitoredȱorȱcreateȱvisualȱpollution.ȱ Onceȱyouȱhaveȱlocatedȱtheȱarea,ȱplaceȱtheȱquadratȱorȱsomethingȱofȱknownȱlengthȱ(aȱscalingȱobject)ȱ immediatelyȱnextȱtoȱit.ȱTakeȱaȱpictureȱofȱtheȱmonitoringȱareaȱandȱtheȱscalingȱobject,ȱensuringȱthatȱtheȱ monitoringȱareaȱandȱtheȱobjectȱtakeȱupȱtheȱentireȱviewingȱareaȱofȱtheȱcamera.ȱȱ Theȱpictureȱcanȱbeȱtakenȱinȱoneȱofȱthreeȱways:ȱ 1 Aȱdisposableȱwaterproofȱcameraȱmayȱbeȱusedȱwithȱitsȱlensȱrestingȱonȱtheȱwaterȱsurfaceȱorȱ submergedȱbelowȱit.ȱ 2 Aȱstandardȱcameraȱfittedȱwithȱaȱpolarisingȱlensȱisȱused.ȱStandingȱonȱaȱjettyȱdirectlyȱaboveȱtheȱ site,ȱcarefullyȱadjustȱtheȱpolarisingȱlensȱsoȱthatȱyouȱcanȱseeȱtheȱmonitoringȱareaȱclearly.ȱȱ 3 Aȱstandardȱcameraȱmayȱbeȱplacedȱinȱaȱglassȱaquarium,ȱwhichȱisȱheldȱpartiallyȱinȱtheȱwater.ȱTheȱ pictureȱisȱtakenȱthroughȱtheȱglassȱbottom.ȱBeȱcarefulȱnotȱtoȱwetȱyourȱcameraȱifȱyouȱuseȱthisȱ method.ȱ Theȱlastȱtwoȱoptionsȱwillȱprobablyȱrequireȱaȱzoomȱlensȱofȱsomeȱvariety,ȱdependingȱonȱtheȱsizeȱofȱyourȱ monitoringȱareaȱandȱtheȱgeographyȱofȱyourȱsampleȱlocation.ȱȱ Theȱmainȱaimȱofȱthisȱexerciseȱisȱtoȱgetȱaȱclearȱphotographȱofȱexactlyȱtheȱsameȱareaȱonȱaȱregularȱbasis.ȱ Withȱthisȱaimȱinȱmind,ȱselectȱtheȱphotographicȱmethodȱmostȱappropriateȱforȱyourȱgroupȱandȱyourȱsite.ȱȱ 84 ESTUARINE MONITORING GUIDANCE MANUAL I nte rpr et ingȱy ou rȱ re su lt s ȱ Onceȱtheȱphotographsȱhaveȱbeenȱprocessed,ȱtheyȱcanȱbeȱanalysedȱusingȱtheȱmethodsȱoutlinedȱinȱtheȱ guidelineȱUsingȱaerialȱphotographsȱtoȱmapȱhabitatȱchange.ȱTheȱphotographsȱtakenȱareȱbasicallyȱhomemadeȱ aerialȱphotographsȱandȱtheyȱwillȱyieldȱaȱsimilarȱrangeȱofȱdata.ȱȱ Classificationȱofȱtheȱseaweedsȱmayȱbeȱasȱsimpleȱasȱtheȱpercentageȱofȱgreen,ȱredȱorȱbrownȱalgae,ȱorȱyouȱ mayȱattemptȱtoȱidentifyȱtheȱalgaeȱinȱtheȱphotographsȱtoȱgenus,ȱusingȱyourȱidentifiedȱherbariumȱ specimensȱorȱotherȱreferenceȱmaterials.ȱȱ TheȱmostȱstableȱareasȱtendȱtoȱbeȱdominatedȱbyȱcomplexȱbrownȱalgaeȱsuchȱasȱSargassumȱandȱEcklonia.ȱ IntermittentlyȱdisturbedȱareasȱmayȱhaveȱCodiumȱ(aȱcomplexȱgreenȱalgae),ȱwhileȱtheȱfilamentousȱandȱ sheetȬtypeȱgreenȱalgaeȱsuchȱasȱEnteromorphaȱandȱUlvaȱareȱrapidȱcolonisersȱafterȱaȱdisturbanceȱorȱindicateȱ highȱnutrientȱconcentrations.ȱRightȱafterȱaȱmajorȱdisturbanceȱaȱfewȱveryȱtiny,ȱfineȱbrownȱfurryȱalgaeȱ suchȱasȱGiffordiaȱmayȱbeȱseen.ȱ Onceȱyouȱhaveȱfinishedȱdoingȱanyȱanalysisȱyouȱwishȱtoȱdoȱonȱtheȱphotographs,ȱsendȱaȱlistȱofȱtheȱ herbariumȱidentifications,ȱaȱcopyȱofȱtheȱphotographsȱandȱtheȱresultsȱofȱanyȱanalysisȱtoȱyourȱWaterwatchȱ coordinatorȱandȱtoȱanyȱagenciesȱthatȱissuedȱpermitsȱforȱtheȱactivity.ȱTheseȱitemsȱofȱdataȱmayȱbeȱ comparedȱwithȱthoseȱcollectedȱbyȱotherȱgroupsȱinȱotherȱlocations,ȱorȱatȱotherȱtimes.ȱȱ Thisȱactivityȱshouldȱbeȱundertakenȱregularly.ȱItȱdependsȱonȱtheȱresourcesȱofȱyourȱgroupȱandȱtheȱaimsȱofȱ theȱprogram,ȱbutȱannuallyȱisȱprobablyȱsufficient.ȱEnsuringȱthatȱtheȱmonitoringȱisȱdoneȱinȱtheȱsameȱ seasonȱeachȱyearȱwillȱreduceȱtheȱeffectȱofȱseasonalȱvariationȱonȱyourȱdata,ȱasȱtheȱopportunisticȱgreenȱ algaeȱinȱparticular,ȱhaveȱwideȱseasonalȱvariationsȱinȱabundance.ȱ U s e f u l r e f e r e n ce s Christianson,ȱIG,ȱClayton,ȱMNȱ&ȱAllender,ȱBMȱ(1981)ȱSeaweedsȱofȱAustralia,ȱReedȱBooks,ȱSydney.ȱ ȱ ȱ ȱ 85 ESTUARINE MONITORING GUIDANCE MANUAL Monitoring an intertidal SAM bed Intertidalȱsubmergedȱaquaticȱmacrophytesȱ(SAMs)ȱareȱvascularȱ plants,ȱwithȱmodifiedȱflowers,ȱseeds,ȱroots,ȱstemsȱandȱleaves.ȱ EstuarineȱSAMsȱincludeȱseagrasses,ȱwidgeonȱgrassesȱandȱwatermats.ȱ Theyȱareȱaquaticȱversionsȱofȱourȱterrestrialȱfloweringȱplants.ȱ MeasuringȱtheȱpercentȱcoverȱofȱSAMȱquantifiesȱtheȱamountȱofȱ macrophytesȱinȱanȱarea,ȱandȱallowsȱchangesȱinȱmacrophyteȱcoverȱtoȱ beȱdetectedȱoverȱtime.ȱTheȱmeasureȱgivesȱanȱindicationȱofȱtheȱstatusȱ andȱstageȱofȱdevelopmentȱofȱaȱmacrophyteȱmeadow.ȱȱ Aȱmacrophyteȱmeadowȱwithȱaȱhighȱpercentageȱofȱcoverȱthatȱdisplaysȱ littleȱvariationȱoverȱtimeȱorȱspaceȱindicatesȱaȱstableȱcommunityȱthatȱisȱ wellȱdeveloped.ȱIfȱchangesȱinȱcoverȱareȱidentified,ȱorȱanȱincreasedȱ amountȱofȱcoatingȱalgaeȱorȱencrustingȱanimalsȱareȱdetectedȱonȱtheȱ leavesȱofȱtheȱplants,ȱtheȱSAMȱbedȱmayȱbeȱsubjectȱtoȱnaturalȱorȱhumanȱ inducedȱstress.ȱȱ Activity: 3 Extension Age: High school Time: 3 Why? SAM (submerged aquatic macrophytes) beds are an essential habitat in estuaries, and are fish nursery areas. Most SAM beds require high light levels to survive, and appropriate inundation, temperature and salinity regimes. Thisȱactivityȱusesȱ500Ȭmmȱquadratsȱplacedȱatȱregularȱintervalsȱalongȱ threeȱtransectsȱwithinȱanȱintertidalȱmacrophyteȱcommunity.ȱAȱrapidȱ assessmentȱofȱmacrophyteȱcoverȱisȱmade.ȱTheȱdominantȱspeciesȱareȱ alsoȱidentified.ȱȱ Safety: Do not use steel star droppers. They corrode and may also be a health and safety problem to people and boats. Dependingȱonȱtheȱlocationȱyouȱwishȱtoȱstudy,ȱaȱpermitȱfromȱtheȱ NativeȱVegetationȱCouncil,ȱTransportȱSAȱorȱPIRSAȱmayȱbeȱrequiredȱ toȱcompleteȱthisȱactivity,ȱparticularlyȱifȱyouȱareȱplanningȱtoȱleaveȱ permanentȱmarkersȱforȱtheȱtransects.ȱȱ Use suncream for this activity, particularly on the back of the neck. Ensure that hats and suitable shoes are worn by all participants. What you may need: – twoȱbrightlyȱpaintedȱgardenȱstakesȱ – rubberȱmalletȱ – quadratȱlabelsȱ – cameraȱwithȱpolarisingȱfilterȱȱ – 50Ȭmȱmeasuringȱtape(s),ȱpreferablyȱmadeȱofȱfibreglassȱȱ – compassȱ – GPSȱȱ – 500ȱmmȱ×ȱ500ȱmmȱquadratȱ(mayȱbeȱsubdividedȱintoȱ100Ȭmmȱsquaresȱwithȱstring)ȱ – aerialȱphotograph,ȱtopographicȱorȱothophotographicȱmapȱ – identificationȱsheetsȱshowingȱlocalȱSAMȱspeciesȱ(theseȱareȱavailableȱforȱsomeȱareasȱfromȱReefȱ WatchȱorȱtheȱCoastȱandȱMarineȱConservationȱBranchȱofȱtheȱDepartmentȱforȱConservationȱandȱ Heritage).ȱ Only undertake this activity at low tide. To ensure good results: Seagrassȱmeadowsȱexperienceȱseasonalȱandȱclimaticȱtrendsȱinȱtheirȱpercentageȱcover.ȱAtȱleastȱthreeȱyearsȱ ofȱdataȱ(preferablyȱmore)ȱareȱrequiredȱtoȱtrackȱunusualȱchanges.ȱ What to do: Selectȱaȱ50mȱ×ȱ50ȱmȱsiteȱusingȱanȱaerialȱphotograph,ȱorthophotographicȱmapȱorȱbyȱwalkingȱacrossȱtheȱ site.ȱTheȱsiteȱneedsȱtoȱbeȱrelativelyȱhomogenousȱwithȱnoȱobviousȱsandbanksȱorȱchanges.ȱEnsureȱthatȱitȱ appearsȱtoȱbeȱrepresentativeȱofȱtheȱSAMȱcommunityȱatȱthatȱlocationȱandȱisȱnotȱdifficultȱtoȱrevisit.ȱȱ 86 ESTUARINE MONITORING GUIDANCE MANUAL OnceȱaȱsiteȱisȱselectedȱrecordȱitsȱpositionȱusingȱaȱGPSȱandȱbyȱtakingȱcompassȱbearingsȱfromȱprominentȱ landȱfeatures.ȱRecordȱtheseȱmeasurementsȱandȱplotȱtheȱlocationȱonȱtheȱaerialȱphotographȱorȱtopographicȱ map.ȱThisȱwillȱassistȱwithȱfindingȱtheȱsiteȱwhenȱrevisiting.ȱȱ Threeȱtransectsȱareȱrequired.ȱEachȱtransectȱshouldȱrunȱperpendicularȱtoȱtheȱseaȱandȱbeȱ50Ȭmȱlong.ȱAllȱ quadratsȱshouldȱbeȱplacedȱonȱtheȱrightȬhandȱsideȱofȱtheȱtransects,ȱsoȱonlyȱwalkȱonȱtheȱleftȱhandȱside.ȱ Markȱtheȱmiddleȱtransectȱbyȱdrivingȱaȱgardenȱstakeȱintoȱtheȱgroundȱatȱeitherȱend,ȱleavingȱapproximatelyȱ 100ȱmmȱstickingȱout.ȱTakeȱaȱGPSȱreadingȱatȱeachȱpegȱandȱattachȱaȱsiteȱmarkerȱtagȱtoȱeachȱmarker.ȱȱ Theȱotherȱtwoȱtransectsȱshouldȱrunȱparallelȱto,ȱbutȱ25Ȭmȱtoȱtheȱleftȱandȱrightȱof,ȱtheȱmiddleȱtransect.ȱ Theseȱshouldȱbeȱunmarked,ȱandȱwillȱneedȱmeasuringȱeachȱvisit,ȱorȱGPSȱlocationsȱrecordedȱtoȱlocateȱtheirȱ startȱandȱfinishȱpoints.ȱȱ Alwaysȱstartȱmonitoringȱanȱhourȱorȱsoȱbeforeȱlowȱtide.ȱAtȱtheȱbeginningȱofȱtheȱfirstȱquadrat,ȱplaceȱaȱ500ȱ mmȱ×ȱ500ȱmmȱquadratȱtoȱtheȱrightȱhandȱsideȱofȱtheȱtransect.ȱPlaceȱaȱquadratȱlabelȱnextȱtoȱtheȱquadratȱ andȱphotographȱtheȱquadratȱsoȱthatȱminimalȱglareȱinterferesȱwithȱtheȱphotographȱ(turnȱtheȱpolarisingȱ filterȱuntilȱyouȱgetȱnoȱreflectionsȱfromȱtheȱwater).ȱȱ Youȱmayȱestimateȱtheȱpercentageȱcoverȱofȱmacrophytesȱinȱtheȱfieldȱorȱatȱyourȱdeskȱonceȱtheȱphotosȱhaveȱ beenȱdeveloped.ȱThisȱmayȱbeȱeasierȱifȱyouȱsubdivideȱyourȱquadrat.ȱOnceȱyouȱhaveȱobtainedȱanȱestimateȱ ofȱmacrophyteȱcover,ȱrecordȱyourȱresultȱontoȱtheȱdataȱsheet.ȱȱ Onceȱtheȱphotographȱhasȱbeenȱtaken,ȱidentifyȱandȱrecordȱtheȱspeciesȱofȱmacrophyteȱpresentȱinȱtheȱ quadrat.