i STANDARDISATION OF FALOODA By Mr. MORE VIKRANT SAHADEO (Reg.No. R/013/081) A Thesis submitted to the MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722, DIST. AHMEDNAGAR, MAHARASHTRA, INDIA In partial fulfilment of the requirements for the degree Of MASTER OF SCIENCE (AGRICULTURE) In DAIRY SCIENCE DEPARTMENT OF ANIMAL HUSBANDRY AND DAIRY SCIENCE POST GRADUATE INSTITUTE, MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722, DIST. AHMEDNAGAR, MAHARASHTRA, INDIA 2015 ii STANDARDISATION OF FALOODA By Mr. MORE VIKRANT SAHADEO (Reg.No. R/013/081) A Thesis submitted to the MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722, DIST. AHMEDNAGAR, MAHARASHTRA, INDIA In partial fulfilment of the requirements for the degree Of MASTER OF SCIENCE (AGRICULTURE) In DAIRY SCIENCE Approved by Dr. D. H. Kankhare (Chairman and research guide) Dr. V. P. Kad Dr. R. J. Desale (Committee member) (Committee member) Dr. D. P. Kaledhonkar (Committee member) DEPARTMENT OF ANIMAL HUSBANDRY AND DAIRY SCIENCE POST GRADUATE INSTITUTE, MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722, DIST. AHMEDNAGAR, MAHARASHTRA, INDIA 2015 iii CANDIDATE’S DECLARATION I hereby declare that this thesis or a part there of has not been submitted by me or any other person to any other University or Institute for a Degree or Diploma Place: M.P.K.V., Rahuri Date : / / 2015 (V. S. More) iv Dr. D. H. Kankhare Assistant Professor, Division of Animal Husbandry And Dairy Science, College of agriculture, Dhule, Mahatma Phule Krishi Vidyapeeth, Rahuri - 413 722, Dist. Ahmednagar, Maharashtra, India. CERTIFICATE This is to certify that the thesis entitled, “STANDRDISATION OF FALOODA”, submitted to the Faculty of Agriculture, Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar, M.S. for the award of the degree of MASTER OF SCIENCE (AGRICULTURE) in DAIRY SCIENCE, embodies the results of a bona fide research carried out by Mr. MORE VIKRANT SAHADEO, under my guidance and supervision and that no part of the thesis has been submitted for any other Degree or Diploma. Place: M.P.K.V., Rahuri Dated: / /2015 (D. H. Kankhare) Research Guide v Dr. B.R. Ulmek Associate Dean, Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri - 413 722, Dist. Ahmednagar, Maharashtra, India. CERTIFICATE This is to certify that the thesis entitled, “STANDARDISATION OF FALOODA ”, submitted to the Faculty of Agriculture, Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar, M.S. for the award of the degree of MASTER OF SCIENCE (AGRICULTURE) in DAIRY SCIENCE, embodies the results of a bona fide research carried out by Mr. MORE VIKRANT SAHADEO under the guidance and supervision of Dr. D. H. KANKHARE, Professor, Department of Animal Science and Dairy Science, M.P.K.V., Rahuri and that no part of the thesis has been submitted for any other Degree or Diploma. Place: M.P.K.V., Rahuri Dated: / /2015 (B. R. Ulmek) vi ACKNOWLEDGEMENT I avail this opportunity to acknowledge all those who helped and guided me during the course of my research work. Before giving way to my feelings, I cordially want to salute the Supreme cosmic consciousness from which everything originates in the beginning and to which everything goes to end. Though formal words cannot carry the fragrance of emotions with them, still they are the only available way of expressing the emotions. Words are too meagre to express my esteem indebtedness and whole hearted sense of gratitude towards my honorable Research guide and chairman of my Advisory Committee, Dr. D .H. Kankhare, Assistant Professor of Animal Husbandry and Dairy Science, college of agriculture, Dhule. It is my pleasure beyond words to express my deep sense of feelings for his benevolent guidance, meticulous supervision, whole hearted encouragement, critical appreciation in execution of my work and for all the trust he had in my ability primarily responsible for the present accomplishment. Really, fortunate I am and I feel extremely honored for the opportunity conferred upon me to work under his perpetual motivation. Grateful thanks to Dr. T. A. More, Hon. Vice-Chancellor, M.P.K.V, Rahuri and Dr. B. R. Ulmek, Associate Dean, Post Graduate Institute, MPKV, Rahuri. The words at my command are in adequate in forms as vii well as spirit to convey the depth of my feelings of gratitude to Dr. Y. G. Fulpagare, Head, Department of Animal Husbandry and Dairy Science, M.P.K.V., Rahuri for his keen interest and encouragement during this research work. With a profound and unfading sense of obligation I express my heartful thanks to the member of my Advisory Committee Dr. R. J. Desale, Assistant Professor, Department of Animal Husbandry and Dairy Science, Dr. V. P .Kad, Assistant Professor, Post Harvest Technology Unit, MPKV Rahuri and Dr. D.P. Kaledhonkar , Assistant professor , Division of Statistics, College of Agriculture, Pune, MPKV Rahuri for their valuable suggestions and encouragement during the research work and critical reviewing of manuscript. I again earnestly extend my thanks to Prof. B. B. Khutal, Dr. U. Y. Bhoite, Dr V. S. Lawar and Dr. D. D. Patange for their constructive criticism and suggestions especially during organoleptic evaluation of falooda samples. I would like to take this opportunity to convey my sincere thanks to all other respected teachers and non- teaching staff of the Animal Husbandry and Dairy Science for their cooperation. I express my regards to Prof. P. A. Shinde, University Librarian, M.P.K.V., Rahuri and his office staff for their kind co-operation during the course of my post graduate study. It is my ethereal pleasure to convey my heartfelt reverence to my beloved parents, who have been an inexhaustible viii source of inspiration throughout my life. Mere acknowledgement will never be enough for their unfading sacrifice, love and good wishes which nourishes my hopes and ambitions. I express my deep sense of appreciation towards my sisters Manisha (Tai), Rani (Aabu) and my brother Rajanikant, for their never ending love, constant encouragement and eternal moral support which have brought this cherished dream to reality. Research work in laboratory doesn't goes on right path without valuable tips and suggestions of senior associate I will feel guilty if I don’t thank my beloved seniors Patil sir, Ranjit sir, Balaji sir, Pankaj sir, Kalpesh sir, Sayali mam, Manoj sir, Amol sir, Chandu sir, Dnyansagar sir and Prasad sir who rendered their sumptuous help during the course of this investigation. The words are small trophies to express my feelings of affection and indebtedness to my seniors Mangesh sir, Ajay sir , my colleagues Bhaskar, Abhijeet, Tushar, Amit and my juniors swapnil, sachin, Akshay, Umesh And Mahesh. I express my heartful thanks to my classmates Yuvraj, Bhagyashree, Mohini, Balasaheb, Nitesh, Kalyani and to my juniors Prasad, Yatish, Rohan and Jaideep. My heart is filled with the sweet memories of my friends Raj, Ulhas, Madan, Shankar, Sandip, Deepak, V ishal Amol and for their constant encouragement and moral support during the completion of my research work, comforting me at the times of despair and constant inspiration never gave me a chance ix to fallen in this Endeavour. I would be failing to in my duties if I don’t record deep sense of appreciation for the wholehearted co-operation and assistance provided for completing this work by Mrs. Landage Madam, Laboratory Assiastant, Department of Animal Husbandry and Dairy Science MPKV, Rahuri and shri. Mhase and Santosh mama. I am also obliged to all authors past and present whose literatures have been cited in this manuscript. Finally, I am thankful to Mahatma Phule Krishi Vidyapeeth, Rahuri for provided me this opportunity to undertake the post graduate studies in this institute of national repute. Many have not been mentioned but none is forgotten. Place: MPKV, Rahuri. Date: / / 2015 (V. S. More) x CONTENTS CANDIDATE’S DECLARATION iii CERTIFICATE a. Research Guide iv b. Associate Dean (PGI) v ACKNOWLEDGEMENT vi LIST OF TABLES xiv LIST OF FIGURES xv LIST OF PLATES xv LIST OF ABBREVIATIONS xvi ABSTRACT xvii 1. INTRODUCTION 1 2. REVIEW OF LITERATURE 7 2.1 Vermicelli 7 2.1.1 Use of vermicelli in milk products 9 2.1.2 Chemical composition of vermicelli 9 2.1.3 Vermicelli made from different flours 11 2.1.4 Sensory evaluation of vermicelli 12 2.2 Basil seeds 14 2.2.1 Nutritional and therapeutic importance of basil seeds 2.2.2 Use of basil seeds 15 2.2.3 Chemical composition of basil seeds 18 2.3 Falooda 2.3.1 Chemical composition of falooda 17 19 20 xi 2.4 Similar Products 20 3.1 Materials 23 3.1.1 Glass wares 23 3.1.2 Electric Balance 23 3.1.3 Muffle furnace 23 3.1.4 Mixer / grinder 23 3.1.5 Vessel 23 3.1.6 Chemicals 24 3.2 Ingredients 24 3.2.1 Milk 24 3.2.2 Sugar 24 3.2.3 Vermicelli 24 3.2.4 Basil seeds 24 3.3.5 Dry fruits 24 3.3.6 Cheery 24 3.3 Methods 3.3.1 Chemical analysis of milk 25 25 3.3.1.1 Fat 25 3.3.1.2 Total solids 25 3.3.1.3 Acidity 25 3.3.1.4 Protein 25 3.3.1.5 Lactose 25 3.4 Pre- Experimental details 26 3.4.1 Selection of levels of different ingredients 26 3.4.2 Selection of levels of vermicelli and basil seeds. 26 3.4.3 Selection of levels of sugar and level of reduced 28 milk 3.4.4 Selection of levels of dry fruits 28 3.4.5 Selection of levels of rose syrup and cherries 28 xii 3.5 Experimental trials 29 3.5.1 Treatment Details 29 3.5.2 Technique for preparation of falooda 30 3.5.3 Flow chart of falooda preparation 31 3.5.4 Addition of ingredients 32 3.5.4.1 Addition of vermicelli 32 3.5.4.2 Addition of basil seeds 32 3.5.4.3 Addition of dry fruits 32 3.5.4.4 Addition of rose syrup 33 3.3.4.5 Garnishing of the product 33 3.6 Sensory of evaluation of falooda 3.7 Chemical analysis of falooda 33 33 3.7.1 Fat 33 3.7.2 Protein 34 3.7.3 Lactose 35 3.7.4 Ash 36 3.8 Statistical analysis 36 3.9 Cost of preparation of the falooda 36 4. RESULT AND DISCUSSION 37 4.1 Chemical composition of milk 37 4.2 Chemical composition of falooda samples 38 4.2.1 Fat 38 4.2.2 Protein 40 4.2.3 Lactose 41 4.2.4 Ash 43 xiii 4.3 Chemical composition kheer as per De et al. (1980) 44 4.4 Sensory evaluation of falooda samples 45 4.4.1 Colour and appearance 45 4.4.2 Flavour 48 4.4.3 Consistency 50 4.4.4 Overall acceptability 51 4.5 Cost of preparation of falooda 52 5. SUMMARY AND CONCLUSION 54 5.1 Pre – experimental trials 54 5.2 Chemical quality of falooda 55 5.3 Sensory evaluation of falooda 56 5.4 Cost of preparation of falooda. 