ȱIfȱthereȱisȱmoreȱthanȱoneȱspeciesȱpresent,ȱanȱestimateȱofȱhowȱmuchȱofȱtheȱtotalȱcoverȱeachȱ speciesȱcontributesȱwillȱbeȱrequired.ȱIfȱyouȱareȱunableȱtoȱidentifyȱtheȱmacrophyteȱspecies,ȱtakeȱaȱ specimenȱ(ifȱyouȱhaveȱtheȱappropriateȱpermit)ȱorȱtakeȱaȱphotographȱofȱit.ȱȱ Repeatȱtheȱaboveȱprocedureȱatȱtheȱbeginning,ȱmiddleȱandȱendȱofȱallȱthreeȱtransects.ȱThisȱactivityȱshouldȱ beȱrepeatedȱannuallyȱatȱtheȱsameȱtimeȱofȱyear,ȱtoȱminimiseȱtheȱimpactȱofȱseasonalȱvariations.ȱȱ Dataȱfromȱthisȱactivityȱmayȱbeȱpresentedȱasȱaverageȱpercentageȱcoverȱforȱtheȱsite,ȱorȱdominantȱspeciesȱ forȱeachȱsite.ȱPresentingȱtheȱdataȱasȱaȱgraphȱorȱseriesȱofȱgraphsȱisȱaȱgoodȱmethodȱforȱassessingȱtrendsȱ overȱtimeȱatȱtheȱsiteȱorȱbetweenȱsites.ȱSAMȱbedsȱexperienceȱseasonalȱandȱclimaticȱtrendsȱinȱtheirȱ percentageȱcover.ȱAtȱleastȱthreeȱyearsȱofȱdataȱ(preferablyȱmore)ȱareȱrequiredȱtoȱtrackȱunusualȱchanges.ȱ ȱ ȱ ȱ 87 ESTUARINE MONITORING GUIDANCE MANUAL Monitoring submerged SAM beds InȱshelteredȱestuariesȱthatȱsupportȱSAMȱ(submergedȱaquaticȱ macrophyte)ȱbeds,ȱaȱstableȱpopulationȱofȱtheseȱplantsȱisȱtheȱhallmarkȱ ofȱaȱhealthyȱestuary.ȱȱ SAMȱbedsȱcontributeȱtoȱtheȱestuaryȱinȱseveralȱways.ȱTheyȱprovideȱ foodȱforȱseveralȱtypesȱofȱwaterfowlȱandȱaȱnurseryȱforȱjuvenileȱfish.ȱInȱ death,ȱtheȱplantsȱprovideȱhugeȱquantitiesȱofȱdecomposedȱmatterȱasȱ leavesȱdie,ȱandȱsomeȱaquaticȱinvertebratesȱuseȱthisȱdetritusȱasȱtheirȱ mainȱfoodȱsource.ȱDuringȱtheȱwarmerȱgrowingȱseason,ȱSAMsȱsupplyȱ oxygenȱtoȱtheȱwater.ȱTheȱSAMȱbedsȱlockȱupȱnutrientsȱduringȱtheȱ summer,ȱandȱasȱtheyȱdieȱoffȱinȱautumnȱtheyȱslowlyȱreleaseȱtheseȱbackȱ intoȱcolderȱwaterȱthatȱwillȱnotȱsupportȱlargeȱplanktonȱblooms.ȱ SAMȱbedsȱrequireȱhighȱlightȱlevelsȱtoȱsurvive,ȱandȱappropriateȱ inundation,ȱtemperatureȱandȱsalinityȱregimes.ȱ InȱSouthȱAustraliaȱtheȱCoastȱandȱMarineȱBranchȱofȱtheȱDepartmentȱ forȱEnvironmentȱandȱHeritageȱhasȱdevelopedȱmapsȱofȱsubmergedȱ SAMȱbeds,ȱderivedȱfromȱanalysingȱaerialȱsurveys.ȱTheseȱareȱ availableȱforȱmanyȱpartsȱofȱtheȱstate,ȱfromȱtheȱSouthȱAustralianȱnodeȱ ofȱtheȱCoastalȱAtlas,ȱaccessedȱthroughȱtheȱinternetȱonȱ <www.atlas.sa.gov.au>.ȱȱ Activity: 3 Extension Age: Adult groups Time: 4 Why? SAM (submerged aquatic macrophytes) beds are an essential habitat in estuaries, and are fish nursery areas. Most SAM beds require high light levels to survive, and appropriate inundation, temperature and salinity regimes. Safety: This is a deep water activity. Itȱisȱnotȱalwaysȱeasyȱtoȱseparateȱsomeȱtypesȱofȱseaweedȱ(algae)ȱfromȱSAMs,ȱandȱaerialȱsurveysȱmayȱmissȱ smallȱbeds.ȱToȱimproveȱtheȱaccuracyȱofȱtheseȱmaps,ȱgroundtruthingȱisȱused,ȱwhereȱtheȱsitesȱidentifiedȱ fromȱtheȱaerialȱphotographsȱareȱvisited,ȱandȱsampled,ȱtoȱconfirmȱthatȱtheyȱareȱindeedȱSAMȱbeds.ȱ GroundtruthingȱsubmergedȱSAMȱbedsȱinȱyourȱestuaryȱprovidesȱusersȱofȱyourȱdataȱwithȱinformationȱonȱ theȱaccuracyȱofȱtheȱlargerȱscaleȱaerialȱmapping,ȱasȱwellȱasȱprovidingȱinformationȱonȱtheȱlocationȱofȱ smallerȱbeds.ȱȱ IfȱyouȱwishȱtoȱsampleȱSAMȱbeds,ȱyouȱmayȱneedȱaȱpermitȱtoȱcollectȱnativeȱvegetationȱfromȱtheȱNativeȱ VegetationȱCouncil.ȱTheseȱpermitsȱareȱissuedȱbyȱtheȱNPWS.ȱ What you may need: – seaworthyȱboatȱwithȱadequateȱsafetyȱandȱrunningȱequipmentȱ – GPSȱ – mapȱshowingȱlocationsȱofȱSAMȱbedsȱinȱyourȱestuaryȱ – aȱlongȬhandledȱrakeȱ – identificationȱsheetsȱshowingȱlocalȱSAMȱspeciesȱ(theseȱareȱavailableȱforȱsomeȱareas,ȱfromȱReefȱ WatchȱorȱtheȱCoastȱandȱMarineȱBranch)ȱ – plasticȱzipȱbagsȱforȱspecimens,ȱwithȱaȱmarkerȱpenȱtoȱlabelȱthemȱ – polarisedȱsunglasses,ȱswimmingȱgogglesȱorȱaȱclearȬbottomedȱcontainerȱforȱviewingȱtheȱSAMȱ bedsȱ – groundtruthingȱdatasheet.ȱ To ensure good results: Goȱoutȱonȱtheȱcalmestȱdayȱyouȱcan,ȱatȱaȱreasonablyȱlowȱtide.ȱThisȱwillȱallowȱyouȱtoȱseeȱintoȱtheȱwaterȱ moreȱclearly.ȱ Undertakeȱthisȱactivityȱatȱaȱsimilarȱtimeȱofȱyearȱ(preferablyȱsummer)ȱtoȱminimiseȱtheȱimpactȱofȱseasonalȱ variationȱifȱyouȱvisitȱtheȱsitesȱrepeatedlyȱtoȱmonitorȱtheirȱhealth.ȱSomeȱspeciesȱofȱSAMsȱdieȱbackȱinȱ winter.ȱȱ 88 ESTUARINE MONITORING GUIDANCE MANUAL What to do: Beforeȱgoingȱoutȱinȱtheȱboat,ȱmarkȱtheȱlocationsȱyouȱwishȱtoȱvisitȱonȱyourȱmap,ȱandȱwriteȱtheirȱmapȱ coordinatesȱnextȱtoȱthem.ȱRecordȱtheȱproposedȱlocationsȱonȱyourȱdatasheetȱasȱwell.ȱ Proceedȱtoȱtheȱfirstȱlocationȱonȱyourȱsheet.ȱOnȱapproachingȱaȱSAMȱbed,ȱSLOWȱDOWN.ȱEnsureȱyouȱwillȱ notȱdamageȱtheȱbedȱwithȱyourȱpropellerȱitselfȱorȱfromȱtheȱperturbationȱitȱmayȱcause.ȱAreȱyouȱatȱtheȱ locationȱyouȱwereȱheadingȱtowards,ȱorȱhaveȱyouȱfoundȱaȱsmallȱbedȱthatȱisȱnotȱmarkedȱonȱyourȱmap?ȱ FloatȱoverȱtheȱSAMȱbedȱinȱyourȱboat.ȱYouȱwillȱgetȱaȱclearerȱviewȱbyȱusingȱpolarisedȱsunglasses,ȱorȱbyȱ puttingȱonȱyourȱswimmingȱmaskȱandȱsubmergingȱyourȱheadȱ(beȱcarefulȱnotȱtoȱfallȱinȱtheȱwater),ȱorȱbyȱ lookingȱthroughȱaȱclearȬbottomedȱcontainerȱ(oldȱaquariumȱorȱsimilar).ȱ Recordȱyourȱlocation,ȱandȱtheȱsizeȱandȱshapeȱofȱtheȱSAMȱbed.ȱSlowlyȱcircumnavigateȱtheȱbedȱandȱtakingȱ readingsȱfromȱtheȱGPSȱtoȱdetermineȱtheȱshape.ȱ CanȱyouȱidentifyȱtheȱtypeȱofȱSAM?ȱIfȱyouȱareȱunsureȱofȱtheȱspecies,ȱuseȱyourȱrakeȱtoȱGENTLYȱfishȱupȱaȱ smallȱspecimen,ȱbagȱit,ȱlabelȱit,ȱandȱreturnȱitȱtoȱshoreȱforȱidentification.ȱIfȱyouȱcanȱseeȱwhatȱspeciesȱitȱis,ȱ justȱrecordȱtheȱspeciesȱonȱtheȱdatasheet.ȱ HowȱhealthyȱdoesȱtheȱSAMȱappear?ȱIfȱyouȱhaveȱvisitedȱthisȱbedȱbefore,ȱisȱitȱanyȱdifferent?ȱNoteȱtheȱ locationȱofȱanyȱerosionȱscarpsȱorȱblowoutsȱusingȱtheȱGPS.ȱRecordȱwhetherȱthereȱisȱmuchȱepiphyticȱ growthȱ(visibleȱasȱaȱsoftȱbrownishȱfuzz)ȱonȱtheȱbladesȱofȱtheȱSAM.ȱ Proceedȱtoȱtheȱnextȱbedȱandȱrepeatȱtheȱprocess.ȱ Onȱyourȱreturnȱtoȱbase,ȱidentifyȱallȱsamples,ȱorȱcontactȱyourȱWaterwatchȱcoordinatorȱtoȱhelpȱyouȱfindȱ someoneȱwhoȱcanȱidentifyȱthemȱforȱyou.ȱOnȱyourȱSAMȱbedȱmapȱorȱaȱtopographicȱmap,ȱtryȱtoȱdrawȱinȱ anyȱnewȱbedȱlocations,ȱorȱanyȱotherȱchangesȱasȱaccuratelyȱasȱyouȱcan.ȱȱ IfȱyouȱnoticeȱaȱregularȱreductionȱinȱtheȱsizeȱofȱSAMȱbeds,ȱorȱincreasingȱnumbersȱofȱblowoutsȱandȱ propellerȱscarsȱoverȱseveralȱyears,ȱtalkȱtoȱyourȱWaterwatchȱcoordinator,ȱwhoȱcanȱputȱyouȱinȱcontactȱwithȱ theȱappropriateȱpeople.ȱ ȱ ȱ ȱ 89 ESTUARINE MONITORING GUIDANCE MANUAL How continuous is the fringing vegetation? Riparianȱvegetationȱisȱimportantȱinȱestuarineȱsituations.ȱRiparianȱ vegetationȱgrowsȱalongsideȱtidalȱwatercoursesȱaboveȱtheȱhighȬtideȱ mark,ȱwhereȱonlyȱkingȱtides,ȱfloodwatersȱorȱrunoffȱregularlyȱ inundateȱit.ȱInȱtheȱupperȱtidalȱfreshwaterȱreachesȱthisȱvegetationȱisȱ oftenȱdistinctlyȱdifferentȱfromȱtheȱsurroundingȱvegetation,ȱoftenȱ includingȱemergentȱmacrophytesȱdueȱtoȱtheȱbetterȱavailabilityȱofȱ freshwater.ȱHowever,ȱfurtherȱdownȱtheȱestuaryȱtheȱvegetationȱ becomesȱlessȱdistinctȱfromȱtheȱsupratidalȱvegetation,ȱwithȱtheȱ vegetationȱbandsȱoftenȱcomprisingȱvariousȱtypesȱofȱsamphire.ȱȱ Riparianȱvegetationȱaidsȱinȱpreventingȱerosion,ȱprovidesȱhabitatȱforȱ smallȱbirdsȱorȱotherȱanimals,ȱandȱcleansȱstormwaterȱrunoffȱbeforeȱitȱ reachesȱtheȱestuarineȱsystem.ȱȱ MuchȱofȱAustralia’sȱriparianȱvegetationȱisȱdegraded.ȱPreviousȱ clearingȱforȱurban,ȱindustrialȱandȱagriculturalȱdevelopmentȱhasȱ reducedȱtheȱqualityȱofȱriparianȱvegetationȱwhileȱweedȱandȱferalȱ animalȱinvasionsȱhaveȱtakenȱoverȱtheȱareasȱstillȱleftȱinȱtheirȱnaturalȱ state.ȱȱ Considerableȱcommunityȱandȱgovernmentȱeffortȱhasȱbeenȱspentȱ tryingȱtoȱrestoreȱtheseȱestuarineȱriparianȱzonesȱoverȱtheȱpastȱ10ȱtoȱ20ȱ years.ȱSomeȱAdelaideȱcouncilsȱhaveȱbuiltȱlargeȱareasȱofȱartificialȱ wetlandsȱtoȱhelpȱrecreateȱaȱsmallȱportionȱofȱthisȱhabitat,ȱandȱtheseȱareȱ usedȱtoȱtreatȱstormwaterȱbeforeȱitȱentersȱtheȱBarkerȱInletȱandȱtheȱPortȱ Riverȱestuary.ȱȱ Activity: 3 Extension Age: High school Time: 2 Why? Width and continuity of riparian vegetation is related to water quality, particularly water clarity. Other important indicators examined in this activity are weediness and erosion. Safety: Winter months are normally the best time of the year to conduct assessments as the climate is milder and the risks of encountering snakes is lower. Always ensure that boots, long pants and a hat are worn by all participants. Water bottles or regular refreshment stops are also recommended. Thisȱactivityȱaimsȱtoȱprovideȱaȱrapidȱassessmentȱofȱriparianȱ vegetationȱalongȱaȱ100Ȭmȱstretchȱofȱestuarineȱcreekȱandȱgiveȱanȱ overviewȱofȱtheȱconditionȱofȱremainingȱriparianȱvegetationȱwithinȱtheȱtidalȱarea.