57 5.2 Conclusion 57 6. LITERATURE CITED 59 7. APPENDIX 69 8. VITA 71 xiv LIST OF TABLES No. Title Page No. 1 Chemical composition of vermicelli 10 2 Chemical composition of basil seeds 19 3 Chemical composition of falooda 20 4 Treatment details 29 5 Chemical analysis of milk 37 6 7 8 9 10 11 12 13 14 15 Effect of treatment combinations on Fat content 38 of falooda samples Effect of treatment combinations on protein 40 content of falooda samples Effect of treatment combinations on lactose 41 content of falooda samples Effect of treatment combinations on ash content 43 of falooda samples Composition of kheer as per De et al (1980) 44 Effect of treatment combinations on Score for 45 colour and appearance of falooda samples Effect of treatment combinations on Score for 48 flavour of falooda samples Effect of treatment combinations on Score for 50 consistency of falooda samples Effect of treatment combinations on Score for 51 overall acceptability of falooda samples The cost of preparation of falooda 53 xv LIST OF FIGURES Sr. no 1 2 3 4 5 6 7 8 Title Effect of treatment combinations on Fat content of falooda samples Effect of treatment combinations on Protein content of falooda samples Effect of treatment combinations on Lactose content of falooda samples Effect of treatment combinations on Ash content of falooda samples Effect of treatment combinations on Score for colour and appearance of falooda samples Effect of treatment combinations on Score for flavour of falooda samples Effect of treatment combinations on Score for consistency of falooda samples Effect of treatment combinations on Score for overall acceptability of falooda samples Between Pages 40-41 40-41 43-44 43-44 48-49 48-49 51-52 51-52 LIST OF PLATES No. Title Between pages 1 Ingredients used 32-33 2 Six different treatment combinations 45-46 xvi LIST OF ABBREVIATIONS CD : Critical Difference et al., : And other (et alli) Fig. : Figure g : Gram (s) h : Hour (s) i.e. : That is lit. : Liter mg : Milligram ml : Milliliter N : Normality No. : Number (s) S. E. : Standard Error SNF : Solid Not Fat TS : Total Solids Viz. : Namely Wt. : Weight µ : Micron 0C : Dégrée centigrade % : Percent WPC : Whole Plate Count CHO : Carbohydrates BSG : Basil Seed Gum IU : International Unit xvii ABSTRACT STANDARDISATION OF FALOODA By Mr. MORE VIKRANT SAHADEO A candidate for the degree of MASTER OF SCIENCE (AGRICULTURE) In DAIRY SCIENCE 2015 Research Guide Discipline Major Field : Dr. D. H. Kankhare : Animal Husbandry and Dairy Science : Dairy Science In the present study entitled “Standardization of falooda” was carried out in laboratory of Department of Animal Husbandry and Dairy Science, Post Graduate Institute, M.P.K.V., Rahuri. The main objective of this research work was to finalize the levels of vermicelli and basil seeds to be used in preparation of falooda. The levels of basil seeds and vermicelli in falooda decide the taste and acceptability of the said product. The chemical attributes determined were fat, protein, lactose and ash content of falooda. The sensory evaluation was carried out in respect of colour and appearance, flavour, consistency and overall acceptability. xviii Abstract contd… Mr. V. S. MORE Before selection of levels of vermicelli and basil seeds, their standardization was done in pre-experimental trials and best accepted levels of vermicelli (3%, 3.5%, 4%) and basil seeds (0.4% and 0.6%) were finalized. The falooda samples were prepared using cow milk from Research cum Development Project (RCDP) on cattle, MPKV, Rahuri, Dist. Ahmednagar. The sugar level was finalized in pre-experimental trials to 10 percent which was accepted by panel of judges. The data generated was laid out in two factorial completely randomized designs (FCRD). The combination V2B2 with 3.5% of vermicelli and 0.6% of basil seeds showed best overall acceptability than others. The combination V3B2 showed highest percentage of fat (7.32%), protein (10.05%), lactose (10.12%) and ash (1.20%). Thus addition of vermicelli (3.5%) and basil seeds (0.6%) gives good colour and appearance, good flavour, best consistency and overall acceptability over other combinations. The cost of production was estimated based on market prices of the ingredients used for falooda preparation. The Rs/kg cost for best liked combination of 1kg falooda was Rs 119. It may be concluded that falooda can be prepared with 3.5% of vermicelli and 0.6% of basil seeds to increase the acceptability of falooda by improving its sensory and chemical quality. Pages 1 to 71 1 1. INTRODUCTION The traditional dairy products reflect the intimate relationship that our ancestors established with the farm and animal wealth. The relationship continuing even today links us with our early ancestors. In ancient India, dairy products had an important bearing on the socio-cultural life of the people. They were indicators of the wealth and status of the people. Besides their use in daily religious rituals, these products were also used for medicinal purposes in ancient ayurveda as well as household remedies (Iya, 2002). From the time immemorial, India‟s traditional foods with their extra ordinary variety and richness have served people‟s need for nutrition and sound health. Within this wide range, dairy products from different regions of the country have provided a wide array of sweets and other specialties that are gourmet‟s delights, these also meet the need for proteins and calcium in people‟s daily diet. The story of Indian mithias is literally of epic proportions. Although the processes of making sweets have undergone continuous change, the time has now come to integrate traditional methods with culinary technology to meet consumer demands for better standardized quality, longer shelf life and greater convenience (Patel, 2002). Indian products have been source of joy for ages, much need to be done to give them the importance they deserve in the global market place. Growing income and consumerism have also stimulated not only a greater demand for these products but also provided a unique opportunity for their 2 systematic study, research and documentation relating to their origin, technology and standards. Today Indian milk products are the largest and fastest growing segment of the dairy industry. They offer opportunity for absorbing the growing milk surplus, generated by the operation flood. Over the millennia, traditional milk products of India have enriched the cuisine of this vast sub continent. These products constitute a large array of sweet, tempting, indulging confections. They have added colour, taste, and flavor to life and have become synonymous with feasts, festivals and celebrations. More and more of these milk delicacies have become commercially significant for the organized sector. A large number of them continue to be made on a cottage scale in traditional way. Many traditional foods have remained unchanged with regard to processing or packaging for centuries, due to the fact that they developed in a particular location and are deep rooted in the natural, cultural, religious and socio-economic environment. Some have disappeared without a trace as a result of modern influences, while some have expanded on global scale, becoming household products (Patel and Jha, 2002). In India, urbanization is taking place speedily. Traditional processes of preparing different dairy culinary arts and skills and are often inconvenient. Therefore there is a need to simplify and standardize the process of preparing traditional dairy products. (Kadam et al., 1998). 3 Falooda is popular beverage in Indian subcontinent, made primarily by mixing rose syrup with vermicelli and basil seeds along with either milk or water. Falooda is named for the strands of corn flour vermicelli that float in flavoured milk based drink. Falooda appears quite similar to sevian and Chinese noodles. It is soft gelatinous, thread like and translucent. It can be coloured with natural colouring materials like saffron, rose, etc. Typical falooda is a milk based drink, flavoured with rosesyrup and vermicelli strands floating in it. It is topped with ice cream or kulfi and decorated with dry cherries (Patel and Arora, 2005). Falooda is quite similar to famous Thai drink known a “NAM MANGLAK”, this drink is made of mixture of basil seeds, rose water, water and sugar. In Iraq, falooda is made with thicken kind of vermicelli. Bubble tea is a similar kind of falooda drink in East Asia. Falooda is milk based beverage and most beverages typically contain a lot of water and while not necessarily adding nutrients to the diet. They importantly help to prevent dehydration. Many fruit drinks contain significant sugar, adding energy to the diet and in some cases provide vitamins and minerals (Anonymous. 2012). Basil seed is one of the important ingredients in falooda. Basil (Ocimum basilicum L.) is an annual spicy herb, indigenous to India which has been cultivated for several millennia for its aromatic and medical uses (Pushpangadan, 2012). 4 Sweet basil (Ocimum basilicum L.) is well known and appreciated spice and medicinal plant. Apart from biologically active compounds, such as volatile oils, tannins, terpens, flavoinoids or glycosides, it is also a valuable source of macroand micro elements (Kohlmunzer, 2003). Rich chemical composition as well as unique taste and flavour have recently gained basil its considerable position and wide range use in polish cuisine. Basil‟s leaves are used as spiced both when fresh and after being frozen or dried. The sweet basil seeds do resemble the chia seeds and is known by the names Sabja seeds or falooda seeds or Tukmaria in India. They are basically the seeds of the sweet basil. Sabja seeds are most commonly used in making falooda and can be easily added to sherbets, milkshakes and other drinks. Although these sweet basil seeds do not have taste of their own but they give a good texture to the dish and make it healthy too. Vermicelli is another major ingredient used in falooda preparation. Vermicelli, a kind of pasta, is a traditional type of pasta round in section similar to spaghetti. The vermicelli noodles are used in number of dishes including a variation of kheer a sweet dessert similar to rice pudding. The falooda is a Persian frozen dessert made with thin vermicelli noodles, frozen cornstarch, rose water, lime juice and often ground pistachios (Anonymous, 2006). Vermicelli is a product prepared by using whole or refined wheat flour. Hard dough is prepared, extruded and dried in the sun. Since wheat flour is deficient in lysine, one of the 5 essential amino acids, the protein quality remains poor. Therefore, value addition of vermicelli is of prime importance to improve nutrient content and to save its delicacy. Secondly, use of value added processed foods can be a solution to the problem of supplementary feeding and under nutrition. (Mogra and Midha, 2013). At present many of the regional specific milk products have been overlooked and neglected, falooda is one of them. This product can enhance the nutritional importance of our diet at very cheap rate. As such products like falooda do not have any recommended standards in dairy industry. Looking towards the enormous acceptance of this product and wide market for it, there lies need to specify the standard for this product. Falooda at present is sold in variety of combinations like kulfi falooda, kesar falooda, and ice cream falooda. The number of ingredients (fruits, colours, flavours etc.) used in preparation varies according to place and consumer‟s demand. But the basic ingredients viz; vermicelli, basil seeds and sugar are found to be common. The product falooda has popular place in Indian beverage industry and holds a majestic place in the consumer‟s heart. The product is famous in all age groups, all religions and at all locations of the world by different names and recipe. By seeing the wider acceptability and scope of product, it seems, there is a great need to standardize the use of ingredients, in amount and variety, considering the nutritional importance, marketability and scope of product. 6 Thus, the present research work was laid down with following objectives: 1. To study and standardize the levels of basil seeds and vermicelli in falooda preparation. 2. To carry out sensory evaluation studies for different treatments combination. 3. To work out the physico - chemical properties and cost structure of the product. 7 2. REVIEW OF LITERATURE The manufacture of indigenous milk products is confined mainly to the non – industrial sector in India. A major portion of the milk produced in the country is converted into sweets and desserts, which are deeply rooted in ancient traditions and have a strong cultural heritage. Centuries of tradition and culture have evolved firmly established consumption habits amongst the population. (Jha, 2000). The literature reviewed under different heads intends to cover various aspects of falooda like technology, ingredients involved, their status, significance and therapeutic importance. 2.1 Vermicelli “Vermicelli is a product prepared by using whole or refined wheat flour comprises a good percentage of carbohydrate with sugar, fiber, minerals and vitamin”. Mogra and Midha (2013) stated that, vermicelli is a product prepared by using whole or refined wheat flour. Hard dough is prepared, extruded and dried in the sun. Since wheat flour is deficient in lysine, one of the essential amino acids, the protein quality remains poor. Therefore value addition of vermicelli is of prime importance to improve nutrient content and to save its delicacy. Secondly, use of value added processed foods can be a solution to the problem of supplementary feeding and under nutrition. 8 (Anonymous, 2012).Vermicelli has high nutrional value, are easy to digest and rich in taste. Nutrionally vermicelli noodles are calorie dense and high in carbohydrates but fat free and low in sodium. Niturkar et al. (1992) conducted studies on the formulations and quality attributes of milk protein based vermicelli (seviah) for kheer like product. Where in, they stated that, as the level of milk protein fortification was increased, the water required for dough formation got decreased and time for dough development and extrusion increased. Fortification of milk protein increased the vermicelli thread length with improvement in colour and texture. An improvement in sensory qualities of vermicelli was brought about with 4 % milk protein fortifications in either of the wheat flour fractions. Indian desserts including kheer are prepared by partial dehydration of whole milk together with sugar and cereals like rice or wheat or semolina or vermicelli (seviah). Joglekar et al. (1990) prepared vermicelli from wheat flour dough with fortification of milk protein and addition of salt @ 1 per cent by using hot water and steam conditioning process. The extruded length of vermicelli thread notably exhibited the lower range of 40-42 cm in case of semolina fractions incorporated with milk protein. However, the wheat flour incorporated with milk proteins @ 2 to 6 per cent, occurred in the higher range of average thread length from 50 to 47 cm respectively. In general a reduction in the thread length was noticed with increased milk protein fortification. 9 Altschul (1976), reported that protein based foods with dairy products processed along with oil seeds or cereal foods would provide excellent nutrition at reasonable price. 2.1.1 Use of vermicelli in milk products Joglekar et al. (1990) reported the proximate analysis of kheer made with the incorporation of 8 per cent (skim milk fortified) vermicelli (seviah) and 12 per cent sugar, as 69.64, 30.36, 10.39, 2.03, 0.190, 16.08, 1.67 per cent, respectively of moisture, dry matter, protein, fat, crude fiber, carbohydrate (including sugar) and ash. Niturkar (1989) reported that the kheer made from vermicelli (seviah) contain protein, carbohydrate and fat as 13.95 g, 69.63 g, and 2.035/100g respectively. Similarly, Kodgire (1990) also reported the composition of kheer made from vermicelli, which contains pH, acidity of skim milk, phosphorus, lysine, calcium, magnesium and iron as 6.43, 0.132 per cent, 293.30 mg/100 g, 470.60 mg/100 g, 2.370 g/100 g, 41.66 mg/100 g and 0.144 mg/100 mg, respectively. 2.1.2 Chemical composition of vermicelli Mogra and Midha (2013) prepared vermicelli using whole wheat flour, malted wheat flour and malted wheat flour + gram dhal + spinach + sago flour. They reported that moisture as 7.7, 6.9, 7.6, protein 9.3, 9.4, 13.5, ash 2.9, 4.8, 5.8, crude fiber 2.2, 2.3, 2.4, carbohydrate 75.2, 74.9, 69.8 and fat 2.7, 1.7, 1.2 per cent, respectively. Kulkarni (2012) developed nutrient rich noodle by supplementation with malted ragi flour and reported that 10 moisture 7.12, protein 9.93, fat 15.8 and crude fiber 0.3 per cent respectively. Baskaran et al. (2011) analyzed chemical attributes of noodles supplemented with 5, 7.5 and 10 per cent skim milk powder and reported that protein as 14.59, 15.79, 16.67,per cent carbohydrate 83.78, 82.59, 82.21,per cent fat 0.55, 0.56, 0.54 per cent, fiber 0.21, 0.20, 0.19 per cent and ash 0.87, 1.24 and 1.40 per cent, respectively. Similarly, pasta prepared using semolina, chick pea flour and defatted soy flour contains moisture 11.66, 10.99, 7.14percent, ash 0.80, 2.59, 5.93per cent, fat 1.02, 4.57, 0.77per cent, carbohydrate 73.92, 59.00, 35.84 per cent, respectively reported by Bashir et al. (2012). Sood et al. (2009) studied quality evaluation of wheat : fruited pasta products made from blendes of 20% jamun and papaya pulp and reported that moisture as 9.17, 9.94, 9.86 per cent, ash 1.87, 1.85, 1.81,per cent crude protein 12.58, 13.09, 13.00,per cent crude fat 2.29, 2.39, 2.36 and crude fiber as 2.23, 2.74 and 2.71 per cent respectively . Table1. Chemical composition of vermicelli Doke et al. (1990). Constituent Moisture Dry matter Protein Fat Crude fiber Carbohydrate Ash Per cent 11.42 88.58 13.76 0.19 0.72 71.97 1.94 11 Adsule (1985) carried out extensive review of the chemical composition of wheat. He mentioned that wheat contains about 1.8 per cent of the mineral matter or ash. 2.1. Vermicelli made from different flours Bashir et al. (2012) made noodles using durum semolina, sample two contained semolina (84%), chickpea flour (10%) and defatted soy flour (0%), sample there contained semolina (76%), chickpea flour (14%) and defatted soy flour (10%) and the fourth sample contained semolina (68%) gram flour (18%) and defatted soy flour (14%). All the samples were passed through sieve (10 meshes) thrice to improve mixing. Kulkarni (2012) prepared vermicelli from malted ragi flour and wheat flour to improve the nutritional quality of instant vermicelli without deteriorating their organoleptic properties. Similarly, Morga and Midha (2013) reported that vermicelli prepared from three variations whole wheat flour (100 per cent), malted wheat flour (100 per cent) and malted wheat flour + gram dhal + spinach + sago flour (50.9, 33.0, 2.1 and 14 per cent, respectively). Textural and rheological properties of whey protein concentrate fortified baked product from cassava based flours, was studied by Jisha et al. (2009). Similarly, Sood et al. (2009) also prepared fruit noodles by using wheat flour blended with 20% jamun and papaya fruit. Fu (2008) made ragi supplemented noodles using wheat flour and malted ragi flour with different combination. 12 Constandache et al. (2005) also prepared dough using wheat flour fortified with sodium caseinate at 2 per cent, 4 per cent and whey protein concentrate at 5 per cent and 10 per cent level for preparation of dough for bakery products. Tripathy et al. (2003) reported that ragi based products of local interest were formulated utilizing WPC to enhance their nutritional profile. Spray dried WPC (40 per cent protein) was used at 10 to 40 per cent to replace ragi flour and the products such as ragi malt and ragi dosa were prepared by using the regular recipe. Swaminathan (1987) developed vermicelli using wheat flour and reconstituted by boiling water with weighted amount of spice mix. Such as chilli turmeric, cumin, coriander, onion, garlic, garam masala and sugar. Also Kothule (1990) made vermicelli with wheat flour and fortified with 2%, 4% and 6% level of dried skim milk powder. Raghavendra (1979) reported that roti, dosa and vermicelli prepared from unconventional sources such as maize, sorghum and, bajra flour. Water needed for making dough, baking time, moisture in baked roti, chewing characteristics and storage (24 hours) quality were assessed for roti and vermicelli. 