ȱTheȱactivityȱusesȱaerialȱ photosȱtoȱselectȱhomogenousȱstretchesȱofȱriparianȱvegetationȱtoȱhelpȱreduceȱtheȱamountȱofȱfieldwork.ȱ Representativeȱsitesȱonȱtheȱgroundȱareȱassessed.ȱFiveȱkeyȱindicatorsȱofȱriparianȱvegetationȱdisturbanceȱ areȱexaminedȱtoȱprovideȱanȱindexȱscoreȱofȱriparianȱhealth.ȱȱ What you may need: – currentȱaerialȱphotosȱofȱareaȱtoȱbeȱexaminedȱinȱanȱappropriateȱscaleȱȱ – topographicȱmapȱofȱtheȱareaȱinȱaȱsimilarȱscaleȱtoȱtheȱphotographsȱȱ – 50Ȭmȱtapeȱmeasureȱ – oneȬmetreȱmeasuringȱstickȱorȱsimilarȱobjectȱȱ – cameraȱ – riparianȱvegetationȱdatasheet.ȱ What to do: Thisȱactivityȱcanȱbeȱperformedȱonȱjustȱyourȱregularȱsampleȱsite,ȱaȱnumberȱofȱsites,ȱaȱsingleȱtidalȱcreekȱorȱ onȱtheȱentireȱestuary.ȱTheȱactivityȱdescribedȱhereȱdetailsȱhowȱyouȱwouldȱassessȱtheȱriparianȱvegetationȱ ofȱtheȱentireȱlengthȱofȱaȱtidalȱcreek.ȱHowever,ȱitȱisȱsimpleȱtoȱcutȱitȱdownȱtoȱaȱ100Ȭmȱsiteȱbyȱperformingȱ theȱfieldworkȱsectionȱonȱyourȱregularȱwaterȱsamplingȱlocations,ȱorȱtoȱgraduallyȱassessȱdifferentȱsitesȱ untilȱyouȱhaveȱcoveredȱyourȱentireȱestuary.ȱȱ Homogenousȱstreamȱunitsȱareȱsectionsȱofȱstreamȱthatȱhaveȱconsistentȱcharacteristicsȱalongȱtheirȱlength.ȱ Thisȱmethodȱassumesȱthatȱthereȱisȱlittleȱvariationȱinȱtheȱstructureȱofȱtheȱriparianȱvegetationȱwithinȱeachȱ 90 ESTUARINE MONITORING GUIDANCE MANUAL unit.ȱThisȱmeansȱthatȱanȱassessmentȱofȱtheȱentireȱriparianȱzoneȱmayȱbeȱmadeȱbyȱvisitingȱoneȱsiteȱwithinȱ eachȱunit.ȱȱ Theȱnumberȱofȱhomogenousȱstreamȱunitsȱforȱeachȱstreamȱwillȱvaryȱconsiderably,ȱdependingȱonȱtheȱ natureȱofȱtheȱstreamȱandȱitsȱvegetation.ȱUsingȱaerialȱphotosȱandȱtopographicȱmap,ȱdivideȱyourȱestuaryȱ banksȱintoȱhomogenousȱunitsȱusingȱtheȱfollowingȱmethod:ȱȱ 1 Divideȱtheȱestuaryȱatȱeachȱbendȱandȱatȱanyȱtributaryȱjunctionsȱ 2 Divideȱtheseȱsectionsȱfurtherȱwhereȱtheȱwidthȱofȱtheȱriparianȱzoneȱchangesȱorȱdiscontinuesȱ 3 Ifȱlandȱusesȱchange,ȱdivideȱtheȱcurrentȱsectionsȱfurtherȱsoȱdifferentȱlandȱusesȱareȱinȱdifferentȱ unitsȱ 4 Ensureȱthatȱeachȱofȱtheȱdivisionsȱisȱmarkedȱontoȱtheȱtopographicȱmapȱorȱaȱtransparencyȱsheetȱ tapedȱontoȱit.ȱȱ Eachȱstreamȱunitȱneedsȱtoȱbeȱgreaterȱthanȱ300ȱmȱlong.ȱSelectȱaȱrepresentativeȱ100Ȭmȱlengthȱofȱeachȱ streamȱunitȱthatȱisȱaccessibleȱtoȱyourȱgroup.ȱȱ Theȱremainingȱpartȱofȱthisȱactivityȱcomprisesȱtheȱfieldworkȱthatȱisȱconductedȱonȱeachȱ100Ȭmȱstretch.ȱ Eachȱstretchȱneedsȱtoȱbeȱvisited,ȱandȱtheȱfollowingȱparametersȱassessed.ȱAlwaysȱobtainȱpermissionȱfromȱ landholdersȱifȱtravellingȱonȱprivateȱpropertyȱisȱrequired.ȱ Markȱoutȱaȱ100Ȭmȱstretchȱofȱriparianȱvegetationȱfromȱaȱpointȱthatȱisȱeasyȱtoȱrelocateȱonȱfutureȱvisits.ȱ MeasureȱtheȱwidthȱofȱtheȱriparianȱvegetationȱonȱtheȱbankȱbyȱrunningȱaȱtapeȱmeasureȱfromȱtheȱhighȬ waterȱmarkȱtoȱtheȱouter,ȱorȱlandward,ȱedgeȱofȱtheȱriparianȱvegetation.ȱRecordȱthisȱonȱyourȱdatasheet.ȱIfȱ mangrovesȱareȱpresent,ȱmeasureȱtheȱwidthȱofȱtheȱmangroveȱcommunityȱseparatelyȱandȱrecordȱonȱtheȱ dataȱsheet.ȱȱ Lookȱatȱtheȱextentȱofȱtheȱremainingȱriparianȱvegetationȱandȱestimateȱtheȱpercentȱremainingȱbyȱwalkingȱ alongȱtheȱlandwardȱedgeȱofȱtheȱvegetationȱandȱlookingȱtowardsȱtheȱestuary.ȱIfȱaȱbankȱhasȱaȱwellȬ vegetatedȱriparianȱzoneȱthatȱisȱcontinuousȱandȱconsistent,ȱitȱhasȱexcellentȱcontinuity.ȱAreȱthereȱanyȱgapsȱ inȱtheȱvegetation,ȱorȱisȱitȱoneȱlongȱbelt?ȱRecordȱtheȱnumberȱandȱwidthȱofȱbreaksȱinȱtheȱriparianȱ vegetationȱandȱuseȱthisȱinformationȱtoȱcalculateȱpercentageȱcover.ȱNoteȱgrazingȱorȱploughingȱofȱtheȱ riparianȱvegetation.ȱDescribeȱtheȱexistingȱvegetationȱandȱidentifyȱit,ȱifȱyouȱcan.ȱ Lookȱaroundȱforȱweedȱspecies.ȱAreȱtheȱplantsȱmainlyȱweedsȱorȱareȱtheyȱnatives?ȱWhatȱnoxiousȱweedȱ speciesȱareȱpresent?ȱNoteȱyourȱobservationsȱonȱyourȱdatasheet.ȱ Assessȱtheȱseverityȱofȱerosionȱonȱtheȱbank.ȱCircleȱtheȱcategoryȱonȱtheȱdatasheetȱthatȱbestȱdescribesȱtheȱ extentȱofȱtheȱerosionȱatȱthisȱsite.ȱȱ Takeȱaȱphotoȱofȱtheȱriparianȱvegetationȱbyȱstandingȱatȱoneȱendȱofȱyourȱtransectȱonȱtheȱlandwardȱside,ȱ lookingȱalongȱyourȱtransect.ȱEnsureȱaȱmeasuringȱstickȱisȱplacedȱinȱanȱeasilyȱrepeatableȱlocationȱ(nextȱtoȱaȱ distinctȱtree,ȱforȱexample).ȱRepeatedȱphotosȱfromȱthisȱpointȱmayȱbeȱscaledȱagainstȱtheȱmeasuringȱstickȱ andȱanyȱchangesȱwillȱbeȱeasyȱtoȱobserve.ȱ Recordȱanyȱotherȱvisibleȱsignsȱofȱdegradationȱincludingȱlitterȱorȱdumping.ȱDescribeȱtheȱpresenceȱandȱ extentȱofȱrevegetationȱeffortsȱorȱotherȱenvironmentalȱworksȱandȱtheȱpresenceȱandȱlocationȱofȱdrainageȱ outletsȱorȱotherȱmanȱmadeȱstructures.ȱ Theȱdatasheetȱprovidesȱaȱmethodȱofȱscoringȱeachȱsite.ȱThisȱisȱanȱindexȱscoreȱforȱtheȱhealthȱofȱtheȱ vegetation,ȱbasedȱonȱtheȱamountȱofȱhumanȱdisturbance.ȱTheseȱscoresȱmayȱbeȱcomparedȱwithȱscoresȱfromȱ otherȱsitesȱtoȱhelpȱdetermineȱwhereȱtheȱmostȱorȱleastȱdegradedȱsitesȱare.ȱȱ Ifȱyourȱgroupȱassessesȱanȱentireȱtidalȱcreek,ȱaȱmapȱcouldȱbeȱdevelopedȱshowingȱtheȱlocationsȱofȱhigherȱ andȱlowerȱscoredȱareas.ȱSuchȱaȱmapȱwouldȱprovideȱlocalȱLandcareȱgroupsȱorȱtheȱlocalȱcouncilȱwithȱ assistanceȱinȱidentifyingȱandȱprioritisingȱanyȱenvironmentalȱimprovementȱprogramsȱtheyȱmayȱbeȱ planning.ȱTheȱphotopointȱphotographsȱtakenȱoverȱseveralȱyearsȱareȱalsoȱusefulȱinȱtrackingȱtheȱprogressȱ ofȱrevegetationȱefforts.ȱ 91 ESTUARINE MONITORING GUIDANCE MANUAL Monitoring mangrove leaf/twig litter production InȱSouthȱAustralia,ȱonlyȱoneȱspeciesȱofȱmangroveȱoccurs.ȱThisȱisȱ Avicenniaȱmarinaȱ(itsȱnameȱrefersȱtoȱitsȱseaȬgoingȱhabit).ȱItȱisȱ commonlyȱcalledȱtheȱWhiteȱ(orȱGrey)ȱMangrove,ȱdueȱtoȱtheȱcolourȱofȱ theȱundersideȱofȱitsȱleaves.ȱItȱisȱtheȱmostȱcoldȱtolerantȱofȱallȱ mangroves,ȱandȱisȱalsoȱoneȱofȱtheȱfastestȱcolonisingȱspecies.ȱȱ Tropicalȱareas,ȱsuchȱasȱtheȱNorthernȱTerritory,ȱmayȱhaveȱupȱtoȱ36ȱ speciesȱofȱmangroveȱinȱlargeȱmangalsȱorȱmangroveȱforests.ȱȱ Mangroveȱlitterȱproductionȱrefersȱtoȱtheȱsheddingȱofȱleaves,ȱtwigs,ȱ flowers,ȱseedsȱorȱotherȱvegetativeȱmatterȱbyȱaȱmangroveȱtree.ȱThisȱ mayȱbeȱcausedȱbyȱage,ȱstressȱorȱmechanicalȱfactorsȱ(egȱwind,ȱtidesȱorȱ floods).ȱȱ Measuringȱtheȱlitterȱproductionȱofȱindividualȱtreesȱmayȱmonitorȱtheȱ healthȱofȱaȱmangroveȱforest.ȱAȱhealthyȱsystemȱshouldȱhaveȱaȱhighȱ and/orȱstableȱmonthlyȱlitterȱproduction,ȱalthoughȱthisȱmayȱhaveȱlargeȱ seasonalȱvariationsȱ(May,ȱ1999).ȱDecliningȱleafȱlitterȱproductionȱoverȱ aȱperiodȱofȱyears,ȱorȱsuddenȱcopiousȱlitterȱproductionȱmayȱindicateȱ thatȱtheȱcommunityȱisȱunderȱstress.ȱȱ Theȱamountȱofȱreproductiveȱmaterialȱmayȱalsoȱbeȱusedȱasȱaȱmeasureȱ ofȱhealth,ȱasȱreproductionȱeffortȱchangesȱifȱplantsȱareȱstressed.ȱ PropagulesȱfromȱtheȱWhiteȱMangroveȱtakeȱupȱtoȱtwoȱyearsȱtoȱform,ȱ thenȱfallȱfromȱtheȱparentȱplant,ȱdependingȱonȱclimate,ȱsoȱaȱ downwardȱtrendȱinȱtheȱnumbersȱofȱpropagulesȱmayȱtakeȱmanyȱyearsȱ toȱdetect.ȱȱ Litterȱfallȱfromȱmangroveȱtreesȱrepresentsȱaȱsignificantȱpartȱofȱtheȱnetȱ primaryȱproductivityȱofȱaȱmangroveȱforest,ȱalthoughȱaȱconsiderableȱ amountȱofȱextraȱdetritusȱisȱbroughtȱinȱwithȱeachȱtide.ȱȱ Activity: 3 Extension Age: High school Time: 3 Why? Leaf and flower production is an indicator of mangrove health. The litter from mangroves is an important part of the detrital cycle and a reduction may affect fauna. Safety: Snakes may pose danger during the summer months, especially the drier approaches to the mangroves. Always wear suitable clothing (strong shoes and long trousers) and work in pairs to minimise the risk. Keep a careful eye on the litter when drying it in the oven, to ensure it does not catch alight. Heat risks apply. Litterȱfallȱisȱmeasuredȱbyȱinstallingȱleafȱlitterȱtrapsȱalongȱaȱtransectȱinȱaȱmangroveȱcommunity.ȱTrappedȱ litterȱisȱcollectedȱandȱsortedȱintoȱdifferentȱcategories,ȱovenȱdriedȱandȱthenȱweighed.ȱTheȱdryȱweightȱofȱ eachȱcategoryȱprovidesȱaȱmeasureȱofȱtheȱoverallȱlitterȱproductivityȱofȱtheȱmangal.ȱȱ AȱpermitȱfromȱPIRSAȱwillȱbeȱrequiredȱforȱthisȱactivity.