2.1.4 Sensory evaluation of vermicelli Mogra and Midha (2013) prepared vermicelli using whole wheat flour, malted wheat flour and malted wheat flour + green gram dhal + spinach + sago flour in different levels. They found that vermicelli prepared from malted wheat flour, green 13 gram dhal, spinach, sago flour vermicelli was highest in protein content (13.5%) while energy content was high in whole wheat flour. Malted wheat flour vermicelli had highest amount of total (8.91%), reducing (2.41%) and non- reducing sugars (6.57%). Li Guolong et al. (2013) studied, development of nutrition enriched milk noodle. They stated that making use of protein interaction, the processing of nutrition enriched soybean milk noodle, which is added with soybean latex and found that protein content of nutrient enriched soybean milk noodle, was increased and its nutrition rises in value. Bashir et al. (2012) prepared pasta using semolina, chick pea flour and defatted soy flour at different levels. They observed as the concentration of legumes was increased the cooking time also increased. The cooking quality of the pasta was enhanced by steaming. On the basis of cooking and sensory quality, pasta containing 14% chickpea flour and 10% defatted flour resulted in better quality and nutritious pasta. Baskaran et al. (2011) analyzed sensory attributes of noodles supplemented with 5, 7.5 and 10 per cent skim milk powder. They reported that, the colour score as 7.67, 7.50, 7.83, flavour as 7.67, 8.00, 7.67, texture as 7.00, 6.67, 5.33 and overall acceptability as 6.83, 6.3, and 5.50. Sood et al. (2009) studied the quality of wheat: fruited pasta products made with wheat and blended with 20% jamun and papaya pulp. They reported that the colour score as 8.73, 8.19. 8.48, taste 8.52, 7.38, 8.81, consistency 8.02, 7.04, 7.18 and overall acceptability 8.42, 7.53 and 8.16. 14 Biswas (1999) reported significant decrease in the colour score of bread and biscuits by the addition of apple pumice, rice bran and soybean flour to wheat flour. Similarly, Sharma (2002) reported that decrease in the flavour score of noodle due to addition of maize flour in wheat flour but improve the texture score. Improvement in the texture of breads was also reported by Dogra, (1999). Salunkhe (1985) observed that Triticum duram (hard wheat) had high protein content and hard texture needed for the production of semolina, macroni and pasta products. 2.2 Basil seeds (Anonymous, 2013) reported that the sweet basil seeds do resemble the “chia” seeds and is known by the names sabja seeds or falooda seeds or Tukmaria in India. They are basically the seeds of the sweet basil. Sabja seeds are the most commonly used in making falooda and can be easily added to sherbets, milkshakes and other drinks. Although these seeds do not have taste of their own but they give a good texture to the dish and make it healthy too. Tucakov (1996) reported that sweet basil (Ocimmum basilicum L.) is the common medicinal and culinary herb, widely used in many traditional medicines. In our traditional medicine, basil has been used as potent antiseptic and preservative, slight sedative, digestive regulator and diuretic. It has also been recommended for the treatment of headaches, coughs, infections of upper respiratory tract and kidney malfunction 15 2.2.1 Nutritional and therapeutic importance of basil seeds (Anonymous, 2012) Stated that the Basil seeds add to authentic look and are actually very good for digestion in hot climate. 1. Basil seeds are taken in sherbats as they produce cooling sensation with the stomach. 2. Basil reduces the nauseous feeling & controls vomiting. 3. Basil seeds are good expectorants and when chewed they treat sore throat, cough, asthma, & headache. 4. Scientific studies have revealed that compounds in basil seed oil have potent anti-oxidant, anti-cancer, anti-viral and anti- microbial properties. 5. Sweet basil is spice and medicinal plant having oils, tannins, trepans, flavonoids, glycosides. 6. Basil is also source of macro and micro elements. Biesiada and Kus (2010) studied on “nutritional status of sweet basil”. They stated that, sweet basil is well known and appreciated spice and medicinal plant. Apart from biologically active compounds, such as volatile oils, tannins, terpens, flavonoids or glycosides, it is also a valuable source of macro and micro – elements. Sulochanamma et al (2009) reported that the sweet basil or tulasi have bright green leaves with strong smell. Basil has many bio active properties such as anti–microbial, anti– inflammatory and anti-allergic due to presence of compounds in 16 the volatile oil from its leaves. It is useful in respiratory tract infection. The ursolic acid present in basil has anti – allergic properties. It is also anti-inflammatory due to oil eugenol present in the leaves. The plant can also play a role in the management of immunological disorders such as asthma. Beric (2008) studied Mutagenic and anti-mutagenic properties of essential oil of basil and its major constituent linalool, reported to possess anti oxidative properties, were examined in microbial tests. Chattopadhay et al. (1999) stated that, numerous laboratory studies have shown various effects of Ocimum sp., including bactericidal, anti diarrheal, chemo preventive, bloodsugar lowering, a nervous system stimulatory and radiation protection. Aruna and Sivaramkrishnan (1992) studied the anticarcinogenic properties of basil leaves (Ocimum sanctum L.) on mice and rats with carcinoma. They suggested that basil leaves, which are widely used in Indian cooking, may prove to be valuable anti-carcinogenic agents. In addition to antioxidative, anticarcinogenic and antimutagenic substances, basil contains also anti microbial compounds, one of them being eugenol (Meena and Vijay 1994). Anti-microbial activity of sweet basil has been found at least against such as Lactobadllus cerevisiae, and Mycoderma sp. acidophilus, Saccharomyces 17 2.2.2 Use of basil seeds Khazaei and et al. (2014) investigated the use of basil seed gum (BSG) as a new film –forming material under the influence of addition of glycerol as plasticizer. Edible films based on BSG and glycerol was developed, and their water vapour permeability, as well as physical, thermal, and mechanical properties was measured. This study revealed that the BSG had a good potential to be used in producing edible films for various food applications. Mathews et al. (1993) made a preparation called “falooda”, which contains basil seeds with water or milk made as thickened mucilaginous food. Askerova et al. (1993) studied many alcoholic beverages, for example bitters, liquors, and spirits, containing basil. Russians have patented a method to improve the storage stability and organoleptic properties of a carbonated fermented milk beverage by adding a mixture of essential oils of coriander, basil and fennel to a salt solution of whey. Bagaturiya et al. (1991) reported that, Russians have patented a method to prepare a non alcoholic beverage concentrate even from the residues of grapes and high – eugenol basil. Rakauskas and Sudzhene (1979) reported that, sweet basil, its extracts and oils are widely used in combination with other spices and herbs in confectionary products, sweets, bakery products, puddings, condiments, vinegars, ice creams, mustard and in pickled vegetables. Basil is common ingredient in spice 18 mixtures. Russians have patented basil containing salt substitute without sodium salts for dietic use. Rice noodles and vegetables get a fine aroma when cooked in herb liquid. The liquid is prepared by simmering herbs in water. Turtle soup, oxtail soup and the French soup paysanne contain basil as one of the essential ingredients. Basil goes well also with cheese and cheese dishes. Reuter (1976) reported that basil is recommended to flavour beef stews and bouillons, pork, meat loaf, meat balls and shish kebabs, lamb dishes and inner organ‟s dishes, as well as chicken in Turkey. Canned beef stew tastes homemade when basil is added. Basil suits all fish dishes and it may also be used in other seafood dishes. 2.2.3 Chemical composition of basil seeds Holland et al. (2012) concluded that, food composition tables 100 grams of fresh basil leaves contain 26 mg of vitamin C, 3.95 mg of carotene, 0.08mg of thiamin, 0.31mg of riboflavin, 1.1 mg of niacin. Table 2. Chemical composition of basil seeds Biesiada et al. (2010). Constituent Per cent Energy Fat Protein CHO Dietary fiber Water 94 KJ 0.64 3.15 2.65 1.6 92.06 Chen et al. (1993) reported, the β- carotene content of sweet basil is nearly twice that of carrot. The seeds of Ocimmum 19 basilicum L. are high in fiber and they can be considered as new source of dietary fiber. 2.3 Falooda Patel and Arora (2005) studied fiber fortification of dairy products. They stated that most dairy products do not contain dietary fiber; there are a few dairy foods which contain non –dairy ingredients contributing to various amounts of fiber. Fiber reach sources may be wheat bran, sorghum meal, oat meal, soya bean hulls, fruits and vegetables. They stated falooda is a milk-based drink, flavored with rose syrup, added with strands of corn flour and vermicelli. The whole corn flour and the toppings containing cherries or dried papaya contribute to its dietary fiber content. 2.3.1 Chemical composition of falooda Kabir et al. (2013) developed feeding manual - 2013 for Bangladesh, the100 g fruit falooda contains. Table 3. Chemical composition of falooda Constituent Ash Moisture Protein Fats and oils Energy Carbohydrates Zinc Iron Calcium Vitamin 2.4 Similar products Values 1.10 g 64.12 g 4.54 g 5.90 g 168.62 calories 24.34 g 0.54 mg 0.27 mg 170.1 mg 60.380 (IU/100g) 20 Gavhane et al. (2015) stated that lassi was prepared from cow milk with constant sugar level (10%) and water (20%) and different levels of finger millet flour (2%, 3% and 4%). The product prepared using 3% finger millet flour was found to be most acceptable on the basis of overall acceptability. Changade et al. (2012) reported that bottle guard kheer had greenish shade while pumpkin kheer had yellowish shade. The shreds and cubes of both vegetables become soft during cooking which imparts characteristics chewy texture to the vegetable based kheer. All vegetable based kheer sample tasted low level of fat and high level of carbohydrate content. The total solid and carbohydrate content was higher in kheer prepared from bottle gourd while protein was higher in control and kheer prepared from pumpkin. Mehta (2011) studied flight kitchen and food practices and estimated the composition of 100 g vermicelli kheer as moisture-56.38g, energy-119Kcal, protien-2.68 g, fat-5.34 g, carbohydrates-15.18 g. Gajbhiye et al. (2007) reported that, doda burfi is one of the popular traditional Indian cereal based milk product of northern India. Doda burfi is made from sanmak (pretreated wheat grains), wheat flour, sugar and milk. Kumar et al. (2007) stated that, phirni is cereal based indigenous milk product, commonly found in western UP and Hydrabad but popular in country wide. Payssam, Kheer, Basundi are similar product profile of phirni. 