ȱIfȱanyȱaquacultureȱorȱminingȱleasesȱareȱheldȱoverȱ theȱlandȱyouȱareȱworkingȱon,ȱyouȱwillȱalsoȱneedȱpermissionȱfromȱtheȱleaseholder.ȱItȱisȱbetterȱtoȱaccessȱ mangrovesȱthroughȱaȱboardwalk,ȱwhereȱtheseȱexist,ȱtoȱminimiseȱyourȱimpactȱonȱtheȱforest.ȱ What you may need: – GPSȱandȱtopographicȱmapȱorȱmapȱofȱtheȱboardwalkȱyouȱareȱaccessingȱ – 50Ȭmȱmeasuringȱtapeȱ – aȱmetalȱtapeȱȱ – leafȱtrapsȱ(500ȱmmȱ×ȱ500ȱmm)ȱtaggedȱwithȱuniqueȱnumbersȱȱ – heavyȬdutyȱnylonȱcord,ȱpreferablyȱinȱaȱbrightȱcolourȱȱ – aȱlargeȱplasticȱbagȱforȱeachȱtrapȱȱ – sixȱsmallerȱplasticȱbagsȱforȱeachȱtrapȱ – labȱscalesȱ(accurateȱtoȱ0.01ȱg)ȱȱ – accessȱtoȱdryingȱovensȱ – litterȱrecordȱform.ȱ 92 ESTUARINE MONITORING GUIDANCE MANUAL To ensure good results: Leafȱlitterȱvariesȱconsiderablyȱbetweenȱtrees,ȱdependingȱmainlyȱonȱtheȱsizeȱofȱtheȱtreesȱandȱtheirȱlocationȱ inȱrelationȱtoȱtheȱtide.ȱManyȱtransectsȱwouldȱbeȱrequiredȱtoȱallowȱaȱcomparisonȱofȱsites,ȱbutȱfewerȱ locationsȱareȱrequiredȱtoȱmonitorȱoneȱsiteȱoverȱtime.ȱEnsureȱyouȱselectȱaȱrangeȱofȱsimilarȱsizedȱtreesȱ throughȱtheȱwidthȱofȱtheȱforest,ȱandȱmarkȱthoseȱtreesȱsoȱthatȱyouȱmayȱuseȱthemȱagainȱinȱsubsequentȱ years.ȱ What to do: Selectȱaȱsurveyȱareaȱwithinȱtheȱtallestȱpartȱofȱtheȱforest.ȱTheȱstuntedȱlandwardȱedgesȱofȱtheȱforestȱwillȱnotȱ provideȱrepresentativeȱsamples.ȱDetermineȱtheȱapproximateȱcanopyȱheightȱusingȱaȱmetalȱtapeȱandȱ recordȱthisȱonȱyourȱlitterȱrecordȱform.ȱȱ Ifȱyouȱareȱusingȱaȱboardwalkȱtoȱaccessȱtheȱmangroveȱforest,ȱtrapsȱmayȱbeȱinstalledȱatȱregularȱintervalsȱ alongȱtheȱboardwalk,ȱthroughȱtheȱcentralȱportionȱofȱtheȱforest.ȱAlternatively,ȱsetȱyourȱtrapsȱsoȱthatȱeachȱ trapȱisȱaȱsimilarȱdistanceȱfromȱtheȱlowȱorȱhighȱtideȱlines.ȱTheȱnumberȱofȱtrapsȱusedȱisȱentirelyȱupȱtoȱtheȱ resourcesȱofȱtheȱgroup,ȱalthoughȱmoreȱthanȱoneȱisȱrequiredȱtoȱaccountȱforȱvariabilityȱbetweenȱplants.ȱ Eightȱorȱmoreȱprovideȱaȱreasonableȱnumberȱifȱtheȱgroupȱhasȱsufficientȱresources.ȱȱ Toȱinstallȱaȱtrap,ȱattachȱaȱnylonȱcordȱtoȱeachȱcornerȱofȱtheȱtrapȱandȱtieȱitȱontoȱtheȱmangroveȱbranchesȱsoȱ thatȱtheȱtrapȱhangsȱfairlyȱlevel.ȱMakeȱsureȱthatȱtheȱbottomȱofȱtheȱtrapȱisȱaboveȱtheȱhighȱtideȱmark,ȱwhichȱ isȱoftenȱvisibleȱinȱaȱmangroveȱforestȱasȱaȱlineȱofȱdebrisȱcaughtȱinȱtheȱbranches.ȱȱ Mangroveȱlitterȱtrapsȱneedȱtoȱbeȱemptiedȱfortnightly,ȱasȱAvicenniaȱmarinaȱleavesȱhaveȱsaltȱinȱthem,ȱandȱ thisȱattractsȱmoisture,ȱcausingȱthemȱtoȱdecomposeȱquickly.ȱEmptyȱtheȱtrapȱbyȱremovingȱanyȱlargeȱsticksȱ andȱplacingȱthemȱinȱplasticȱbagȱmarkedȱwithȱtheȱtrapȱnumber.ȱNextȱyouȱplaceȱtheȱbagȱunderȱtheȱtrapȱ chute,ȱuntieȱtheȱchuteȱandȱemptyȱtheȱtrapȱcontents.ȱRetieȱtheȱchuteȱsecurelyȱthenȱmoveȱonȱtoȱtheȱnextȱ trap.ȱ Shouldȱaȱlargeȱstormȱorȱkingȱtideȱdisruptȱyourȱmonitoringȱbyȱstrippingȱlargeȱamountsȱofȱleavesȱorȱfillingȱ theȱtrapȱwithȱdebris,ȱemptyȱtheȱtrapȱontoȱtheȱforestȱfloorȱandȱexamineȱitȱforȱdamage.ȱRecordȱthatȱtheȱ dataȱwasȱlostȱforȱthisȱtrapȱduringȱthisȱperiod.ȱResetȱtheȱtrapȱandȱcontinueȱmonitoring.ȱȱ Backȱatȱyourȱbase,ȱsortȱleafȱlitterȱcontentsȱfromȱeachȱtrapȱintoȱtheȱfollowingȱgroups:ȱleaves,ȱflowers,ȱbarkȱ (includeȱwood),ȱpropagulesȱandȱ‘other’ȱ(oftenȱdriedȱseagrassȱorȱalgaeȱthatȱmayȱhaveȱblownȱintoȱtheȱtrapȱ fromȱnearbyȱbranches).ȱDoȱnotȱmixȱtheȱcontentsȱofȱoneȱtrapȱwithȱmaterialȱfromȱotherȱtraps.ȱForȱeachȱ trap,ȱcountȱtheȱnumberȱofȱpartsȱinȱeachȱcategoryȱ(ifȱpossible)ȱandȱrecordȱtheseȱnumbersȱonȱtheȱdatasheet.ȱȱ Placeȱtheȱsortedȱcontentsȱintoȱlabelledȱsmallerȱbagsȱandȱplaceȱtheseȱbagsȱtogetherȱintoȱtheȱoriginalȱ labelledȱplasticȱbagȱforȱstorageȱuntilȱdrying.ȱIfȱthereȱisȱtooȱmuchȱlitterȱtoȱdryȱatȱonce,ȱorȱtheȱovensȱareȱnotȱ immediatelyȱavailable,ȱlitterȱmayȱbeȱrefrigeratedȱforȱupȱtoȱaȱweekȱbeforeȱbeingȱdried.ȱȱ Theȱlitterȱshouldȱbeȱdriedȱinȱitsȱseparateȱcategoriesȱandȱtrapȱnumbersȱatȱ70°Cȱforȱthreeȱfullȱdays.ȱTheȱ litterȱisȱdryȱwhenȱitȱisȱbrittleȱandȱtheȱleavesȱareȱeasilyȱcrumbledȱbetweenȱyourȱfingers.ȱIfȱtheȱweatherȱisȱ hotȱandȱdryȱitȱmayȱbeȱpossibleȱtoȱdry,ȱorȱpartiallyȱdry,ȱtheȱlitterȱinȱaȱshelteredȱplaceȱinȱtheȱsun,ȱunderȱ glassȱorȱbehindȱaȱwindow.ȱKeepȱaȱcarefulȱeyeȱonȱtheȱlitterȱtoȱensureȱitȱdoesȱnotȱburn.ȱTheȱpropagulesȱ manyȱnotȱdryȱcompletely,ȱsoȱcountsȱareȱimportantȱtoȱsupplementȱtheȱweights.ȱȱ Onceȱtheȱlitterȱisȱdry,ȱuseȱlabȱscalesȱtoȱseparatelyȱweighȱtheȱcontentsȱfromȱeachȱcategoryȱandȱeachȱtrap.ȱ Recordȱeachȱofȱtheȱweightsȱ(inȱgramsȱtoȱtwoȱdecimalȱplaces)ȱonȱtheȱdatasheet.ȱDisposeȱofȱlitterȱbackȱtoȱ whereȱitȱwasȱcollectedȱorȱintoȱmunicipalȱwaste.ȱȱ Theȱdataȱfromȱthisȱactivityȱisȱexpressedȱasȱg/m2/fortnight,ȱforȱeachȱevent.ȱIfȱyouȱwishȱtoȱlookȱatȱ productionȱannually,ȱexpressȱtheȱdataȱasȱaȱrangeȱofȱvaluesȱperȱmonth,ȱegȱx–yȱg/m2/month,ȱorȱpresentȱtheȱ dataȱasȱaȱgraphȱshowingȱeachȱsamplingȱevent.ȱȱ Significantȱdeclinesȱorȱsuddenȱincreasesȱinȱleafȱlitterȱfallȱorȱreproductiveȱeffortȱmayȱindicateȱthatȱtheȱ communityȱisȱunderȱstress.ȱSeekȱadviceȱifȱsuchȱtrendsȱoccur.ȱȱ 93 ESTUARINE MONITORING GUIDANCE MANUAL Monitoring mangrove expansion Wetȱcoastalȱecosystemsȱcontainingȱhabitatsȱsuchȱasȱmangrovesȱandȱ saltmarshesȱareȱseralȱcommunitiesȱ(Chapman,ȱ1960).ȱTheȱ distributionȱofȱtheȱdifferentȱhabitatsȱisȱgovernedȱbyȱsedimentȱ supply,ȱtidalȱregime,ȱwindȬwaveȱclimateȱandȱtheȱrelativeȱmovementȱ ofȱlocalȱseaȱlevelȱ(PackhamȱandȱWillis,ȱ1997).ȱȱ Forȱtheȱdevelopmentȱofȱmangalsȱandȱsaltmarshes,ȱsandȱorȱmudȱflatsȱ mustȱaccumulateȱuntilȱtheirȱlevelȱisȱhighȱenoughȱtoȱallowȱ colonisationȱbyȱplants.ȱThisȱcanȱonlyȱoccurȱwhereȱthereȱisȱsufficientȱ sedimentȱsupplyȱ(eitherȱfromȱlandȱorȱoffshore)ȱandȱwhereȱtheȱwaterȱ flowsȱareȱofȱlowȱenergy.ȱConstructionȱofȱembankments,ȱdeathȱofȱ offshoreȱbenthicȱfloraȱsuchȱasȱseagrassȱbedsȱandȱchannellingȱofȱ stormwaterȱflowsȱmayȱinfluenceȱbankȱformationȱbyȱchangingȱtheȱ tidalȱregime,ȱwaterȱflowȱratesȱorȱwaveȱaction.ȱ Changesȱinȱtheseȱcommunitiesȱoccurȱreasonablyȱquickly,ȱandȱ simpleȱphotographicȱmonitoringȱmayȱdetectȱtheȱchangesȱwithinȱaȱ fewȱyears.ȱOneȱparticularlyȱusefulȱwayȱofȱassessingȱthisȱchangeȱisȱtoȱ establishȱaȱphotopointȱatȱaȱboatȱrampȱthatȱtraversesȱtheȱwidthȱofȱaȱ mangroveȱforest.ȱ Activity: 3 Extension Age: High school Time: 2 Why? Regular examination of the seaward and landward edges of a mangrove forest can determine whether the forest is expanding or retreating landward. This may indicate local sea-level change, or sediment availability. What you may need: – cameraȱ – fixedȱlocationsȱforȱtakingȱphotographsȱ(mayȱbeȱpaintȱmarksȱonȱaȱboatȱramp,ȱaboveȱwhichȱyouȱ standȱyourȱcameraȱtripod,ȱaȱmarkedȱjettyȱrailȱorȱsomethingȱsimilar)ȱ – aȱmetreȱruleȱorȱotherȱmeasuringȱstickȱwithȱtheȱdateȱmarkedȱonȱaȱsheetȱattachedȱtoȱit.ȱ To ensure good results: Chooseȱaȱramp,ȱwharfȱorȱotherȱlocationȱwhereȱyouȱcanȱseeȱalongȱbothȱtheȱseawardȱandȱlandwardȱedgesȱ ofȱaȱmangroveȱforest.ȱEnsureȱthatȱyouȱcanȱaccessȱbothȱareasȱtoȱplaceȱyourȱmetreȱruleȱagainstȱaȱtree.ȱ What to do: Thisȱactivityȱisȱbestȱconductedȱannually,ȱtoȱrecordȱtheȱexpansionȱorȱretreatȱofȱtheȱedgesȱofȱtheȱmangroveȱ forest.ȱȱ Establishȱaȱlocationȱthatȱisȱeasyȱtoȱmarkȱandȱcloseȱtoȱtheȱseawardȱedgeȱofȱtheȱmangrovesȱfromȱwhichȱtoȱ takeȱyourȱphotographs.ȱPlaceȱyourȱmetreȱruleȱagainstȱaȱmangroveȱtreeȱthatȱwillȱbeȱinȱtheȱforegroundȱofȱ theȱphotograph.ȱTakeȱaȱshotȱthatȱlooksȱalongȱtheȱseawardȱedgeȱandȱthatȱincludesȱyourȱmetreȱruleȱasȱaȱ scaleȱandȱdate.ȱȱ Moveȱinlandȱuntilȱyouȱreachȱaȱpointȱwhereȱyouȱcanȱseeȱalongȱtheȱlandwardȱedgeȱofȱtheȱforest.ȱRepeatȱ theȱactivityȱoutlinedȱabove.ȱ Onceȱyouȱhaveȱcollectedȱseveralȱyearsȱofȱphotographsȱyouȱmayȱwishȱtoȱscanȱtheȱprints,ȱandȱenlargeȱorȱ reduceȱthemȱsoȱthatȱtheȱmetreȱruleȱisȱtheȱsameȱsizeȱinȱallȱofȱthem.ȱMostȱimageȱeditingȱsoftwareȱwillȱallowȱ youȱdoȱthis,ȱandȱtoȱoverlayȱtheȱphotographsȱsoȱthatȱyouȱcanȱseeȱtheȱdifference.ȱAlternatively,ȱyouȱcouldȱ scaleȱtheȱprintsȱusingȱaȱphotocopier,ȱthenȱplaceȱaȱgridȱoverȱtheȱscaledȱphotographsȱtoȱestimateȱtheȱ change,ȱasȱdescribedȱinȱtheȱguidelineȱUsingȱaerialȱphotographsȱtoȱmapȱhabitatȱchange.ȱ Isȱyourȱmangroveȱforestȱexpanding,ȱretreatingȱorȱmovingȱinland?