21 Qureshi et al. (2007) studied, the manufacturing techniques of sterilized carrot kheer wherein he stated that, for preparation of carrot kheer shredded carrot was cooked in presence of ghee to develop characteristic flavour. Other ingredients like milk, sugar and dry fruits were added to it and the whole mass was cooked till desired consistency was obtained and then sterilized. Shredded carrot was added at three different levels of 20, 30, and 40 per cent with 8 per cent sugar. Basu et al. (2007) stated, a method of development of cereal based milk product, using skim milk and un-germinated pearl millet (Pennisetum glycum) flour as raw materials and cumin, black pepper, curry patta and salt as flavouring agents. Patel and Arora, (2005) Stated that several traditional Indian dairy products contain various cereals and legumes as ingredients. Kheer and payasam are prepared using rice, sevian (vermicelli), makkana (lotus puffs) sago and nuts (almonds, cashew nuts and pistachio). Paysam may also contain vermicelli, kaddu (pumpkin), poppy seeds (khus- khus), bengal gram dal, green gram dal, bitten rice, suji, cooked rice, mango and jack fruit. Sohan halwa is another germinated wheat based buffalo milk product popular in north India. It has an extremely chewy texture, which is attributed to the simultaneous presence of wheat glutein and casein. Also, ghevar, milk sweet prepared from admixture wheat flour, is a rare delicacy of Rajasthan De et al. (1976) reported that, Kheer is an Indian dessert prepared by partial dehydration of whole milk in open 22 pan over direct fire with sugar and usually rice or other cereals, which is relished by one and all throughout the country. 23 3. MATERIAL AND METHODS This chapter deals with material and methods used for manufacture of falooda and analytical methods used for testing of raw materials and finished product. The research work on “Standardisation of falooda” was conducted in laboratory of Department of Animal Husbandry and Dairy Science , Post Graduate Institute , MPKV, Rahuri. 3.1 Material Following materials were used in the laboratory for analytical work. 3.1.1 Glassware Borosil and corning glass wares were used to analyze milk and falooda. 3.1.2 Electrical balance Electronic precBISon balance (BT 2245, Sartorius ISO 9001) was used for weighing of samples, ingredients and chemicals, etc. throughout the study. 3.1.3 Muffle furnace “Tempo” make muffle furnace was used for determination of the ash content in falooda samples. 3.1.4 Mixer /Grinder “Bajaj FX 10” food processor was used for grinding the samples. 3.1.5 Vessel Stainless steel vessel along with stainless steel laddle was used for preparation of falooda. 24 3.1.6 Chemicals All the chemicals used in study for the analytical purpose were of analytical (AR) and guaranteed reagent (GR) grade by Merk, India Ltd and Glaxo India Ltd. 3.2 Ingredients 3.2.1 Milk The fresh, clean, composite milk was procured from Research cum Development Project on cattle (RCDP), Department of Animal Husbandry and Dairy Science, MPKV, Rahuri. 3.2.2 Sugar The sugar was purchased from local market, Rahuri. 3.2.3 Vermicelli The wheat vermicelli used in research was purchased from local market, Rahuri. 3.2.4 Basil seeds The basil seeds used in research were purchased from local market, Rahuri. 3.2.5 Dry fruits The dry fruits (Almonds, Cashew nuts and Pistachio) used in research were purchased from local market, Rahuri. 3.2.6 Cherry The cherries used in research were purchased from local market, Rahuri. 25 3.3 Methods Various methodological and analytical methods adopted in the preparation and testing of raw materials and falooda are outlined in the section. 3.3.1 Chemical analysis of Milk Collected composite milk from Research cum Development project on cattle was analyzed during every replication for different chemical constituents by adopting standard procedures given below. 3.3.1.1 Fat The fat content was determined by Gerber‟s method as per procedure described in BIS (1981). 3.3.1.2 Total Solids The total solids content was determined by gravimetric method as per BIS (1981). 3.3.1.3 Acidity (% LA) The titerable acidity was determined as per the procedure described in BIS (1981). 3.3.1.4 Protein The protein content was determined by Micro- Kjeldahl‟s method as described in BIS (1981). The per cent nitrogen was multiplied by 6.38 to find out the protein percentage in milk. 3.3.1.5 Lactose The lactose content was determined by Lane - Eynon volumetric method described in BIS (1981). 26 3.4 Pre-experimental trials Pre-experimental trials were conducted to decide the levels of vermicelli, basil seeds, sugar and rose syrup to be used in the final experiment during falooda preparation. The details of pre-experimental trials are mentioned as below 3.4.1 Selection of levels of different ingredients in pre-experimental trials During pre-experimental trials various combinations of vermicelli, basil seeds, sugar and rose syrup were tried in combinations or separately with various levels. 3.4.2 Selection of levels of Vermicelli and Basil seeds Different lots of falooda were prepared in the laboratory using different levels of the vermicelli and basil seeds combinations. Rests of the combinations were maintained uniform. The levels for the beaker trials were decided on the basic compositions of dry falooda mix of various brands available in the market. In the first phase of the pre- experimental trials, three levels of vermicelli (3%, 4% and 5%) and three levels of basil seeds (0.2%, 0.3% and 0.4%) were tried, with 10 per cent constant sugar level. Thus total of nine combinations were tried twice and also subjected to sensory evaluation by the semi trained judges using the 9 point hedonic scale. From nine treatment combinations few combinations were deleted on the basis of sensory evaluation results and discussion made. 27 In the second phase of the pre –experiential trials, on the basis of results of first phase, three levels of vermicelli (3.5%,4.5% and 5.5%) and three levels of basil seeds (0.5%, 0.6% and 0.7%) were tried with 12 % constant sugar level. Thus total of nine combinations were tried twice and also subjected to sensory evaluation by the semi trained judges using the 9 point hedonic scale. From nine treatment combinations few combinations were deleted on the basis of result of sensory evaluation and discussion made. Thus, total of eighteen combinations were prepared, out of which two of the best treatments were selected on basis of high scores in the sensory evaluation. Top two levels from phase –I and phase –II were then tried with the two levels of sugar (10% and 12%). Thus eight combinations were then subjected to sensory evaluation by the semi trained judges using 9 point hedonic scale. The judges showed more inclination towards falooda prepared using 3.5% and 4% of vermicelli and 0.4% and 0.6% of basil seeds. Later it was decided to discard remaining levels and finally three levels of vermicelli (3%, 3.5% and 4%) and two levels of basil seeds (0.4% and 0.6%) were selected for final experimental trials. These pre-experimental trials were essential to design main experiment, which helped to draw valid conclusions for selecting proper levels of ingredients. 28 3.4.3 Selection of levels of sugar and level of reduced milk In market falooda, the consistency of the product was observed to be too thin and taking in to consideration that additional moisture is naturally going to be added to product through cooked vermicelli and water soaked basil seeds, it was discussed and decided that, milk should be reduced to 50% of concentration. Pre-experimental trials were conducted to decide combinations of sugar. This was done by keeping one or other factor constant with various grades of factor to be optimized. Thus total of eighteen combinations of vermicelli and basil were tried, out of which four best combinations were compared with two levels of sugar (10% and 12%). On the basis of sensory score, 10% level of sugar was finalized for further trials. 3.4.4 Level of dry fruits The dry level of dry fruits was decided according to (Kankhare et al, 2012) who conducted research trial on “Standardization of Mastani-a milk based product”. 3.4.5 Selection of level of Rose syrup and Cherries Rose syrup is commonly observed in market falooda. Therefore, trials were conducted to determine the amount of rose syrup and cherry to be used in preparation of falooda. One per cent and 2 per cent each of rose syrup and cherries were tried. And on the basis of sensory evaluation (as per, 9 point hedonic scale), the level of rose syrup and cherries was finalized at 1 per cent of whole milk. 29 3.5 Final Experimental trials On the basis of the results of sensory evaluation of pre-experimental trials, the levels of vermicelli and basil seeds were finalized for inclusion in the final experimental trials. Dry fruits, sugar, rose syrup, condensed milk and cherries at predecided levels were used in falooda preparation. Different lots of falooda were prepared using finalized levels and were evaluated for sensory and chemical attributes. 3.5.1 Treatments details: Table 4. Treatment Treatments details Levels of vermicelli and basil seeds Replications 1 T1 : V1B1 3.0 per cent vermicelli of whole milk and 0.4 per cent basil seed of whole milk. T2 : V1B2 3 per cent vermicelli of whole milk and 0.6 per cent basil seed of whole milk. T3 : V2B1 3.5 per cent vermicelli of whole milk and 0.4 per cent basil seed of whole milk. T4 : V2B2 3.5 per cent vermicelli of whole milk and 0.6 per cent basil seed of whole milk. T5 : V3B1 4.0 per cent vermicelli of whole milk and 0.4 per cent basil seed of whole milk. T6 : V3B2 4.0 per cent vermicelli of whole milk and 0.6 per cent basil seed of whole milk. 2 3 4 30 Sugar @ 10 per cent of milk, dry fruits @ 2 per cent of milk and rose syrup @ 1 per cent of milk was used for all treatments. 