ȱ 94 ESTUARINE MONITORING GUIDANCE MANUAL Studying saltmarsh plants SaltmarshesȱinȱSouthȱAustraliaȱhaveȱchangedȱextensivelyȱsinceȱ Europeanȱsettlement.ȱDrainsȱandȱdykesȱhaveȱbeenȱusedȱtoȱconvertȱ marshȱareasȱintoȱagriculturalȱlands,ȱandȱembankmentsȱhaveȱchangedȱ theȱhydrologyȱsignificantly.ȱȱ Thereȱisȱevidenceȱtoȱsuggestȱthat,ȱinȱtheȱPortȱAdelaideȱarea,ȱ saltmarshȱcommunitiesȱareȱdecliningȱdueȱtoȱaȱlandwardȱexpansionȱofȱ mangroveȱcommunities.ȱThisȱisȱdueȱtoȱaȱcombinationȱofȱsoilȱ subsidenceȱ(aȱresultȱofȱembankmentȱworksȱinȱtheȱ1890s)ȱandȱseaȱlevelȱ rise.ȱȱ Healthyȱsaltmarshȱcommunitiesȱshowȱaȱzonationȱfromȱtheȱlowerȱ marshȱtoȱtheȱupperȱmarsh.ȱInȱSouthȱAustralia,ȱsaltmarshesȱareȱ samphireȬdominated.ȱSpeciesȱinȱtheȱlowerȱmarshȱmayȱincludeȱ Sarcocornia,ȱthenȱthereȱareȱbandsȱofȱSclerostegia,ȱHalosarcia,ȱMaireanaȱ andȱAtriplex,ȱandȱthenȱatȱtheȱhighestȱzoneȱaȱbandȱofȱgrassesȱmayȱbeȱ found.ȱ Thisȱactivityȱlooksȱatȱtheȱpercentȱcoverȱandȱspeciesȱdominanceȱofȱ saltmarshȱspeciesȱalongȱaȱlineȱintersectȱtransect.ȱTheȱtransectȱcanȱrunȱ eitherȱperpendicularȱtoȱ(fromȱlandȱtoȱtheȱmangroveȱfringe),ȱorȱ parallelȱto,ȱtheȱtidalȱgradient.ȱSamphiresȱmayȱbeȱveryȱdenseȱandȱ growȱoverȱhighlyȱunevenȱterrain,ȱandȱsoȱitȱisȱpreferableȱtoȱworkȱfromȱ aȱboardwalk.ȱIfȱyouȱhaveȱaccessȱtoȱaȱboardwalk,ȱyouȱmayȱconsiderȱitȱ toȱbeȱtheȱtransectȱlineȱandȱassessȱtheȱplantsȱtoȱoneȱsideȱofȱtheȱwalk.ȱȱ Theȱpresenceȱandȱdistributionȱofȱspeciesȱoverȱtimeȱisȱaȱsimpleȱbutȱ usefulȱindicatorȱofȱecosystemȱfunctionȱoverȱtime,ȱwithȱtheirȱlossȱorȱ declineȱindicatingȱpotentialȱenvironmentalȱstressȱ(Zedler,ȱ2001).ȱ Vegetationȱpatternsȱmayȱchangeȱinȱresponseȱto,ȱandȱmayȱindicate,ȱ changingȱsoilȱsalinitiesȱorȱpH,ȱtidalȱflushingȱregimesȱorȱclimaticȱ patterns.ȱThereforeȱthisȱactivityȱisȱaȱusefulȱoneȱforȱassessingȱtheȱ progressȱofȱsaltmarshȱrehabilitationȱprojectsȱthatȱinvolveȱchangedȱ tidalȱregimesȱ(ieȱafterȱfloodgatesȱhaveȱbeenȱremovedȱorȱmodified).ȱȱ Activity: 3 Extension Age: High school Time: 3 Why? Saltmarsh areas are valuable wader habitat and fish nurseries. Saltmarshes are very vulnerable to hydrological change, which may be revealed by a change in the vegetation. Safety: Saltmarshes are home to snakes and other small creatures. They also have large numbers of hidden creeks or overhangs which are easy to fall down. Look where you are walking and ensure that all members of the group are wearing long pants and covered shoes. Saltmarsh environments have no shade and can become extremely hot. Wear a hat, take a bottle of water and try to work in the cooler parts of the day. What you may need: – 50–100ȱmȱtapeȱmeasureȱȱ – saltmarshȱidentificationȱguidesȱ – compassȱandȱGPSȱ – topographicȱorȱsiteȱmapȱ – plasticȱorȱpaintedȱwoodenȱstakesȱȱ – malletȱorȱhammerȱ – saltmarshȱvegetationȱrecordȱform.ȱ To ensure good results: Doȱnotȱmonitorȱimmediatelyȱafterȱaȱveryȱhighȱstormȱtide.ȱTheȱsaltmarshesȱareȱlikelyȱtoȱbeȱflattened,ȱ whichȱwillȱbiasȱresults.ȱ 95 ESTUARINE MONITORING GUIDANCE MANUAL What to do: S it eȱ selection ȱ Closelyȱexamineȱaerialȱphotographsȱofȱtheȱmarshȱyouȱwishȱtoȱmonitor.ȱIfȱthereȱisȱaȱboardwalkȱorȱ walkingȱpathȱthroughȱtheȱmarsh,ȱtakeȱaȱwalkȱalongȱit.ȱObserveȱtheȱspeciesȱcomposition,ȱcoverȱandȱ canopyȱheightȱofȱtheȱsaltmarshȱandȱtheȱextentȱofȱeachȱofȱtheȱhabitats.ȱȱ Itȱisȱimportantȱtoȱselectȱaȱmonitoringȱsiteȱthatȱisȱeasyȱtoȱaccess.ȱWalkingȱacrossȱsaltmarshesȱdamagesȱ them,ȱandȱtheyȱmayȱtakeȱaȱlongȱtimeȱtoȱrecover.ȱTheȱharderȱtheȱsiteȱisȱtoȱaccess,ȱtheȱmoreȱdamageȱ accessingȱitȱwillȱdo.ȱUsingȱtheȱedgeȱofȱaȱboardwalkȱorȱwalkingȱpathȱasȱtheȱtransectȱlineȱisȱprobablyȱbestȱ forȱminimisingȱdamageȱandȱmakingȱthisȱactivityȱeasierȱonȱparticipants.ȱȱ Theȱnextȱmostȱimportantȱpointȱisȱtoȱensureȱthatȱtheȱsiteȱappearsȱtoȱbeȱrepresentativeȱofȱtheȱstateȱofȱ saltmarshesȱinȱtheȱarea.ȱ E st a b l ish ingȱ aȱt r an sec tȱ or ȱ l in eȱ o fȱ in te rc ept ȱ Theȱdirectionȱandȱlengthȱofȱtheȱlineȱintersectȱtransectȱdependsȱonȱtheȱobjectivesȱofȱtheȱmonitoringȱ program,ȱsiteȱcharacteristicsȱandȱaccess.ȱȱ Intersectȱlinesȱthatȱrunȱfromȱtheȱmangroveȱedgeȱtoȱtheȱhighestȱpointȱinȱtheȱmarshȱwillȱgenerallyȱcaptureȱ theȱfullestȱrangeȱofȱhabitatȱgradients.ȱHowever,ȱlinesȱrunningȱparallelȱtoȱtheȱtideȱwillȱgiveȱyouȱtheȱlargestȱ sampleȱofȱoneȱhabitatȱtype.ȱȱ Eachȱtransectȱshouldȱbeȱ50ȱtoȱ100ȱmȱlong.ȱIfȱyouȱareȱrunningȱtheȱtransectȱlineȱacrossȱtheȱmarsh,ȱmeasureȱ thisȱoutȱandȱlayȱtheȱmeasuringȱtapeȱalongȱtheȱground.ȱAtȱtheȱstartȱpointȱofȱtheȱline,ȱhammerȱaȱsmallȱ stakeȱinȱ(withȱtheȱpermissionȱofȱtheȱlandholder).ȱRecordȱtheȱlocationȱusingȱaȱGPSȱorȱyourȱsiteȱmap.ȱ Hammerȱanotherȱstakeȱinȱatȱtheȱotherȱendȱandȱrecordȱitsȱlocation.ȱAttachȱtheȱmeasuringȱtapeȱtoȱeachȱ stakeȱsoȱthatȱitȱisȱrelativelyȱstraightȱandȱlevel.ȱ Ifȱyouȱareȱusingȱaȱboardwalkȱasȱaȱtransect,ȱsimplyȱmarkȱtheȱstartȱandȱendȱpointsȱwithȱaȱdabȱofȱpaintȱonȱ theȱboards,ȱandȱrecordȱtheirȱlocationsȱusingȱyourȱGPSȱorȱmap.ȱ Itȱisȱrecommendedȱthatȱ100–300ȱmȱofȱtransectȱisȱestablishedȱinȱeachȱareaȱofȱsaltmarshȱthatȱyouȱwishȱtoȱ monitor,ȱalthoughȱthisȱwillȱdependȱonȱyourȱresourcesȱandȱtimeȱavailability.ȱ M on it or i ngȱth eȱm ar sh ȱ Startingȱatȱtheȱbeginningȱofȱyourȱtransectȱandȱlookȱatȱtheȱplantsȱthatȱintersectȱit.ȱWhatȱisȱgrowingȱ directlyȱbelowȱtheȱmeasuringȱtape,ȱorȱrightȱnextȱtoȱtheȱboardwalk?ȱUseȱyourȱguidesȱtoȱidentifyȱitȱtoȱ eitherȱgenusȱorȱspeciesȱlevel.ȱIfȱyouȱcan’tȱidentifyȱit,ȱtakeȱaȱpictureȱorȱdescribeȱit.ȱAȱfewȱgoodȱ identificationȱbooksȱareȱlistedȱatȱtheȱendȱofȱthisȱguideline.ȱHowȱmuchȱofȱtheȱfirstȱmetreȱofȱtransectȱlineȱ doesȱeachȱspeciesȱcover?ȱȱ Recordȱtheȱdistanceȱalongȱyourȱtransectȱthisȱspeciesȱcovers.ȱForȱexample,ȱyouȱmayȱhaveȱaȱtwoȱmetreȬ wideȱswatheȱofȱSarcocorniaȱquinqueflora.ȱItȱisȱnotȱnecessaryȱtoȱrecordȱbareȱmudȱifȱtheȱpatchesȱareȱlessȱthanȱ 200ȱmmȱacross.ȱBareȱareasȱlargerȱthanȱthisȱneedȱtoȱbeȱrecorded.ȱȱ Lookȱatȱeachȱmetreȱalongȱtheȱtransectȱlineȱandȱmakeȱaȱsimilarȱassessment.ȱIfȱyouȱgetȱlongȱlengthsȱofȱtheȱ sameȱthing,ȱjustȱrecordȱthisȱasȱoneȱentryȱandȱdrawȱbracketsȱenclosingȱtheȱareaȱitȱcovers.ȱ Whenȱyouȱgetȱtoȱtheȱendȱofȱtheȱfirstȱtransectȱworkȱoutȱtheȱtotalȱamountȱofȱeachȱspeciesȱinȱlinearȱmetresȱ orȱpercentage.ȱRecordȱtheȱunitȱthatȱyouȱuse.ȱRepeatȱthisȱprocedureȱforȱeachȱofȱyourȱtransects.ȱȱ Theȱinformationȱyouȱobtainȱwillȱtellȱyouȱaboutȱspeciesȱdominance,ȱtheȱpercentageȱcoverȱofȱsaltmarshȱ speciesȱandȱtheȱpercentageȱcoverȱofȱweedȱspecies.ȱAȱcopyȱofȱyourȱfieldȱsheetȱandȱcalculationsȱforȱtheȱ figuresȱmentionedȱaboveȱshouldȱbeȱsentȱtoȱyourȱWaterwatchȱcoordinator.ȱȱ Thisȱactivityȱdetectsȱchangesȱthatȱoccurȱoverȱaȱlongȱperiodȱandȱthatȱmayȱnotȱbeȱnoticeableȱtoȱtheȱeye.ȱAnȱ annualȱmeasurementȱatȱtheȱsameȱseasonȱmayȱbeȱrequiredȱforȱthreeȱyearsȱorȱmore,ȱbeforeȱyouȱcanȱdetectȱ slowȱdegradation.ȱTwoȱmeasurementsȱaȱyearȱapartȱmayȱpickȱupȱanyȱrapidȱimprovementȱthatȱoccursȱonȱ remediatedȱsites.ȱAȱonceȬonlyȱmeasurementȱwillȱtellȱyouȱquiteȱaȱlotȱaboutȱtheȱtypeȱofȱsaltmarshȱyouȱareȱ 96 ESTUARINE MONITORING GUIDANCE MANUAL monitoring,ȱbutȱwillȱnotȱtellȱyouȱmuchȱaboutȱanyȱenvironmentalȱimpactsȱthatȱmayȱbeȱoccurring,ȱapartȱ fromȱtheȱpresenceȱandȱquantityȱofȱweeds.ȱȱ Someȱareas,ȱsuchȱasȱtheȱhighȱmarshȱareasȱnearȱPortȱPirieȱthatȱabutȱnaturalȱsabkhasȱ(giantȱmudȱorȱsaltȱ flats),ȱwillȱnaturallyȱhaveȱaȱlowȱpercentageȱcover,ȱandȱwillȱbeȱdominatedȱbyȱaȱveryȱfewȱspecies.ȱOtherȱ areas,ȱparticularlyȱlowȱmarshes,ȱwillȱhaveȱaȱhighȱpercentageȱcoverȱandȱwillȱalsoȱhaveȱhighȱnumberȱofȱ species.ȱNeitherȱofȱtheseȱareȱmore,ȱorȱless,ȱhealthyȱthanȱtheȱother.ȱȱ Aȱusefulȱpurposeȱofȱsaltmarshȱmonitoringȱisȱtoȱperformȱaȱ‘beforeȱandȱafter’ȱassessmentȱforȱregenerationȱ projectsȱorȱhydrologicalȱchangesȱonȱtheȱsite.ȱIfȱtheȱmonitoringȱisȱbeingȱusedȱforȱthisȱpurpose,ȱcontrolȱ sitesȱshouldȱbeȱestablishedȱtoȱdetermineȱifȱtheȱdetectedȱchangesȱareȱsiteȱspecificȱorȱareȱtheȱresultȱofȱmoreȱ widespreadȱvariationsȱsuchȱasȱtheȱweather.ȱȱ Dataȱonȱsoilȱsalinity,ȱpH,ȱsoilȱelevationȱandȱtidalȱflushingȱregimesȱwouldȱalsoȱbeȱusefulȱwhenȱ interpretingȱtheȱsaltmarshȱspeciesȱdata.ȱGuidelinesȱonȱmeasuringȱtheseȱparametersȱareȱavailableȱinȱ earlierȱsectionsȱofȱthisȱmanual.ȱȱ U s e f u l r e f e r e n ce s Dashorst,ȱGȱ&ȱJessop,ȱJȱ(1998),ȱPlantsȱofȱtheȱAdelaideȱPlainsȱ&ȱHills,ȱ2ndȱedition,ȱSouthȱAustralianȱStateȱ Herbarium,ȱFinsburyȱPress.ȱȱ Datson,ȱBȱ(2002),ȱSamphiresȱinȱWesternȱAustralia:ȱAȱFieldȱGuideȱtoȱChenopodiaceaeȱTribeȱSalicorniae,ȱ DepartmentȱofȱConservationȱandȱLandȱManagement,ȱPerth.ȱȱ George,ȱAȱ(ed)(1984),ȱFloraȱofȱAustralia:ȱVolumeȱ4—PhytolaccaceaeȱtoȱChenpodiaceae,ȱCommonwealthȱofȱ Australia,ȱGriffinȱPress,ȱSouthȱAustralia.ȱȱ Jessop,ȱJȱ&ȱToelken,ȱHȱ(1986),ȱFloraȱofȱSouthȱAustralia.ȱPartsȱI—IV,ȱ4thȱedition,ȱSouthȱAustralianȱStateȱ Herbarium,ȱSouthȱAustralianȱGovernmentȱPress,ȱAdelaide.ȱȱ Zedler,ȱJBȱ(2001),ȱHandbookȱforȱrestoringȱtidalȱwetlands,ȱCRCȱPress,ȱBocaȱRaton,ȱFlorida.ȱ ȱ ȱ 97 ESTUARINE MONITORING GUIDANCE MANUAL Monitoring Melaleuca leaf/twig litter production InȱwetterȱareasȱofȱSouthȱAustralia,ȱMelaleucaȱorȱTeaȬTreeȱswampsȱ oftenȱreplaceȱmangrovesȱatȱtheȱfresherȱendȱofȱestuaries.ȱȱ ‘Litterȱproduction’ȱmeansȱtheȱsheddingȱofȱleaves,ȱtwigs,ȱflowers,ȱ seedsȱorȱotherȱvegetativeȱmatterȱbyȱaȱplant.ȱThisȱmayȱbeȱcausedȱbyȱ age,ȱstressȱorȱmechanicalȱfactorsȱsuchȱasȱwindȱorȱfloods.ȱȱ TheȱhealthȱofȱaȱMelaleucaȱswampȱmayȱbeȱassessedȱbyȱmeasuringȱtheȱ litterȱproductionȱofȱitsȱmajorȱplantȱspecies.ȱAȱhealthyȱsystemȱwillȱ haveȱaȱhighȱand/orȱstableȱmonthlyȱleafȱlitterȱasȱtheȱolderȱleavesȱareȱ shedȱwhenȱtheȱplantȱproducesȱnewerȱones,ȱalthoughȱthereȱmayȱbeȱ largeȱseasonalȱvariations.ȱDecliningȱleafȱlitterȱproductionȱoverȱseveralȱ yearsȱmayȱindicateȱthatȱtheȱcommunityȱisȱunderȱstress.ȱTheȱquantityȱ ofȱreproductiveȱmaterialȱmayȱalsoȱbeȱusedȱasȱaȱmeasureȱofȱhealthȱasȱ reproductionȱeffortȱchangesȱifȱplantsȱareȱstressed.ȱȱ LitterȱfallȱfromȱMelaleucaȱspeciesȱrepresentsȱaȱsignificantȱpartȱofȱtheȱ netȱprimaryȱproductivityȱofȱaȱsupratidalȱMelaleucaȱswamp.ȱExamplesȱ ofȱtheseȱtypesȱofȱswampsȱcanȱbeȱfoundȱinȱestuariesȱthroughoutȱtheȱ southeastȱofȱSouthȱAustralia.ȱȱ Litterȱfallȱisȱmeasuredȱbyȱinstallingȱleafȱlitterȱtrapsȱalongȱaȱtransectȱinȱ aȱMelaleucaȱcommunity.ȱTrappedȱlitterȱisȱcollectedȱandȱsortedȱintoȱ differentȱcategories,ȱovenȱdriedȱandȱthenȱweighed.ȱTheȱdryȱweightȱofȱ eachȱcategoryȱprovidesȱaȱmeasureȱofȱtheȱlitterȱproductivityȱofȱtheȱ Melaleucaȱcommunity.ȱThisȱmethodȱshouldȱonlyȱbeȱattemptedȱifȱ groupsȱcanȱobtainȱaccessȱtoȱdryingȱovens.ȱȱ Activity: 3 Extension Age: High school Time: 3 Why? In SA, Melaleuca halmaturorum fringing wetlands are often present in the estuarine parts of swampy watercourses. These freshwater-intertidal wetlands are an important filter system for floodwaters, and also attenuate the progression of floods into the more marine parts of the estuary. Safety: Snakes may pose danger during the summer months, especially in long grass. Always wear suitable clothing (strong shoes and long trousers) and work in pairs to minimise the risk. Aȱpermitȱmayȱbeȱrequiredȱforȱthisȱactivity.ȱAttemptȱtoȱfindȱoutȱwhoȱ ownsȱtheȱlandȱyouȱplanȱtoȱcollectȱlitterȱfrom.ȱIfȱprivatelyȱowned,ȱgetȱ writtenȱpermissionȱfromȱtheȱowner.ȱIfȱtheȱlandȱisȱownedȱbyȱtheȱ Crown,ȱaȱpermitȱtoȱtakeȱnativeȱvegetationȱ(availableȱfromȱtheȱNPWS)ȱ mayȱbeȱrequired.ȱȱ Keep a careful eye on the litter when drying it to ensure it does not catch alight, and ensure no other flammables are near the litter. What you may need: Heat risks apply when working with ovens. – GPSȱandȱtopographicȱmapȱ – 50Ȭmȱmeasuringȱtapeȱ – steelȱmeasuringȱtapeȱ – leafȱtrapsȱ(500ȱ×ȱ500ȱmm)ȱtaggedȱuniqueȱnumbersȱȱ – fourȱwoodenȱgardenȱstakesȱforȱeachȱtrapȱ – heavyȬdutyȱnylonȱcord,ȱpreferablyȱinȱaȱbrightȱcolourȱȱ – aȱlargeȱplasticȱbagȱforȱeachȱtrapȱȱ – sixȱsmallerȱplasticȱbagsȱforȱeachȱtrapȱ – labȱscalesȱ(accurateȱtoȱ0.01ȱg)ȱȱ – accessȱtoȱdryingȱovensȱ – litterȱrecordȱform.ȱ To ensure good results: Selectȱyourȱsurveyȱsiteȱsoȱthatȱitȱisȱcompletelyȱcontainedȱinȱhabitatȱthatȱappearsȱhomogenous.ȱIfȱtheȱ habitatȱchanges,ȱthenȱlitterȱcollectionȱwillȱalsoȱvary.ȱ 98 ESTUARINE MONITORING GUIDANCE MANUAL What to do: Recordȱtheȱdominantȱspeciesȱpresent,ȱandȱuseȱtheȱsteelȱtapeȱtoȱapproximateȱtheȱcanopyȱheightȱofȱyourȱ site.ȱRecordȱthisȱonȱyourȱlitterȱrecordȱform.ȱȱ Trapsȱmayȱeitherȱbeȱinstalledȱatȱregularȱintervalsȱalongȱaȱtransectȱorȱlocatedȱrandomlyȱinȱaȱ20ȱmȱ×ȱ20ȱmȱ grid.ȱTheȱnumberȱofȱtrapsȱusedȱisȱentirelyȱupȱtoȱtheȱresourcesȱofȱtheȱgroup.ȱHowever,ȱmoreȱthanȱoneȱisȱ requiredȱtoȱaccountȱforȱvariabilityȱbetweenȱplants.ȱEightȱorȱmoreȱisȱaȱgoodȱnumberȱifȱtheȱgroupȱhasȱ sufficientȱresources.ȱȱ Toȱinstallȱeachȱtrap,ȱhammerȱfourȱstakesȱintoȱtheȱgroundȱuntilȱaboutȱoneȱmetreȱhigh.ȱPlaceȱthemȱinȱaȱ squareȱpatternȱjustȱoverȱ500Ȭmmȱapart.ȱAttachȱtheȱtrapȱtoȱtheȱstakesȱwithȱheavyȬdutyȱcord,ȱensuringȱthatȱ theȱchuteȱisȱoffȱtheȱground.ȱTheȱtrapȱisȱnotȱgenerallyȱsecuredȱtoȱtheȱtree,ȱasȱMelaleucaȱbranchesȱsnapȱ easily.ȱEnsureȱthatȱtheȱchutesȱareȱsecurelyȱtied.ȱȱ IfȱyourȱareaȱofȱMelaleucaȱswampȱisȱregularlyȱinundated,ȱlocateȱtheȱtrapsȱinȱtheȱhigherȱareasȱofȱtheȱ wetland,ȱdiscontinueȱmonitoringȱduringȱfloodȱperiodsȱorȱplaceȱtheȱtrapsȱhighȱenoughȱaboveȱtheȱgroundȱ toȱpreventȱinundation.ȱȱ Ifȱtrapsȱbecomeȱfloodedȱduringȱwetȱperiodsȱdiscontinueȱleafȱcollectingȱfromȱthisȱtrapȱuntilȱtheȱfloodȱhasȱ dispersed.ȱWhenȱtheȱwaterȱisȱgone,ȱreturnȱtoȱtrapȱandȱemptyȱitȱontoȱtheȱground.ȱThisȱlitterȱwillȱhaveȱ rottedȱorȱhaveȱbeenȱcontaminatedȱwithȱsediment,ȱsoȱisȱnotȱuseful.ȱRecordȱthatȱtheȱdataȱwasȱlostȱforȱthisȱ period.ȱResetȱtheȱtrapȱtoȱcontinueȱmonitoring.ȱȱ Trapsȱneedȱtoȱbeȱemptiedȱeveryȱmonth.ȱThisȱisȱtoȱpreventȱleafȱdecayȱandȱtoȱhelpȱdetermineȱmonthlyȱorȱ seasonalȱtrends.ȱEmptyȱtheȱtrapȱbyȱremovingȱanyȱlargeȱsticksȱandȱthenȱplaceȱthemȱinȱaȱplasticȱbagȱ markedȱwithȱtheȱtrapȱnumber.ȱNextȱyouȱplaceȱtheȱbagȱunderȱtheȱchute,ȱuntieȱtheȱchuteȱandȱemptyȱtheȱ trapȱcontents.ȱRetieȱtheȱchuteȱsecurelyȱthenȱmoveȱonȱtoȱtheȱnextȱtrap.ȱȱ Onceȱbackȱatȱyourȱbase,ȱsortȱleafȱlitterȱcontentsȱfromȱeachȱtrapȱintoȱtheȱfollowingȱgroups:ȱleaves,ȱflowers,ȱ barkȱ(includeȱwood),ȱseeds,ȱotherȱspeciesȱ(nonȱMelaleuca)ȱandȱweeds.ȱDoȱnotȱmixȱcontentsȱofȱoneȱtrapȱ withȱmaterialȱfromȱotherȱtraps.ȱCountȱtheȱnumberȱofȱpartsȱinȱeachȱcategoryȱ(ifȱpossible)ȱandȱrecordȱtheseȱ numbersȱonȱtheȱdatasheet.ȱȱ Placeȱtheȱsortedȱcontentsȱintoȱsmallerȱbagsȱandȱtheȱsmallerȱbagsȱbackȱintoȱtheȱoriginalȱlabelledȱplasticȱ bagȱforȱstorageȱuntilȱdryingȱcanȱbeȱattempted.ȱIfȱthereȱisȱtooȱmuchȱlitterȱtoȱdryȱatȱonce,ȱorȱtheȱovensȱareȱ notȱimmediatelyȱavailable,ȱlitterȱcontentsȱmayȱbeȱrefrigeratedȱforȱupȱtoȱaȱweek.ȱȱ Theȱlitterȱshouldȱbeȱdriedȱinȱtheirȱseparateȱcategoriesȱandȱtrapȱnumbersȱatȱ70°Cȱforȱthreeȱfullȱdays.ȱTheȱ litterȱisȱdryȱwhenȱitȱisȱbrittleȱandȱtheȱleavesȱareȱeasilyȱcrumbledȱbetweenȱyourȱfingers.ȱIfȱtheȱweatherȱisȱ hotȱandȱdryȱitȱmayȱbeȱpossibleȱtoȱdry,ȱorȱpartiallyȱdry,ȱtheȱlitterȱinȱaȱshelteredȱplaceȱinȱtheȱsun,ȱunderȱ glassȱorȱbehindȱaȱwindow.ȱKeepȱaȱcarefulȱeyeȱonȱtheȱlitterȱtoȱensureȱitȱdoesȱnotȱburn.ȱȱ Onceȱtheȱlitterȱisȱdry,ȱuseȱlabȱscalesȱtoȱseparatelyȱweighȱtheȱcontentsȱfromȱeachȱcategoryȱandȱeachȱtrap.ȱ Recordȱeachȱofȱtheȱweightsȱ(inȱgramsȱtoȱtwoȱdecimalȱplaces)ȱontoȱtheȱdatasheet.ȱDisposeȱofȱleafȱlitterȱ backȱtoȱwhereȱitȱwasȱcollectedȱorȱintoȱmunicipalȱwaste.ȱȱ Theȱdataȱforȱeachȱsamplingȱeventȱisȱexpressedȱasȱg/m2/month.ȱShouldȱyouȱwishȱtoȱlookȱatȱanȱannualȱ figure,ȱexpressȱtheȱdataȱasȱrangesȱofȱvaluesȱperȱmonth,ȱegȱx–yȱg/m2/month,ȱorȱgraphȱtheȱyear’sȱevents.ȱȱ Significantȱdeclinesȱinȱleafȱlitterȱfallȱorȱreproductiveȱeffortȱmayȱindicateȱthatȱtheȱcommunityȱisȱunderȱ stress.ȱSeekȱadviceȱifȱsuchȱtrendsȱoccur.ȱȱ 99 ESTUARINE MONITORING GUIDANCE MANUAL Using aerial photographs to map habitat change Theȱfirstȱtimeȱaeroplanesȱwereȱusedȱtoȱphotographȱaȱlargeȱsectionȱofȱ SouthȱAustraliaȱwasȱinȱ1949.ȱThisȱareaȱincludedȱAdelaideȱandȱitsȱ surroundingȱplains.ȱTheȱphotosȱwereȱinȱblackȱandȱwhite,ȱbutȱtheȱ qualityȱwasȱexceptionalȱforȱtheȱtime.ȱ Inȱtheȱlastȱ54ȱyearsȱtheseȱaerialȱphotographsȱareȱtakenȱonȱaȱregularȱ basis.ȱTheȱAdelaideȱmetropolitanȱareaȱisȱphotographedȱatȱleastȱ everyȱyear.