3.5.2 Technique for preparation of falooda The composite, whole milk was taken in a stainless steel container. It was preheated to 350C, then filtered and heated to obtain 50 % condensation of original volume of milk. Sugar at 10 % of raw milk was mixed with above condensed milk. The cooked and soft vermicelli were then added to it. The water soaked basil seeds were then added to condensed milk. Dry fruits @ 2% of whole milk, out of which 70% of it were then added as cut pieces in to the above mixture. Rose syrup @ 1% was then mixed thoroughly with the product. Product is now garnished with the chopped dry fruits (30% of 2%) and cherry @ 1% of whole milk. Product was then refrigerated to attain temperature of 5-70c. The schematic preparation of falooda is as given in 3.5.3. flow diagram for 31 3.5.3 Flow chart Basil seeds (as per treatment) Soak in water for 30 min cow milk Enough water heating Drain the excess water Reduce volume to 50 % heating Add vermicelli (as per treatment) Keep the seeds in refrigerator Add sugar @ 10 % of milk simmer till they get Soft and cooked Add vermicelli & mix it well Add dry fruits pieces (2%) & mix it well (Whole pistachio, almond, cashewnut-70% of 2%) Add basil seeds & mix it Add rose syrup (1% of whole milk) Add pieces of dry fruit (Chopped pieces – 30% of 2%) Garnish it with cherries Cool it in refrigerator (5-7 0c) 3.5.4 Addition of ingredients 32 During preparation of faloodsa different ingredients were processed and were added in a proper sequence to make the product unique in taste. 3.5.4.1 Addition of vermicelli Locally available wheat vermicelli was used. Vermicelli were added to hot water which was about to boil. The quantity of water required per 100 g of vermicelli was about 700 ml. Stirring was done while, adding vermicelli to hot water. The mixture was kept at simmering temperature over a low fire with stirring to avoid scorching, till they get soft and cooked. Cooking was continued for 8 to 10 min. after achieving desired swelling and softness, excess water was drained off and cooked vermicelli was added to condensed milk. 3.5.4.2 Addition of basil seeds Locally available basil seeds were used. Basil seeds were soaked in enough water. The quantity of water required for 10 g of basil seeds was 200 ml. After 30 minutes of soaking the excess water was drained off and seeds were then added to mixture of vermicelli and condensed milk. 3.3.4.3 Addition of dry fruits The dry fruit viz; almond, cashew and pistachio were used in two forms viz, cut pieces (1/3rd or 1/4th cutting of one dry fruit) and thin, flat, chopped form. The dry fruits level was decided according to (Kankhare et al, 2012) who conducted research trial on “Standardization of Mastani-a milk product”. Dry fruits @ 2% of whole milk, out of which 70 % of it were then 33 added as cut pieces (1/3 rd or 1/4th cut pieces, depending on size) in to the product. 3.5.4.4 Addition of Rose syrup Rose syrup @1 per cent of whole milk was added and mixed thoroughly to above mixture. 3.5.4.5 Garnishing of the product After adding all ingredients, product was garnished with cherries at 1 per cent of whole milk and the chopped (thin, flat) dry fruit (30 % of 2%). 3.6 Sensory evaluation of falooda Falooda samples prepared under different pre- experimental and experimental treatments were subjected to sensory evaluation using the method described in the IS.6273, Part-I and Part- II (1971) by adopting 9 point hedonic scale. A panel of six semi trained judges was formulated for this purpose. The samples were coded every time to conceal their identity and were offered to the judges for evaluation of the quality attributes. 3.7 Chemical analysis of falooda 3.7.1 Fat The fat content was determined by Gerber‟s method as described in IS: 1224, Part I (1977). Exactly 10 gm of well mixed and grinded falooda sample was weighed in a glass beaker and 3 to 4 ml hot distilled water was added to make the liquid. 10 ml Gerber sulphuric acid (90:10, Acid: distilled water) was transferred into the same butyrometer and add prepared liquid of falooda 34 sample followed by 1 ml iso- amyl alcohol. Liquid level in the butyrometers was brought to calibration by addition of required amount of distilled water. The butyrometer was lock stoppered and the contents were vigorously shaken to digest non fat substances. It was then centrifuged in Gerber centrifuge machine for 5 min. fat column was noted after tempering butyrometer for 5 min. in water bath maintained at 65oC and then recorded the fat per cent of falooda samples. 3.7.2 Protein Protein content was determined by Micro Kjeldahl‟s method as described by Chaudhari, (1959). Two grams of grinded falooda sample was taken in Kjeldhal‟s flask. It was added with 1g of salicylic acid, 20 ml H2SO4 and one tea spoon salt mixture (Potassium sulphate 10g + copper sulphate 2g). The Kjeldhal‟s flask was then kept for digestion for 8 to 10 hrs. After digestion, the digested sample was taken in round bottom flask. Then 200 ml distilled water was added into it and a piece of red litmus paper was also added. Then 100 ml 40% NaOH was added in it. The flask was then kept for distillation and the ammonia produced was collected in 20 ml boric acid. After adding 2-3 drops of methylene red indicator, the distillate was titrated with 0.1N NaOH till colour changed from red to yellow when end point reached. The per cent nitrogen in the sample was calculated by using following formula. 35 % nitrogen in Sample No. of ml of 0.1 N of NAOH x 0.0014 = ---------------------------------------------- x 100 Weight of sample % protein = % of nitrogen in falooda sample x 6.38 3.7.3 Lactose The lactose content was determined by Lane - Eynon volumetric method described in BIS (1981). The 25 g sample was taken into 100 ml volumetric flask and volume was made to 200 ml using distilled water. Then acetic acid of 10 % concentration was added into it. This was kept for 5 to 7 min. till complete precipitation occurred. Then it was filtered through Whatman No. 40 filter paper. The filtrate obtained was filled in a clean burette and was titrated against a mixture containing solution of Felhing‟s A (5 ml) and Felhing‟s B (5 ml) along with 2-3 drops of methylene blue as indicator with continues heating and shaking till blue colour turned to brick red (under boiling condition). The reading was recorded. The lactose was calculated as – Formula Lactose (%) 67.84 = ----------A Where, A = ml of amount of titrate from burette required to reduce Felhing‟s A and Felhing‟s B solution. 36 3.7.4 Ash Ash content was estimated as per the procedure given in BIS (1981). 10 g of grinded falooda sample was taken in a silica crucible for preliminary drying of sample. After the sample was placed in the muffle furnace and the temperature was brought to 5500C and held for 4 hr. The ash was obtained after cooling. 3.8 Statistical analysis The experiments were laid out in Two Factorial Completely Randomized Design (FCRD) with four replications. (Snedecor and Cochran, 1994). 3.9 Cost of preparation of falooda The cost estimation (Rs/Kg) of the newly formulated product was worked out by taking into account the prevailing market rates of the ingredients as well as other charges used during manufacture of falooda. (Lal et al, 1980). 37 4. RESULT AND DISCUSSION In the various research reports published, very little information is available on these aspects. Therefore research methodologies in this respect required to be pre-tested and standardized. The pre-experimental trials are already discussed in the chapter 3. The results obtained during the course of present investigation on “Standardization of falooda” in relation to the sensory and chemical attribute for different treatments are presented and collected data are tabulated, statistically analyzed and results of the present study have been discussed in this chapter under the different headings. 4.1 Chemical composition of milk The milk procured from Research cum Development Project, Rahuri was analyzed for its chemical composition. The results are tabulated in Table 5. Table 5. Chemical composition of whole milk Constituent Fat Protein Lactose Total solid Ash Acidity(% LA) The figures presented in Table 5. Per cent 4.0 3.43 4.45 12.53 0.65 0.13 Reveal that, milk used for preparation of falooda had on an average 4.0 per cent fat, 12.53 per cent total solids and 0.13 per cent acidity. Similarly protein, lactose, and ash content were observed to be 3.43, 4.45 and 0.65 respectively. 38 4.2 Chemical composition of falooda Chemical analysis of fresh falooda was carried out for estimation of fat, protein, lactose and ash. The results are furnished under the heads from 4.2.1 to 4.2.4. 4.2.1 Fat Table 6. Effect of treatment combinations on Fat (%) content in falooda Parameter Treatments Fat B1 B2 Mean V V1 7.19 7.21 7.20 V2 7.27 7.28 7.27 V3 7.31 7.32 7.31 Mean B 7.26 7.27 7.26 Factors V B VxB SE 0.003 0.002 0.004 CD at 5% 0.008 0.007 NS From perusal of data the fat content in falooda varied within a narrow range of 7.19 to 7.32 per cent. The vermicelli and basil seeds had little fat content. From above table, it is observed that, fat content in treatment samples of falooda slightly increased with increase in levels of vermicelli and basil seeds. Treatment V1B1 (with addition of 3 percent of vermicelli and 0.4 percent of basil) showed lowest fat content (7.19) whereas; treatment V3B2 (with addition of 4.0 percent of vermicelli and 0.6 percent of basil) had highest fat content (7.32). 39 The above observations indicate that, the levels of vermicelli and basil seeds had significant effect on the fat content of different falooda samples. But the interaction (V x B) Effect of different levels of vermicelli and basil on fat content was non significant. Since the levels of condensed milk and dry fruits were kept constant and only levels of vermicelli and basil seeds varied in treatments and both the said ingredients contained very low fat which resulted in to non significant interaction effect. Patel and Upadhyay (2003a) observed the fat content of basundi by using buffalo milk as 10.40%, 10.38% and 10.42%. Patel et al., (2004) studied the influence methods of milk concentration on physico chemical properties of basundi. They reported higher values of fat as 11.73%. 