ȱOtherȱlowlyȱpopulatedȱareasȱareȱphotographedȱlessȱ frequently.ȱȱ Theseȱphotographsȱwereȱinitiallyȱtakenȱforȱplanningȱreasons,ȱasȱ theyȱareȱusedȱtoȱcreateȱupȬtoȬdateȱorthophotoȱmapsȱinȱtheȱ1:20,000,ȱ 1:10,000,ȱ1:5000ȱandȱ1:2500ȱscales.ȱEnvironmentalȱscientists,ȱ managersȱandȱgeologistsȱquicklyȱfoundȱusesȱforȱaerialȱphotographs,ȱ asȱtheyȱareȱplentifulȱsourcesȱofȱdata.ȱAerialȱphotographyȱisȱnowȱ commonlyȱavailableȱinȱblackȱandȱwhite,ȱcolour,ȱfalseȱcolourȱandȱ digitalȱformats.ȱȱ Activity: Extension Age: High school Time: 3 Why? This activity measures the changes in coastal habitats that occur over a long period of time. Safety: This is a desk-based activity. Forȱthisȱactivityȱcurrentȱorȱhistoricȱorthophotoȱmapsȱorȱaerialȱphotographsȱmayȱbeȱused.ȱWhereȱ available,ȱtheseȱareȱtheȱtwoȱcheapestȱsourcesȱofȱspatialȱdataȱonȱyourȱestuary.ȱObtainedȱinȱanȱappropriateȱ scale,ȱusuallyȱbetweenȱ1:10,000ȱandȱ1:2500,ȱtheseȱmapsȱandȱphotographsȱcanȱbeȱusedȱtoȱidentifyȱlandȱ managementȱchanges,ȱnewȱareasȱofȱdevelopment,ȱchangesȱinȱvegetationȱorȱpollutionȱdischargeȱareas.ȱȱ Currentȱaerialȱphotos,ȱsatelliteȱimagery,ȱtopographicȱmapsȱandȱorthophotoȱmapsȱareȱproducedȱbyȱandȱ canȱbeȱpurchasedȱfromȱtheȱStateȱGovernment,ȱthroughȱMaplandȱSAȱatȱ(08)ȱ8463ȱ3999ȱorȱ <www.mapland.sa.gov.au>.ȱThisȱgroupȱalsoȱproducesȱCDȱROMsȱofȱaerialȱphotographsȱforȱdifferentȱ partsȱofȱtheȱstate,ȱandȱtheseȱareȱaȱveryȱeconomicalȱwayȱtoȱpurchaseȱlargeȱareasȱofȱcoverage.ȱ Thisȱactivityȱisȱsimplestȱtoȱcompleteȱifȱallȱtheȱdataȱsourcesȱareȱobtainedȱinȱsimilarȱscales,ȱasȱitȱmakesȱ comparisonȱeasier;ȱscalingȱcanȱbeȱdoneȱlaterȱifȱrequired.ȱTheȱpersonnelȱatȱMaplandȱorȱanyȱotherȱretailȱ outletȱshouldȱbeȱableȱtoȱassistȱyouȱinȱselectingȱtheȱrightȱscaleȱforȱthisȱactivity.ȱIfȱinȱdoubt,ȱaskȱtoȱlookȱatȱ anȱexampleȱofȱyourȱareaȱinȱeachȱofȱtheȱdifferentȱscalesȱandȱpickȱtheȱoneȱyouȱfeelȱbestȱfitsȱyourȱ requirements.ȱȱ What you may need: – aerialȱphotosȱofȱyourȱestuaryȱarea,ȱbothȱhistoricȱandȱrecentȱȱ – latitudeȱandȱlongitude,ȱAustralianȱMapȱGridȱorȱUniversalȱMapȱGridȱcoordinatesȱforȱaȱrangeȱofȱ landmarksȱsurroundingȱorȱinȱyourȱestuaryȱ(theseȱmayȱbeȱobtainedȱfromȱaȱgoodȱqualityȱ topographicȱmap)ȱ – clearȱtransparencyȱfilmȱ – blackȬlinedȱgraphȱpaper,ȱphotocopiedȱontoȱtransparencyȱfilmȱ – fineȬtippedȱpermanentȱmarkersȱȱ – topographicȱmapȱwithȱaȱsimilarȱscaleȱtoȱtheȱphotographȱ – rulerȱȱ – paperȱclips,ȱpinsȱorȱblueȱtackȱ – calculatorȱȱ – goodȱlight.ȱ ȱ 100 ESTUARINE MONITORING GUIDANCE MANUAL To ensure good results: Useȱatȱleastȱthree,ȱandȱpreferablyȱfour,ȱreferenceȱpointsȱtoȱcheckȱtheȱscaleȱofȱyourȱaerialȱphotographȱandȱ rememberȱtoȱgroundtruth—checkȱyourȱinterpretationȱbyȱvisitingȱtheȱsites.ȱ What to do: I nte rpr et ingȱ map sȱ andȱa e r ia l s:ȱ o r ie n t at i on ȱ Layȱyourȱphotographsȱandȱmapsȱoutȱinȱfrontȱofȱyou.ȱLocateȱlandmarksȱthatȱyouȱhaveȱvisitedȱonȱtheȱ ground.ȱCanȱyouȱfindȱany?ȱDoȱtheyȱlookȱdifferentȱfromȱhowȱyouȱthoughtȱtheyȱwouldȱlook?ȱȱ Layȱaȱtransparentȱsheetȱoverȱtheȱphotographsȱandȱmarkȱtheȱlandmarksȱwithȱaȱpermanentȱmarker.ȱWriteȱ theȱcoordinatesȱnextȱtoȱtheȱlandmarksȱonȱtheȱacetateȱsheet,ȱthenȱlayȱitȱoverȱaȱmapȱofȱtheȱsameȱscale.ȱIfȱ youȱdoȱnotȱhaveȱaȱmapȱofȱtheȱsameȱscale,ȱyouȱmayȱreduceȱorȱincreaseȱtheȱsizeȱofȱyourȱtransparencyȱonȱaȱ photocopier.ȱȱ Doȱtheȱlandmarksȱonȱtheȱtransparencyȱlineȱupȱwithȱthoseȱmarkedȱonȱtheȱmap?ȱHowȱfarȱoutȱareȱthey?ȱ Aerialȱphotosȱareȱdistortedȱdueȱtoȱtheȱcurveȱofȱtheȱearthȱandȱtheȱangleȱtheȱplaneȱwasȱatȱwhenȱtheȱphotoȱ wasȱtaken.ȱHowȱdistortedȱareȱyourȱphotos?ȱ I nte rpr et ingȱ map sȱ andȱa e r ia l s:ȱ tracing ȱobjectsȱo fȱ int er e st ȱ Placeȱaȱtransparentȱsheetȱontoȱyourȱaerialȱphoto.ȱSelectȱtheȱobjectsȱthatȱyouȱwishȱtoȱmap.ȱExamplesȱcouldȱ beȱvegetationȱtypes,ȱhousingȱandȱindustrialȱareas,ȱareaȱofȱtheȱestuaryȱorȱtheȱlocationȱofȱtheȱshoreline.ȱUseȱ yourȱfineȱtippedȱpermanentȱmarkersȱtoȱtraceȱaroundȱeachȱofȱtheȱobjectsȱofȱinterest,ȱusingȱaȱdifferentȱ colourȱifȱyouȱwantȱtoȱshowȱseveralȱthingsȱonȱtheȱsameȱsheet.ȱForȱinstance,ȱmangrovesȱcouldȱbeȱoutlinedȱ inȱoneȱcolourȱandȱsamphiresȱinȱanother.ȱ Beȱcareful,ȱwhenȱtracing,ȱtoȱbeȱasȱaccurateȱasȱpossible,ȱotherwiseȱyourȱdataȱwillȱnotȱbeȱofȱveryȱgoodȱ quality.ȱItȱisȱgenerallyȱrecommendedȱthatȱtheȱoutsideȱofȱyourȱmarkerȱlineȱmatchesȱtheȱoutsideȱofȱtheȱ objectȱorȱareaȱyouȱareȱmapping,ȱandȱthatȱtheȱlineȱisȱasȱfineȱasȱpossible.ȱȱ Ifȱmappingȱtheȱchangesȱthatȱoccurȱoverȱtimeȱinȱhabitats,ȱtraceȱeachȱofȱtheȱdifferentȱdatedȱaerialȱ photographsȱontoȱdifferentȱtransparentȱsheets.ȱȱ I nte rpr et ingȱ map sȱ andȱa e r ia l s:ȱworking ȱ out ȱa rea ȱ Onceȱyouȱhaveȱfinishedȱtracingȱallȱtheȱobjects,ȱreduceȱorȱenlargeȱthemȱonȱyourȱphotocopierȱuntilȱtheyȱareȱ allȱtoȱtheȱsameȱscale.ȱOnceȱyouȱareȱhappyȱwithȱthem,ȱphotocopyȱthemȱontoȱfilmȱthatȱhasȱgraphȱpaperȱ markingsȱonȱit,ȱorȱoverlayȱtwoȱbitsȱofȱfilmȱandȱstickȱthemȱtogether.ȱȱ Determineȱtheȱareaȱofȱeachȱofȱtheȱsquaresȱonȱtheȱfilm.ȱCountȱtheȱnumberȱofȱsquaresȱthatȱfallȱcompletelyȱ withinȱeachȱofȱtheȱtracedȱobjectsȱandȱestimateȱtheȱnumberȱofȱpartialȱsquares.ȱCalculateȱtheȱtotalȱareaȱ(onȱ theȱfilm)ȱofȱeachȱofȱtheȱtracedȱobjects.ȱMultiplyȱthisȱbyȱtheȱappropriateȱscaleȱtoȱgetȱtheȱareaȱofȱeachȱofȱtheȱ objectsȱonȱtheȱland.ȱȱ I nte rpr et ingȱ map sȱ andȱa e r ia l s:ȱ c omp ar ing ȱch ang e ȱ Changeȱcanȱbeȱmeasuredȱinȱaȱnumberȱofȱways.ȱItȱmayȱbeȱmeasuredȱasȱchangesȱinȱarea,ȱorȱitȱmayȱbeȱ measuredȱasȱchangesȱinȱlocation.ȱCompareȱyourȱtracingsȱandȱyourȱareaȱcalculations.ȱHasȱanyȱobjectȱorȱ landȱcoverȱtypeȱchangedȱsignificantlyȱinȱitsȱtotalȱarea?ȱAreȱtheȱindividualȱareasȱofȱvegetationȱsmallerȱorȱ larger?ȱȱ Layȱtheȱtracingsȱonȱtopȱofȱeachȱother.ȱHasȱanyȱobjectȱorȱlandȱcoverȱtypeȱchangedȱsignificantlyȱinȱ location?ȱIfȱsoȱhasȱitȱmovedȱinlandȱorȱseaward?ȱandȱbyȱhowȱmuch?ȱȱ Theseȱfiguresȱprovideȱveryȱusefulȱinformationȱforȱenvironmentalȱmanagers.ȱForȱexample,ȱinȱtheȱ Adelaideȱarea,ȱmuchȱofȱtheȱcoastalȱareaȱaroundȱPortȱAdelaideȱandȱGillmanȱhasȱpotentialȱacidȱsulphateȱ soils.ȱInȱtheȱ1890sȱseawallsȱwereȱputȱin,ȱtoȱkeepȱtheȱtideȱoutȱofȱlargeȱareasȱsouthȱofȱStȱKilda,ȱsoȱtheȱlandȱ couldȱbeȱusedȱforȱgrazing.ȱThisȱplanȱwasȱnotȱveryȱsuccessful,ȱandȱbyȱtheȱlateȱ1930sȱtheȱseawallsȱwereȱ breachedȱtoȱallowȱtheȱseaȱbackȱin.ȱHowever,ȱtheȱseaȱdidȱnotȱjustȱcomeȱbackȱinȱtoȱwhereȱitȱhadȱoriginally:ȱ inȱtheȱtimeȱitȱhadȱbeenȱkeptȱdry,ȱtheȱsoilȱhadȱoxidised,ȱgeneratingȱacid.ȱThisȱhadȱdissolvedȱmuchȱofȱtheȱ 101 ESTUARINE MONITORING GUIDANCE MANUAL carbonateȱ(shelly)ȱdepositsȱinȱtheȱsoil,ȱandȱtheȱlandȱhadȱshrunk.ȱItȱwasȱnowȱmuchȱlowerȱthanȱitȱhadȱbeenȱ whenȱtheȱseawallsȱwereȱfirstȱinstalled.ȱAsȱaȱresultȱtheȱmangroveȱzoneȱhasȱmovedȱinlandȱbyȱseveralȱ hundredȱmetresȱinȱsomeȱareas.ȱAcidȱsulphateȱsoilsȱareȱnotȱrestrictedȱtoȱtheȱAdelaideȱarea,ȱtheyȱoccurȱinȱ depositionalȱcoastalȱenvironmentsȱaroundȱtheȱstate.ȱActivitiesȱsuchȱasȱMeasuringȱsoilȱsalinityȱandȱsoilȱpHȱ willȱhelpȱyouȱdetermineȱifȱyourȱestuaryȱhasȱareasȱofȱacidȱsulphateȱsoils.ȱ I nte rpr et ingȱ map sȱ andȱa e r ia l s:ȱ facing ȱthe ȱground ȱtruthȱ ȱ Afterȱcompletingȱyourȱanalysisȱitȱisȱrecommendedȱthatȱyourȱdataȱbeȱgroundtruthed.ȱThisȱsimplyȱmeansȱ goingȱintoȱtheȱfieldȱwithȱaȱGPSȱandȱcheckingȱthatȱwhatȱyouȱthoughtȱyouȱsawȱonȱtheȱphotosȱorȱmapsȱisȱ actuallyȱthere.ȱȱ Markȱanyȱareasȱthatȱareȱdifferentȱfromȱyourȱoriginalȱassessmentȱandȱredoȱanyȱcalculationsȱbasedȱonȱthisȱ newȱdata.ȱȱ FurtherȱinformationȱonȱmappingȱmangrovesȱandȱsaltmarshȱcanȱbeȱfoundȱinȱWiltonȱ&ȱSaintilanȱ(2000).ȱȱ M or eȱd eta i le dȱa n a ly s is ȱ Ifȱyourȱgroupȱbecomesȱveryȱinterestedȱinȱanalysingȱaerialȱphotography,ȱyouȱmayȱbeȱpreparedȱtoȱspendȱ theȱtimeȱtoȱlearnȱtheȱuseȱofȱaȱcomputerisedȱgeographicȱinformationȱsystemȱ(GIS).ȱTheseȱpackagesȱ simplifyȱtheȱgeorectificationȱandȱscalingȱofȱyourȱphotographs,ȱandȱmayȱprovideȱveryȱsophisticatedȱ analyses.ȱFreeȱpublicȱdomainȱGISȱpackagesȱareȱavailableȱforȱthoseȱinterestedȱinȱexploringȱthisȱ technology.ȱMoreȱinformationȱ(andȱtheȱsoftware)ȱcanȱbeȱfoundȱatȱtheȱfollowingȱwebȱaddresses:ȱ — <www.microimages.com>ȱisȱtheȱsiteȱforȱfreeȱTNTliteȱsoftwareȱ(sizeȱlimitedȱversionȱofȱTNTmips)ȱ — <grass.baylor.edu>isȱtheȱsiteȱforȱfree,ȱopenȱsourceȱGRASSȱsoftwareȱdevelopedȱbyȱtheȱUSȱmilitaryȱ — <www.esri.com/software/arcexplorer>isȱaȱveryȱbasicȱfreeȱGISȱfromȱtheȱmakersȱofȱArcInfoȱ — <www.tiem.utk.edu/~sada>ȱisȱtheȱfreeȱSpatialȱAnalysisȱandȱDecisionȱAssistanceȱsoftwareȱpageȱ — <www.mapmaker.com>ȱisȱtheȱsiteȱtoȱdownloadȱMapmakerȱGratis.ȱ P r es ent ing ȱy ou rȱdata ȱ Dataȱfromȱthisȱactivityȱisȱpresentedȱasȱaȱlossȱorȱgainȱinȱhabitatȱareaȱorȱdevelopmentȱchangesȱoverȱaȱ period.ȱWhenȱdiscussingȱthisȱdataȱitȱisȱimportantȱtoȱmentionȱtheȱyearsȱbeingȱcomparedȱandȱtheȱscaleȱofȱ theȱoriginalȱphotographsȱorȱmaps.ȱȱ Aerialȱphotographȱinterpretationȱisȱsomewhatȱsubjective,ȱwhichȱisȱwhyȱgroundtruthingȱisȱveryȱ important.ȱ Theȱsignificanceȱofȱchangesȱmustȱbeȱassessedȱwhenȱinterpretingȱdataȱfromȱthisȱactivity.ȱChangesȱinȱlandȱ coverȱofȱlessȱthanȱ10%ȱoverȱ10ȱorȱ20ȱyearsȱmayȱnotȱbeȱsignificant.ȱTheȱerrorȱinȱtheȱprocessesȱusedȱmayȱ accountȱforȱaȱconsiderableȱamountȱofȱtheȱerror.ȱIfȱthereȱwasȱaȱconsistentȱ10%ȱchangeȱinȱlandȱcoverȱeveryȱ year,ȱforȱaȱperiodȱofȱ3–4ȱyears,ȱthisȱmayȱbeȱsignificant.ȱȱ Availabilityȱofȱphotographs,ȱorȱpoorȱqualityȱhistoricalȱphotos,ȱmayȱbeȱaȱdifficultyȱforȱsomeȱgroupsȱorȱ areas.ȱTheȱCoastȱandȱMarineȱBranchȱhasȱplacedȱaȱconsiderableȱquantityȱofȱcoastalȱhabitatȱmappingȱ (includingȱsomeȱhistoricalȱdataȱforȱpartsȱofȱtheȱSAȱcoast)ȱonlineȱinȱtheȱSouthȱAustralianȱnodeȱofȱtheȱ CoastalȱAtlas.ȱTheȱAtlasȱmayȱbeȱaccessedȱbyȱvisitingȱtheȱwebsiteȱatȱ<www.atlas.sa.gov.au/coastal/>.ȱȱ 102 ESTUARINE MONITORING GUIDANCE MANUAL Measuring beach litter LitterȱonȱourȱbeachesȱandȱfloatingȱinȱAustralianȱmarineȱandȱestuarineȱ watersȱisȱaȱbigȱproblem,ȱparticularlyȱnonȬbiodegradableȱlitter.ȱItȱ harmsȱourȱmarineȱlife,ȱdiversȱandȱbeachȬusers.ȱSoftȱdrinkȱringsȱmayȱ trapȱseabirds,ȱplasticȱbagsȱmayȱbeȱeatenȱbyȱseaȱmammalsȱorȱentangleȱ fish,ȱsharpȱneedlesȱandȱbitsȱofȱglassȱgiveȱnastyȱcutsȱorȱdiseasesȱtoȱ beachȬusers,ȱandȱbitsȱofȱnetȱentrapȱanythingȱfromȱhumansȱtoȱmarineȱ mammalsȱorȱfish.ȱCansȱfullȱofȱpropellantȱandȱheavyȱoilȱcauseȱminiȱ oilȬspillsȱthatȱmayȱkillȱsignificantȱcoloniesȱofȱbarnaclesȱorȱotherȱ marineȱlife.ȱAustralianȱbeachesȱareȱsomeȱofȱtheȱbestȱinȱtheȱworld,ȱandȱ litterȱmakesȱthemȱtoȱlookȱunsightly.ȱȱ Thisȱactivityȱencouragesȱvolunteersȱtoȱcollectȱallȱtheȱlitterȱtheyȱfindȱ alongȱaȱbeltȱofȱbeach,ȱrockȱembankmentȱorȱmudflat.ȱTheȱcollectedȱ litterȱisȱthenȱsortedȱintoȱdifferentȱcategoriesȱandȱweighed.ȱȱ TheȱinformationȱgainedȱfromȱthisȱactivityȱprovidesȱyourȱWaterwatchȱ coordinatorȱandȱKESABȱ(KeepȱSouthȱAustraliaȱBeautiful)ȱwithȱusefulȱ dataȱonȱtheȱamountȱofȱlitterȱbuildingȱupȱandȱalsoȱgivesȱyouȱaȱgoodȱ opportunityȱtoȱcleanȱupȱyourȱshoreline.ȱThisȱactivityȱcouldȱbeȱ undertakenȱasȱpartȱofȱCleanȬupȱAustraliaȱDayȱactivitiesȱeachȱyear.ȱIfȱ youȱwishȱtoȱregisterȱforȱCleanȬupȱAustraliaȱDay,ȱcontactȱKESABȱviaȱ theirȱwebsiteȱatȱ<www.kesab.asn.au>.ȱȱ AnotherȱusefulȱsourceȱofȱinformationȱisȱtheȱbrochureȱKeepingȱTabsȱonȱ MarineȱDebris.ȱTheȱbrochureȱandȱrecordȱformsȱareȱonȱtheȱinternetȱatȱ <www.environment.sa.gov.au/coasts/pdfs/marine_debris_info.pdf>.ȱȱ Toȱobtainȱtheȱmostȱvaluableȱdata,ȱyourȱstretchȱofȱbeachȱshouldȱbeȱ cleanedȱeveryȱthreeȱtoȱsixȱmonths,ȱalthoughȱonceȱaȱyearȱwillȱsuffice.ȱ Dataȱfromȱthisȱactivityȱcanȱhelpȱdetermineȱifȱeducationȱprogramsȱorȱ legislationȱtoȱreduceȱlitteringȱareȱworking,ȱidentifiesȱtheȱmajorȱ sourcesȱofȱlitterȱandȱhelpsȱraiseȱpublicȱawarenessȱofȱtheȱproblemȱofȱ litterȱinȱaquaticȱenvironments.ȱȱ What you may need: – beachȱlitterȱrecordȱformȱ – heavyȬdutyȱplastic,ȱhessianȱorȱCleanȬupȱAustraliaȱDayȱbagsȱȱ – bathroomȱscalesȱaccurateȱtoȱ0.2ȱkgȱ – 50Ȭmȱmeasuringȱtapeȱȱ – clipboardȱandȱdatasheetȱȱ – topographicȱmapȱorȱGPSȱ – specialȱcontainerȱforȱsharpsȱȱ – glovesȱandȱprotectiveȱshoesȱforȱeveryone.ȱȱ Activity: 3 Core Age: All Time: 2 or 3 Why? Many nonbiodegradable articles cause harm to marine life (entanglements and ingestion), people (diver entanglements, cuts to beach-users) and to marine vessels (propeller snags, etc). The estuarine benthic habitat may also become smothered with nonbiodegradable wastes. Safety: Ensure everyone is wearing gloves and protective shoes. As with all field work, ensure that a first aid kit is available. A yellow sharps container should be on hand to place all broken glass and needles into. If an object is large or heavy, ask for someone else to help you pick it up. Dead aquatic plants, animals and birds often wash up on the shore. Leave them there—they are part of the estuarine ecosystem and are usually meant to be there. Note large numbers of dead flora or fauna if the quantity seems unnatural. To ensure good results: Beachȱlitterȱcollectionȱisȱfairlyȱsubjective.ȱItȱdependsȱonȱwhatȱtheȱvolunteersȱconsiderȱtoȱbeȱlitter,ȱhowȱ thoroughȱtheȱsearchȱisȱandȱtheȱtimeȱofȱyearȱthatȱtheȱsurveyȱisȱdone.ȱAnotherȱlimitationȱisȱthatȱonlyȱ visibleȱlitterȱcanȱbeȱcollected.ȱSomeȱlitterȱmayȱbeȱburiedȱbyȱsand.ȱ 103 ESTUARINE MONITORING GUIDANCE MANUAL What to do: Selectȱaȱsuitableȱbeach,ȱmuddyȱshoreȱorȱrockyȱembankmentȱinȱyourȱestuary.ȱItȱshouldȱbeȱeasilyȱ accessible,ȱbeȱknownȱtoȱhaveȱaȱbuildȬupȱofȱlitterȱandȱhaveȱnoȱotherȱroutineȱcleaning.ȱEstuarineȱsitesȱ shouldȱbeȱfreeȱofȱmangrovesȱorȱotherȱstructures,ȱandȱbeȱclearȱofȱdirectlyȱdumpedȱrubbish.ȱDoȱnotȱ monitorȱbeachȱdunesȱforȱrubbish,ȱasȱtheyȱareȱfragileȱandȱmonitoringȱmayȱsometimesȱbeȱmoreȱharmfulȱ thanȱtheȱrubbish.ȱȱ Selectȱaȱstartingȱpointȱandȱlayȱoutȱaȱ500Ȭmȱtransectȱ(orȱasȱcloseȱasȱyouȱcanȱget)ȱusingȱaȱmeasuringȱtape.ȱAȱ 50Ȭmȱtapeȱmayȱbeȱused,ȱbyȱgettingȱseveralȱpeopleȱtoȱmeasureȱoutȱ10ȱlengthsȱofȱtheȱtape.ȱRecordȱtheȱ width,ȱstartingȱpoint,ȱlengthȱandȱdirectionȱofȱtheȱtransectȱonȱaȱmapȱorȱusingȱaȱGPS.ȱȱ Startȱwalkingȱalongȱtheȱbeachȱatȱtheȱbeginningȱofȱtheȱtransectȱandȱwalkȱinȱaȱcloseȱzigzagȱpattern,ȱ coveringȱtheȱentireȱareaȱofȱtheȱtransect.ȱCollectȱallȱrubbishȱandȱplaceȱintoȱaȱsuitableȱstrengthȱrubbishȱbag.ȱ Onceȱfinishedȱtakeȱallȱbagsȱfromȱtheȱbeachȱtoȱaȱsortingȱarea.ȱȱ Atȱtheȱsortingȱareaȱemptyȱallȱbagsȱandȱsortȱtheȱlitterȱintoȱtheȱrubbishȱtypesȱlistedȱonȱtheȱdatasheet.ȱCountȱ theȱnumberȱofȱlitterȱitemsȱinȱeachȱcategoryȱandȱrecordȱthem.ȱȱ Brushȱsandȱorȱmudȱfromȱeachȱitemȱofȱlitterȱusingȱaȱbrushȱ(orȱhoseȱoff)ȱandȱplaceȱintoȱplasticȱbagsȱ labelledȱwithȱtheȱtypeȱofȱrubbishȱtheȱbagȱcontains.ȱWeighȱallȱtheȱbagsȱforȱeachȱcategoryȱusingȱyourȱ scales.ȱRecordȱtheȱweightsȱforȱeachȱcategoryȱonȱyourȱsheet.ȱȱ Foamȱorȱotherȱlightȱitemsȱcanȱbeȱputȱintoȱbagsȱofȱaȱknownȱsizeȱratherȱthanȱcleaningȱandȱweighingȱthem.ȱ Recordȱtheȱnumberȱandȱsizeȱofȱtheseȱbagsȱonȱyourȱdatasheet.ȱFishingȱlineȱcanȱbeȱrecordedȱinȱitsȱ approximateȱlengthȱratherȱthanȱbeingȱweighed.ȱȱ Disposeȱofȱtheȱdebrisȱitemsȱintoȱaȱrubbishȱskipȱorȱatȱaȱrubbishȱtip.ȱWhereȱpossible,ȱrecycleȱrubbish.ȱȱ Youȱmayȱalsoȱrecordȱtheȱpresenceȱofȱwashedȱupȱmarineȱorganicȱmatterȱ(egȱseagrassesȱorȱalgae)ȱonȱyourȱ recordȱsheet.ȱȱ Separatingȱlitterȱintoȱcategoriesȱmakesȱanalysingȱtheȱdataȱeasier.ȱItȱensuresȱthatȱtheȱsourceȱandȱ transportationȱmethodsȱofȱeachȱofȱtheȱrubbishȱcategoriesȱcanȱbeȱassessed.ȱHaveȱaȱtryȱatȱguessingȱtheȱ sourcesȱofȱyourȱrubbish.ȱWhereȱdoȱyouȱthinkȱitȱcameȱfrom?ȱWhatȱcluesȱareȱgivenȱaboutȱhowȱitȱgotȱtoȱ yourȱtransectȱarea?ȱDoȱyouȱthinkȱthatȱthereȱwasȱanȱacceptableȱamountȱofȱlitterȱonȱtheȱbeach?ȱ Dataȱisȱbetterȱinterpretedȱasȱweightȱofȱlitterȱperȱlengthȱofȱbeachȱ(ratherȱthanȱweightȱperȱunitȱarea)ȱdueȱtoȱ theȱtendencyȱforȱmuchȱbeachȱlitterȱtoȱbeȱclumpedȱalongȱaȱnarrowȱbandȱatȱtheȱhighȱtideȱmark.ȱItȱisȱbetterȱ toȱuseȱweightȱasȱanȱindicatorȱthanȱtotalȱnumberȱofȱitems,ȱasȱmanyȱitemsȱtendȱtoȱbreakȱupȱintoȱsmallerȱ fragments.ȱȱ Beachȱlitterȱmonitoringȱisȱbestȱconductedȱeveryȱoneȱtoȱthreeȱmonths.ȱPleaseȱnoteȱthatȱincreasedȱ quantitiesȱofȱlitterȱareȱlikelyȱtoȱbeȱfoundȱafterȱstrongȱwinds,ȱcurrentsȱorȱstorms.ȱAlwaysȱcollectȱlitterȱatȱ lowȱtideȱwhenȱtheȱbeachȱareaȱisȱexposed.ȱȱ ȱ ȱ 104
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