11.70%, 11.50% and 40 4.2.2 Protein Table 7. Effect of treatment combinations on Protein (%) content in falooda Parameter Treatments Protein B1 B2 Mean V V1 9.61 9.64 9.63 V2 9.82 9.85 9.84 V3 10.03 10.05 10.04 Mean B 9.82 9.85 9.83 Factors V B VxB SE 0.007 0.006 0.01 CD at 5% 0.022 0.018 0.03 The influence of experimental treatments on protein content in the falooda samples was significant. The mean values for protein in V1B1 , V1B2 , V2B1, V2B2, V3B1, and V3B2 were 9.61, 9.64, 9.82, 9.85, 10.03 and 10.05 per cent respectively. The highest value for protein content was 10.05 per cent in the treatment V3B2. The increase in the protein content was observed with increase in the level of vermicelli. It might be due to protein present in the vermicelli. All the treatments significantly (P<0.05) differed among themselves. The above observations indicate that, the levels of vermicelli and basil seeds had significant effect on the protein content of different falooda samples. Interaction (V x B) effect of 41 vermicelli and basil seeds, on protein content was also significant. Similar findings were reported by Patel and Upadhyay (2003a). They observed the proximate composition of basundi as, 7.81%, 7.39% and 7.06% of protein. The average values for Protein content in falooda are more or less similar to the figures reported by Patel and Upadhyay (2003b) as 9.55, 9.03 and 8.88% of protein for basundi prepared with 5, 6 and 7 per cent sugar. The protein content observed here agrees with the values reported by Patel et al., (2004). 4.2.3 Lactose Table 8. Effect of treatment combinations on Lactose (%) content in falooda Parameter Treatments Lactose B1 B2 Mean V V1 10.03 10.05 10.04 V2 10.10 10.12 10.11 V3 10.18 10.20 10.19 Mean B 10.10 10.12 10.11 Factors V B VxB SE 0.003 0.002 0.004 CD at 5% 0.009 0.007 NS The data pertaining to the lactose content of falooda are presented in Table above. The perusal of table 8. indicates 42 that, the average lactose content was 10.11 per cent. All experimental treatments differed significantly among themselves (P<0.05). It was noticed that as the level of vermicelli and basil seeds in the falooda increases, the lactose in the sample treatments increased very slightly. However the slight increase over the treatments might be due to high CHO content in the vermicelli. The above table shows that, lowest lactose content (10.03) was found in V1B1 combination where as highest content was found in V3B2 combination. The above observations indicate that, the levels of vermicelli and basil seeds had significant effect on the lactose content of different falooda samples. But the interaction (V x B) effect of different levels of vermicelli and basil on lactose content was non significant. It might be due to fact that, the lactose being only present in the milk, which remained constant over the treatments. Similar findings were reported by Patel and Upadhyay (2003a). They observed 10.44%, 10.88% and 10.66% of lactose in basundi. Patel and Upadhyay (2003b) prepared basundi from standardized buffalo milk using three levels of sugar viz; 5, 6, 7%. They found the proximate composition as, 9.73, 9.81, and 9.68% of lactose. Patel et al., (2004) reported very little higher values who studied the influence of methods of milk concentration physico 43 chemical properties of basundi. They reported 10.90%, 10.38% and 10.28%, of lactose 4.2.4 Ash Table 9. Effect of treatment combinations on Ash (%) content in falooda Parameter Treatments Ash B1 B2 Mean V V1 1.15 1.16 1.15 V2 1.19 1.21 1.20 V3 1.24 1.25 1.24 Mean B 1.19 1.20 1.20 Factors V B VxB SE 0.004 0.003 0.005 CD at 5% 0.012 0.009 0.014 The data presented in table above, reveal that the ash content in the falooda samples under different experimental treatments due to addition of vermicelli and basil seeds showed significant (P<0.05) differences. The mean ash content ranged from 1.15 (V1B1) to 1.25 (V3B2) percent. The sample of the treatment V3B2 had significantly higher ash content (1.25) over the rest of the treatments where as the treatment V1B1 had significantly lower value (1.15 %). It was seen that with increase in the level of vermicelli and basil seeds in falooda, there was increase in the ash content in the falooda sample. The above observations indicate that, levels of vermicelli and basil seeds 44 had significant effect on the ash content of different falooda samples. Interaction (V x B) effect of vermicelli and basil seeds, on ash content was also significant. Similar findings were reported by Patel and Upadhyay (2003a), observed the proximate composition of basundi as, 1.26%, 1.39% and 1.38% of ash, Patel and Upadhyay (2003b) prepared basundi from standardized buffalo milk using three levels of sugar viz; 5, 6, 7%. They reported ash values as 1.68 and 1.67%. Simalry Patel et al., (2004) also reported the same values for ash. De (1980) reported the chemical composition of kheer as under: Table 10. Constituent Kheer (Per cent) Moisture 67.02 Fat 07.83 Protein 08.34 Lactose 08.54 Ash 01.41 Sugar 08.95 45 4.4 Sensory evaluation of falooda In any product development process, sensory attributes play a vital role in deciding the acceptability of the product. The sensory parameters chosen to assess the falooda with different combinations were flavour, colour and appearance, consistency and overall acceptability. The results obtained for sensory evaluation of falooda are discussed as under. 4.4.1 Colour and appearance Table11. Effect of treatment combinations on Score for colour and appearance of falooda samples Parameter Treatments Colour and appearance B1 B2 Mean V V1 6.83 7.13 6.98 V2 7.33 7.63 7.48 V3 7.56 7.40 7.49 Mean B 6.98 6.98 7.31 Factors V B VxB SE 0.054 0.044 0.077 CD at 5% 0.170 0.138 0.240 From Table, it is revealed that the mean sensory scores for colour and appearance of the product under different treatments were significant. It means that the addition of different levels of vermicelli and basil seeds in the falooda 46 samples affected the colour and appearance of the falooda. It was observed that the mean colour and appearance score for the experimental treatments V1B1 , V1B2 , V2B1, V2B2, V3B1, and V3B2 were 6.83, 7.13, 7.33, 7.63, 7.56 and 7.40 respectively. The treatment V2B2 had the highest score (7.63) for colour and appearance and the treatment V1B1 had lowest score (6.83). The high score was noted for V2B2, this might be due to floating of basil seeds which disperse on the surface, which was more appealing to judges compared to other combinations. As treatment V2B2 contains high per cent of basil seeds i.e. 06 per cent. But this case was not observed in V3B2 , where the basil seeds per cent ages were same. But due to high amount of vermicelli in V3B2, which gave a thick bodied appearance to product in turn, resulted in to low score. Addition of basil seeds resulted in attractive colour and shiny appearance to the surface of the product. From the given table, it is seen that higher level of the basil seeds improved scores for this attribute considerably. Basil seed had white tinge on the outer surface with inner glossy translucent black colour. The above observations indicate that, the levels of vermicelli and basil seeds had significant effect on the colour and appearance of different falooda samples. Interaction effect of vermicelli and basil seeds, on this sensory attribute was also significant. 47 The present results obtained were in agreement with Biswas (1999). He reported that, addition of soy flour in biscuits and bread decreased score for colour and appearance as compared to control biscuits and bread. Baskaran et al. (2011) also analyzed increasing colour attributes of noodles supplemented with skim milk powder. Aneja et al. (2002) stated that kheer prepared from buffalo milk was whiter and thick bodied and is therefore preferred over that obtained from cow milk. In addition to milk, kheer also contained substantial amount of non-dairy ingredients such as rice, sugar, sabudana, semolina, etc as well as nuts and spices. It was characterized by sweet, nutty and pleasant flavour that was highly acceptable. Colour of the product generally depends on the extent of heat treatment given and may range from almost white to slightly brownish. However a rich creamy shade of kheer was highly preferred. 48 4.4.2 Flavour Table12. Effect of treatment combinations on Score for flavour of falooda samples Parameter Treatments Flavour B1 B2 Mean V V1 6.90 7.26 7.08 V2 7.46 7.70 7.58 V3 7.50 7.30 7.40 Mean B Factors 7.28 V 7.42 B 7.35 VxB SE 0.054 0.044 0.077 CD at 5% 0.170 0.136 0.240 The highest score of (7.70) was obtained by the V2B2 falooda sample prepared from 3.5 percent of vermicelli and 0.6 percent of basil seeds followed by V2B2, V3B1, V3B2, V1B2 and V1B1 samples. All samples differed significantly from each other in their influence on the flavour of falooda. Score for the flavour is the combined effect of taste and smell. As level of vermicelli increases the smell of cooked vermicelli becomes denser. So lower score were noted in samples (V3B1 and V3B2) contained high vermicelli level. The high score for V2B2 is might be due to the balanced effect of smell and taste over other combinations. Above table indicates that, the levels of vermicelli and basil seeds caused significant difference in flavour of the product and also the interaction of levels of vermicelli and basil seeds (V X B) had significant effect on flavour of the product. 49 Present results are in agreement with Sharma (2002). He reported that addition of wheat flour in noodle decreased score for flavour. Similarly, Baskaran et al. (2011) analyzed flavour attributes of noodles supplemented with skim milk powder. He reported flavour score as 7.67, 8.00 and 7.67. Mogra and Midha (2013) prepared vermicelli using whole wheat flour, malted wheat flour and malted wheat flour + green gram dhal + spinach + sago flour. They observed decreasing trend in flavour score. Kadam et al. (2011) reported that flavour is blend of odour and taste which has great influence on the quality of the product. The results of the investigation indicated that flavour of reconstituted kheer was influenced by whole milk powder, basmati rice and sugar. 4.4.3 Consistency From (Table 6), it is revealed that the mean scores for consistency of the product under different treatments were significant. It was observed that mean score for the consistency of treatments V1B1 , V1B2 , V2B1, V2B2, V3B1, and V3B2 were 6.60, 6.90, 7.20, 7.60, 7.40 and 7.10 respectively. The combination V2B2 had the highest score (7.60) and the combination V1B1, had lowest score (6.60). Vermicelli contributes to the total solid; hence as there is increase in the vermicelli levels the consistency of the product increases. Therefore, in the V3B1,V3B2 combinations the product becomes too thick and so it becomes less acceptable. 50 Table13. Effect of treatment combinations on Score for consistency of falooda samples Parameter Treatments Consistency B1 B2 Mean V V1 6.60 6.90 6.75 V2 7.20 7.60 7.40 V3 7.40 7.10 7.25 Mean B 7.06 7.20 7.13 Factors V B VxB SE 0.047 0.038 0.067 CD at 5% 0.147 0.120 0.208 The above table indicates that, the levels of vermicelli and basil seeds caused significant difference on consistency of the product and also the interaction of, levels of vermicelli and basil seeds (V X B) had significant effect on consistency of the product. Kadam et al. (2011) observed that when there is increase in whole milk powder in kheer ready mix the consistency of the reconstituted kheer was not increased significantly. Increase in the consistency might be due to more colloidal nature of the rice. 51 4.4.4 Overall acceptability Table14. Effect of treatment combinations on Score for overall acceptability of falooda samples Parameter Overall acceptability Treatments B1 B2 Mean V V1 7.20 7.30 7.25 V2 7.60 8.00 7.80 V3 7.86 7.73 7.81 Mean B 7.55 7.67 7.61 Factors V B VxB SE 0.064 0.052 0.090 CD at 5% 0.199 0.161 0.281 The overall acceptability score of different falooda samples ranged from 7.20 to 8.00. The highest overall acceptability score (8.00) was observed in treatment V2B2 (3.5 percent of vermicelli and 0.6 percent of basil seeds) over rest of the sample treatments. The overall acceptability score of falooda samples differed considerably, due to levels of vermicelli and basil seeds. The high score might be due to perfect combination of level of vermicelli and basil seeds which results in to good combination of flavour, colour and appearance and desired consistency. All the sample treatments significantly differed among themselves and also, the interaction of vermicelli and basil had significant effect on overall sensory characteristics of the finished product. 52 The present results are in confirmation with Mogra and Midha (2013) reported the overall acceptability score ranged between 7.4 to 8.1 in vermicelli using whole wheat flour, malted wheat flour and malted wheat flour + green gram dhal + spinach + sago flour. Sood et al. (2009) also reported that the overall acceptability score ranged from 8.42 to 8.16 in wheat: fruited pasta. Bashir et al. (2012) prepared pasta using semolina (84%), chick pea flour (10%) and defatted soy flour (6%), semolina (76%), chick pea flour (14%) and defatted soy flour (10%) and semolina (68%), gram flour (18%), defatted soy flour 14%). They reported that overall acceptability ranged between 3.5 and 8.2. Kadam et al. (2011) reported that in case of ready mix kheer, the increase in the proportions of rice, whole milk powder, or sugar resulted in the lower scores for overall acceptability. Qureshi et al.(2007) developed and standardized sterilized carrot kheer with three different levels of shredded carrot were added 20, 30 and 40 per cent with 8 per cent sugar. They reported that the carrot kheer containing 30 per cent shredded carrot was preferred most by the panel. The overall acceptability of the fresh sample of carrot kheer was 7.5. 53 4.5 Cost of preparation of falooda The cost of production of falooda was worked out by taking in to consideration the prevailing retail market prices for the various items involved. The cost of preparation of falooda of treatment V2B2 (3.5 % vermicelli and 0.6% basil seeds) which found superior over all treatments was worked using the method of Lal et al. (1980) .The results are presented in Table15. Table 15. Cost of preparation of falooda. Sr. No Particulars Cost (Rs/Kg) 1 Milk 30.00 2 Sugar 30.00 100 g 3 3 Vermicelli 80.00 35 g 2.8 4 Basil seeds 100.00 6g 0.6 5 Dry fruits 3000 20 g 15 6 Rose syrup 150 10 ml 1.5 7 Cherry 100 10 g 1 8 Miscellaneous (for 1 kg): Depreciation of utensils, refrigeration, muslin cloth, fuel charges,etc. 9 Labour charges(for hrs) Total falooda cost V2B2 (3.5 % vermicelli and 0.6% basil seeds) Quantity Amount (Rs.) 1Kg. 30 - - 15 - - 20 760 g. 88.90 54 Cost of production of falooda of treatment V2B2 is estimated as Rs.88.90, i.e. Rs.89.00 for 760 g of falooda and Rs.119.00 for 1.0kg of falooda. 55 5. SUMMARY AND CONCLUSION The present investigation entitled, “standardisation of falooda” was carried out mainly to standardize the technique of the falooda. It is necessary to generate basic data on such traditional products which will help for designing of new equipments or for intelligent selection of existing food processing and packaging lines (Patil, 2006). Proper Standardisation will help to bring trade of traditional dairy products from small scale to large scale. 5.1 Pre- experimental trials Pre-experimental trials were conducted to select the levels of vermicelli and basil seed for the final experiment. Pre – experimental trials were also conducted to select levels of sugar, rose syrup and cherries. Composite milk used in this study contained on an average 4.0, 3.43, 4.45, o.65 and 12.53 per cent, fat, protein, lactose, ash, total solids, respectively. The S.N.F content was 8.75 per cent. Dry fruits (almond, cashew nuts and pistachio) were added at 2% of milk. The levels of rose syrup and cherries were decided to add 1% of milk after conducting preliminary trials. The addition of 10% of sugar for preparing Falooda was finalized after the trials. The judges showed more inclination towards falooda prepared using 3.5% and 4% of vermicelli and 0.4% and 0.6% of basil seeds. So it was decided to discard remaining levels. And finally three levels of vermicelli (3%, 3.5% and 4%) and two levels 56 of basil seeds (0.4% and 0.6%) were selected for final experimental trials. Accordingly, falooda samples were prepared and evaluated for various quality (sensory and chemical) parameters. The results are summarized as below: 5.2 Chemical quality of falooda 1. Fat Fat content in Falooda samples differed significantly (P<0.05) but the interaction (VxB) effect of vermicelli and basil seeds was non significant. This may be due to low fat content in the vermicelli and basil seeds. The fat content of falooda was lowest in V1B1 (7.19%) combination while it was highest in V3B2 (7.31%) combination. 2. Protein Protein content in falooda samples differed significantly (P<0.05) and also the interaction (VxB) effect was significant. This might be due to high protein content in the vermicelli. The lowest protein content was observed in the combination V1B1 (9.61%) while the combination V3B2 (10.05%) had the highest. Protein content increases with the increase in the level of vermicelli and basil seeds. 3. Lactose Lactose content in falooda samples differed significantly (P<0.05) but the interaction (VxB) effect of the vermicelli and basil seeds used was non significant. This may be due to fact that lactose is only present in the milk. The slight increase over the treatments might be due to high CHO content 57 in the vermicelli. The lactose content of Falooda was lowest in V1B1 (10.03%) combination while it was highest in V3B2 (10.12%) combination. Lactose content slightly increases with the increase in the level of vermicelli and basil seed. 4. Ash The ash content in the falooda samples under different treatments due to addition of vermicelli and basil seeds showed significant (P<0.05) differences and also the interaction (VxB) effect was significant. The sample of the treatment V3B2 had higher ash content (1.25%) over the rest of the treatments where as the treatment V1B1 had lower value (1.15%). Ash content increases slightly with the increase in the level of vermicelli and basil seeds. 5.3 Sensory evaluation of falooda 1. Levels of vermicelli and basil had significant effect on colour and appearance. Vermicelli at 3.5% and basil seeds at 0.6% was found significantly superior over other combinations. The effect of interaction of vermicelli and basil was also found significant. Combination V2B2 (Vermicelli at 3.5% and basil seeds at 0.6%) was statistically superior over others. This indicates that the levels were beneficial for falooda preparation. 2. The levels of vermicelli and basil seeds individually and in interaction (VxB) had significant effect on flavour of falooda. Combination V2B2 (Vermicelli at 3.5% and basil seeds at 0.6%) secured highest score compared with other treatments. Therefore, it can be stated that addition of vermicelli (3.5%) and 58 basil seeds (0.6%) is beneficial for flavour development in falooda. 3. Similar to above, individual and combined interaction effect of vermicelli and basil seeds was significant on consistency. Most samples were graded nearer to “liked very much”. Considering all sensory attributes together, the falooda sample of combination V2B2 (Vermicelli at 3.5% and basil seeds at 0.6%) proved to be most acceptable as they scored highest for the most important sensory attribute. 5.4 Cost of production of falooda The cost of production of falooda for treatment V2B2 (3.5 % vermicelli and 0.6% basil seeds) which found superior over all treatments was worked using the method of Lal et al. 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APPENDIX DEPARTMENT OF ANIMAL HUSBANDRY AND DAIRY SCIENCE Post Graduate Institute, Rahuri Evaluation card for sensory score of experimental samples (A specimen of evolution card) Date: Standardisation of falooda 9-point hedonic scale Name of evaluator : Designation : Code for the sample Flavour Colour and Consistency Overall appearance acceptability A B C D E F Remark: Time Signature of the evaluator 70 The numerical values of the score card as under (9-point Hedonic Scale). Maximum score 9 for each parameter Quality grade distribution Score 1. Like extremely - 9 2. Like very much - 8 3. Like moderately - 7 4. Like slightly - 6 5. Neither like nor dislike - 5 6. Dislike slightly - 4 7 Dislike moderately - 3 8. Dislike very much - 2 9. Dislike extremely - 1 71 8. VITA Mr. MORE VIKRANT SAHADEO Candidate for the degree of MASTER OF SCIENCE IN DAIRY SCIENCE 2015 Title of the thesis : Standardizations of falooda Major field : Dairy Science Biographical information : Personal : Born at Dongargaon, Tal. Mangalwedha, Dist. Solapur, on 23 August, 1991, Son of Shri. Sahadeo Shivaji More and Smt. Sita Sahadeo More Education : Passed S.S.C. Exam from Sainik School Satara (2007). : Passed H.S.C from Sainik School Satara (2009). : Received B.Sc (Agri.) Hon's degree with I class (2013) from College of Agriculture, Kolhapur. Permanent address : A/P – Dongargoan, Tal- Mangalwedha, Dist – Solapur Pin – 413305 E-mail address : [email protected]
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